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References

Albumin Depletion

AlbuSorb™ Albumin Depletion Kit

Cerebrospinal Fluid


Gwenael Pottiez, Pawel Ciborowski. Proteomic Profiling of Cerebrospinal Fluid Expression Profiling In Neuroscience Neuromethods.2012;64:245-270


Synovial fluid


Happonen KE, Fürst CM, Saxne T et al. PRELP protein inhibits the formation of the complement membrane attack complex . Journal of Biological Chemistry.2012;287(11):8092-100


Serum


Berggren, Per-olof, and Lisa Juntti-berggren. "Methods for treating and/or limiting development of diabetes." U.S. Patent No. 20,170,037,118. 9 Feb. 2017 .MOJ Proteomics Bioinform 2016, 3(6): 00106


Swapan Roy, Matthew Kuruc. The Functional Subproteomes of Serpin Protease Inhibitors are Now Open for LC-MS Biomarker Discovery .MOJ Proteomics Bioinform 2016, 3(6): 00106


Holmberg R, Refai E, Höög A. Lowering apolipoprotein CIII delays onset of type 1 diabetes . Proceedings of the National Academy of Sciences.2011;108(26):10685-9.


Tang MX, Ogawa K, Asamoto M. Effects of Nobiletin on PhIP-Induced Prostate and Colon Carcinogenesis in F344 Rats Nutrition and Cancer.2011;63(2):227-33


Holmberg, Rebecka Apolipoprotein CIII and Ljungan virus in diabetes 2010. Doctoral Thesis


Lu Q, Zheng X, McIntosh T Development of different analysis platforms with LC-MS for pharmacokinetic studies of protein drugs . Analytical Chemistry.2009;81(21):8715-23


Urine


Zubiri, Irene, et al. " Diabetic nephropathy induces changes in the proteome of human urinary exosomes as revealed by label-free comparative analysis ." Journal of proteomics 96 (2014): 92-102.


Patent


Berggren, Per Olaf, Yang, Shao-Nian. 2012. Methods For Treating And/Or Limiting Development Of Diabetes . U.S. Patent 20120328630 Kind Code: A1, filed June 25, 2012, and issued December 27, 2012.


AlbuTrial™ Kit - AlbuVoid™ and AlbuSorb™

Cerebrospinal Fluid

Gwenael Pottiez, Pawel Ciborowski. Proteomic Profiling of Cerebrospinal Fluid Expression Profiling In Neuroscience Neuromethods.2012;64:245-270


Synovial fluid


Happonen KE, Fürst CM, Saxne T et al. PRELP protein inhibits the formation of the complement membrane attack complex . Journal of Biological Chemistry.2012;287(11):8092-100


Serum


Holmberg R, Refai E, Höög A. Lowering apolipoprotein CIII delays onset of type 1 diabetes. Proceedings of the National Academy of Sciences.2011;108(26):10685-9.


Tang MX, Ogawa K, Asamoto M. Effects of Nobiletin on PhIP-Induced Prostate and Colon Carcinogenesis in F344 Rats Nutrition and Cancer.2011;63(2):227-33


Holmberg, Rebecka Apolipoprotein CIII and Ljungan virus in diabetes 2010. Doctoral Thesis


Lu Q, Zheng X, McIntosh T Development of different analysis platforms with LC-MS for pharmacokinetic studies of protein drugs . Analytical Chemistry.2009;81(21):8715-23


AlbuVoid™ References

Application Report


Haiyan Zheng 1; Swapan Roy 2; Devjit Roy MD 2; Amenah Soherwardy 1; Seema Rathman 2; Matthew Kuruc 2 1Rutgers Center for Integrative Proteomics, Piscataway, NJ; 2Biotech Support Group LLC, Monmouth Junction, NJ Stroma Liquid Biopsy – Pan-Cancer Dysregulation of the Serum Proteome


Serum


David L. Wang, Chuanguang Xiao, Guofeng Fu, Xing Wang and Liang Li. " Identification of potential serum biomarkers for breast cancer using a functional proteomics technology. ." Biomarker Research (2017) 5:11.. DOI 10.1186/s40364-017-0092-9


Sun, Zhenyu, Xiaofeng Chen, Gan Wang, Liang Li, Guofeng Fu, Matthew Kuruc, and Xing Wang. " Identification of functional metabolic biomarkers from lung cancer patient serum using PEP technology ." Biomarker Research 4, no. 1 (2016): 1.


Haiyan Zheng; Caifeng Zhao; Swapan Roy; Devjit Roy MD; Amenah Soherwardy; Ravish Amin; Matthew Kuruc. Application Report - The Comparison of the Serum Proteome in Individuals with Cancers versus those without Cancer, and its application to Wellness


Dr.Swapan Roy; Amenah Soherwardy; Ravish Amin; Matthew Kuruc.Monmouth Junction, NJ - Application Report. AlbuSorb™ Product Extension combines Albumin and Immunoglobulin Depletion in a Consumable Format


Xing Wang, Michael Davies, Swapan Roy and Matthew Kuruc. Bead Based Proteome Enrichment Enhances Features of the Protein Elution Plate (PEP) for Functional Proteomic Profiling . Proteomes 2015, 3(4), 454-466. doi: 10.3390/proteomes3040454


Grubbs, J. K., et al. " Investigation of the efficacy of albumin removal procedures on porcine serum proteome profile1 ." (2015).


Discovery of Functional Serum Biomarkers Using AlbuVoid™ Enrichment and the ArrayBridge PEP Profiling Platform . Personal communication, Xing Wang, Ph.D., ArrayBridge (St. Louis, MO), manuscripts in process.


Serum Profiling Making Mark on Predictive Medicine

Vicki Glaser.Genetic Engineering & Biotechnology News. 2011;31(7):1-55.


Kuruc Matt. Application Report - AlbuVoid™ & On-Bead Digestion - Tackling the challenges of serum proteomics (LC-MS).


Kuruc Matt, Dr.Roy. Application Report - AlbuVoid™ LC-MS On-Bead – Differential Expression of Cancer Sera Proteins Using Quantitative (iTRAQ) Proteomics


Patents


Narain, Niven Rajin, Rangaprasad Sarangarajan, and Vivek K. Vishnudas. " INTERROGATORY CELL-BASED ASSAYS AND USES THEREOF ." U.S. Patent No. 20,120,258,874. 11 Oct. 2012.


Plasma


Espes, Daniel, Joey Lau, and Per-Ola Carlsson. " Increased circulating levels of betatrophin in individuals with long-standing type 1 diabetes ." Diabetologia(2013): 1-4.


Patent - Secretome Sample Preparation


Narain, Niven Rajin, and Paula Patricia Narain. " COMPOSITIONS AND METHODS FOR DIAGNOSIS AND TREATMENT OF PERVASIVE DEVELOPMENTAL DISORDER ." U.S. Patent No. 20,150,023,949. 22 Jan. 2015.


Narain, Niven Rajin, Rangaprasad Sarangarajan, and Vivek K. Vishnudas. " INTERROGATORY CELL-BASED ASSAYS AND USES THEREOF ." U.S. Patent No. 20,120,258,874. 11 Oct. 2012.


Cell Culture


Narain, Niven Rajin, Rangaprasad Sarangarajan, Vivek K. Vishnudas, and Michael Andrew Kiebish. " USE OF MARKERS IN THE IDENTIFICATION OF CARDIOTOXIC AGENTS AND IN THE DIAGNOSIS AND MONITORING OF CARDIOMYOPATHY AND CARDIOVASCULAR DISEASE ." U.S. Patent 20,140,100,128, issued April 10, 2014.


On-Bead Digestion Protocols For LC-MS Proteomic Workflows

New on-bead digestion for LC-MS applications for proteomic studies

US HUPO 2014. Frontiers in Proteomics: Advancing Biology through Technology and Computation.


AlbuVoid™ poster, entitled " Improved proteomic enrichment and workflow strategies ", poster board 089 presented at US HUPO 2014


References for AlbuSorb™ PLUS

Application Report


AlbuSorb™ Product Extension combines Albumin and Immunoglobulin Depletion in a Consumable Format

Hemoglobin Depletion

HemogloBind™ - Hemoglobin Depletion From Hemolyzed Serum/Plasma

Bronchoalveolar lavage fluid (BALF)

Maneesh Bhargava, Kevin J. Viken, Sanjoy Dey, Michael S. Steinbach, Baolin Wu, Pratik D. Jagtap, LeeAnn Higgins, Angela Panoskaltsis-Mortari, Daniel J. Weisdorf, Vipin Kumar, Mukta Arora, Peter B. Bitterman, David H. Ingbar, Chris H. Wendt.
Proteome Profiling in Lung Injury after Hematopoietic Stem Cell Transplantation . Biology of Blood and Marrow Transplantation 22 (2016) 1383-1390.

Cell Lines

Phanse, Sadhna, Cuihong Wan, Blake Borgeson, Fan Tu, Kevin Drew, Greg Clark, Xuejian Xiong et al. " Proteome-wide dataset supporting the study of ancient metazoan macromolecular complexes ." Data in brief 6 (2016): 715-721.

Dried Blood Spot(DBS)/Whole Blood

Hakuna, Lovemore, et al. " A simple assay for glutathione in whole blood ."Analyst (2015).

Whole Blood

Chalásová, Katarína, et al. "Transketolase Activity but not Thiamine Membrane Transport Change in Response to Hyperglycaemia and Kidney Dysfunction ." Experimental and Clinical Endocrinology & Diabetes (2017).

Chalásová, Katarína.
Functional analysis of genetic variants in genes associated with the development of glycotoxic damage . Diss. Masarykova univerzita, Lékařská fakulta, 2017.

Lahut, Suna, et al. " Blood RNA biomarkers in prodromal PARK4 and REM sleep behavior disorder show role of complexin-1 loss for risk of Parkinson's disease. " Disease Models & Mechanisms (2017): dmm-028035.

Brittain, Matthew K., Kevin G. McGarry, Robert A. Moyer, Michael C. Babin, David A. Jett, Gennady E. Platoff, and David T. Yeung. " Efficacy of Recommended Prehospital Human Equivalent Doses of Atropine and Pralidoxime Against the Toxic Effects of Carbamate Poisoning in the Hartley Guinea Pig ." International journal of toxicology (2016): 1091581816638086.

Snider, Thomas H., Kevin G. McGarry, Michael C. Babin, David A. Jett, Gennady E. Platoff Jr, and David T. Yeung. "
Acute toxicity of phorate oxon by oral gavage in the Sprague-Dawley rat ." Fundamental Toxicological Sciences 3, no. 5 (2016): 195-204.

Bhargava, Maneesh, Kevin J. Viken, Sanjoy Dey, Michael S. Steinbach, Baolin Wu, Pratik D. Jagtap, LeeAnn Higgins et al. " Proteome Profiling in Lung Injury after Hematopoietic Stem Cell Transplantation ." Biology of Blood and Marrow Transplantation (2016).

Snider, Thomas H., Christina M. Wilhelm, Michael C. Babin, Gennady E. Platoff Jr, and David T. Yeung. "
Assessing the therapeutic efficacy of oxime therapies against percutaneous organophosphorus pesticide and nerve agent challenges in the Hartley guinea pig ."The Journal of Toxicological Sciences 40, no. 6 (2015): 759-775.

Blood Plasma

Johns, Michael, et al. "SR-135, a peroxynitrite decomposing catalyst, enhances β-cell function and survival in B6D2F1 mice fed a high fat diet. "Archives of Biochemistry and Biophysics (2015).

Asleh, Rabea, et al. " Haptoglobin Genotype-dependent Differences in Macrophage Lysosomal Oxidative Injury ." Journal of Biological Chemistry 289.23 (2014): 16313-16325.

Biological Fluids
J Krupey - United States Patent: 10/180,053, 2002 Removal of extraneous substances from biological fluids containing nucleic acids and the recovery of nucleic acids

Lung Tissue Specimens & Pulmonary Research (Acute Respiratory Distress Syndrome & Acute Hypoxic Failure)
Bhargava, Maneesh" "Proteomic Studies in Acute Hypoxic Respiratory Failure." PhD diss., UNIVERSITY OF MINNESOTA . (2015).

Red Blood Cell (RBC) - Lysates.
Kyoungsook Park, Christopher D. Saudek, and Gerald W. Hart Increased Expression of β-N-Acetylglucosamindase (O-GlcNAcase) in Erythrocytes from Prediabetic and Diabetic Individuals. Diabetes.2010;59(7):1845-50.

Barasa, Benjamin, and Monique Slijper. " Challenges for red blood cell biomarker discovery through proteomics ." Biochimica et Biophysica Acta (BBA)-Proteins and Proteomics 1844.5 (2014): 1003-1010

Stored Blood Products
Delobel J., Rubin O., Prudent M., Crettaz D., Tissot J.-D., Lion N.(2010) Biomarker Analysis of Stored Blood Products: Emphasis on Pre-Analytical Issues . International Journal of Molecular Sciences. 11(11):4601-4617

Red Blood Cells/Forensic Research
Danielson, Phillip B. " Isolation of Highly Specific Protein Markers for the Identification of Biological Stains: Adapting Comparative Proteomics to Forensics. " (2011).

Red Blood Cells (RBC) - Blood.
Wilhelm, Christina M., et al. A comprehensive evaluation of the efficacy of leading oxime therapies in guinea pigs exposed to organophosphorus chemical warfare agents or pesticides. Toxicology and Applied Pharmacology Available online 31 October 2014. doi:10.1016/j.taap.2014.10.009

Hikosaka, Keisuke, et al. "
Deficiency of Nicotinamide Mononucleotide Adenylyltransferase 3 (Nmnat3) Causes Hemolytic Anemia by Altering the Glycolytic Flow in Mature Erythrocyte" Journal of Biological Chemistry (2014): jbc-M114.

McGarry, Kevin G., et al. " Evaluation of HemogloBind™ treatment for preparation of samples for cholinesterase analysis ." (2013). Advances in Bioscience and Biotechnology, 2013, 4, 1020-1023

Alvarez-Llamas, Gloria, Fernando de la Cuesta, Maria G. Barderas, Irene Zubiri, Maria Posada-Ayala, and Fernando Vivanco. " Characterization of Membrane and Cytosolic Proteins of Erythrocytes. " In Vascular Proteomics, pp. 71-80. Humana Press, 2013.

Alvarez-Llamas, G., de la Cuesta, F., Barderas, M. G., Darde, V. M., Zubiri, I., Caramelo, C., Vivanco, F. A novel methodology for the analysis of membrane and cytosolic sub-proteomes of erythrocytes by 2-DE. Electrophoresis.2009;30:4095-4108

Zihao Wang, Kyoungsook Park, Frank Comer1, Linda C. Hsieh-Wilson, Christopher D. Saudek, Gerald W. Hart. Site-Specific GlcNAcylation of Human Erythrocyte Proteins: Potential Biomarker(s) for Diabetes Mellitus . Diabetes.2008;58, 309-317.

Datta, Pradip. Effect of Hemolysis, High Bilirubin, Lipemia, Paraproteins, and System Factors on Therapeutic Drug Monitoring . Handbook of Drug Monitoring Methods.2008; 97-109.

Yuichi Miki, Tomoki Tazawa, Kazuya Hirano, Hideki Matsushima, Shoko Kumamoto, Naotaka Hamasaki, Tomohiro Yamaguchi, Masatoshi Beppu. Clearance of oxidized erythrocytes by macrophages: Involvement of caspases in the generation of clearance signal at band 3 glycoprotein . Biochemical and Biophysical Research Communications.2007; 363(1):57-62

Sarawathi,et al., Relative quantification of glycated Cu-Zn superoxide dismutase in erythrocytes by electrospray ionization mass spectrometry , Biochimica et Biophysica Acta. 1999.1426(3):483-90

Bilirubin
Krishna, Neel K., and Kenji Cunnion. " Derivative Peptide Compounds and Methods of Use ." U.S. Patent No. 20,160,376,322. 29 Dec. 2016.

Person, N.B., Effect Of HemogloBind™ On Interference Reduction In Bilirubin Analysis.poster Clinichem, 1995.

Serum
Nishida-Aoki, Nao, et al. " Disruption of Circulating Extracellular Vesicles as a Novel Therapeutic Strategy against Cancer Metastasis ." Molecular Therapy 25.1 (2017): 181-191.

Baion, C.M. & Ali, A.C.
Evaluation Of HemogloBind™ For Removal Of O-Raffinose Crosslinked Hemoglobin (Hemolink™) From Serum , poster AACC Meeting 1997.

Tissue
Padilla, S., Convenient Method for Decreasing the Amount of Hemoglobin in Tissue Samples Without Affecting the Level of Cholinesterase Activity, unpublished personal correspondence, 1994.

HemoVoid™ References

Rainbow Trout Fish Nucleated Red Blood Cells (RBCs)
Puente-Marin, Sara, Iván Nombela, Sergio Ciordia, María Carmen Mena, Verónica Chico, Julio Coll, and María del Mar Ortega-Villaizan. "
In Silico Functional Networks Identified in Fish Nucleated Red Blood Cells by Means of Transcriptomic and Proteomic Profiling ." Genes 9, no. 4 (2018): 202.

Nombela I, Puente-Marin S, Chico V et al." "Identification of diverse defense mechanisms in trout red blood cells in response to VHSV halted viral replication [version 1; referees: awaiting peer review]." ." F1000Research 2017, 6:1958 (doi: 10.12688/f1000research.12985.1)

Nombela, Ivan, Sara Puente-Marin, Veronica Chico, Alberto J. Villena, Begoña Carracedo, Sergio Ciordia, Maria Carmen Mena et al. " trout red blood cells in response to halted replication of VHS virus [version 2; referees: 3 approved with reservations]."

Rogers, Stephen C., and Allan Doctor. " Hypoxia modulates the purine salvage pathway and decreases red blood cell and supernatant levels of hypoxanthine during refrigerated storage ." (2018).


Cortese-Krott, Miriam M., et al."
"Identification of a soluble guanylate cyclase in RBCs: preserved activity in patients with coronary artery disease." ." Redox Biology (2017).

Carvalho, Ana Sofia, Manuel S. Rodriguez, and Rune Matthiesen. " Red Blood Cells in Clinical Proteomics ." Serum/Plasma Proteomics: Methods and Protocols (2017): 173-181.

Human Red Blood Cells (RBC)

Nemkov, Travis, Kaiqi Sun, Julie A. Reisz, Anren Song, Tatsuro Yoshida, Andrew Dunham, Matthew J. Wither et al. " Hypoxia modulates the purine salvage pathway and decreases red blood cell and supernatant levels of hypoxanthine during refrigerated storage ." haematologica 103, no. 2 (2018): 361-372.


Feliciano, Amélia, Fátima Vaz, Cristina Valentim-Coelho, Vukosava M. Torres, Rita Silva, Vesna Prosinecki, Bruno M. Alexandre et al. "
Evening and morning alterations in Obstructive Sleep Apnea red blood cell proteome ." Data in Brief (2017).

HemoVoid™ On Bead Digestion Application Work On RBC by Irene Granlund, Umeå University

Red Blood Cells, Plasmodium extracts
Machado, Patrícia Isabel Pires.
Pyruvate kinase and glucose-6-phosphate dehydrogenase deficiencies and their association with malaria–population genetics and proteomic studies . Diss. Universidade do Porto, 2013.

Walpurgis, Katja, et al. " Effects of gamma irradiation and 15 days of subsequent ex vivo storage on the cytosolic red blood cell proteome analyzed by 2D DIGE and Orbitrap MS ." PROTEOMICS-Clinical Applications (2013).

P. Falciparum Clone 3D7 Cultured In Human Erythrocytes
Lasonder E, Green JL, Camarda G, Talabani H, Holder AA, Langsley G, Alano P.
The Plasmodium falciparum schizont phospho-proteome reveals extensive phosphatidylinositol and cAMP-Protein Kinase A signalling . J Proteome Research. 2012;

Red Blood Cell Lysate
Barasa, Benjamin, and Monique Slijper. "
Challenges for red blood cell biomarker discovery through proteomics ." Biochimica et Biophysica Acta (BBA)-Proteins and Proteomics 1844.5 (2014): 1003-1010.

Lange, Philipp F., Pitter F. Huesgen, Karen Nguyen, and Christopher M. Overall. "
Annotating N termini for the Human Proteome Project: N termini and Nα-acetylation status differentiate stable cleaved protein species from degradation remnants in the human erythrocyte proteome ." Journal of proteome research (2014).

Katja Walpurgis, Maxie Kohler, Andreas Thomas et al.
Validated hemoglobin-depletion approach for red blood cell lysate proteome analysis by means of 2D-PAGE and Orbitrap MS .Electrophoresis.2012;

Mizukawa, B., George, A., Pushkaran, S. et al. Cooperating G6PD mutations associated with severe neonatal hyperbilirubinemia and cholestasis .Pediatric Blood Cancer.2011;56: 840-842.

Sudha Neelam, David G Kakhniashvili, Stephan Wilkens et al. Functional 20S proteasomes in mature human red blood cells Experimental Biology and Medicine.2011;236:580-591

Delipidation

Cleanascite™ References



Pancreatic Rough Endoplasmic Reticulum (RER) Fractions


Waldron, Richard T., Hsin-Yuan Su, Honit Piplani, Joseph Capri, Julian P. Whitelegge, Kym F. Faull, Sugunadevi Sakkiah et al. "Ethanol Induced Disordering of Pancreatic Acinar Cell Endoplasmic Reticulum: An ER Stress/Defective Unfolded Protein Response Model." Cellular and Molecular Gastroenterology and Hepatology 5, no. 4 (2018): 479-497



Human SZ95 sebocytes


Lovászi, M., et al. "Sebum lipids influence macrophage polarization and activation." British Journal of Dermatology (2017). doi: 10.1111/bjd.15754



Ascites Fluid

Chan, DW, Mak, SL, Ngan, HYS. The significance of lipid metabolism in peritoneal metastases of ovarian cancer. The 2016 Cold Spring Harbour Asia Conference on Cancer and Metabolism, Suzhou, China, 19-23 September 2016. http://hub.hku.hk/handle/10722/235385

Serum 


Taylor, Steven W., et al. "A high-throughput mass spectrometry assay to simultaneously measure intact insulin and C-peptide." Clinica Chimica Acta (2016).

Serum or Blood


Berggren, Per Olof, and Shao-Nian Yang. "
Methods for treating and/or limiting development of diabetes." U.S. Patent Application No. 14/995,341.


Bile

Laohaviroj, Marut, et al. "A comparative proteomic analysis of bile for biomarkers of cholangiocarcinoma." Tumor Biology 39.6 (2017): 1010428317705764.


Vesterhus, Mette, Anders Holm, Johannes Roksund Hov, Ståle Nygård, Erik Schrumpf, Espen Melum, Liv Wenche Thorbjørnsen et al. " "Novel serum and bile protein markers predict primary sclerosing cholangitis disease severity and prognosis." Journal of Hepatology (2017).."Journal of Hepatology (2017). 



Megger, Dominik A., et al. "One Sample, One Shot-Evaluation of sample preparation protocols for the mass spectrometric proteome analysis of human bile fluid without extensive fractionation." Journal of Proteomics (2016). 

Lukic, Natalija, et al. " 
An integrated approach for comparative proteomic analysis of human bile reveals overexpressed cancer-associated proteins in malignant biliary stenosis.Biochimica et Biophysica Acta (BBA)-Proteins and Proteomics1844.5 (2014): 1026-1033.


Danese, Elisa, et al. " 
Assessment of bile and serum mucin5AC in cholangiocarcinoma: Diagnostic performance and biologic significance."Surgery (2014).


Farina, Annarita, et al. " 
Bile carcinoembryonic cell adhesion molecule 6 (CEAM6) as a biomarker of malignant biliary stenoses.Biochimica et Biophysica Acta (BBA)-Proteins and Proteomics (2013).


Boschetti, Egisto, and Pier Giorgio Righetti. 
Low-Abundance Proteome Discovery: State of the Art and Protocols. Newnes, 2013.


Wang W, Ai KX, Yuan Z, Huang XY, Zhang HZ. 
Different Expression of S100A8 in Malignant and Benign Gallbladder Diseases.Digestive diseases and sciences. 2012.


Hauser-Davis RA, Lima AA, Ziolli RL, Campos RC. 
First-time report of metalloproteinases in fish bile and their potential as bioindicators regarding environmental contamination.Aquatic Toxicology.2012;110-111:99-106


Farina A, Dumonceau JM, Frossard JL. 
Proteomic Analysis of Human Bile from Malignant Biliary Stenosis Induced by Pancreatic Cancer Journal of Proteome Research.2009; 8(1):159-69


Guerrier L, Claverol S, Finzi L et al. 
Contribution of solid-phase hexapeptide ligand libraries to the repertoire of human bile proteins. Journal of Chromatography.2007;1176(1-2):192-205


Chen Bo, Zheng Jian-wei, Wang Jian-ming, et al. 
Establishment and preliminary analysis of a 2-D human biliary map. Chinese Journal of Hepatobiliary Surgery.2007


Chen B, Dong JQ, Chen YJ et al 
Two-dimensional electrophoresis for comparative proteomic analysis of human bile. Hepatobiliary & pancreatic diseases international.2007 Aug;6(4):402-6


Guerrier L, Claverol S, Finzi L et al 
Contribution of solid-phase hexapeptide ligand libraries to the repertoire of human bile proteins.Journal of Chromatography A.2007;1176(1-2):192-205


Kristiansen TZ, Bunkenborg J, Gronborg M et al 
A Proteomic Analysis of Human Bile Molecular and Cellular Proteomics.2004;3:715-728


Biological Matrices



Graeme T Clark, Paul J Russell, and Steven Westwood. Modification without impact: a case study in clinical assay failure due to lipemia. Bioanalysis; 2012: 4,(12):1421-1428


Egg Yolk

Ben Wade, Michelle Cummins, Anthony Keyburn and Tamsyn M. Crowley. Isolation and detection of microRNA from the egg of chickens. BMC Research Notes 2016 9:283.



Organ Homogenates

Myerson, J., He, L., Lanza, G., Tollefsen, D. and Wickline, S. Thrombin-inhibiting perfluorocarbon nanoparticles provide a novel strategy for the treatment and magnetic resonance imaging of acute thrombosis. Journal of Thrombosis and Haemostasis.2011;9:1292-1300



Thakuria D, Schmidt O, Liliensiek AK. Field preservation and DNA extraction methods for intestinal microbial diversity analysis in earthworms. Journal of Microbiological Methods.2009;76(3):226-33



Cheng AM, Moore EE, Masuno T et al Normal Mesenteric Lymph Blunts the Pulmonary Inflammatory Response to Endotoxin. Journal of Surgical Research.2006;136(S2):166-171



McNally T, Mackie IJ, Machin SJ et al. Increased levels of beta 2 glycoprotein I antigen and beta 2 glycoprotein I binding antibodies are associated with a history of thromboembolic complications in patients with SLE and primary antiphospholipid syndrome British journal of rheumatology.1995 Nov;34(11):1031-6


Red Blood Cells

Antunes RF; Brandao C; Maia M; Arosa FA. Red blood cells release factors with growth and survival bioactivities for normal and leukemic T cells. Immunology and Cell Biology.2011;89(1):111-21


Tracheal Swab Samples

Li D, Wang J, Wang R, Li Y. A nanobeads amplified QCM immunosensor for the detection of avian influenza virus H5N1, Biosensors and Bioelectronics.2011;26(S10):4146-4154



Fu LM, Shinnick TM. Genome-wide exploration of the drug action of capreomycin on Mycobacterium tuberculosis using Affymetrix oligonucleotide GeneChips Journal of Infection.2007;54(S3):277-284



Fu LM, Shinnick TM. Genome-wide analysis of intergenic regions of mycobacterium tuberculosis H37Rv using affymetrix genechips. EURASIP journal on bioinformatics & systems biology.2007:23054


Tissue and Cell Culture


United States Patent Application 20170348398 entitled: "COMPOSITIONS AND METHODS FOR DECREASING BLOOD GLUCAGON LEVELS”  

Ahmed, Syed Mukhtar, and Ian G. Macara The Par3 polarity protein is an exocyst receptor essential for mammary cell survival.. Nature Communications.8 (2017): 14867. doi:10.1038/ncomms14867



Alhamdani MS, Schroder C, Hoheisel JD. Analysis conditions for proteomic profiling of mammalian tissue and cell extracts with antibody microarrays. Proteomics.2010;10(17):3203-7



Czambel RK, Kharlamov A, Jones SC. Variations of brain endothelial nitric oxide synthase concentration in rat and mouse cortex.Nitric Oxide.2010;22(S1): 51-57




Plasma/Serum

McIntyre, John A., et al. " Antiphospholipid autoantibodies as blood biomarkers for detection of early stage Alzheimer's disease.Autoimmunity0 (2015): 1-8.



Knight, Jason S., Wei Luo, Alexander A. O'Dell, Srilakshmi Yalavarthi, Wenpu Zhao, Venkataraman Subramanian, Chiao Guo et al. " Peptidylarginine Deiminase Inhibition Reduces Vascular Damage and Modulates Innate Immune Responses in Murine Models of Atherosclerosis.Circulation research (2014): CIRCRESAHA-113.



Palekar, Rohun U., et al. " Thrombin-Targeted Liposomes Establish A Sustained Localized Anticlotting Barrier Against Acute Thrombosis." Molecular pharmaceutics (2013).



Lijowski M, Caruthers S, Hu G. High-Resolution SPECT-CT/MR Molecular Imaging of Angiogenesis in the Vx2 Model. Investigative Radiology.2009;44(1): 15–22



Turner JD, Langley RS, Johnston KL. Wolbachia Lipoprotein Stimulates Innate and Adaptive Immunity through Toll-like Receptors 2 and 6 to Induce Disease Manifestations of Filariasis The Journal of Biological Chemistry.2009;284:22364-22378



Torrelles JB, DesJardin LE, MacNeil J. et al Inactivation of Mycobacterium tuberculosis mannosyltransferase pimB reduces the cell wall lipoarabinomannan and lipomannan content and increases the rate of bacterial-induced human macrophage cell death Glycobiology.2009;19(7):743-755



Cho N, Chueh PJ, Kim C et al Monoclonal antibody to a cancer-specific and drug-responsive hydroquinone (NADH) oxidase from the sera of cancer patients. Cancer Immunology, Immunotherapy. 2002;51(3):121-9



Shapiro S, Beenhouwer DO, Feldmesser M et al. Immunoglobulin G Monoclonal Antibodies to Cryptococcus neoformans Protect Mice Deficient in Complement Component C3 Infect. Infection and immunity.2002;70(5):2598-604



Castro AR, Morrill We, Pope V. Lipid Removal from Human Serum Samples Clinical and diagnostic laboratory immunology.2000;7(2):197-199



Nussbaum G, Cleare W, Casadevall A et al Epitope Location in the Cryptococcus neoformans Capsule Is a Determinant of Antibody Efficacy The Journal of experimental medicine.1997;185:685-694


Nanoparticles

Human & Plasma Cell Lines 
Pan, Dipanjan, et al. "
A strategy for combating melanoma with oncogenic c-Myc inhibitors and targeted nanotherapy.Nanomedicine 10.2 (2015): 241-251.



Plasma-Enzyme-Nanoparticle Mixture 
Pan, Dipanjan, et al. "Anti-Angiogenesis Therapy in the Vx2 Rabbit Cancer Model with a Lipase-cleavable Sn 2 Taxane Phospholipid Prodrug using αvβ3-Targeted Theranostic Nanoparticles.Theranostics 2014; 4(6):565-578


Microarray

Srinivasan, Harish."Antibody microarray as a proteomic tool for effective diagnosis and prediction of prognosis in cancer."(2014).


Saliva

Lucy E. DesJardin Isolation of M. tuberculosis RNA from Sputum Methods in Molecular Medicine.2001;48:133-139



Beenhouwer DO, Shapiro S, Feldmesser M et al. Both Th1 and Th2 Cytokines Affect the Ability of Monoclonal Antibodies To Protect Mice against Cryptococcus neoformans Infection and immunity.2001;69: 6445-6455



Desjardin LE, Perkins MD, Wolski K et al. Measurement of Sputum Mycobacterium tuberculosis Messenger RNA as a Surrogate for Response to Chemotherapy American journal of respiratory and critical care medicine.1999;160(1):203-10



Template Preparation Production Sequencing Protocols. Stanford Genome Technology Center


Patents

Iwakura Yoichiro, Kakuta Shigeru, Suzuki, Shunsuke - United States Patent Application 20130011413. Method and Pharmaceutical Composition for Treatment of Intestinal Disease



Mcintyre, John A. United States Patent: 20120107841. Serum Diagnostic Method, Biomarker and Kit for Early Detection and Staging of Alzheimer's Disease

Morre, James D et al. United States Patent: 20030170757. Monoclonal antibodies specific for neoplasia-specific NADH: disulfide reductase



DJ Morre, NM McCarty, D Morre et al United States Patent: 7053188. Monoclonal antibodies specific for neoplasia-specific NADH: disulfide reductase



David C. Jones. United States Patent: 7999084. Devices and methods for reducing matrix effects



J Krupey - United States Patent: 5885921 Hydrophobic silica adsorbents for lipids


Vaccine Research (Cholesterol Removal From Human Serum)

Kamtchoua, Thierry, Monica Bologa, Robert Hopfer, David Neveu, Branda Hu, Xiaohua Sheng, Nicolas Corde, Catherine Pouzet, Gloria Zimmerman, and Sanjay Gurunathan. Safety and immunogenicity of the pneumococcal pneumolysin derivative PlyD1 in a single-antigen protein vaccine candidate in adults.Vaccine (2012).



Yeast assays

Lifang, et al. Balanced globin protein expression and heme biosynthesis improve production of human hemoglobin in Saccharomyces cerevisiae. Metabolic Engineering (2013).


Protein Removal

Protein Removal for Drug Screening & Metabolomics

NuGel™ BindPro™ - Protein Removal

Lipoproteins


Turner, Joseph D., R. Stuart Langley, Kelly L. Johnston, Katrin Gentil, Louise Ford, Bo Wu, Maia Graham et al. " Wolbachia lipoprotein stimulates innate and adaptive immunity through Toll-like receptors 2 and 6 to induce disease manifestations of filariasis. " Journal of Biological Chemistry 284, no. 33 (2009): 22364-22378.


Patent


Bhogal, John, Shridhara Alva Karinka, Timothy P. Henning, David Cunningham, Udo Hoss, Andrew H. Naegeli, and John Latour. " Methods of Collecting and Analyzing Samples ." U.S. Patent 20,120,296,189, issued November 22, 2012.

BindPro™ For Drug Binding/Screening

BindPro™ Trial Kit

Solid-phase Protein Extraction Reagent / DNA Isolation / Microbial & Proteoglycan Enrichment

ProCipitate™

Review

ProCipitate™, Superior Substitute to Phenol/Chloroform for DNA Isolation & Protein Extraction . by Matt Kuruc February 23, 2015


Patents

U.S. Patent Numbers 5,294,681, 5,453,493 and other patents pending.

U.S. Patent Number 5,538,870, Method for Preparing Nucleic Acids For Analysis And Kits Useful Therefore .


Enteric Viruses

Richards, Gary P., and Gail E. Greening. " Detection of enteric viruses in shellfish ." Molluscan Shellfish Safety . Springer Netherlands, 2014. 177-183.


Schwab KJ; De Leon R; Sobsey MD. Immunoaffinity concentration and purification of waterborne enteric viruses for detection by reverse transcriptase PCR . Applied and Environmental Microbiology.1996;62(6):2086-94


Schwab KJ, De Leon R, Sobsey MD Concentration and purification of beef extract mock eluates from water samples for the detection of enteroviruses, hepatitis A virus, and Norwalk virus by reverse transcription-PCR . Applied and Environmental Microbiology.1995; 61(2): 531-537


LA Jaykus, R De Leon and MD Sobsey. A virion concentration method for detection of human enteric viruses in oysters by PCR and oligoprobe hybridization Applied and Environmental Microbiology.1996; 62(6): 2074–2080


Mycobacterium Avium Paratuberculosis

Charles G. Thornton; Kerry M. MacLellan; Judith R. Stabel; Christine Carothers; Robert H. Whitlock; Selvin Passen Application of the C18-Carboxypropylbetaine Specimen Processing Method to Recovery of Mycobacterium avium subsp. paratuberculosis from Ruminant Tissue Specimens Journal of Clinical Microbiology.2002;40:5;1783-1790


Zoltan S.; Gyimesi; Ilse H.; Stalis; Janice M.; Miller; Charles O Detection of Mycobacterium avium Subspecies avium in Formalin-Fixed, Paraffin-Embedded Tissues of Captive Exotic Birds Using Polymerase Chain Reaction . Thoen Journal of Zoo and Wildlife Medicine.1999;30:3:348-353


Miller, J.M., et al, Polymerase chain reaction identification of Mycobacterium avium in formalin-fixed, paraffin-embedded animal tissues. Journal of veterinary diagnostic investigation.1999.11:436-440


Paraffin-embedded tissues


Ellingson, J. L. E., et al. " Detection of Mycobacterium avium subspecies paratuberculosis in free-ranging bison (Bison bison) by PCR ."Molecular and cellular probes 19.3 (2005): 219-225.


Recombinant Bacterial Colonies


O. Umesh K. Reddy; Alan E. Pepper; Ibrokhim Abdurakhmonov; Sukumar Saha; Johnie N. Jenkins; Thomas Brooks, Yuksel Bolek; Kamal M. El-Zik New Dinucleotide and Trinucleotide Microsatellite Marker Resources The Journal of Cotton Science.2001;5:103-113

Quiniou SM; Katagiri T; Miller NW; Wilson M; Wolters WR; Waldbieser GC Construction and characterization of a BAC library from a gynogenetic channel catfish Ictalurus punctatus . Genetics, selection, evolution : GSE. 2003;35(6):673-83


Bacterial artificial chromosome (BAC) vector


J M Kelley; C E Field; M B Craven; D Bocskai; U J Kim; S D Rounsley; M D Adams. High Throughput Direct End Sequencing of BAC Clones . Nucleic Acids Research.1999.15;27(6):1539-1546


Carcinoma Cell Lines & Bacterial Artificial Chromosomes (BACs)


Alsop, Amber E., Andrew E. Teschendorff, and Paul AW Edwards. " Distribution of breakpoints on chromosome 18 in breast, colorectal, and pancreatic carcinoma cell lines ." Cancer genetics and cytogenetics 164.2 (2006): 97-109.


Plasmid Deoxyribonucleic acid (DNA)


G.M. Huang; K. Wang; C.L. Kuo; B. Paeper; L. Hood
A High-Throughput Plasmid DNA Preparation Method Analytical Biochemtry.1994;15;223(1):35-8


Robert R. Klein, Daryl T. Morishige, Patricia E. Klein, Jianmin Dong; John E. Mullet. High Throughput BAC DNA Isolation for Physical Map Construction of Sorghum. Plant Molecular Biology Reporter.1988;16(4):651-364


Deoxyribonucleic acid (DNA)


Kozekov ID; Turesky RJ; Alas GR; Harris CM; Harris TM; Rizzo CJ. Formation of Deoxyguanosine Cross-Links from Calf Thymus DNA Treated with Acrolein and 4-Hydroxy-2-nonenal. Chemical Research in Toxicology.2010;23(11):1701-1713Applied Biosystems User Bulletin. Subject: Sequencing Large DNA Templates.

David C. Bruce; Mark O. Mundt; Kim K. McMurry; Linda J. Meincke; Donna L. Robinson; Norman A. Doggett; Larry L. Deaven. BAC Library End Sequencing in Support of Whole Genome Assemblies DOE Joint Genome Institute and Center for Human Genome Studies, Los Alamos National Laboratory


Dr. Domon, National Agricultural Research Center for Kyushu Okinawa Region, Japan, Extraction of Rush DNA, unpublished personal correspondence, 2004.

DNA Extraction Genetic Diversity as an Indicator of Ecosystem Condition and Sustainability for Regional Assessments of Stream Condition in the Eastern United States


Human Fibroblasts


Stephanie M Cohen, Terrence S Furey, Norman A Doggett, and David G Kaufman. Genome-wide sequence and functional analysis of early replicating DNA in normal human fibroblasts BMC Genomics.2006;7: 301


Norovirus, Adenovirus


Roberto A. Rodríguez, Lauren Thie, Christopher D. Gibbons, Mark D. Sobsey. Reducing the effects of environmental inhibition in quantitative real-time PCR detection of adenovirus and norovirus in recreational seawaters. Journal of Virological Methods;2012:181(S1):43-50


Concentration and purification of beef extract mock eluates from water samples for the detection of enteroviruses, hepatitis A virus, and Norwalk virus by reverse transcription-PCR . Applied and environmental microbiology.1995;61(2):531-7


Blood


Krupey, J., et al, 100,000+ PCRs Possible from 10 ml Blood , poster Biotechniques Symposium, 2003.


Human T-cell lymphotrophic virus I-induced adult T-cell leukemia (ATL)


J D Burton; R N Bamford; C Peters; A J Grant; G Kurys; C K Goldman; J Brennan; E Roessler; T A Waldmann. A lymphokine, provisionally designated interleukin T and produced by a human adult T-cell leukemia line, stimulates T-cell proliferation and the induction of lymphokine-activated killer cells Proceedings of the National Academy of Sciences of the United States of America.1994. 91(11): 4935-4939

Zebrafish genomic library of bacterial artificial chromosome (BAC) clones and BAC DNA

McDermott, Brian M., et al. Transgenic labeling of hair cells in the zebrafish acousticolateralis system . Gene Expression Patterns 10.2 (2010): 113-118.



Virus Enrichment

Virus and Viral Component Isolation

Viraffinity™

Patents

Parthasarathy, Ranjani V., Hsi-Chou Liu, Wensheng Xia, Assumpta Bennaars-Eiden, Paul N. Holt, William Bedingham, Christopher R. Kokaisel, and Jeffrey C. Pederson. " Methods for nucleic acid amplification ." U.S. Patent Application 12/596,346, filed April 25, 2008.

Parthasarathy, Ranjani, Katya Ericson, and William Bedingham. " Methods for nucleic acid isolation and kits using a microfluidic device and sedimenting reagent ." U.S. Patent Application 10/852,022, filed May 24, 2004.

Bedingham, William, et al. " Variable valve apparatus and methods ." U.S. Patent No. 8,057,758. 15 Nov. 2011.

Parthasarathy, Ranjani, Katya Ericson, and William Bedingham. " Methods for nucleic acid isolation and kits using solid phase material ." U.S. Patent Application No. 10/852,645.

Parthasarathy, Ranjani V., Katya Ericson, and William Bedingham. " Methods for nucleic acid isolation and kits using a microfluidic device and concentration step. " U.S. Patent 7,939,249, issued May 10, 2011.

Bedingham, William, Barry Robole, Ranjani Parthasarathy, and Katya Ericson. "Variable Valve Apparatus and Methods." U.S. Patent Application 11/684,656, filed March 12, 2007.

Bedingham, William, et al. " Variable Valve Apparatus and Methods. " U.S. Patent Application No. 11/684,656.

European Patent Application EP2305809 Methods for nucleic acid isolation and kits using a microfluidic device and concentration step

Journal Citations

Dengue Virus

Fragnoud, Romain, et al. " Differential proteomic analysis of virus-enriched fractions obtained from plasma pools of patients with dengue fever or severe dengue ." BMC infectious diseases 15.1 (2015): 518.

Food Samples

Maurer, John, ed. PCR methods in foods . Springer Science & Business Media, 2006.

D’Souza, D. H. " Update on foodborne viruses: types, concentration and sampling methods. " Advances in Microbial Food Safety 2 (2014): 102.

Jaykus, Lee-Ann. " Detection of human enteric viruses in foods. " Foodborne Diseases Handbook 2 (2000): 137-163.

Sair, A. I., D. H. D'souza, and L. A. Jaykus. " Human enteric viruses as causes of foodborne disease ." Comprehensive reviews in food science and food safety 1.2 (2002): 73-89.

Lee, A. " Rapid Detection of Foodborne Viruses from Minimally Processed Foods , poster Australian Society of Microbiology." (2000).

Grohmann, Gary, and Alvin Lee. " Viruses, food and environment." Foodborne microorganisms of public health significance . Sydney: AIFST (1997): 603-620.

Savers, Screen. " EXHIBIT DESCRIPTIONS. " Microbial& Comparative Genomics 2.3 (1997): 244-249.

Use of Viraffinity and Multiplex RT-PCR for the dectection of Food borne viruses

Food and Water Samples

Grohmann, Gary, and Alvin Lee. " Viruses, food and environment." Foodborne microorganisms of public health significance. Sydney: AIFST (1997): 603-620.

Isometric Virus

Zeddam, Jean-Louis, et al. " An isometric virus of the potato tuber moth Tecia solanivora (Povolny)(Lepidoptera: Gelechiidae) has a tri-segmented RNA genome ." Journal of invertebrate pathology 99.2 (2008): 204-211.

Hantaan Virus

Yu, Hai-Tao, Hong Jiang, Ye Zhang, Xue-Ping Nan, Yu Li, Wei Wang, Wei Jiang et al. " Hantaan virus triggers TLR4-dependent innate immune responses ."Viral immunology 25, no. 5 (2012): 387-393.

Wang, Ping-Zhong, Chang-Xing Huang, Ye Zhang, Zhi-Dong Li, Hai-Tao Yu, Ying Zhang, Zhan-Sheng Jia et al. " Analysis of the immune response to Hantaan virus nucleocapsid protein C-terminal-specific CD8+ T cells in patients with Hemorrhagic fever with renal syndrome ."Viral immunology 22, no. 4 (2009): 253-260.

Jiang, Hong, Ping-Zhong Wang, Ye Zhang, Zhe Xu, Li Sun, Li-Mei Wang, Chang-Xing Huang et al. " Hantaan virus induces toll-like receptor 4 expression, leading to enhanced production of beta interferon, interleukin-6 and tumor necrosis factor-alpha. " Virology 380, no. 1 (2008): 52-59.

Human Enteric Viruses

Leggitt, Paris R., and Lee-ann Jaykus. " Detection methods for human enteric viruses in representative foods ." Journal of Food Protection® 63.12 (2000): 1738-1744.

Jaykus, Lee-Ann. " Detection of human enteric viruses in foods. " Foodborne Diseases Handbook 2 (2000): 137-163.

Human Mouth Virus

Al-Jarbou, Ahmed. Metagenomic Analysis of the Human Mouth Virus Population and Characterisation of Two Lytic Viruses . Diss. University of Leicester, 2009.

Lepidoptera: Gelechiidae

Zeddam, Jean-Louis, et al. " An isometric virus of the potato tuber moth Tecia solanivora (Povolny)(Lepidoptera: Gelechiidae) has a tri-segmented RNA genome ." Journal of invertebrate pathology 99.2 (2008): 204-211.

Serum

Moreau, Isabelle, John Levis, Orla Crosbie, Elizabeth Kenny-Walsh, and Liam J. Fanning et al. " Correlation between pre-treatment quasispecies complexity and treatment outcome in chronic HCV genotype 3a ." Virology journal 5, no. 1 (2008): 1.

Abdurahman, Samir. Studies on HIV-1 core assembly . Institutionen för laboratoriemedicin/Department of Laboratory Medicine, 2007.

Human Immunodeficiency Virus Type 1

Abdurahman, Samir, Akos Végvári, Masoud Youssefi, Michael Levi, Stefan Höglund, Elin Andersson, Peter Horal, Bo Svennerholm, Jan Balzarini, Anders Vahlne, et al. " Activity of the small modified amino acid α-hydroxy glycineamide on in vitro and in vivo human immunodeficiency virus type 1 capsid assembly and infectivity ." Antimicrobial agents and chemotherapy 52, no. 10 (2008): 3737-3744.

Abdurahman, S., Höglund, S., Höglund, A., & Vahlne, A et al,. (2007). Mutation in the loop C-terminal to the cyclophilin A binding site of HIV-1 capsid protein disrupts proper virus assembly and infectivity . Retrovirology, 4(1), 1.

Abdurahman, Samir, Masoud Youssefi, Stefan Höglund, and Anders Vahlne, et al. " Characterization of the invariable residue 51 mutations of human immunodeficiency virus type 1 capsid protein on in vitro CA assembly and infectivity ." Retrovirology 4, no. 1 (2007): 1.

Abdurahman, Samir, Stefan Höglund, Laura Goobar-Larsson, and Anders Vahlne . " Selected amino acid substitutions in the C-terminal region of human immunodeficiency virus type 1 capsid protein affect virus assembly and release ." Journal of general virology 85, no. 10 (2004): 2903-2913.

Plasma

Fragnoud, Romain, Marie Flamand, Frederic Reynier, Philippe Buchy, Vasna Duong, Alexandre Pachot, Glaucia Paranhos-Baccala, and Frederic Bedin. " Differential proteomic analysis of virus-enriched fractions obtained from plasma pools of patients with dengue fever or severe dengue ." BMC infectious diseases 15, no. 1 (2015): 518.

Polio Virus

Ting, W.T. E ., E. M. Nielson, and C.C. Tseng. 1997. The use of Viraffinity matrix to concentrate waterborne polioviruses for RT-PCR detection . abstr. Q-169. p.484. In Abstracts of the 97th General Meeting of the American Society for Microbiology 1997, American Society for Microbiology, Washington D.C.


Bacteriophage Lambda DNA

Hitti, J. "Fast and Convenient Purification of Bacteriophage Lambda DNA with Viraffinity™ Matrix, poster Cold Spring Harbor Conference on Genome Mapping & Sequencing." (1997).

ViraPrep™ Mammal


Metagenomic Analysis of the Human Mouth Virus Population and Characterisation of Two Lytic Viruses Ahmed N. Al-Jarbou, MSc University of Leicester



Functional & Chemical Proteomics

Compound Centric Chemical Proteomics (CCDP) & Protein Compression

NuGel™ NRicher™ Mx


Suggested References

The Functional & Chemical Proteomics Handbook 03/2016

Oka, Amita R., Matthew P. Kuruc, Ketan M. Gujarathi, and Swapan Roy. " Functional Proteomic Profiling of Phosphodiesterases Using SeraFILE Separations Platform. " International Journal of Proteomics 2012 (2012).


New Chemical Proteomic Methods To Access Drug-Protein Interactions

US HUPO 2014. Frontiers in Proteomics: Advancing Biology through Technology and Computation.

NuGel™ PROfessorabstract entitled " Compound-Centric Displacement Proteomics - An Advantaged Method To Survey Small Molecule-Protein Interactions " poster board 096 presented at US HUPO 2014

Functional Proteomics Separations and Enrichment Kit

NuGel™ Nricher™ 6

Polymer Coated Silica Affinity Immobilization Matrices

NuGel™ Poly-Carboxy

NuGel™ Poly-Hydroxy

NuGel™ Poly-Aldehyde

NuGel™ Poly-Amine

NuGel™ Poly-Epoxy


NuGel™ Related References


Patents

Monoclonal antibodies directed to the cytotoxic lymphocyte maturation factor European Patent EP0790255

Purification of immunoglobulins using affinity chromatography and peptide US 2006/0153834 A1


Affinity

Chaumet, Alexandre, Sandrine Castella, Laïla Gasmi, Aurélie Fradin, Gilles Clodic, Gérard Bolbach, Robert Poulhe, Philippe Denoulet, and Jean-Christophe Larcher. " Proteomic analysis of Interleukin enhancer binding factor 3 (Ilf3) and Nuclear Factor 90 (NF90) interactome. "Biochimie (2013).


Dermot Walls, Robert McGrath and Sinéad T.Loughran A Digest of Protein Purification . Methods Molecular Biology. Volume 681: 3-23 (2011)


Ehrlich, G. K., Michel, H., Chokshi, H. P. and Malick, A. W. Affinity purification and characterization of an anti-PEG IgM . Journal of Molecular Recognition , 22: 99–103 (2009).


Development of hepatitis B virus capsids into a whole-chain protein antigen display platform: New particulate Lyme disease vaccines .International Journal of Medical Microbiology Volume 298, Issues 1-2, 3 January 2008, Pages 135-142


A sensitive and high-throughput assay to detect low-abundance proteins in serum Hongtao Zhang, Xin Cheng, Mark Richter & Mark I Greene. Nature Medicine 12, 473 - 477 (2006)


Transformation of a L-peptide epitope into a D-peptide analog. Peptides Frontiers of Peptide Science American Peptide Symposia , 2002, Volume 5, Session XI, 769-770


Expression and folding of an antibody fragment selected in vivo for high expression levels in Escherichia coli cytoplasm . Research in Microbiology Volume 153, Issue 7, September 2002, Pages 469-474


Identification of model peptides as affinity ligands for the purification of humanized monoclonal antibodies by means of phage display Journal of Biochemical and Biophysical Methods Volume 49, Issues 1-3.2001


George K. Ehrlich, Pascal Bailon, Wolfgang Berthold. Phage Display Technology - Identification of Peptides as Model Ligands for Affinity Chromatograph y Affinity Chromatography Methods in Molecular Biology , 2000, Volume 147, 209-220

A Digest of Protein Purification and partial amino acid sequence of a 28 kDa cyclophilin-like component of the rat liver sigma receptor . Life Sciences , Volume 55, Issue 8, 1994.


Nachman, M., Azad, A. R. M. and Bailon, P. (1992), Efficient recovery of recombinant proteins using membrane-based immunoaffinity chromatography (MIC) . Biotechnology and Bioengineering , 40: 564–571.


Kinetic aspects of membrane-based immunoaffinity chromatography. Journal of Chromatography A Volume 597, Issues 1-2, 24 April 1992, Pages 167-172


Identification of model peptides as affinity ligands for the purification of humanized monoclonal antibodies by means of phage display . Methods in Molecular Biology, 2000, Volume 147, 209-220


Membrane-based receptor affinity chromatography . Journal of Chromatography A Volume 597, Issues 1-2, 24 April 1992, Pages 155-166 9th International Symposium on Affinity Chromatography and Biological Recognition


Ion Exchange

Levin W Protein Purification of recombinant human secretory phospholipase A2 (group II) produced in long-term immobilized cell culture .Expr Purif 1992 Feb;3(1):27-35.

Genomics

ProCipitate™ - Solid-phase Protein Extraction Reagent DNA Isolation / Microbial & Proteoglycan Enrichment


Review
ProCipitate™, Superior Substitute to Phenol/Chloroform for DNA Isolation & Protein Extraction . by Matt Kuruc February 23, 2015


Patents

U.S. Patent Numbers 5,294,681, 5,453,493 and other patents pending.

U.S. Patent Number 5,538,870, Method for Preparing Nucleic Acids For Analysis And Kits Useful Therefore .

Enteric Viruses

Richards, Gary P., and Gail E. Greening. " Detection of enteric viruses in shellfish ." Molluscan Shellfish Safety . Springer Netherlands, 2014. 177-183.

Schwab KJ; De Leon R; Sobsey MD. Immunoaffinity concentration and purification of waterborne enteric viruses for detection by reverse transcriptase PCR . Applied and Environmental Microbiology.1996;62(6):2086-94

Schwab KJ, De Leon R, Sobsey MD Concentration and purification of beef extract mock eluates from water samples for the detection of enteroviruses, hepatitis A virus, and Norwalk virus by reverse transcription-PCR . Applied and Environmental Microbiology.1995; 61(2): 531-537

LA Jaykus, R De Leon and MD Sobsey. A virion concentration method for detection of human enteric viruses in oysters by PCR and oligoprobe hybridization Applied and Environmental Microbiology.1996; 62(6): 2074–2080

Mycobacterium Avium Paratuberculosis

Charles G. Thornton; Kerry M. MacLellan; Judith R. Stabel; Christine Carothers; Robert H. Whitlock; Selvin Passen Application of the C18-Carboxypropylbetaine Specimen Processing Method to Recovery of Mycobacterium avium subsp. paratuberculosis from Ruminant Tissue Specimens Journal of Clinical Microbiology.2002;40:5;1783-1790

Zoltan S.; Gyimesi; Ilse H.; Stalis; Janice M.; Miller; Charles O Detection of Mycobacterium avium Subspecies avium in Formalin-Fixed, Paraffin-Embedded Tissues of Captive Exotic Birds Using Polymerase Chain Reaction . Thoen Journal of Zoo and Wildlife Medicine.1999;30:3:348-353

Miller, J.M., et al, Polymerase chain reaction identification of Mycobacterium avium in formalin-fixed, paraffin-embedded animal tissues. Journal of veterinary diagnostic investigation.1999.11:436-440

Paraffin-embedded tissues

Ellingson, J. L. E., et al. "
Detection of Mycobacterium avium subspecies paratuberculosis in free-ranging bison (Bison bison) by PCR ." Molecular and cellular probes 19.3 (2005): 219-225.

Recombinant Bacterial Colonies

O. Umesh K. Reddy; Alan E. Pepper; Ibrokhim Abdurakhmonov; Sukumar Saha; Johnie N. Jenkins; Thomas Brooks, Yuksel Bolek; Kamal M. El-Zik New Dinucleotide and Trinucleotide Microsatellite Marker Resources The Journal of Cotton Science.2001;5:103-113

Quiniou SM; Katagiri T; Miller NW; Wilson M; Wolters WR; Waldbieser GC Construction and characterization of a BAC library from a gynogenetic channel catfish Ictalurus punctatus . Genetics, selection, evolution : GSE. 2003;35(6):673-83

Bacterial artificial chromosome (BAC) vector

J M Kelley; C E Field; M B Craven; D Bocskai; U J Kim; S D Rounsley; M D Adams. High Throughput Direct End Sequencing of BAC Clones . Nucleic Acids Research.1999.15;27(6):1539-1546

Carcinoma Cell Lines & Bacterial Artificial Chromosomes (BACs)

Alsop, Amber E., Andrew E. Teschendorff, and Paul AW Edwards. "
Distribution of breakpoints on chromosome 18 in breast, colorectal, and pancreatic carcinoma cell lines ." Cancer genetics and cytogenetics 164.2 (2006): 97-109.

Plasmid Deoxyribonucleic acid (DNA)

G.M. Huang; K. Wang; C.L. Kuo; B. Paeper; L. Hood A High-Throughput Plasmid DNA Preparation Method Analytical Biochemtry.1994;15;223(1):35-8

Robert R. Klein, Daryl T. Morishige, Patricia E. Klein, Jianmin Dong; John E. Mullet. High Throughput BAC DNA Isolation for Physical Map Construction of Sorghum. Plant Molecular Biology Reporter.1988;16(4):651-364

Deoxyribonucleic acid (DNA)

Kozekov ID; Turesky RJ; Alas GR; Harris CM; Harris TM; Rizzo CJ. Formation of Deoxyguanosine Cross-Links from Calf Thymus DNA Treated with Acrolein and 4-Hydroxy-2-nonenal. Chemical Research in Toxicology.2010;23(11):1701-1713

Applied Biosystems User Bulletin. Subject: Sequencing Large DNA Templates.

David C. Bruce; Mark O. Mundt; Kim K. McMurry; Linda J. Meincke; Donna L. Robinson; Norman A. Doggett; Larry L. Deaven. BAC Library End Sequencing in Support of Whole Genome Assemblies DOE Joint Genome Institute and Center for Human Genome Studies, Los Alamos National Laboratory

Dr. Domon, National Agricultural Research Center for Kyushu Okinawa Region, Japan, Extraction of Rush DNA, unpublished personal correspondence, 2004.

DNA Extraction Genetic Diversity as an Indicator of Ecosystem Condition and Sustainability for Regional Assessments of Stream Condition in the Eastern United States

Human Fibroblasts

Stephanie M Cohen, Terrence S Furey, Norman A Doggett, and David G Kaufman. Genome-wide sequence and functional analysis of early replicating DNA in normal human fibroblasts BMC Genomics.2006;7: 301

Norovirus, Adenovirus

Roberto A. Rodríguez, Lauren Thie, Christopher D. Gibbons, Mark D. Sobsey. Reducing the effects of environmental inhibition in quantitative real-time PCR detection of adenovirus and norovirus in recreational seawaters. Journal of Virological Methods;2012:181(S1):43-50

Concentration and purification of beef extract mock eluates from water samples for the detection of enteroviruses, hepatitis A virus, and Norwalk virus by reverse transcription-PCR . Applied and environmental microbiology.1995;61(2):531-7

Blood

Krupey, J., et al, 100,000+ PCRs Possible from 10 ml Blood , poster Biotechniques Symposium, 2003.

Human T-cell lymphotrophic virus I-induced adult T-cell leukemia (ATL)

J D Burton; R N Bamford; C Peters; A J Grant; G Kurys; C K Goldman; J Brennan; E Roessler; T A Waldmann. A lymphokine, provisionally designated interleukin T and produced by a human adult T-cell leukemia line, stimulates T-cell proliferation and the induction of lymphokine-activated killer cells Proceedings of the National Academy of Sciences of the United States of America.1994. 91(11): 4935-4939

Zebrafish genomic library of bacterial artificial chromosome (BAC) clones and BAC DNA

McDermott, Brian M., et al. Transgenic labeling of hair cells in the zebrafish acousticolateralis system . Gene Expression Patterns 10.2 (2010): 113-118.

Featured Applications

Featured Application - Bilirubin Analysis



Haree K. Pallera, Pamela S. Hair, Magdielis Gregory Rivera, Tushar A. Shah, Alice L. Werner,Frank A. Lattanzio, Kenji M. Cunnion, and Neel K. Krishna. Peptide inhibitor of complement C1 modulates acute intravascular hemolysis of mismatched red blood cells in rats.TRANSFUSION Volume 00, May 2016. doi:10.1111/trf.13674.

In brief, the study evaluated the role of the a peptide inhibitor of complement C1 (PIC1) in an animal model of acute intravascular hemolysis in both prevention and rescue scenarios. The authors state "To remove free Hb that may cause optical interference in bilirubin analysis, we treated all the samples with Hb depletion from hemolyzed serum/plasma (HemogloBind, Biotech Support Group). Bilirubin concentration was then measured with a Bilirubin Assay Kit (Sigma-Aldrich, St. Louis, MO)."



Featured Application - Whole Blood



Thomas H. Snider, Kevin G. McGarry, Michael C. Babin, David A. Jett, Gennady E. Platoff Jr., and David T. Yeung. Acute Toxicity of Phorate Oxon by oral gavage in the Sprague-Dawley rat. Fundam. Toxicol. Sci. Vol. 3, No.5, 195-204, 2016.

The article describes studies of the oral toxicity of Phorate Oxon with emphasis on gender and age-related effects in rats. The authors used HemogloBind™ as part of the protocol to measure cholinesterase activity. The article states “…80 µl aliquots of whole blood were removed, diluted and treated with HemogloBind™ to remove hemoglobin for later assay to detect cholinesterase activity…



Featured Application - Macromolecular Complexes

C Wan, B Borgeson, S Phanse, F Tu, K Drew, G Clark, et al. Panorama of ancient metazoan macromolecular complexes. Nature Volume:525, Pages:339–344 Date published:(17 September 2015). doi:10.1038/nature14877

Two of BSG products, NRicher™ 6 and HemogloBind™, were able to contribute to this rigorous examination of protein complexes. When our products were used as a pretreatment step in the overall workflow, about twice the number of observations and annotations became possible. This further validates that the sub-proteome bias characteristics of NRicher™ 6 can simplify complex proteomes into less complex sub-proteomes with efficiencies suitable for deep functional proteome characterization. Furthermore, this study demonstrated the importance of a key feature implicit to all of our products; that is the maintenance of functional and structural integrity after separations. Without that particular feature, these additional observations would not have been possible.



Human Peripheral Blood Mononuclear Cells (PBMCs)


Rubio-Navarro, Alfonso, et al."Hemoglobin induces monocyte recruitment and CD163-macrophage polarization in abdominal aortic aneurysm."International Journal of Cardiology (2015)."


A research article by authors Rubio-Navarro, Alfonso, et al in the journal International Journal of Cardiology (http://www.internationaljournalofcardiology.com/article/S0167-5273(15)30284-9/fulltext) cites Biotech Support Group’s HemogloBind™ sample preparation reagent to deplete hemoglobin (Hb) from conditioned medium from healthy aortas or abdominal aortic aneurysm. The article states:"Conditioned mediums from AAA were incubated with HemogloBind™ reagent for hemoglobin depletion".


In brief, authors describe high infiltration of CD163 monocytes surrounding micro-vesicles, low expression of CD14+ & CD16- monocytes and high CD163 mRNA/protein expression is a feature of abdominal aortic aneurysm (AAA) molecular pathology. Healthy aorta conditioned medium or complete or hemoglobin-depleted conditioned medium from abdominal aortic aneurysm were mixed with M-CSF macrophages to track CD163 and HLA-DR expression or hemoglobin uptake.


We are pleased with this data on abdominal aortic aneuryism & hemoglobin proteomics. HemogloBind™ minimizes hemoglobin interference from blood samples and allows research on cardiovascular pathology” states Swapan Roy, Ph.D., President and Founder of Biotech Support Group.


Dried Blood Spot(DBS)/Whole Blood


Hakuna, Lovemore, et al. "A simple assay for glutathione in whole blood."Analyst (2015).

A research article in the journal Analyst (http://pubs.rsc.org/en/content/articlelanding/2015/an/c5an00345h) cites Biotech Support Group’s HemogloBind™ sample preparation reagent to deplete hemoglobin (Hb) from whole blood samples containing glutathione to minimize interference from Hb in GSH fractions. Using a resorufin-acrylate fluorescent probe, GSH is quantitated in deproteinzed blood plasma and whole blood samples. The article states:

"Apart from dilution, Hb can be removed using a commercial product, HemogloBind™, which can isolate and remove up to 90% of blood Hb." Glutathione (GSH) is an antioxidant involved on nitric oxide regulation, covalent hemoglobin binding, DNA binding, leukotriene synthesis, protein synthesis and sepsis pathways. GSH is elevated in cancer tissues and proper minimally invasive GSH sample preparation from dried blood samples (DBS) allows research on neurodegenerative diseases, chronic respiratory diseases and diabetes. Authors Lovemore, et al cite a DBS method to fractionate GSH from blood components, deproteinization by filtration and depletion of hemoglobin enhances quantification & identification of GSH in whole blood samples. Then size exclusion chromatography or fluorescence imaging detection is performed for quantitative analysis and glutathione assay research.



Featured Application - Virus Proteomics

Romain Fragnoud, Marie Flamand, Frederic Reynier, Philippe Buchy, Vasna Duong, Alexandre Pachot, Glaucia Paranhos-Baccala and Frederic Bedin. Differential proteomic analysis of virus enriched fractions obtained from plasma pools of patients with dengue fever or severe dengue. BMC Infectious Diseases (2015) 15:518.http://www.ncbi.nlm.nih.gov/pubmed/26572220



In brief, the article’s authors report a method to compare the proteomes of virion-enriched fractions purified from plasma pools of patients with dengue fever or severe dengue. Virions were purified by ultracentrifugation combined with Viraffinity™. Following in-gel hydrolysis, peptides were analyzed by LC-MS. The article states “A technique based on ultracentifugation (Step 1) followed by concentration using…Viraffinity™, was created to obtain a fraction of plasma enriched with DV particles. A positive signal corresponding to monomeric (60 kDa) and dimeric E protein (120 KDa) was observed in the DV-infected samples, and became more intense after the Viraffinity™ step…Densitometry indicated that the protein complexity of the purified samples was reduced by roughly 350-fold compared to the unpurified samples.”. The authors conclude that the Viraffinity™ based technique of virion-enrichment allowed them to identify two host proteins that have prognostic value for classifying patients with acute dengue who are more likely to develop a severe dengue.



Featured Application - microRNA from Egg Yolk


Ben Wade, Michelle Cummins, Anthony Keyburn and Tamsyn M. Crowley. Isolation and detection of microRNA from the egg of chickens. BMC Research Notes 2016 9:283. DOI: 10.1186/s13104-016-2084-5


In brief, the article’s authors report a method for the reproducible and reliable isolation of miRNA from the albumen and yolk of chicken eggs. These methods will allow the investigation of epigenetic programming in chick development previously unknown, and how this impacts the nutritional value of eggs for human consumption. The article states “…400 µl aliquots of the yolk/lysis solution was dispensed into five 1.5 ml microcentrifuge tubes. To each of these aliquots 600 µl of Cleanascite™ was added followed by rigorous vortexing until the sample became homogenous. The Cleanascite™ removes the lipid from this high fat tissue that would otherwise interfere with the extraction process…"


Application Reports

Haiyan Zheng (1), Swapan Roy (2), Amenah Soherwardy (1), Seema Rahman (2), Matthew Kuruc (2); (1)Rutgers Center for Integrative Proteomics, Piscataway, NJ; (2)Biotech Support Group LLC, Monmouth Junction, NJ; entitled "Stroma Liquid Biopsy - Proteomic Profiles for Cancer Biomarkers "

Poster Reprint First Presented At AACR Annual Meeting 2016 Conference, Held April 17-20, 2016 New Orleans, LA USA. The Commonality of the Cancer Serum Proteome Phenotype as analyzed by LC-MS/MS, and Its Application to Monitor Dysregulated Wellness Haiyan Zheng 1; Caifeng Zhao 1; Swapan Roy 2; Devjit Roy MD; Amenah Soherwardy 2; Ravish Amin 2;Matthew Kuruc 21Rutgers Center for Integrative Proteomics, Piscataway, NJ; 2Biotech Support Group LLC, Monmouth Junction, NJ


The Functional Subproteomes of SERPIN Protease Inhibitors Are Now Open For LC-MS Biomarker Discovery


Haiyan Zheng; Caifeng Zhao; Swapan Roy; Devjit Roy MD; Amenah Soherwardy; Ravish Amin; Matthew Kuruc. Application Report - The Comparison of the Serum Proteome in Individuals with Cancers versus those without Cancer, and its application to Wellness


Kuruc Matt, Dr.Roy. Application Report - AlbuVoid™ LC-MS On-Bead – Differential Expression of Cancer Sera Proteins Using Quantitative (iTRAQ) Proteomics


Dr.Swapan Roy; Amenah Soherwardy; Ravish Amin; Matthew Kuruc.Monmouth Junction, NJ - Application Report. AlbuSorb™ Product Extension Combines Albumin and Immunoglobulin Depletion in a Consumable Format


ProCipitate™, Superior Substitute to Phenol/Chloroform for DNA Isolation & Protein Extraction . by Matt Kuruc February 23, 2015


The Functional & Chemical Proteomics Handbook


Hemoglobin Removal Reference Applications


Cleanascite™ Lipid Removal & Clarification Reference Applications


Albumin Removal Reference Applications

Liquid Biopsy


1.Haiyan Zheng 1; Swapan Roy 2; Devjit Roy MD 2; Amenah Soherwardy 1; Seema Rathman 2; Matthew Kuruc 21Rutgers Center for Integrative Proteomics, Piscataway, NJ; 2Biotech Support Group LLC, Monmouth Junction, NJ. Application Report: Stroma Liquid Biopsy – Pan-Cancer Dysregulation of the Serum Proteome.

2.Biotech Support Group described a panel of protein biomarkers dysregulated in cancer at the recent HUPO World Congress, that took place September 17-21 in Dublin, Ireland. This research shows that there are three common pathways which change in the bloodstream regardless of the primary tumor, stage, metastatic disease, or tumor burden. Five different cancers – lung, breast, pancreatic, lymph and ovary, were profiled along with normal/healthy individuals of approximate age and matched sex for comparison. The presentation is entitled “Stroma Liquid Biopsy – Biomarkers of the Dysregulation of the Serum Proteome in Cancer. It can be downloaded at: Biotech Support Group Presents Stroma Liquid Biopsy Cancer Data at HUPO World Congress 2017 .

3.Poster Reprint First Presented at New Jersey Cancer Retreat, May 25, 2017. Haiyan Zheng(1), Swapan Roy(2), Amenah Soherwardy(1), Seema Rahman(2), Matthew Kuruc(2); (1)Rutgers Center for Integrative Proteomics, Piscataway, NJ; (2)Biotech Support Group LLC, Monmouth Junction, NJ; entitled "Stroma Liquid Biopsy - Proteomic Profiles for Cancer Biomarkers" . Cell. 2011;144:646-674.

4.Zheng H, Zhao C, Roy S, et al. The Comparison of the Serum Proteome in Individuals with Cancers versus those without Cancer, and its application to Wellness . Poster reprint from 12th Annual US HUPO 2016 Conference, held March 13 – 16, 2016 Boston, MA, USA.

5.Zheng H, Zhao C, Roy S, et al. The Commonality of the Cancer Serum Proteome Phenotype as analyzed by LC-MS/MS, and Its Application to Monitor Dysregulated Wellness. Poster reprint from AACR Annual Meeting 2016 Conference, held April 17-20, 2016 New Orleans, LA USA

Patents (Third Party)
AlbuSorb™

Patent Number Title Date of Publication
US8557513B2 Methods for treating and/or limiting development of diabetes 10/15/13
WO2014095875A1 Methods for treating and/or limiting development of diabetes 06/26/14
US20170037118A1 Methods for treating and/or limiting development of diabetes 02/09/17
WO2014140113A1 Methods for treating and/or limiting development of diabetes 09/18/14



AlbuVoid™

US20150023949A1 Compositions and methods for diagnosis and treatment of pervasive developmental disorder 01/22/15
US20120258874 A1 Interrogatory cell-based assays and uses thereof 10/11/12
WO/2014/043421 Use of markers in the identification of cardiotoxic agents 03/20/14



BindPro™

EP2700934B1 Method for detecting a nucleic acid molecule in a biosample 12/14/16
US20120296189A1 Methods of Collecting and Analyzing Samples 11/22/12
EP2700934B1 Method for detecting a nucleic acid molecule in a biosample 12/14/16



Cleanascite™

US20120107841A1 Serum Diagnostic Method, Biomarker and Kit for Early Detection and Staging of Alzheimer's Disease 05/03/12
US20130011413A1 Method and Pharmaceutical Composition for Treatment of Intestinal Disease 01/10/13
US20170348398A1 Compositions and methods for decreasing blood glucagon levels 12/07/17
20020090663A1 Immunoassay for fish identification 07/11/02
20030170757A1 Monoclonal antibodies specific for neoplasia-specific NADH:disulfide reductase 09/11/03
9468607 Ligand directed toroidal nanoparticles for therapy and diagnostic imaging 10/18/16
9764043 Antithrombotic nanoparticle 09/19/17
WO/2007/084002A2 Antibiotics and methods for producing them 07/26/07
7999084 Devices and methods for reducing matrix effects 08/16/11
8257714 Compositions and methods for immunotherapy of cancer and infectious diseases



Hemoglobind™

US20090304778A1 Diagnostics and Methods for Removal and Detection of Interferents 12/10/09
20130224268A1 Topical delivery of hormonal and non hormonal nano formulations, methods of making and using the same 08/29/13
20040002594A1 Removal of extraneous substances from biological fluids containing nucleic acids and the recovery of nucleic acids 01/01/04



HemoVoid™

WO2017013664A1 Article of manufacture and methods for increasing survival of red blood cells 01/26/17



Nugel™ Poly-Hydroxy

US20120156757A1 Purification of immunogens using a non-polysaccharide matrix 06/21/12



ProCipitate™

US5538870A Method for preparing nucleic acids for analysis and kits useful therefor 07/23/96



Viraffinity™

US20100129878A1 Methods for nucleic acid amplification 05/27/10
US20050142571A1 Methods for nucleic acid isolation and kits using solid phase material 06/30/05
US20050130177A1 Variable valve apparatus and methods 06/16/05
US20050142570A1 Methods for nucleic acid isolation and kits using a microfluidic device and sedimenting reagent 06/30/05
US20050142663A1 Methods for nucleic acid isolation and kits using a microfluidic device and concentration step 06/30/05
CN1922318A Methods for nucleic acid isolation and kits using solid phase material 02/28/07
20160363589A1 Method and kit for determining the probability that a patient will develop a severe case of dengue 12/15/16
20040002594A1 Removal of extraneous substances from biological fluids containing nucleic acids and the recovery of nucleic acids 01/01/04
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