Categories

  • About
    • BSG Advantage
      • Consumable
      • Enrichment/Depletion
      • Functional Integrity
      • On-Bead Digestion
    • Business Development
    • Corporate Offices
    • News & Events
    • How to Order
  • Products
    • Hemoglobin Removal Kits
      • Hemoglobin Removal/Depletion Kits
        • HemogloBind™ Hemoglobin Deletion From Hemolyzed Serum/Plasma
        • HemoTrial™ Kit
        • HemoVoid™ - Blood Card Reagent
        • HemoVoid™ - Hemoglobin Depletion From Erythrocytes
        • HemoVoid™ - Hemoglobin Variant Enrichment From Blood
        • NuGel-HemogloBind™
      • On-Bead Sample Prep Kit for LC-MS
        • HemoVoid™ LC-MS On-Bead
    • Lipid Removal & Clarification
      • Lipid Adsorption & Clarification Reagent
        • Cleanascite™ Lipid Removal Reagent and Clarification
    • Urine Protein & Low Abundance Enrichment
      • Albumin Depletion Kits from Serum or Plasma
        • AlbuVoid™ - Albumin Depletion and Low Abundance Protein Enrichment Kit
      • General Protein Compression
        • NuGel™ NRicher™ Mx (formerly NuGel PROfessor™)
      • Hemoglobin Removal/Depletion Kits
        • HemoVoid™ - Blood Card Reagent
        • HemoVoid™ - Hemoglobin Depletion From Erythrocytes
      • On-Bead Sample Prep Kit for LC-MS
        • AlbuVoid™ LC-MS On-Bead For Serum Proteomics
      • Urine Protein Enrichment for Proteomics & Biomarkers
        • UPCK™ Urine Protein Enrichment / Concentration Kit
    • Class Specific Enrichment
      • Glycoprotein Enrichment
        • NuGel™ Glycoprotein Enrichment PBA Kit - With Phenyl Boronic Acid NuGel™ Matrix
        • NuGel™ Phenyl Boronic Acid
      • Kinase (& ATP binding proteins) enrichment reagent
        • KinaSorb™ Kinase (& ATP binding proteins) Enrichment Reagent
      • Virus and Viral Component Isolation
        • Viraffinity™ - Virus and Viral Component Isolation
        • ViraPrep™ Mammal
    • Sample Prep Mass Spectrometry
      • Lipid Adsorption & Clarification Reagent
        • Cleanascite™ Lipid Removal Reagent and Clarification
      • Protein Removal for Drug Screening & Metabolomics
        • BindPro™ For Drug Binding/Screening, Metabolomics and Protein Recovery
        • BindPro™ Metabolomics - Protein Removal & Enrichment
        • BindPro™ Trial Kit
      • SDS and Non-ionic Detergent (eg. Triton) Removal
        • SurfactAway™ SDS Removal
        • SurfactAway™ Triton Removal
      • Solid-phase Protein Extraction Reagent / DNA Isolation / Microbial & Proteoglycan Enrichment
        • ProCipitate™
    • Functional & Chemical Proteomics
      • Compound Centric Chemical Proteomics (CCDP) & Protein Compression
        • NuGel™ NRicher™ Mx (formerly NuGel PROfessor™)
      • Functional Proteomics Separations and Enrichment Kit
        • NuGel™ NRicher™ 6 (formerly NuGel PROspector™)
      • Polymer Coated Silica Affinity Immobilization Matrices
        • NuGel™ Poly-Aldehyde
        • NuGel™ Poly-Amine
        • NuGel™ Poly-Carboxy
        • NuGel™ Poly-Epoxy
        • NuGel™ Poly-Hydroxy
    • Genomic Sample Prep
      • Lipid Adsorption & Clarification Reagent
        • Cleanascite™ Lipid Removal Reagent and Clarification
      • ProCipitate™-based DNA Isolation Kits
        • ProPrep™ BAC Mini Kit
        • ProPrep™ Genomic 100 Kit
      • Solid-phase Protein Extraction Reagent / DNA Isolation / Microbial & Food Safety
        • ProCipitate™
      • Virus and Viral Component Isolation
        • Viraffinity™ - Virus and Viral Component Isolation
        • ViraPrep™ Lambda
        • ViraPrep™ Mammal
    • Accessories
      • Albuvoid Buffers
        • AlbuVoid Binding Buffer AVBB™
        • AlbuVoid Buffer Kit™
        • AlbuVoid Elution Buffer AVEB™
        • AlbuVoid Wash Buffer AVWB™
      • Gift Certificate
        • Gift Certificate
      • Hemovoid Buffers
        • HemoVoid Binding Buffer HVBB™
        • HemoVoid Buffer Kit™
        • HemoVoid Elution Buffer HVEB™
        • HemoVoid Wash Buffer HVWB™
      • Tube Filter Pack
        • Corning® Spin-X Filter
      • UPCK Buffers
        • UPCK Binding Buffer UPBB™
        • UPCK Buffer Kit™
        • UPCK Elution Buffer UPEB™
        • UPCK Wash Buffer UPWB™
  • Technical Resources
    • References
    • Publications & Reports
    • FAQs
    • Case Studies
      • The Influence of Sample Prep Bias on LC-MS Targeted Peptide Quantification in Serum Proteomics
      • Re-imagining proteomics for developing precision medicine biomarkers of the innate immune response in SARS-CoV-2
      • Patent Application Describes New Proteomic Methods to Monitor Protease Inhibitor Function During Covid-19 Infections
      • Efficient Hemoglobin Removal Advances Red Cell Proteomics Offering Many New Insights Into Inflammation and Infectious Disease
      • The Potential for New Blood Biomarkers in the Management of COVID-19 Disease
      • Establishing the Utility of HemoVoid™ and HemogloBind™ as Enrichment Tools for Proteomic Analysis of Red Cells and Whole Blood in Parkinson’s Disease
      • Species Diversity Supported By BSG Products
      • Poster Report Describes Loss of Functional Serpin Activity In Cancer Patient Blood
      • AlbuVoid™️ PLUS & AlbuSorb™️ PLUS Evaluating Different Windows of Observation Solves The Many Challenges of Serum Proteomics
      • Tackling the Challenges of Serum Proteomics
      • Lipid Removal Sample Prep for Cell Response Applications
      • Sample Prep for Proteomic Analysis of Saliva
      • Biotech Support Group Featured in Book, "Functional Proteomics – Methods and Protocols"
      • Sample Prep Liquid Biopsy Products Suitable for Proteomic Profiling of a Variety of Body Fluid Sample Types
      • Albumin and High Abundance Depletion
      • Using HemogloBind™ as a Hemoglobin Binding Reagent
      • Diverse technologies available for researchers to selectively bind or enrich exosomes and extracellular vesicles.
      • Stroma Liquid Biopsy™ Biomarkers Profile Pan-Cancer Dysregulation of the Serum Proteome
      • Diverse Depletion and Enrichment Technologies Enhance Simplicity and Efficiency of Obtaining Quality Proteomic Information
      • Use On-Bead Digestion to Improve Time Required for Serum Digestion
      • Using AlbuVoid™ as a Serum Protein Enrichment Kit in Functional Proteomics
      • Using Cleanascite™ as a Lipid Absorption and Clarification Reagent
      • Using HemoVoid to Remove Hemoglobin Before Analysis
  • Contact
  • Liquid Biopsy

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800.935.0628
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Biotech Support Group
  • About
    • BSG Advantage
      • Consumable
      • Enrichment/Depletion
      • Functional Integrity
      • On-Bead Digestion
    • Business Development
    • Corporate Offices
    • News & Events
    • How to Order
  • Products
    • Hemoglobin Removal Kits
      • Hemoglobin Removal/Depletion Kits
        • HemogloBind™ Hemoglobin Deletion From Hemolyzed Serum/Plasma
        • HemoTrial™ Kit
        • HemoVoid™ - Blood Card Reagent
        • HemoVoid™ - Hemoglobin Depletion From Erythrocytes
        • HemoVoid™ - Hemoglobin Variant Enrichment From Blood
        • NuGel-HemogloBind™
      • On-Bead Sample Prep Kit for LC-MS
        • HemoVoid™ LC-MS On-Bead
    • Lipid Removal & Clarification
      • Lipid Adsorption & Clarification Reagent
        • Cleanascite™ Lipid Removal Reagent and Clarification
    • Urine Protein & Low Abundance Enrichment
      • Albumin Depletion Kits from Serum or Plasma
        • AlbuVoid™ - Albumin Depletion and Low Abundance Protein Enrichment Kit
      • General Protein Compression
        • NuGel™ NRicher™ Mx (formerly NuGel PROfessor™)
      • Hemoglobin Removal/Depletion Kits
        • HemoVoid™ - Blood Card Reagent
        • HemoVoid™ - Hemoglobin Depletion From Erythrocytes
      • On-Bead Sample Prep Kit for LC-MS
        • AlbuVoid™ LC-MS On-Bead For Serum Proteomics
      • Urine Protein Enrichment for Proteomics & Biomarkers
        • UPCK™ Urine Protein Enrichment / Concentration Kit
    • Class Specific Enrichment
      • Glycoprotein Enrichment
        • NuGel™ Glycoprotein Enrichment PBA Kit - With Phenyl Boronic Acid NuGel™ Matrix
        • NuGel™ Phenyl Boronic Acid
      • Kinase (& ATP binding proteins) enrichment reagent
        • KinaSorb™ Kinase (& ATP binding proteins) Enrichment Reagent
      • Virus and Viral Component Isolation
        • Viraffinity™ - Virus and Viral Component Isolation
        • ViraPrep™ Mammal
    • Sample Prep Mass Spectrometry
      • Lipid Adsorption & Clarification Reagent
        • Cleanascite™ Lipid Removal Reagent and Clarification
      • Protein Removal for Drug Screening & Metabolomics
        • BindPro™ For Drug Binding/Screening, Metabolomics and Protein Recovery
        • BindPro™ Metabolomics - Protein Removal & Enrichment
        • BindPro™ Trial Kit
      • SDS and Non-ionic Detergent (eg. Triton) Removal
        • SurfactAway™ SDS Removal
        • SurfactAway™ Triton Removal
      • Solid-phase Protein Extraction Reagent / DNA Isolation / Microbial & Proteoglycan Enrichment
        • ProCipitate™
    • Functional & Chemical Proteomics
      • Compound Centric Chemical Proteomics (CCDP) & Protein Compression
        • NuGel™ NRicher™ Mx (formerly NuGel PROfessor™)
      • Functional Proteomics Separations and Enrichment Kit
        • NuGel™ NRicher™ 6 (formerly NuGel PROspector™)
      • Polymer Coated Silica Affinity Immobilization Matrices
        • NuGel™ Poly-Aldehyde
        • NuGel™ Poly-Amine
        • NuGel™ Poly-Carboxy
        • NuGel™ Poly-Epoxy
        • NuGel™ Poly-Hydroxy
    • Genomic Sample Prep
      • Lipid Adsorption & Clarification Reagent
        • Cleanascite™ Lipid Removal Reagent and Clarification
      • ProCipitate™-based DNA Isolation Kits
        • ProPrep™ BAC Mini Kit
        • ProPrep™ Genomic 100 Kit
      • Solid-phase Protein Extraction Reagent / DNA Isolation / Microbial & Food Safety
        • ProCipitate™
      • Virus and Viral Component Isolation
        • Viraffinity™ - Virus and Viral Component Isolation
        • ViraPrep™ Lambda
        • ViraPrep™ Mammal
    • Accessories
      • Albuvoid Buffers
        • AlbuVoid Binding Buffer AVBB™
        • AlbuVoid Buffer Kit™
        • AlbuVoid Elution Buffer AVEB™
        • AlbuVoid Wash Buffer AVWB™
      • Gift Certificate
        • Gift Certificate
      • Hemovoid Buffers
        • HemoVoid Binding Buffer HVBB™
        • HemoVoid Buffer Kit™
        • HemoVoid Elution Buffer HVEB™
        • HemoVoid Wash Buffer HVWB™
      • Tube Filter Pack
        • Corning® Spin-X Filter
      • UPCK Buffers
        • UPCK Binding Buffer UPBB™
        • UPCK Buffer Kit™
        • UPCK Elution Buffer UPEB™
        • UPCK Wash Buffer UPWB™
  • Technical Resources
    • References
    • Publications & Reports
    • FAQs
    • Case Studies
      • The Influence of Sample Prep Bias on LC-MS Targeted Peptide Quantification in Serum Proteomics
      • Re-imagining proteomics for developing precision medicine biomarkers of the innate immune response in SARS-CoV-2
      • Patent Application Describes New Proteomic Methods to Monitor Protease Inhibitor Function During Covid-19 Infections
      • Efficient Hemoglobin Removal Advances Red Cell Proteomics Offering Many New Insights Into Inflammation and Infectious Disease
      • The Potential for New Blood Biomarkers in the Management of COVID-19 Disease
      • Establishing the Utility of HemoVoid™ and HemogloBind™ as Enrichment Tools for Proteomic Analysis of Red Cells and Whole Blood in Parkinson’s Disease
      • Species Diversity Supported By BSG Products
      • Poster Report Describes Loss of Functional Serpin Activity In Cancer Patient Blood
      • AlbuVoid™️ PLUS & AlbuSorb™️ PLUS Evaluating Different Windows of Observation Solves The Many Challenges of Serum Proteomics
      • Tackling the Challenges of Serum Proteomics
      • Lipid Removal Sample Prep for Cell Response Applications
      • Sample Prep for Proteomic Analysis of Saliva
      • Biotech Support Group Featured in Book, "Functional Proteomics – Methods and Protocols"
      • Sample Prep Liquid Biopsy Products Suitable for Proteomic Profiling of a Variety of Body Fluid Sample Types
      • Albumin and High Abundance Depletion
      • Using HemogloBind™ as a Hemoglobin Binding Reagent
      • Diverse technologies available for researchers to selectively bind or enrich exosomes and extracellular vesicles.
      • Stroma Liquid Biopsy™ Biomarkers Profile Pan-Cancer Dysregulation of the Serum Proteome
      • Diverse Depletion and Enrichment Technologies Enhance Simplicity and Efficiency of Obtaining Quality Proteomic Information
      • Use On-Bead Digestion to Improve Time Required for Serum Digestion
      • Using AlbuVoid™ as a Serum Protein Enrichment Kit in Functional Proteomics
      • Using Cleanascite™ as a Lipid Absorption and Clarification Reagent
      • Using HemoVoid to Remove Hemoglobin Before Analysis
  • Contact
  • Liquid Biopsy
Pandemic Research Products that may assist in COVID-19 and ARDS research Hemoglobin Removal The GOLD Standard Products Why Waste Time and Money Using Antibodies for Depletion Biotech Support Group helps enrich your proteome better. Lipid Removal & Clarification for the Most Challenging Applications ProCipitate for DNA/RNA Isolation Superior Substitute to Phenol-Chloroform Removes all interference from Hemoglobin & Proteins Raise Your Research Our products have been adapted to all diseases and analytical platforms. The BSG Advantage Consumable, Enrichment/Depletion, On-Bead Digestion, & Functional Integrity


Research Article Cites NuGel™ PBA for Proteome Analysis of Saliva


Research Article Cites HemoVoid™ in Fish Red Blood Cell Proteome Study


Biotech Support Group Presents Stroma Liquid Biopsy Cancer Data at HUPO World Congress 2017

Research Article Cites HemogloBind™ In Diabetic Complications Study


Research Article Cites HemoVoid™ in Functional Proteome Study


Research Article Cites Cleanascite™ for Analyzing Membrane Interaction Proteins


Journal Article Highlights AlbuVoid™ in Cancer Functional Proteomic Analysis


Research Article Cites HemogloBind™ in Parkinson’s Disease Study



BSG•OMx Services

  • Functional Proteomics
  • Chemical Proteomics [CCDP™]
  • Serum Proteomics LC-MS

BSG Advantage

  • Consumable
  • Enrichment/Depletion
  • Functional Integrity
  • On-Bead Digestion

Raise Your Research

Our Case Studies describe how the BSG Advantages have been adapted to all diseases and analytical platforms

  • Learn More

Raise Your Research

Click on the Case Studies below to learn more about how the BSG Consumable Advantage has been successfully employed

  • Using HemogloBind™ as a Hemoglobin Binding Reagent
  • Diverse technologies available for researchers to selectively bind or enrich exosomes and extracellular vesicles
  • Tackling the Challenges of Serum Proteomics - AlbuVoid™ PLUS - Albumin & IgG Depletion

Raise Your Research

Click on the Case Studies below to learn more about how the BSG Enrichment/Depletion Advantage has been successfully employed

  • Sample Prep Liquid Biopsy Products Suitable for Proteomic Profiling of a Variety of Body Fluid Sample Types
  • Albumin Depletion and Enrichment Technologies Enhance Efficiency and Simplicity of Achieving Quality Proteomic Information
  • Hemoglobin Depletion and Enrichment Technologies Enhance Simplicity and Efficiency of Obtaining Quality Proteomic Information

Raise Your Research

Click on the Case Studies below to learn more about how the BSG Functional Integrity Advantage has been successfully employed

  • Lipid Removal Sample Prep for Cell Response Applications
  • Using AlbuVoid™ as a Serum Protein Enrichment Kit in Functional Proteomics
  • Using HemoVoid™ to Remove Hemoglobin Before Analysis

Raise Your Research

Click on the Case Studies below to learn more about how the BSG On-Bead Digestion Advantage has been successfully employed

  • Tackling the Challenges of Serum Proteomics - AlbuVoid™ PLUS - Albumin & IgG Depletion
  • Use On-Bead Digestion to Improve Time Required for Serum Digestion

Proteomic & Genomic Sample Prep and Enrichment

Albumin & IgG Removal Kits

  • Unique surface chemistries
  • depletes Albumin 90-95%
  • Species agnostic
  • Retains function and bio-activity
Learn more

Hemoglobin Removal Kits

  • Unique surface chemistries
  • depletes Hemoglobin 90-95%
  • Species agnostic
  • Retains function and bio-activity
Learn more

Lipid Removal & Clarification

  • Extensively cited
  • Replaces hazardous hydrocarbons
  • Diverse samples
  •  
Learn more
View All Products >>

News & Events

September 9, 2021
Two Journal Articles Cite Cleanascite™ in Studies of Lipid Influences in Cell Response Assays

September 8, 2021
Two journal articles cite HemogloBind™ to remove analytical interferences from Hemoglobin

July 26, 2021
Journal Article Highlights AlbuVoid™ in Cell Secretome Proteome Analysis

July 22, 2021
HemogloBind™ Cited in Forensics Multiplex Proteomic Assay

May 20, 2021
Leiden University and Biotech Support Group Report Progress on Stromal Conditioning Biomarkers in Cancer

April 15, 2021
Journal Article Cites HemoVoid™ and AlbuVoid™ Enrichment for LC-MS Proteomic Analysis of Osteogenic and Fibrogenic Blood Composites

March 19, 2021
Journal Article Cites AlbuSorb™ for Analysis of High-fat Diet–induced Metabolic Changes

February 20, 2021
Cleanascite™ Employed to Investigate Central Nervous System (CNS) Cell Response

February 16, 2021
HemogloBind™ Cited in Comparison Proteomics Study of Covid-19 Patients in Peripheral Blood – The Barometer of the Immune System

January 23, 2021
HemogloBind™ Cited in Pesticide Toxicology Study of Post-Mortem Changes in Blood

January 15, 2021
Patent Application Describes HemoVoid™ To Uncover Proteomic Changes from Autologous Blood-doping

December 3, 2020
Research Article Cites HemoVoid™ in Biomarker Study for Obstructive Sleep Apnea

December 3, 2020
Review Article Cites Cleanascite™ To Analyze Lipid-based Nanocarriers

October 30, 2020
Patent Application Describes New Proteomic Methods to Monitor Protease Inhibitor Function During Covid-19 Infections

October 7, 2020
Research Article Cites Cleanascite™ In Lipid Factor Determination of Immune Response in Plants

October 7, 2020
Cleanascite™ Cited in the Preparation of Monoclonal Antibodies

September 18, 2020
Malaria research article cites use of HemogloBind™ in reticulocyte study

September 3, 2020
Journal Article Cites Cleanascite™ in Study of a Traditional Chinese Medicine As Anticancer Agent

August 27, 2020
Nature Article Cites Cleanascite™ to Investigate Serum Lipid Factors Linking Ageing and Cancer Progression

View Our References

Case Studies

The Influence of Sample Prep Bias on LC-MS Targeted Peptide Quantification in Serum Proteomics

With advancements in LC-MS instrumentation, proteomic productivity is moving from protein identifications and Venn diagrams, to establishing quantitative differences between proteins in samples representing a challenge or disease state, vs. samples representing a normal or control state. Peptides of interest are usually selected from discovery investigations and can serve as proxies for either total protein or functional amounts.

With the introduction of targeted analysis, sensitivity and technical variance in LC-MS/MS analysis has dramatically improved. Nevertheless, for serum/plasma samples, small biological variances remain hard to measure robustly. So validating small differences from discovery must translate to targeted quantification and that has become the main focus of BSG’s enrichment/depletion product line. In this case study we show how sample prep does matter. By not relying on immuno-affinity for depletion, BSG has strived to address and service the unique demands that come with validating targeted peptide analysis, to accelerate proteomics from discovery to the clinic.

Read More >>
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