News Release

Cleanascite™ Cited In Study of Lipid Biology in Endoplasmic Reticulum Stress

MONMOUTH JUNCTION, NJ, April 2, 2025 -- Biotech Support Group reports on an article, describing the simplicity and efficiency of their lipid clearance product to study universal lipid factors associated with endoplasmic reticulum stress and metabolic diseases.

The citation is:

Huo, Yazhen, et al. "Ceramide mediates cell-to-cell ER stress transmission by modulating membrane fluidity." Journal of Cell Biology 224.5 (2025): e202405060.

Under endoplasmic reticulum (ER) stress (ERS), cells initiate the unfolded protein response (UPR) to maintain ER homeostasis. Recent studies revealed ERS transmission between cells and tissues, by activating the cell-nonautonomous UPR in cells that do not experience ERS directly. Here, we report that ERS triggers a rapid release of ceramide independent of the UPR, but requiring the acid sphingomyelinase activity. Carried by lipoproteins, ceramide is delivered to receiving cells to induce the UPR and regulate cell functions at multiple aspects, including lipid accumulation, cell death, and cytokine production. 

The article states “We next tried to characterize the signaling molecules in Conditioned Media (CM) that elicited cell-nonautonomous UPR. … Interestingly, treatment with a lipid removal reagent Cleanascite eliminated the effect of CM in inducing Xbp1 splicing (Fig. 2B). … The above results pin-pointed lipids as the universal factor to relay the ERS signals between cells.”

The study suggests ceramide-mediated ERS transmission as a universal cell–cell communication model regulating a wide range of physiological events, and might in part contribute to metabolic diseases, including diabetes, obesity, and fatty liver disease. 

“This is yet another example of how Cleanascite™ was instrumental in determining whether lipids and associated factors were the critical signaling element in the cellular response system. We now have over thirty such references. Unlike alternative lipid-depletion methods that use solvents, Cleanascite™ is an aqueous suspension product and so it is very compatible with cellular models of disease. As shown in this reference, the essential requirement is the maintenance of cellular activity after lipid depletion, without this feature, these types of investigations would not be possible.” states Swapan Roy, Ph.D., President and Founder of Biotech Support Group.

For more information visit: Cleanascite™ Lipid Removal Reagent and Clarification, at
http://www.biotechsupportgroup.com/Cleanascite-Lipid-Removal-Reagent-p/x2555.htm

Keywords: endoplasmic reticulum (ER), endoplasmic reticulum stress (ERS), unfolded protein response (UPR), ceramide, lipid biology, diabetes, obesity, fatty liver disease, Cleanascite™, lipid depletion

About Biotech Support Group LLC

Converging with cultural and technological disruptions forthcoming in healthcare, Biotech Support Group develops methods for cost effective and efficient sample prep essential for expanding proteomic analysis. Following a tiered business strategy, the company continues its growth in the consumable research products area. For this market, key products include: AlbuVoid™ and AlbuSorb™ PLUS for albumin & IgG depletion, Cleanascite™ for lipid adsorption, HemogloBind™ and HemoVoid™ for hemoglobin removal, and NRicher™ for low abundance, family specific, and targeted proteome enrichment. For more information, go to http://www.biotechsupportgroup.com

For Business Development, contact: Matthew Kuruc 732-274-2866, mkuruc@biotechsupportgroup.com