ViraPrep™ Lambda

Product Code: VLK

ViraPrep™ Lambda

Complete DNA purification kit includes Viraffinity™, conditioning buffer, RNase and lysis buffer.
Extremely rapid and simple-to-use. The entire protocol is complete in less than 1.5 hours
Phage coat proteins and exonuclease remain bound to the polymer surface
Non-hazardous, no polyethylene glycol

Click Here For ViraPrep™ Lambda Product Sheet

Product	size	Item No.
ViraPrep™ Lambda 05	5, 150 mm plates	VLK-05

Human mouth virus

Yu, H. T., Jiang, H., Zhang, Y., Nan, X. P., Li, Y., Wang, W., ... & Wang, P. Z. (2012). Hantaan virus triggers TLR4-dependent innate immune responses. Viral immunology, 25(5), 387-393.

Virus particles

Al-Jarbou, A. (2009). Metagenomic Analysis of the Human Mouth Virus Population and Characterisation of Two Lytic Viruses (Doctoral dissertation, University of Leicester).

Do BSG's products work for all species?

Yes, as our products are not derived from antibodies, they are species agnostic, and have applied to humans, mice, rats, guinea pigs, goats, cows, pigs, even frogs and fish.

Can you supply bulk material only (from the kit-based products) for large volume orders?

Yes, we can adjust all of our kit products, to supply only the beads. For large orders, whether kit or bulk material, we provide special discounted quotations. Please inquire.

Can the protocol be scaled to accommodate different amounts of either volume or mass?

Yes, we provide general guidelines for use, but the protocols can usually be adjusted to accommodate either larger or small starting samples, or larger or smaller starting concentrations. We recommend first contacting us for guidance in adjusting the protocol.

Can the products be adapted to high throughput formats and automation?

Although we do not have an off-the-shelf product for 96-well formats currently, we have ongoing collaborations that can provide such formats. Please inquire.

Are the products compatible with detergents?

This has not been fully investigated, but we do not recommend use of SDS. Low concentrations, 0.1 – 0.5% of non-ionic detergents have been used with some of our products, but to what extent they alter separation performance, is unknown. If non-ionic detergents are to be used, generally we recommend that they be of no greater than 0.1% before applying to the beads. So for tissue lysates for example at higher %, dilute the sample before application.

What is the stability and shelf life of the product?

The products have a shelf life typically 6 months to 1 year. We ship at room temperature, but recommend storage at 4°C upon receipt. Do not freeze.

Do the products contain any biologically derived material?

All of our products are derived chemically, not biologically, with one exception. The "PLUS" products contain recombinant Protein A.

How can I order?

We can take on-line cart orders, which accept North American credit cards, Visa & MasterCard. It does not however, accept other international credit cards. If you would like to order direct, contact us at [email protected]. In the US, distributors Fisher Scientific and VWR can be used to order all products, even those not listed on their sites, just provide them with our catalog #. A full list of international distributors is listed on How to Order | Biotech Support Group LLC.

Kit includes:

Items Required	Quantity	ViraPrep™Lambda	Storage
LL1, Buffer (for liquid lysates)	500 ul	Supplied	4°C
HS1, Solubilization Buffer (for plate lysates)	150 ml	Supplied	4°C
RNase Cocktail	1 ml	Supplied	-20°C
HL2, Lysis Buffer	10 ml	Supplied	4°C
AA3, Ammonium Acetate	1 ml	Supplied	4°C
V1062, Viraffinity™	10 ml	Supplied	4°C
Ethanol	Not Supplied	-
Growth Media	Not Supplied	-
Final Resuspension Buffer	Not Supplied	-

Culture Conditions	Titer	DNA Yield	% Bound
150mm plate lysate, solubilized in 10ml ViraPrep™ Lambda HS1 buffer, and clarified	10 ⁹ pfu/ml (approximate)	10 - 20 ”�g	>95
10ml liquid lysate, plus addition of LL1 buffer, and clarified	10 ⁹ pfu/ml (approximate)	10 - 20 ”�g	>95

MSDS:
MSDS-ViraPrep™ Lambda AA3 Buffer
MSDS-ViraPrep™ Lambda Lysis Buffer HL2
MSDS-ViraPrep™ Lambda HS1 Binding Buffer
MSDS-ViraPrep™ Lambda HL2 Lysis Binding Buffer
MSDS-ViraPrep™ Lambda Binding Buffer LL1

Product Code	Name	Base Price
Product Code:VLK-05	ViraPrep Lambda -150 mm plate lysates (Qty 5), or 10 mL liquid lysates (Qty 25)	Base Price:$515.00
Product Code:VLK-0001	ViraPrep Lambda - VLK-25, 10 mL liquid lysates (Qty 25)	Base Price:$355.00
Product Code:VLK-25	ViraPrep Lambda	Base Price:$355.00

ViraPrep™ Lambda

Description

References

FAQ