Serum/Plasma
Ching, J.J., Shuib, A.S., Majid, N.A., Taufek, N.M., Ismail, M.N. & Abdullah, N. 2025.Pleurotus pulmonarius stalk waste extract supplementation modulates serum proteins level in red hybrid tilapia Oreochromis sp. upon pamps challenge. ."Malaysian Applied Biology, 54(3): 76-88.
Intestinal Organoid Supernatants
Jordan, Christine KI, et al. "Symbiotic Firmicutes establish mutualism with the host via innate tolerance and resistance to control systemic immunity.."Cell Host & Microbe(2023).
Intestinal Organoid Supernatants
Matsuzawa-Ishimoto, Yu, et al. "The γδ IEL effector API5 masks genetic susceptibility to Paneth cell death.."Nature(2022): 1-8.
SerumExosomes
Valladolid-Acebes, Ismael, et al."Lowering apolipoprotein CIII protects against high-fat diet–induced metabolic derangements.." Science Advances7.11 (2021): eabc2931.
SerumExosomes
Chettimada,Sukrutha, et al. "Exosomemarkers associated with immune activation and oxidative stress in HIVpatients on antiretroviral therapy."ScientificReports8.1 (2018): 7227.
CerebrospinalFluid
GwenaelPottiez, Pawel Ciborowski. ProteomicProfiling of Cerebrospinal Fluid Expression Profiling InNeuroscienceNeuromethods.2012;64:245-270
Synovialfluid
HapponenKE, Fürst CM, Saxne T et al. PRELPprotein inhibits the formation of the complement membrane attackcomplex.Journal of Biological Chemistry.2012;287(11):8092-100
Serum
Vialaret, Jerome & Kadi, Sarah & Tiers, Laurent & O Flynn, Robin & Lehmann, Sylvain & Hirtz, Christophe. (2018). "Albumin depletion of human serum to improve quantitative clinical proteomics."Current Topics in Peptide & Protein Research 19. 53-62.
Roy, Swapan, and Matthew Kuruc. "Methods to Monitor the Functional Subproteomes of SERPIN Protease Inhibitors.Functional Proteomics. Humana Press, New York, NY, 2019. 41-54
Berggren,Per-olof, and Lisa Juntti-berggren. "Methodsfor treating and/or limiting development of diabetes." U.S.Patent No. 20,170,037,118. 9 Feb. 2017.MOJProteomics Bioinform 2016, 3(6): 00106
SwapanRoy, Matthew Kuruc.TheFunctional Subproteomes of Serpin Protease Inhibitors are Now Openfor LC-MS Biomarker Discovery.MOJProteomics Bioinform 2016, 3(6): 00106
HolmbergR, Refai E, Höög A. Loweringapolipoprotein CIII delays onset of type 1 diabetes.Proceedings of the National Academy of Sciences.2011;108(26):10685-9.
TangMX, Ogawa K, Asamoto M. Effectsof Nobiletin on PhIP-Induced Prostate and Colon Carcinogenesis inF344 RatsNutritionand Cancer.2011;63(2):227-33
Holmberg,Rebecka ApolipoproteinCIII and Ljungan virus in diabetes2010.Doctoral Thesis
LuQ, Zheng X, McIntosh T Developmentof different analysis platforms with LC-MS for pharmacokineticstudies of protein drugs.Analytical Chemistry.2009;81(21):8715-23
Urine
Zubiri,Irene, et al. "Diabeticnephropathy induces changes in the proteome of human urinary exosomesas revealed by label-free comparative analysis."Journal of proteomics 96 (2014): 92-102.
Patent
Berggren,Per Olaf, Yang, Shao-Nian. 2012. MethodsFor Treating And/Or Limiting Development Of Diabetes.U.S. Patent 20120328630 Kind Code: A1, filed June 25, 2012, andissued December 27, 2012.
CerebrospinalFluid
GwenaelPottiez, Pawel Ciborowski. ProteomicProfiling of Cerebrospinal Fluid Expression Profiling InNeuroscienceNeuromethods.2012;64:245-270
Synovialfluid
HapponenKE, Fürst CM, Saxne T et al. PRELPprotein inhibits the formation of the complement membrane attackcomplex.Journal of Biological Chemistry.2012;287(11):8092-100
Serum
HolmbergR, Refai E, Höög A. Loweringapolipoprotein CIII delays onset of type 1 diabetes.Proceedingsof the National Academy of Sciences.2011;108(26):10685-9.
TangMX, Ogawa K, Asamoto M. Effectsof Nobiletin on PhIP-Induced Prostate and Colon Carcinogenesis inF344 RatsNutritionand Cancer.2011;63(2):227-33
Holmberg,Rebecka ApolipoproteinCIII and Ljungan virus in diabetes2010.Doctoral Thesis
LuQ, Zheng X, McIntosh T Developmentof different analysis platforms with LC-MS for pharmacokineticstudies of protein drugs.Analytical Chemistry.2009;81(21):8715-23
Paulsen et al., ""Development of human induced pluripotent stem cell-derived ovarian support cells as a clinical-grade product for in vitro fertilization,"Cell Stem Cell (2025), https://doi.org/10.1016/j.stem.2025.12.020.
Golden, Lesley R., et al. ""APOE4 to APOE2 allelic switching in mice improves Alzheimer’s disease-related metabolic signatures, neuropathology and cognition."Nature Neuroscience (2025): 1-15.
Paulsen, Bruna, et al."Reproducible differentiation of pure ovarian support cells from clinical-grade hiPSCs as a novel infertility treatment."bioRxiv (2024): 2024-04.
Polack, M., et al. "Results from the UNITED study: a multicenter study validating the prognostic effect of the tumor–stroma ratio in colon cancer."ESMO open 9.4 (2024): 102988.
Sae-Lee, Wisath, et al. " The protein organization of a red blood cell ."Cell Reports 40.3 (2022): 111103.
Jenull,Sabrina, et al. "Thehistone chaperone HIR maintains chromatin states to control nitrogenassimilation and fungal virulence." Cell Reports 36.3(2021): 109406.
Osteogenic and Fibrogenic Blood Composites
Jing, Lun, et al. "PROTEOMIC ANALYSIS IDENTIFIED LBP AND CD14 AS KEY PROTEINS IN BLOOD/BIPHASIC CALCIUM PHOSPHATE MICROPARTICLE INTERACTIONS." Acta Biomaterialia, (2021).
Blood
TargetedLC-MS Proteomic Methods to Monitor and Quantify Stromal Conditioningin Cancer from Blood
MattKuruc1; Swapan Roy1; Wilma Mesker2; Rob Tollenaar2; 1BiotechSupport Group LLC, Monmouth Junction, NJ; 2Leiden UniversityMedical Center, Leiden, Netherlands
Kuruc M, Zheng H, Sowerhardy A, Avadhani S, Roy D, et al.(2020) "New Strategies to Categorize Blood for Proteomic Biomarker Discovery." Proteomics Bioinformatics, 2(2): 90-107.
Biotech Support Group to exhibit at the 67th ASMS Conference on Mass Spectrometry & Allied Topics.
HaiyanZheng1;Swapan Roy2;Devjit Roy MD2;Amenah Soherwardy1;Seema Rathman2;Matthew Kuruc21RutgersCenter for Integrative Proteomics, Piscataway, NJ; 2Biotech SupportGroup LLC, Monmouth Junction, NJStromaLiquid Biopsy – Pan-Cancer Dysregulation of the Serum Proteome
Serum
Vialaret, Jerome & Kadi, Sarah & Tiers, Laurent & O Flynn, Robin & Lehmann, Sylvain & Hirtz, Christophe."Albumin depletion of human serum to improve quantitative clinical proteomics.."Current Topics in Peptide & Protein Research 19. 53-62.
Roy, Swapan, and Matthew Kuruc."Methods to Monitor the Functional Subproteomes of SERPIN Protease Inhibitors.."Functional Proteomics. Humana Press, New York, NY, 2019. 41-54
Vialaret, Jerome, et al."Albumin depletion of human serum to improve quantitative clinical proteomics.."
Poillet-Perez, Laura, et al."Autophagy maintains tumour growth through circulating arginine.."Nature (2018): 1.
DavidL. Wang, Chuanguang Xiao, Guofeng Fu, Xing Wang and Liang Li."Identificationof potential serum biomarkers for breast cancer using a functionalproteomics technology.."Biomarker Research (2017) 5:11.. DOI 10.1186/s40364-017-0092-9
Sun,Zhenyu, Xiaofeng Chen, Gan Wang, Liang Li, Guofeng Fu, Matthew Kuruc,and Xing Wang. "Identificationof functional metabolic biomarkers from lung cancer patient serumusing PEP technology."Biomarker Research 4, no. 1 (2016): 1.
HaiyanZheng; Caifeng Zhao; Swapan Roy; Devjit Roy MD; Amenah Soherwardy;Ravish Amin; Matthew Kuruc. Application Report - TheComparison of the Serum Proteome in Individuals with Cancers versusthose without Cancer, and its application to Wellness
Dr.SwapanRoy; Amenah Soherwardy; Ravish Amin; Matthew Kuruc.Monmouth Junction,NJ - Application Report. AlbuSorb™Product Extension combines Albumin and Immunoglobulin Depletion in aConsumable Format
XingWang, Michael Davies, Swapan Roy and Matthew Kuruc. BeadBased Proteome Enrichment Enhances Features of the Protein ElutionPlate (PEP) for Functional Proteomic Profiling.Proteomes 2015, 3(4), 454-466. doi: 10.3390/proteomes3040454
Grubbs,J. K., et al. "Investigationof the efficacy of albumin removal procedures on porcine serumproteome profile1."(2015).
Discoveryof Functional Serum Biomarkers Using AlbuVoid™ Enrichment andthe ArrayBridge PEP Profiling Platform.Personal communication, Xing Wang, Ph.D., ArrayBridge (St. Louis,MO), manuscripts in process.
SerumProfiling Making Mark on Predictive Medicine
VickiGlaser.Genetic Engineering & Biotechnology News. 2011;31(7):1-55.
KurucMatt, Dr.Roy. ApplicationReport - AlbuVoid™ LC-MS On-Bead – DifferentialExpression of Cancer Sera Proteins Using Quantitative (iTRAQ)Proteomics
Patents
Narain, Niven Rajin, Rangaprasad Sarangarajan, and Vivek K. Vishnudas.""Interrogatory cell-based assays for identifying drug-induced toxicity markers.."U.S. Patent Application No. 16/180,446.
Narain,Niven Rajin, Rangaprasad Sarangarajan, and Vivek K. Vishnudas."INTERROGATORYCELL-BASED ASSAYS AND USES THEREOF."U.S. Patent No. 20,120,258,874. 11 Oct. 2012.
Plasma
Espes,Daniel, Joey Lau, and Per-Ola Carlsson. "Increasedcirculating levels of betatrophin in individuals with long-standingtype 1 diabetes."Diabetologia(2013): 1-4.
Patent- Secretome Sample Preparation
Narain,Niven Rajin, and Paula Patricia Narain. "COMPOSITIONSAND METHODS FOR DIAGNOSIS AND TREATMENT OF PERVASIVE DEVELOPMENTALDISORDER."U.S. Patent No. 20,150,023,949. 22 Jan. 2015.
Narain,Niven Rajin, Rangaprasad Sarangarajan, and Vivek K. Vishnudas."INTERROGATORYCELL-BASED ASSAYS AND USES THEREOF."U.S. Patent No. 20,120,258,874. 11 Oct. 2012.
CellCulture
Narain,Niven Rajin, Rangaprasad Sarangarajan, Vivek K. Vishnudas, andMichael Andrew Kiebish. "USEOF MARKERS IN THE IDENTIFICATION OF CARDIOTOXIC AGENTS AND IN THEDIAGNOSIS AND MONITORING OF CARDIOMYOPATHY AND CARDIOVASCULARDISEASE."U.S. Patent 20,140,100,128, issued April 10, 2014.
On-BeadDigestion Protocols For LC-MS Proteomic Workflows
Newon-bead digestion for LC-MSapplicationsfor proteomic studies
USHUPO 2014. Frontiers in Proteomics: Advancing Biology throughTechnology and Computation.
AlbuVoid™poster, entitled "Improvedproteomic enrichment and workflow strategies",poster board 089 presented at US HUPO 2014
Serum/Plasma
Sovakova, Magdalena, et al."Colchicine acutely reduces liver-secreted inflammatory and metabolic proteins in blood."European Journal of Pharmacology (2026): 178725.
Serum
Fraser DD, Roy S, Kuruc M, Quintero M, Van Nynatten LR, Cepinskas G, Zheng H, Soherwardy A and Roy D (2025)"Functional mass spectrometry indicates anti-protease and complement activity increase with COVID-19 severity."Exp. Biol. Med. 250:10308. doi: 10.3389/ebm.2025.10308
Plasma samples
Grantz, Jillian M., et al.""The platelet and plasma proteome and targeted lipidome in postpartum dairy cows with elevated systemic inflammation."" Scientific Reports 14.1 (2024): 31240.
Serum/PlasmaSamples
BiotechSupport Group & Lawson Health ResearchInstitute Enter Collaborative Research Agreement to Monitor ProteaseInhibitor Function During Covid-19 Infections
Serum
Thangavelu,Bharani, et al. "Datasetof Rat and Human Serum Proteomes Derived from Differential DepletionStrategies prior to Mass Spectrometry." Data in Brief(2020): 105657.
ApplicationReport
AlbuSorb™Product Extension combines Albumin and Immunoglobulin Depletion in aConsumable Format
References
ZhengH, Soherwardy A, Roy S, Kuruc M, Avadhani S. AlbuVoid™Enrichment & Antibody Depletion – Tacklingthe Challenges of Serum Proteomics Part II. Poster reprint firstpresented at 26th International Molecular Med TRI-CON,March10-15, 2019, San Francisco, CA USA
Vialaret,Jerome & Kadi, Sarah & Tiers, Laurent & O Flynn, Robin &Lehmann, Sylvain & Hirtz, Christophe. (2018). Albumindepletion of human serum to improve quantitative clinical proteomics.CurrentTopics in Peptide & Protein Research 19. 53-62.
Poillet-Perez,Laura, et al. "Autophagymaintains tumour growth through circulating arginine."Nature(2018): 1.
Roy,Swapan, and Matthew Kuruc. "Methodsto Monitor the Functional Subproteomes of SERPIN ProteaseInhibitors."Functional Proteomics. Humana Press, New York, NY, 2019. 41-54.
Mihara,Keisuke, et al. "Identificationof Specific Protein Markers of Rheumatoid Arthritis in Synovial Fluidand Serum."Journal of Hard Tissue Biology 27.1 (2018): 55-58.
DavidL. Wang, Chuanguang Xiao, Guofeng Fu, Xing Wang and LiangLi. Identificationof potential serum biomarkers for breast cancer using a functionalproteomics technology.BiomarkerResearch (2017) 5:11. DOI 10.1186/s40364-017-0092-9
Zheng,H., et al. "AlbuVoid™coupled to on-bead digestion-tackling the challenges of serumproteomics."JProteom Bioinformatics 8.9 (2015): 225.
Zheng,H., et al. Poster reprint first presented at 14th Human ProteomeOrganization World Congress (HUPO 2015), held on September 27 –30, 2015 entitled “NewProteomic Workflows Combine Albumin Depletion and On Bead Digestion,for Quantitative Cancer Serum”.
AlbuVoid™LC-MS On-Bead For Serum Proteomics
Serum
HaiyanZheng, Caifeng Zhao, Meiqian Qian, Swapan Roy, Absari Arpa, AmenahSoherwardy and Matthew Kuruc. AlbuVoid™Coupled to On-Bead Digestion - Tackling the Challenges of SerumProteomics. JProteomics Bioinform 2015, 8:9.
Grubbs,J. K., et al. " Investigationof the efficacy of albumin removal procedures on porcine serumproteome profile1."(2015).
Discoveryof Functional Serum Biomarkers Using AlbuVoid™ Enrichment andthe ArrayBridge PEP Profiling Platform.Personalcommunication, Xing Wang, Ph.D., ArrayBridge (St. Louis, MO),manuscripts in process.
SerumProfiling Making Mark on Predictive Medicine
VickiGlaser.Genetic Engineering & Biotechnology News. 2011;31(7):1-55.
KurucMatt, Dr.Roy. Application Report - AlbuVoid™LC-MS On-Bead – Differential Expression of Cancer Sera ProteinsUsing Quantitative (iTRAQ) Proteomics
Plasma
Espes,Daniel, Joey Lau, and Per-Ola Carlsson. "Increasedcirculating levels of betatrophin in individuals with long-standingtype 1 diabetes."Diabetologia(2013):1-4.
Patent- Secretome Sample Preparation
Narain,Niven Rajin, and Paula Patricia Narain. "COMPOSITIONSAND METHODS FOR DIAGNOSIS AND TREATMENT OF PERVASIVE DEVELOPMENTALDISORDER."U.S.Patent No. 20,150,023,949. 22 Jan. 2015.
Narain,Niven Rajin, Rangaprasad Sarangarajan, and Vivek K. Vishnudas." INTERROGATORYCELL-BASED ASSAYS AND USES THEREOF."U.S. Patent No. 20,120,258,874. 11 Oct. 2012.
CellCulture
Narain,Niven Rajin, Rangaprasad Sarangarajan, Vivek K. Vishnudas, andMichael Andrew Kiebish."USEOF MARKERS IN THE IDENTIFICATION OF CARDIOTOXIC AGENTS AND IN THEDIAGNOSIS AND MONITORING OF CARDIOMYOPATHY AND CARDIOVASCULARDISEASE."U.S. Patent 20,140,100,128, issued April 10, 2014.
On-BeadDigestion Protocols For LC-MS Proteomic Workflows
Newon-bead digestion for LC-MS applications for proteomic studies
USHUPO 2014. Frontiers in Proteomics: Advancing Biology throughTechnology and Computation.
AlbuVoid™ abstractentitled "Improvedproteomic enrichment and workflow strategies",posterboard 089 presented at US HUPO 2014
AlbuTrial™PLUS Kit
Tissue Lysates
Zakari, Abubakar, et al."Extreme Temperature Exposure Induces Lung-Gut Dysbiosis in Healthy Mice.", Current Research in Microbial Sciences (2026): 100564.
Red Cell Lysates
Wang, Y., Wu, X., Li, X., Liu, Z., An, N., Gu, S., He, Y., He, M., Yin, D., Chen, Y., Hu, X.,"Ubiquitination and degradation of CD47 enhances macrophage phagocytosis of hemolytic erythrocytes", iScience (2026), doi: https://doi.org/10.1016/j.isci.2025.114499 .
Tumor Interstitial Fluid
Zhang, X., Li, S., Malik, I. et al. "Reprogramming tumour-associated macrophages to outcompete cancer cells."Nature (2023).
Whole blood
Leitner, Dominique F., et al."Metabolomic, Proteomic, and Transcriptomic Changes in Adults with Epilepsy on Modified Atkins Diet."Epilepsia (2023).
Tissue Lysates
Zakari, Abubakar, et al. "Extreme Temperature Exposure Induces Lung-Gut Dysbiosis in Healthy Mice.", Current Research in Microbial Sciences (2026): 100564.
Red Cell Lysates
Wang, Y., Wu, X., Li, X., Liu, Z., An, N., Gu, S., He, Y., He, M., Yin, D., Chen, Y., Hu, X.,"Ubiquitination and degradation of CD47 enhances macrophage phagocytosis of hemolytic erythrocytes", iScience (2026), doi: https://doi.org/10.1016/j.isci.2025.114499 .
Red Cell Lysates
Go, Ka Diam, et al."Antimalarial drug artemisinin stabilizes PfRACK1 binding to the ribosome."Structure (2025).
Tissue Lysates, LC-MS Proteomics and Species Agnostic – Applications to Different Species
Liu, Yi-Hsuan, et al."ITIH4 alleviates OVA-induced asthma by regulating lung-gut microbiota."Molecular Medicine 31 (2025): 204
Red Blood Cells
Chen, Yaozhen, et al."Red blood cells undergo lytic programmed cell death involving NLRP3."Cell (2025).
Fresh frozen human EDTA-whole Blood
de Boni, Laura, et al. "Aggregation-resistant alpha-synuclein tetramers are reduced in the blood of Parkinson’s patients."EMBO Molecular Medicine (2024): 1-18.
EDTA Whole Blood (EWB)
Das, Amaresh, et al."Enhanced Recovery and Detection of Highly Infectious Animal Disease Viruses by Virus Capture Using Nanotrap® Microbiome a Particles."(2024).
Hemolyzed Samples
Neil Adrian P. Ondevilla, Peng-Wen Liu, Wan-Ting Huang, Tzu-Ping Weng, Nan-Yao Lee, Syu-Cing Ma, Jian-Jang Huang, Tak-Wah Wong, Hsien-Chang Chang,"A point-of-care electrochemical biosensor for the rapid and sensitive detection of biomarkers in murine models with LPS-induced sepsis"Biosensors and Bioelectronics, Volume 254, 2024, 116202, ISSN 0956-5663,
Pooled Blood Samples
Igoh, Akihisa, Masanobu Miura, and Satoru Miyaishi."Animal Species and Blood Identification with Peptide Mass Fingerprinting."Analytical Chemistry (2024).
RBC lysates
Chen, Yaozhen, et al."Noncanonical NLRP3 Inflammasome Activation Elicits the Programmed Death of Red Blood Cells."Available at SSRN 4626203.
Abdominal Aneurysm Samples
Jara, Zaira Palomino, et al."Distinct Mechanisms of β-Arrestin–Biased Agonist and Blocker of AT1R in Preventing Aortic Aneurysm and Associated Mortality."Hypertension 80.2 (2023): 385-402.
Peripheral blood mononuclear cell (PBMC) whole cell lysate samples
Kaneko, Tomonori, et al."Kinome and phosphoproteome reprogramming underlies the aberrant immune responses in critically ill COVID-19 patients."(2023).
Red blood cells
Barakat, Amey, et al."Effects of 2,3-DPG knockout on SCD phenotype in Townes SCD model mice"American Journal of Hematology (2023).
Mouse Red Blood Cells
Zhan, Jing, et al."Silica nanoparticles trigger phosphatidylserine exposure in red blood cells and induce thrombosis risk."Environmental Pollution (2023): 121591.
Hemolysate
Dasauni, Pushpanjali, et al."Optimization and Identification of Single Mutation in Hemoglobin Variants with 2, 2, 2 Trifluoroethanol Modified Digestion Method and Nano− LC Coupled MALDI MS/MS."Molecules 27.19 (2022): 6357.
Whole Blood Sample
Witchey, Shannah K., et al. ""Reproductive and developmental toxicity following exposure to organophosphate ester flame retardants and plasticizers, triphenyl phosphate and isopropylated phenyl phosphate, in Sprague Dawley rats.""Toxicological Sciences (2022).
Sae-Lee, Wisath, et al. " The protein organization of a red blood cell ." Cell Reports 40.3 (2022): 111103.
Plasma Samples
Phillippi, Danielle T., et al. "Inhaled diesel exhaust particles result in microbiome-related systemic inflammation and altered cardiovascular disease biomarkers in C57Bl/6 male mice." Particle and fibre toxicology 19.1 (2022): 1-29.
Human serum
High-throughput, Automated INTip™ SPE Combined with AlbuSorb™ Products for Efficient Albumin and Albumin/IgG Depletion Matthew Fitts 1; William E. Kemnitzer 1; Paul Meeh 1; Matt Kuruc 2; Swapan Roy 2; Sowmya Avadhani 21DPX Technologies, Columbia, SC; 2Biotech Support Group LLC, Monmouth Junction, NJ Corresponding Author: [email protected]
Juriasingani,Smriti, et al. "Evaluatingthe Effects of Subnormothermic Perfusion with AP39 in a NovelBlood-Free Model of Ex Vivo Kidney Preservation andReperfusion." InternationalJournal of Molecular Sciences 22.13(2021): 7180.
LysedRBCs
D'Alessandro,Angelo, et al. "Hematologicand systemic metabolic alterations due to Mediterranean type II G6PDdeficiency in a novel murine model." bioRxiv (2021).
HeatherE. McKiernan, Phillip B. Danielson, Catherine O. Brown, MashaSignaevsky, Christian G. Westring and Kevin M. Legg, DevelopmentalValidation of a Multiplex Proteomic Assay for the Identification ofForensically Relevant Biological Fluids, Forensic ScienceInternational, (2021)
Peripheral blood derived mononuclear cells whole cell lysate samples
Kaneko, Tomonori, et al."System-wide hematopoietic and immune signaling aberrations in COVID-19 revealed by deep proteome and phosphoproteome analysis."Research Square preprint (2021).
Blood cell lysates
Yamagishi, Yoshikazu, Hirotaro Iwase, and Yasumitsu Ogra."Post-Mortem Changes of Methomyl in Blood with Hemoglobin."Chemical Research in Toxicology.
Reticulocytes (Retics) lysates
Hojo-Souza NS, de Azevedo PO, de Castro JT, Teixeira-Carvalho A, Lieberman J, et al "Contributions of IFN-γ and granulysin to the clearance of Plasmodium yoelii blood stage."PLOS Pathogens 16(9): e1008840.
Blood
Southwell,Rebecca Marie, Kenneth Sherlock, and Matthew Baylis. "Cross-sectionalstudy of British wild deer for evidence of Schmallenberg virusinfection."Veterinary Record (2020).
ApplicationReports
HemoglobinRemoval Whitepaper Describes a Range of Depletion Strategies andTheir Successful Uses
“HemoglobinRemoval - The Gold Standard Products”,April 28, 2020
ebookDescribes the Role of the Innate Immunity Proteome As a CentralModulator of Disease from Pandemic Infections toCancer
“Categorizationof Blood Based Biomarkers – Unleashing the Power of ProteomicsTo Better Understand the Innate Immune Response to Infectious andNon-infectious Inflammatory Stimuli”,April 17, 2020
Hakuna,Lovemore, et al. " Asimple assay for glutathione in whole blood."Analyst (2015).
WholeBlood
Chalásová,Katarína, et al. "TransketolaseActivity but not Thiamine Membrane Transport Change in Response toHyperglycaemia and Kidney Dysfunction."Experimental and Clinical Endocrinology & Diabetes(2017).
Chalásová, Katarína. Functionalanalysis of genetic variants in genes associated with the developmentof glycotoxic damage.Diss. Masarykova univerzita, Lékařská fakulta,2017.
Lahut,Suna, et al. "BloodRNA biomarkers in prodromal PARK4 and REM sleep behavior disordershow role of complexin-1 loss for risk of Parkinson's disease."Disease Models & Mechanisms (2017): dmm-028035.
Brittain,Matthew K., Kevin G. McGarry, Robert A. Moyer, Michael C. Babin,David A. Jett, Gennady E. Platoff, and David T. Yeung. "Efficacyof Recommended Prehospital Human Equivalent Doses of Atropine andPralidoxime Against the Toxic Effects of Carbamate Poisoning in theHartley Guinea Pig." Internationaljournal of toxicology (2016): 1091581816638086.
Snider,Thomas H., Kevin G. McGarry, Michael C. Babin, David A. Jett, GennadyE. Platoff Jr, and David T. Yeung. "Acutetoxicity of phorate oxon by oral gavage in the Sprague-Dawleyrat." FundamentalToxicological Sciences 3, no. 5 (2016): 195-204.
Bhargava,Maneesh, Kevin J. Viken, Sanjoy Dey, Michael S. Steinbach, Baolin Wu,Pratik D. Jagtap, LeeAnn Higgins et al. "ProteomeProfiling in Lung Injury after Hematopoietic StemCell Transplantation." Biologyof Blood and Marrow Transplantation (2016).
Snider,Thomas H., Christina M. Wilhelm, Michael C. Babin, Gennady E. PlatoffJr, and David T. Yeung. "Assessingthe therapeutic efficacy of oxime therapies against percutaneousorganophosphorus pesticide and nerve agent challenges in the Hartleyguinea pig."TheJournal of Toxicological Sciences 40, no. 6 (2015): 759-775.
BloodPlasma
Johns,Michael, et al. "SR-135,a peroxynitrite decomposing catalyst, enhances β-cell functionand survival in B6D2F1 mice fed a high fat diet."Archivesof Biochemistry and Biophysics (2015).
Asleh,Rabea, et al. " HaptoglobinGenotype-dependent Differences in Macrophage Lysosomal OxidativeInjury." Journalof Biological Chemistry289.23(2014): 16313-16325.
BiologicalFluids
JKrupey - United States Patent: 10/180,053, 2002Removalof extraneous substances from biological fluids containing nucleicacids and the recovery of nucleic acids
LungTissue Specimens & Pulmonary Research (Acute Respiratory DistressSyndrome & Acute Hypoxic Failure)
Bhargava,Maneesh""ProteomicStudies in Acute Hypoxic Respiratory Failure." PhD diss.,UNIVERSITY OF MINNESOTA.(2015).
RedBlood Cell (RBC) - Lysates.
KyoungsookPark, Christopher D. Saudek, and Gerald W. HartIncreasedExpression of β-N-Acetylglucosamindase (O-GlcNAcase) inErythrocytes from Prediabetic and DiabeticIndividuals.Diabetes.2010;59(7):1845-50.
Barasa,Benjamin, and Monique Slijper. "Challengesfor red blood cell biomarker discovery throughproteomics." Biochimicaet Biophysica Acta (BBA)-Proteins and Proteomics 1844.5(2014): 1003-1010
StoredBlood Products
DelobelJ., Rubin O., Prudent M., Crettaz D., Tissot J.-D., LionN.(2010)BiomarkerAnalysis of Stored Blood Products: Emphasis on Pre-AnalyticalIssues. InternationalJournal of Molecular Sciences. 11(11):4601-4617
RedBlood Cells/Forensic Research
Danielson,Phillip B. "Isolationof Highly Specific Protein Markers for the Identification ofBiological Stains: Adapting Comparative Proteomics to Forensics."(2011).
RedBlood Cells (RBC) - Blood.
Wilhelm,Christina M., et al.Acomprehensive evaluation of the efficacy of leading oxime therapiesin guinea pigs exposed to organophosphorus chemical warfare agents orpesticides. Toxicologyand Applied Pharmacology Available online 31 October 2014.doi:10.1016/j.taap.2014.10.009
Hikosaka,Keisuke, et al. "Deficiencyof Nicotinamide Mononucleotide Adenylyltransferase 3 (Nmnat3) CausesHemolytic Anemia by Altering the Glycolytic Flow in MatureErythrocyte"Journalof Biological Chemistry(2014):jbc-M114.
McGarry,Kevin G., et al. " Evaluationof HemogloBind™ treatment for preparation of samples forcholinesterase analysis."(2013). Advances in Bioscience and Biotechnology, 2013, 4, 1020-1023
Alvarez-Llamas,Gloria, Fernando de la Cuesta, Maria G. Barderas, Irene Zubiri, MariaPosada-Ayala, and Fernando Vivanco. "Characterizationof Membrane and Cytosolic Proteins of Erythrocytes."In Vascular Proteomics, pp. 71-80. Humana Press,2013.
Alvarez-Llamas,G., de la Cuesta, F., Barderas, M. G., Darde, V. M., Zubiri, I.,Caramelo, C., Vivanco, F. Anovel methodology for the analysis of membrane and cytosolicsub-proteomes of erythrocytes by2-DE.Electrophoresis.2009;30:4095-4108
ZihaoWang, Kyoungsook Park, Frank Comer1, Linda C. Hsieh-Wilson,Christopher D. Saudek, Gerald W. Hart.Site-SpecificGlcNAcylation of Human Erythrocyte Proteins: Potential Biomarker(s)for Diabetes Mellitus. Diabetes.2008;58,309-317.
Datta,Pradip.Effectof Hemolysis, High Bilirubin, Lipemia, Paraproteins, and SystemFactors on Therapeutic Drug Monitoring. Handbookof Drug Monitoring Methods.2008; 97-109.
YuichiMiki, Tomoki Tazawa, Kazuya Hirano, Hideki Matsushima, ShokoKumamoto, Naotaka Hamasaki, Tomohiro Yamaguchi, MasatoshiBeppu.Clearanceof oxidized erythrocytes by macrophages: Involvement of caspases inthe generation of clearance signal at band 3glycoprotein. Biochemicaland Biophysical Research Communications.2007; 363(1):57-62
Sarawathi,etal.,Relativequantification of glycated Cu-Zn superoxide dismutase in erythrocytesby electrospray ionization mass spectrometry, Biochimicaet Biophysica Acta. 1999.1426(3):483-90
Bilirubin
Krishna,Neel K., and Kenji Cunnion. "DerivativePeptide Compounds and Methods of Use."U.S. Patent No. 20,160,376,322. 29 Dec. 2016.
Person,N.B., Effect Of HemogloBind™ On Interference Reduction InBilirubin Analysis.poster Clinichem, 1995.
Serum
Nishida-Aoki,Nao, et al. "Disruptionof Circulating Extracellular Vesicles as a Novel Therapeutic Strategyagainst Cancer Metastasis."Molecular Therapy 25.1 (2017): 181-191.
Baion, C.M. & Ali,A.C.EvaluationOf HemogloBind™ For Removal Of O-Raffinose CrosslinkedHemoglobin (Hemolink™) From Serum, posterAACC Meeting 1997.
Tissue
Padilla,S., Convenient Method for Decreasing the Amount of Hemoglobin inTissue Samples Without Affecting the Level of CholinesteraseActivity, unpublished personal correspondence, 1994.
Red Blood Cells (RBCs) / Other Applications
Mitra, Nibedita, et al. "Multi-omics analysis of red blood cells reveals thalassemia severity beyond globin gene mutations.", Blood Advances (2026): bloodadvances-2025016677.
Dried Blood Card/Spot Proteomics
Hannah Ging, Elin Davies, Claire E. Eyers, Louise Oni, Andrew Chetwynd."HAEMOGLOBIN INTERFERENCE IN DRIED BLOOD SPOT PROTEOMICS FOR CHRONIC KIDNEY DISEASE", Poster 2025 World Proteome Congress, Toronto Canada.
Red Blood Cells (RBCs) / Parkinson’s Disease / α-Synuclein
Parra M.A., Snyder M.P. "Parkinson’s Disease biomarker discovery via LC-MS/MS characterization and quantification of alpha synuclein PTMs in erythrocytes", Poster 2025 ASMS Conference, Baltimore MD.
Red Blood Cells (RBCs) / Other Applications
Xi-Long Wang, et al. (2025)"Proteome Profiling of Red Blood Cells from Patients with COPD Links Proteasome Activation with Abnormal Cell Morphology and Function"International Journal of Chronic Obstructive Pulmonary Disease, , 3279-3294, DOI: 10.2147/COPD.S531220
Red Blood Cell (RBC) lysates
Lan, Qin, and Zhao-bing Gu. "Data-independent acquisition-based proteome profiling of red blood cells from dairy buffaloes under different types of heat stress."Veterinary and Animal Science (2025): 100437.
RBC cells
Mitra, Nibedita, et al. "Multi-Omics Analysis of Red Blood Cells Reveals Molecular Pathways Underlying Thalassemia Severity Beyond Globin Gene Mutations."medRxiv (2025): 2025-02.
Pooled Blood Samples
Igoh, Akihisa, Masanobu Miura, and Satoru Miyaishi."Animal Species and Blood Identification with Peptide Mass Fingerprinting."Analytical Chemistry (2024).
Red Blood Cells
Cao, Chan, et al. "Deep learning-assisted single-molecule detection of protein post-translational modifications with a biological nanopore."bioRxiv (2023): 2023-09.
Erythrocyte Lysate Samples
Das, Sonu, et al."A journey to unravel the pathophysiology of stable and exacerbated Chronic obstructive pulmonary disease through erythrocyte proteomics: A combined mass spectrometry/bioinformatics approach."(2022).
Blood Cell Proteins
Wu, Na, et al. "Proteomic characteristics of plasma and blood cells in natural aging rhesus monkeys."Proteomics: 2200049.
Jak2VF Red Blood Cells (RBC)
Liu, Wenli, et al."Erythroid lineage Jak2 V617F expression promotes atherosclerosis through erythrophagocytosis and macrophage ferroptosis."The Journal of Clinical Investigation (2022).
Fasting Blood Samples
Wang, Jijun, et al."The D2R-DISC1 protein complex and associated proteins are altered in schizophrenia and normalized with antipsychotic treatment."Journal of Psychiatry and Neuroscience 47.2 (2022): E134-E147.
Blood
Pawliński,Łukasz, et al. "Proteomicbiomarkers in Gaucher disease." Journalof clinical pathology 74.1(2021): 25-29.
Jing, Lun, et al. "PROTEOMIC ANALYSIS IDENTIFIED LBP AND CD14 AS KEY PROTEINS IN BLOOD/BIPHASIC CALCIUM PHOSPHATE MICROPARTICLE INTERACTIONS." Acta Biomaterialia, (2021).
RBC samples
Christer, M. A. L. M., et al.""Methods for the detection of autologous blood-doping.""U.S. Patent Application No. 16/976,936.
Blood
Pawliński,Łukasz, et al."Proteomicbiomarkers in Gaucher disease."Journalof Clinical Pathology (2020).
ApplicationReports
HemoglobinRemoval Whitepaper Describes a Range of Depletion Strategies andTheir Successful Uses
“HemoglobinRemoval - The Gold Standard Products”,April 28, 2020
ebookDescribes the Role of the Innate Immunity Proteome As a CentralModulator of Disease from Pandemic Infections toCancer
“Categorizationof Blood Based Biomarkers – Unleashing the Power of ProteomicsTo Better Understand the Innate Immune Response to Infectious andNon-infectious Inflammatory Stimuli”,April 17, 2020
Erythrocytes
Bollenbach, Alexander, et al.."GC-MS and LC-MS/MS pilot studies on the guanidine (NG)-dimethylation in native, asymmetrically and symmetrically NG-dimethylated arginine-vasopressin peptides and proteins in human red blood cells"." Journal of Chromatography B (2020): 122024
Erythrocytes
Klatt, Stephan, et al."Optimizing red blood cell protein extraction for biomarker quantitation with mass spectrometry."." Analytical and Bioanalytical Chemistry (2020): 1-14
Erythrocytes
Kitao, Akihito, et al."Band 3 ectopic expression in colorectal cancer induces an increase in erythrocyte membrane-bound IgG and may cause immune-related anemia."." International Journal of Hematology (2020): 1-10.
Haemolysates
Rosin-Arbesfeld, Rina, and Ronen SIMAN-TOV. "Article of manufacture and methods for increasing survival of red blood cells." ." U.S. Patent Application No. 15/739,857.
RainbowTrout Fish Nucleated Red Blood Cells (RBCs)
Puente-Marin,Sara, Iván Nombela, Sergio Ciordia, María Carmen Mena,Verónica Chico, Julio Coll, and María del MarOrtega-Villaizan. "InSilico Functional Networks Identified in Fish Nucleated Red BloodCells by Means of Transcriptomic and Proteomic Profiling."Genes 9, no. 4 (2018): 202.
NombelaI, Puente-Marin S, Chico V et al.""Identificationof diverse defense mechanisms in trout red blood cells in response toVHSV halted viral replication [version 1; referees: awaiting peerreview]."."F1000Research 2017, 6:1958 (doi: 10.12688/f1000research.12985.1)
Nombela,Ivan, Sara Puente-Marin, Veronica Chico, Alberto J. Villena, BegoñaCarracedo, Sergio Ciordia, Maria Carmen Mena et al. "troutred blood cells in response to halted replication of VHS virus[version 2; referees: 3 approved with reservations]."
Rogers,Stephen C., and Allan Doctor. "Hypoxiamodulates the purine salvage pathway and decreases red blood cell andsupernatant levels of hypoxanthine during refrigerated storage."(2018).
Cortese-Krott,Miriam M., et al.""Identificationof a soluble guanylate cyclase in RBCs: preserved activity inpatients with coronary artery disease."."Redox Biology (2017).
Carvalho,Ana Sofia, Manuel S. Rodriguez, and Rune Matthiesen. "RedBlood Cells in Clinical Proteomics."Serum/Plasma Proteomics: Methods and Protocols (2017): 173-181.
HumanRed Blood Cells (RBC)
Nemkov,Travis, Kaiqi Sun, Julie A. Reisz, Anren Song, Tatsuro Yoshida,Andrew Dunham, Matthew J. Wither et al. "Hypoxiamodulates the purine salvage pathway and decreases red blood cell andsupernatant levels of hypoxanthine during refrigerated storage."haematologica 103, no. 2 (2018): 361-372.
Feliciano,Amélia, Fátima Vaz, Cristina Valentim-Coelho, VukosavaM. Torres, Rita Silva, Vesna Prosinecki, Bruno M. Alexandre et al."Eveningand morning alterations in Obstructive Sleep Apnea red blood cellproteome."Data in Brief (2017).
HemoVoid™On Bead Digestion Application Work On RBC byIrene Granlund, UmeåUniversity
RedBlood Cells, Plasmodium extracts
Machado,Patrícia Isabel Pires. Pyruvatekinase and glucose-6-phosphate dehydrogenase deficiencies and theirassociation with malaria–population genetics and proteomicstudies. Diss.Universidade do Porto, 2013.
Walpurgis,Katja, et al. " Effectsof gamma irradiation and 15 days of subsequent ex vivo storage on thecytosolic red blood cell proteome analyzed by 2D DIGE and OrbitrapMS." PROTEOMICS-ClinicalApplications (2013).
P.Falciparum Clone 3D7 Cultured In Human Erythrocytes
LasonderE, Green JL, Camarda G, Talabani H, Holder AA, Langsley G, AlanoP. ThePlasmodium falciparum schizont phospho-proteome reveals extensivephosphatidylinositol and cAMP-Protein Kinase A signalling. JProteome Research. 2012;
RedBlood Cell Lysate
Barasa,Benjamin, and Monique Slijper. "Challengesfor red blood cell biomarker discovery throughproteomics." Biochimicaet Biophysica Acta (BBA)-Proteins and Proteomics 1844.5(2014): 1003-1010.
Lange, Philipp F., Pitter F. Huesgen, KarenNguyen, and Christopher M. Overall. "AnnotatingN termini for the Human Proteome Project: N termini andNα-acetylation status differentiate stable cleaved proteinspecies from degradation remnants in the human erythrocyteproteome."Journalof proteome research (2014).
KatjaWalpurgis, Maxie Kohler, Andreas Thomas et al.Validatedhemoglobin-depletion approach for red blood cell lysate proteomeanalysis by means of 2D-PAGE and Orbitrap MS.Electrophoresis.2012;
Mizukawa,B., George, A., Pushkaran, S. et al. CooperatingG6PD mutations associated with severe neonatal hyperbilirubinemia andcholestasis.PediatricBlood Cancer.2011;56: 840-842.
SudhaNeelam, David G Kakhniashvili, Stephan Wilkens et al. Functional20S proteasomes in mature human red blood cells ExperimentalBiology and Medicine.2011;236:580-591
HemoVoid™Blood Card Kit
The HemoVoid™ Blood Card kitsubstantially reduces hemoglobin interference from dried blood spotprotein analytes
Red Blood Cells (RBCs) / Other Applications
Mitra, Nibedita, et al."Multi-omics analysis of red blood cells reveals thalassemia severity beyond globin gene mutations."Blood Advances (2026): bloodadvances-2025016677.
Dried Blood Card/Spot Proteomics
Hannah Ging, Elin Davies, Claire E. Eyers, Louise Oni, Andrew Chetwynd."HAEMOGLOBIN INTERFERENCE IN DRIED BLOOD SPOT PROTEOMICS FOR CHRONIC KIDNEY DISEASE", Poster 2025 World Proteome Congress, Toronto Canada.
RedBlood Cells
Driedblood spots for detection of autologous blood doping with targetedLC-MS/MS
MattKuruc1; Swapan Roy1; Johan Jakobsson2; Raik Wagner2; ChristerMalm2; 1Biotech Support Group (BSG) LLC, Monmouth Junction,NJ; 2Pro Test Diagnostics AB (PTD), Umeå, Sweden
HemoVoid™ LC-MS On-Bead For RBC Proteomics
Red Blood Cells (RBCs) / Other Applications
Mitra, Nibedita, et al. "Multi-omics analysis of red blood cells reveals thalassemia severity beyond globin gene mutations.", Blood Advances (2026): bloodadvances-2025016677.
Dried Blood Card/Spot Proteomics
Hannah Ging, Elin Davies, Claire E. Eyers, Louise Oni, Andrew Chetwynd."HAEMOGLOBIN INTERFERENCE IN DRIED BLOOD SPOT PROTEOMICS FOR CHRONIC KIDNEY DISEASE", Poster 2025 World Proteome Congress, Toronto Canada.
HemogloBind™Blood Card Kit
Hemoglobin Depletion and ProteinEnrichment From Dried Blood Spots
RedBlood Cells
Driedblood spots for detection of autologous blood doping with targetedLC-MS/MS
MattKuruc1; Swapan Roy1; Johan Jakobsson2; Raik Wagner2; ChristerMalm2; 1Biotech Support Group (BSG) LLC, Monmouth Junction,NJ; 2Pro Test Diagnostics AB (PTD), Umeå, Sweden
Tissue Lysates
Zakari, Abubakar, et al. "Extreme Temperature Exposure Induces Lung-Gut Dysbiosis in Healthy Mice.", Current Research in Microbial Sciences (2026): 100564.
Red Cell Lysates
Wang, Y., Wu, X., Li, X., Liu, Z., An, N., Gu, S., He, Y., He, M., Yin, D., Chen, Y., Hu, X.,"Ubiquitination and degradation of CD47 enhances macrophage phagocytosis of hemolytic erythrocytes", iScience (2026), doi: https://doi.org/10.1016/j.isci.2025.114499 .
Tissue Lysates
Zakari, Abubakar, et al. "Extreme Temperature Exposure Induces Lung-Gut Dysbiosis in Healthy Mice.", Current Research in Microbial Sciences (2026): 100564.
Red Cell Lysates
Wang, Y., Wu, X., Li, X., Liu, Z., An, N., Gu, S., He, Y., He, M., Yin, D., Chen, Y., Hu, X.,"Ubiquitination and degradation of CD47 enhances macrophage phagocytosis of hemolytic erythrocytes", iScience (2026), doi: https://doi.org/10.1016/j.isci.2025.114499 .
Cell Response
Suarez-Carmona, Meggy, et al. "Harnessing lipid-driven immunometabolic pathways in omental metastases to enhance immunotherapy in patients with ovarian cancer."Signal Transduction and Targeted Therapy 11.1 (2026): 78.
Cell Response
Toraason, Erik, et al. "Caenorhabditis elegans avoids Todstoff, a novel nociceptive necrotaxis cue"bioRxiv (2026): 2026-02.
Plasma/Serum
Post-Stroke Immune Suppression is Mediated by Bioactive Lipids”,An abstract describes the use of Cleanascite™ to compare stroke plasma, in ex vivo models of immune suppression.
Tissue Proteomics
Guldner, Ian H., et al. "Ageing promotes microglial accumulation of slow-degrading synaptic proteins."Nature (2026): 1-12.
Tumor microenvironment (TME)
Dyer A., Dudley R., Ahuja S. et al. "Characterisation of human in vitro tumour-associated macrophage models to define translational relevance."Sci Rep (2025).
Plasma/Serum
Zhao, Yuan, et al. "A Label-Free Nanopore Biosensor for Rapid and Highly Sensitive Detection of Actinomycin in Human Serum."ACS Measurement Science Au (2025).
Tumor microenvironment (TME)
Dalangood, Sumiya et al. "Cancer-associated adipocytes mediate CD8 + T cell dysfunction via FGF21-driven lipolysis"Cell Reports, Volume 44, Issue 11, 116526.
Tissue Proteomics
Thomas, R., et al. "Toxicological Profile of Chemical Interference of Thyroid Hormone Metabolism via Deiodinase Enzymes In a Rodent Model."Toxicological Sciences (2025): kfaf141.
Tissue Proteomics
Guldner, Ian H., et al. "Synaptic proteins that aggregate and degrade slower with aging accumulate in microglia."bioRxiv (2025): 2025-05.
Tumor microenvironment (TME)
Curtin, Meghan C., et al. "Lean breast adipocytes secrete an oxylipin that suppresses breast cancer via ferroptosis."bioRxiv (2025): 2025-07.
Tumor microenvironment (TME)
Chu, Tianhao, et al. "Metabolism archetype cancer cells induce protumor TREM2+ macrophages via oxLDL-mediated metabolic interplay in hepatocellular carcinoma."Nature Communications 16.1 (2025): 6770.
Tumor cell-conditioned media (TCM)
Li, Xiaoyun, et al. "Tumor-instructed glutamine synthesis in cancer-associated fibroblasts promotes pro-tumor macrophages."Journal of Experimental Medicine 222.9 (2025): e20241426.
Fetal Calf Serum (FCS) and Ascites Samples
Slattery, Karen, et al. ""Uptake of lipids from ascites drives NK cell metabolic dysfunction in ovarian cancer." "Science Immunology 10.107 (2025): eadr4795.10.1016/j.ccell.2025.04.00
Secretomes
Gurung et al.,"Stromal lipid species dictate melanoma metastasis and tropism,"Cancer Cell (2025), https://doi.org/ 10.1016/j.ccell.2025.04.00
Conditioned Media (CM)
Huo, Yazhen, et al."Ceramide mediates cell-to-cell ER stress transmission by modulating membrane fluidity."Journal of Cell Biology 224.5 (2025): e202405060.
Mouse Serum
Chen, Chao, et al."Obesity-driven oleoylcarnitine accumulation in tumor microenvironment promotes breast cancer metastasis-like phenotype."Acta Pharmaceutica Sinica B (2025).
Conditioned media
Flury, Anna, et al."A neurodegenerative cellular stress response linked to dark microglia and toxic lipid secretion."Neuron (2024).
Phospholipid analysis
Playford M, Li H, Dey A, Florida E, Teague H, Gordon S, Mehta N,"HDL associated Vitamin D Binding Protein levels are Inversely Associated with Necrotic Plaque Burden in Psoriasis",Atherosclerosis Plus, https://doi.org/10.1016/j.athplu.2024.12.002.
Serum Samples
Huneault HE, Lo JS, Bai S, He Z, McPhaul MJ, Bril F, et al."Fasting intact insulin by mass spectrometry is associated with metabolic dysfunction-associated steatotic liver disease in youth." Hepatol Commun. 2024;8: e0582. https://doi.org/10.1097/ HC9.0000000000000582
Cancer Supernatant Culture Media
Dahal, Ankit, et al. "Platelet-activating factor (PAF) promotes immunosuppressive neutrophil differentiation within tumors." Proceedings of the National Academy of Sciences 121.35 (2024): e2406748121.
Omental Conditioned Medium (OCM)
Wang, H., Yung, M.M., Xuan, Y. et al. "Polyunsaturated fatty acids promote M2-like TAM deposition via dampening RhoA-YAP1 signaling in the ovarian cancer microenvironment." Exp Hematol Oncol 13, 90 (2024). https://doi.org/10.1186/s40164-024-00558-8
Human saliva samples
Arora, Pearl, et al."Nanopore”based detection of periodontitis biomarker miR31 in saliva samples." Electrophoresis (2024).
MRS+CQ broth (De Man, Rogosa, and Sharpe broth with cysteine and glutamine)
Zhu, Meilin, et al."Vaginal Lactobacillus fatty acid response mechanisms reveal a metabolite-targeted strategy for bacterial vaginosis treatment." Cell (2024).
Toxoplasma gondii
Swapna LS, Stevens GC, Sardinha-Silva A, Hu LZ, Brand V, Fusca DD, et al."ToxoNet: A high confidence map of protein-protein interactions in Toxoplasma gondii." PLoS Comput Biol 20(6): e1012208. https://doi.org/10.1371/ journal.pcbi.1012208
Conditioned Media (CM)
Zhang, T., Zhao, F., Hu, Y. et al."Environmental monobutyl phthalate exposure promotes liver cancer via reprogrammed cholesterol metabolism and activation of the IRE1α-XBP1s pathway." Oncogene (2024). https://doi.org/10.1038/s41388-024-03086-1
Flury, Anna, et al."Integrated stress response associated with dark microglia promotes microglial lipogenesis and contributes to neurodegeneration." bioRxiv (2024): 2024-03.
Adipocyte-conditioned media (ACM)
El-Hattab, Mariam Y., et al. "Native adiponectin plays a role in the adipocyte-mediated conversion of fibroblasts to myofibroblasts." Journal of the Royal Society interface 20.202 (2023): 20230004.
Ascites Fluid
Testa, Anna, et al. "TARGETING THE αVβ3/NgR2 PATHWAY IN NEUROENDOCRINE PROSTATE CANCER." Matrix Biology (2023).
Bovine Aorta
Yao, Yimin, et al. "Accurate quantification of lysyl oxidase concentration in human tissue."bioRxiv (2023): 2023-07.
PPACK PFC Nanoparticles
Vargas, Ian, et al. "Simultaneous Inhibition of Thrombosis and Inflammation Is Beneficial in Treating Acute Myocardial Infarction."International journal of molecular sciences 24.8 (2023): 7333.
Bone Marrow Samples
Gudgeon, Nancy, et al. "Uptake of long-chain fatty acids from the bone marrow suppresses CD8+ T-cell metabolism and function in multiple myeloma."Blood Advances (2023).
Activated hepatic stellate cells
Sinha, S., Aizawa, S., Nakano, Y. et al.Hepatic stellate cell stearoyl co-A desaturase activates leukotriene B4 receptor 2 - β-catenin cascade to promote liver tumorigenesis.Nat Commun 14, 2651 (2023). https://doi.org/10.1038/s41467-023-38406-8
Bile
Qin WH, Liu JT, Wang SP, Yang ZS, Wang KK, Hu B.Antibody array-based proteomic screening of novel biomarkers in malignant biliary stricture.Cancer Biomark. 2022;33(3):359-368. doi: 10.3233/CBM-210095. PMID: 34542063.
Bone Marrow (BM) Plasma
Gudgeon, Bishop, et al."CD8+ T cell metabolism and function are suppressed by long-chain fatty acid uptake from the bone marrow microenvironment in Multiple Myeloma."(2023).
Lacticaseibacillus casei BL23
da Silva Barreira, David, et al. "Membrane vesicles released by Lacticaseibacillus casei BL23 inhibit the biofilm formation of Salmonella Enteritidis."Scientific Reports 13.1 (2023): 1163.
RNA cargo
Castleberry, Mark, et al."High-density lipoproteins mediate small RNA intercellular communication between dendritic cells and macrophages."Journal of Lipid Research(2023): 100328.
Omental Conditioned Medium (OCM)
Chan, David, et al."Polyunsaturated Fatty Acids Promote Protumoral Macrophage Polarization via a RhoA-YAP1 Signaling Pathway in the Ovarian Cancer Microenvironment."Research Square (2022).
Bone Marrow Neutrophil Cells With RPMI Containing 0.5% FBS or with 10% FBS
Pham, Ly, et al."Neutrophil trafficking to the site of infection requires Cpt1a-dependent fatty acid β-oxidation."Communications Biology5.1 (2022): 1-13.
Cell Culture Grade Fetal Bovine Serum (FBS) and BSA
Jiang, Lifeng, et al." Akt-Dependent Glycolysis-Driven Lipogenesis Supports Proliferation and Survival of Human Pulmonary Arterial Smooth Muscle Cells in Pulmonary Hypertension ."Frontiers in medicine 9 (2022).
Detect Tuberculosis Virulence Factors In Serum
Zheng, Wenshu, et al. "Nanopore-based disease diagnosis using pathogen-derived tryptic peptides from serum."Nano Today 45 (2022): 101515.
Tumor-conditioned medium
Albakri, Marwah M., et al. "Fatty acids secreted from head and neck cancer induce M2”like Macrophages."Journal of Leukocyte Biology (2022).
SZ95 medium
Flori, E., Mastrofrancesco, A., Mosca, S., Ottaviani, M., Briganti, S., Cardinali, G., Filoni, A., Cameli, N., Zaccarini, M., Zouboulis, C.C., Picardo, M. ,Sebocytes contribute to melasma onset, ISCIENCE (2022).
Omental Conditioned Medium (OCM)
Yang, X. U. A. N., et al. "" SCD1/FADS2 fatty acid desaturases equipoise lipid metabolic activity and redox-driven ferroptosis in ascites-derived ovarian cancer cells ."" (2021).
Culture media
Di Conza, Giusy, et al. ""Tumor-induced reshuffling of lipid composition on the endoplasmic reticulum membrane sustains macrophage survival and pro-tumorigenic activity." " Nature immunology(2021): 1-13.
A precipitate of lipids
Greenfield, Edward A. ""Inducing, collecting, and storing ascites."" Cold Spring Harbor Protocols2021.10 (2021): pdb-prot103309.
Omental Conditioned Medium (OCM)
Wang, Xueyu, et al. ""Epigenetic Silencing of miR-33b Promotes Peritoneal Metastases of Ovarian Cancer by Modulating the TAK1/FASN/CPT1A/NF-κB Axis." " Cancers13.19 (2021): 4795.
Lipid-depletedserum
Chen,Hsin-Yi, et al. "Selectiveabrogation of S6K2 maps lipid homeostasis as a survival vulnerabilityin MAPKi-resistant NRASmut melanoma." bioRxiv(2021).
Birchpollen extracts
Pointner,Lisa, et al. "Birchpollen induces Toll-like receptor 4-dependent dendritic cellactivation favoring T cell responses."Frontiersin Allergy(2021):42.
Fetal Bovine Serum
Guyon, Léna, Miller-Rhodes, Patrick, and Harris A. Gelbard."The Cell Culture Environment Regulates the Transcription Factor MafB in BV-2 Microglia." Matters 7.1 (2021): e202010000001.
Predialysis PPACK-liposomes
Guyon, Léna, Anne-Claire Groo, and Aure”lie Malzert-Fre”on."Relevant Physicochemical Methods to Functionalize, Purify, and Characterize Surface-Decorated Lipid-Based Nanocarriers." Molecular Pharmaceutics (2020).
Egg Extract
Stahl, Elia, et al."Phosphatidylcholines from Pieris brassicae eggs activate an immune response in Arabidopsis." eLife 9 (2020): e60293.
Tissue culture supernatants
Greenfield, Edward A."Collecting and Storing Hybridoma Tissue Culture Supernatants."Cold Spring Harbor Protocols 2020.10 (2020): pdb-prot103317.
OCM (Omental conditioned medium) culture
Chan, David W., et al"MAP30 protein from Momordica charantia is therapeutic and has synergic activity with cisplatin against ovarian cancer in vivo by altering metabolism and inducing ferroptosis." Pharmacological Research (2020): 105157
Cancer cell-conditioned medium
Wang, Haiping, et al."CD36-mediated metabolic adaptation supports regulatory T cell survival and function in tumors." Nature Immunology (2020): 1-11
Omental conditioned medium (OCM)
Chen, Rain R., et al. "Targeting of lipid metabolism with a metabolic inhibitor cocktail eradicates peritoneal metastases in ovarian cancer cells." Communications Biology 2 (2019).
Human serum, mouse serum or DMEM
Lee, Hong-Jai, et al. "Regulatory effect of humoral milieu on the viral DNA and surface antigen expression of hepatitis B virus (HBV) in vitro. Molecular & Cellular Toxicology. 15.2 (2019): 123-128..
Body fluids
Farina, Annarita. "Pre-fractionation of Noncirculating Biological Fluids to Improve Discovery of Clinically Relevant Protein Biomarkers." Proteomics for Biomarker Discovery. Humana Press, New York, NY, 2019. 23-37.
Astrocyte conditioned media
Sprenkle, Neil T., et al. "Endoplasmic reticulum stress is transmissible in vitro between cells of the central nervous system." Journal of Neurochemistry.
Whole Mouse Serum
Dean, E. Danielle, et al. "Interrupted glucagon signaling reveals hepatic α cell axis and role for L-glutamine in α cell proliferation." Cell metabolism 25.6 (2017): 1362-1373.
Plasma
Swertfeger, Debi K., et al Feasibility of a Plasma Bioassay to Assess Oxidative Protection of Low Density Lipoproteins by High Density Lipoproteins" Journal of Clinical Lipidology (2018).
Estrous sheep serum
Barrera, N., dos Santos Neto, P. C., Cuadro, F., Bosolasco, D., Mulet, A. P., Crispo, M., & Menchaca, A. (2018). Impact of delipidated estrous sheep serum supplementation on in vitro maturation, cryotolerance and endoplasmic reticulum stress gene expression of sheep oocytesPloS one, 13(6), e0198742.
Extracellularvesicles (EVs) from bone marrow (BM)-derived mesenchymal stromalcells (BM-MSC)
Nguyen,Doan C., Holly C. Lewis, Chester Joyner, Vivien Warren, Haopeng Xiao,Haydn T. Kissick, Ronghu Wu, Jacques Galipeau, and F. Eun-Hyung Lee."Extracellularvesicles from bone marrow-derived mesenchymal stromal cells supportex vivo survival of human antibody secreting cells."Journal of extracellular vesicles 7, no. 1 (2018): 1463778.
PancreaticRough Endoplasmic Reticulum (RER) Fractions
Waldron,Richard T., Hsin-Yuan Su, Honit Piplani, Joseph Capri, Julian P.Whitelegge, Kym F. Faull, Sugunadevi Sakkiah et al. "EthanolInduced Disordering of Pancreatic Acinar Cell Endoplasmic Reticulum:An ER Stress/Defective Unfolded Protein Response Model."Cellular and Molecular Gastroenterology and Hepatology 5, no. 4(2018): 479-497
HumanSZ95 sebocytes
Lovászi,M., et al. "Sebumlipids influence macrophage polarization and activation."British Journal of Dermatology (2017). doi: 10.1111/bjd.15754
AscitesFluid
Chan,DW, Mak, SL, Ngan, HYS. The significance of lipid metabolism inperitoneal metastases of ovarian cancer. The 2016 Cold Spring HarbourAsia Conference on Cancer and Metabolism, Suzhou, China, 19-23September2016.http://hub.hku.hk/handle/10722/235385
Serum
Shiffman, Dov, et al. "Methods for quantitation of insulin and c-peptide." U.S. Patent Application No. 15/942,188
Taylor,Steven W., et al. "Ahigh-throughput mass spectrometry assay to simultaneously measureintact insulin and C-peptide."Clinica Chimica Acta (2016).
Serumor Blood
Berggren,Per Olof, and Shao-Nian Yang. "Methodsfor treating and/or limiting development of diabetes."U.S. Patent Application No. 14/995,341.
Bile
Vesterhus, Mette, et al."Novel serum and bile protein markers predict primary sclerosing cholangitis disease severity and prognosis." Journal of hepatology 66.6 (2017): 1214-1222.
Hauser-Davis, Rachel Ann. "Fish Bile Clean-up for Subsequent Zymography and Mass Spectrometry Proteomic Analyses." (2018).
Laohaviroj,Marut, et al. "Acomparative proteomic analysis of bile for biomarkers ofcholangiocarcinoma."Tumor Biology 39.6 (2017): 1010428317705764.
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Megger,Dominik A., et al. "OneSample, One Shot-Evaluation of sample preparation protocols for themass spectrometric proteome analysis of human bile fluid withoutextensive fractionation."Journal of Proteomics (2016).
Lukic, Natalija, et al." Anintegrated approach for comparative proteomic analysis of human bilereveals overexpressed cancer-associated proteins in malignant biliarystenosis." Biochimicaet Biophysica Acta (BBA)-Proteins and Proteomics1844.5(2014): 1026-1033.
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ChenB, Dong JQ, Chen YJ et al Two-dimensionalelectrophoresis for comparative proteomic analysis of humanbile. Hepatobiliary& pancreatic diseases international.2007 Aug;6(4):402-6
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KristiansenTZ, Bunkenborg J, Gronborg M et al AProteomic Analysis of Human Bile Molecularand Cellular Proteomics.2004;3:715-728
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LiD, Wang J, Wang R, Li Y. Ananobeads amplified QCM immunosensor for the detection of avianinfluenza virus H5N1,Biosensors and Bioelectronics.2011;26(S10):4146-4154
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Ahmed,Syed Mukhtar, and Ian G. Macara ThePar3 polarity protein is an exocyst receptor essential for mammarycell survival.. NatureCommunications.8 (2017): 14867. doi:10.1038/ncomms14867
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McIntyre,John A., et al. "Antiphospholipidautoantibodies as blood biomarkers for detection of early stageAlzheimer's disease." Autoimmunity0(2015): 1-8.
Knight,Jason S., Wei Luo, Alexander A. O'Dell, Srilakshmi Yalavarthi, WenpuZhao, Venkataraman Subramanian, Chiao Guo et al. " PeptidylarginineDeiminase Inhibition Reduces Vascular Damage and Modulates InnateImmune Responses in Murine Models of Atherosclerosis." Circulationresearch (2014):CIRCRESAHA-113.
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LijowskiM, Caruthers S, Hu G. High-ResolutionSPECT-CT/MR Molecular Imaging of Angiogenesis in the Vx2Model.InvestigativeRadiology.2009;44(1): 15–22
TurnerJD, Langley RS, Johnston KL. WolbachiaLipoprotein Stimulates Innate and Adaptive Immunity through Toll-likeReceptors 2 and 6 to Induce Disease Manifestations of FilariasisTheJournal of Biological Chemistry.2009;284:22364-22378
TorrellesJB, DesJardin LE, MacNeil J. et alInactivationof Mycobacterium tuberculosis mannosyltransferase pimB reduces thecell wall lipoarabinomannan and lipomannan content and increases therate of bacterial-induced human macrophage celldeathGlycobiology.2009;19(7):743-755
ChoN, Chueh PJ, Kim C et al Monoclonalantibody to a cancer-specific and drug-responsive hydroquinone (NADH)oxidase from the sera of cancer patients.CancerImmunology, Immunotherapy. 2002;51(3):121-9
ShapiroS, Beenhouwer DO, Feldmesser M et al.ImmunoglobulinG Monoclonal Antibodies to Cryptococcus neoformans Protect MiceDeficient in Complement Component C3 Infect. Infectionand immunity.2002;70(5):2598-604
CastroAR, Morrill We, Pope V. LipidRemoval from Human Serum SamplesClinicaland diagnostic laboratory immunology.2000;7(2):197-199
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Nanoparticles
Human& Plasma Cell Lines
Pan, Dipanjan, et al. "Astrategy for combating melanoma with oncogenic c-Myc inhibitors andtargeted nanotherapy." Nanomedicine 10.2(2015): 241-251.
Plasma-Enzyme-NanoparticleMixture
Pan,Dipanjan, et al. "Anti-AngiogenesisTherapy in the Vx2 Rabbit Cancer Model with a Lipase-cleavable Sn 2Taxane Phospholipid Prodrug using αvβ3-TargetedTheranostic Nanoparticles." Theranostics 2014;4(6):565-578
Microarray
Srinivasan,Harish."Antibodymicroarray as a proteomic tool for effective diagnosis and predictionof prognosis in cancer."(2014).
Saliva
LucyE. DesJardin Isolationof M. tuberculosis RNA from SputumMethodsin Molecular Medicine.2001;48:133-139
BeenhouwerDO, Shapiro S, Feldmesser M et al.BothTh1 and Th2 Cytokines Affect the Ability of Monoclonal Antibodies ToProtect Mice against Cryptococcus neoformansInfectionand immunity.2001;69: 6445-6455
DesjardinLE, Perkins MD, Wolski K et al. Measurementof Sputum Mycobacterium tuberculosis Messenger RNA as a Surrogate forResponse to Chemotherapy Americanjournal of respiratory and critical care medicine.1999;160(1):203-10
TemplatePreparation ProductionSequencing Protocols. Stanford Genome Technology Center
Patents
IwakuraYoichiro, Kakuta Shigeru, Suzuki, Shunsuke - United States PatentApplication 20130011413.Methodand Pharmaceutical Composition for Treatment of Intestinal Disease
Mcintyre,John A. United States Patent: 20120107841. SerumDiagnostic Method, Biomarker and Kit for Early Detection and Stagingof Alzheimer's Disease
Morre,James D et al. United States Patent: 20030170757. Monoclonalantibodies specific for neoplasia-specific NADH: disulfide reductase
DJMorre, NM McCarty, D Morre et al United States Patent:7053188. Monoclonalantibodies specific for neoplasia-specific NADH: disulfide reductase
DavidC. Jones. United States Patent: 7999084. Devicesand methods for reducing matrix effects
JKrupey - United States Patent: 5885921Hydrophobicsilica adsorbents for lipids
VaccineResearch (Cholesterol Removal From Human Serum)
Kamtchoua,Thierry, Monica Bologa, Robert Hopfer, David Neveu, Branda Hu,Xiaohua Sheng, Nicolas Corde, Catherine Pouzet, Gloria Zimmerman, andSanjay Gurunathan. Safetyand immunogenicity of the pneumococcal pneumolysin derivative PlyD1in a single-antigen protein vaccine candidate in adults.Vaccine(2012).
Yeastassays
Lifang,et al. Balancedglobin protein expression and heme biosynthesis improve production ofhuman hemoglobin in Saccharomyces cerevisiae. MetabolicEngineering (2013).
Lipoproteins
Turner,Joseph D., R. Stuart Langley, Kelly L. Johnston, Katrin Gentil,Louise Ford, Bo Wu, Maia Graham et al. "Wolbachialipoprotein stimulates innate and adaptive immunity through Toll-likereceptors 2 and 6 to induce disease manifestations of filariasis."Journal of Biological Chemistry 284, no. 33 (2009): 22364-22378.
Patent
Bhogal,John, Shridhara Alva Karinka, Timothy P. Henning, David Cunningham,Udo Hoss, Andrew H. Naegeli, and John Latour. "Methodsof Collecting and Analyzing Samples."U.S. Patent 20,120,296,189, issued November 22, 2012.
Review
ProCipitate™,Superior Substitute to Phenol/Chloroform for DNA Isolation &Protein Extraction.by Matt Kuruc February 23, 2015
Patents
U.S.Patent Numbers 5,294,681, 5,453,493 and other patents pending.
U.S.Patent Number 5,538,870,Methodfor Preparing Nucleic Acids For Analysis And Kits Useful Therefore.
EntericViruses
Richards,Gary P., and Gail E. Greening. "Detectionof enteric viruses in shellfish." MolluscanShellfish Safety.Springer Netherlands, 2014. 177-183.
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MycobacteriumAvium Paratuberculosis
CharlesG. Thornton; Kerry M. MacLellan; Judith R. Stabel; ChristineCarothers; Robert H. Whitlock; Selvin PassenApplicationof the C18-Carboxypropylbetaine Specimen Processing Method toRecovery of Mycobacterium avium subsp. paratuberculosis from RuminantTissue SpecimensJournalof Clinical Microbiology.2002;40:5;1783-1790
ZoltanS.; Gyimesi; Ilse H.; Stalis; Janice M.; Miller; Charles ODetectionof Mycobacterium avium Subspecies avium in Formalin-Fixed,Paraffin-Embedded Tissues of Captive Exotic Birds Using PolymeraseChain Reaction.Thoen Journal of Zoo and Wildlife Medicine.1999;30:3:348-353
Miller,J.M., et al,Polymerasechain reaction identification of Mycobacterium avium informalin-fixed, paraffin-embedded animal tissues.Journalof veterinary diagnostic investigation.1999.11:436-440
Paraffin-embeddedtissues
Ellingson,J. L. E., et al. "Detectionof Mycobacterium avium subspecies paratuberculosis in free-rangingbison (Bison bison) by PCR."Molecularand cellular probes19.3(2005): 219-225.
RecombinantBacterial Colonies
O.Umesh K. Reddy; Alan E. Pepper; Ibrokhim Abdurakhmonov; Sukumar Saha;Johnie N. Jenkins; Thomas Brooks, Yuksel Bolek; Kamal M. El-ZikNewDinucleotide and Trinucleotide Microsatellite Marker ResourcesTheJournal of Cotton Science.2001;5:103-113
QuiniouSM; Katagiri T; Miller NW; Wilson M; Wolters WR; WaldbieserGCConstructionand characterization of a BAC library from a gynogenetic channelcatfish Ictalurus punctatus.Genetics, selection, evolution : GSE. 2003;35(6):673-83
Bacterialartificial chromosome (BAC) vector
JM Kelley; C E Field; M B Craven; D Bocskai; U J Kim; S D Rounsley; MD Adams.HighThroughput Direct End Sequencing of BAC Clones.Nucleic Acids Research.1999.15;27(6):1539-1546
CarcinomaCell Lines & Bacterial Artificial Chromosomes (BACs)
Alsop,Amber E., Andrew E. Teschendorff, and Paul AW Edwards. "Distributionof breakpoints on chromosome 18 in breast, colorectal, and pancreaticcarcinoma cell lines." Cancergenetics and cytogenetics164.2(2006): 97-109.
PlasmidDeoxyribonucleic acid (DNA)
G.M.Huang; K. Wang; C.L. Kuo; B. Paeper; L. HoodAHigh-Throughput Plasmid DNA Preparation MethodAnalyticalBiochemtry.1994;15;223(1):35-8
RobertR. Klein, Daryl T. Morishige, Patricia E. Klein, Jianmin Dong; JohnE. Mullet.HighThroughput BAC DNA Isolation for Physical Map Construction ofSorghum.PlantMolecular Biology Reporter.1988;16(4):651-364
Deoxyribonucleicacid (DNA)
KozekovID; Turesky RJ; Alas GR; Harris CM; Harris TM; Rizzo CJ.Formationof Deoxyguanosine Cross-Links from Calf Thymus DNA Treated withAcrolein and 4-Hydroxy-2-nonenal.ChemicalResearch in Toxicology.2010;23(11):1701-1713Applied Biosystems UserBulletin. Subject: Sequencing Large DNA Templates.
DavidC. Bruce; Mark O. Mundt; Kim K. McMurry; Linda J. Meincke; Donna L.Robinson; Norman A. Doggett; Larry L. Deaven.BACLibrary End Sequencing in Support of Whole Genome AssembliesDOEJoint Genome Institute and Center for Human Genome Studies, LosAlamos National Laboratory
Dr.Domon, National Agricultural Research Center for Kyushu OkinawaRegion, Japan, Extraction of Rush DNA, unpublished personalcorrespondence, 2004.
DNAExtraction Genetic Diversity as an Indicator of Ecosystem Conditionand SustainabilityforRegional Assessments of Stream Condition in the Eastern United States
HumanFibroblasts
StephanieM Cohen, Terrence S Furey, Norman A Doggett, and David GKaufman.Genome-widesequence and functional analysis of early replicating DNA in normalhuman fibroblastsBMCGenomics.2006;7: 301
Norovirus,Adenovirus
RobertoA. Rodríguez, Lauren Thie, Christopher D. Gibbons, Mark D.Sobsey.Reducingthe effects of environmental inhibition in quantitative real-time PCRdetection of adenovirus and norovirus in recreationalseawaters.Journalof Virological Methods;2012:181(S1):43-50
Concentrationand purification of beef extract mock eluates from water samples forthe detection of enteroviruses, hepatitis A virus, and Norwalk virusby reverse transcription-PCR.Applied and environmental microbiology.1995;61(2):531-7
Blood
Krupey,J., et al,100,000+PCRs Possible from 10 ml Blood,poster Biotechniques Symposium, 2003.
HumanT-cell lymphotrophic virus I-induced adult T-cell leukemia (ATL)
JD Burton; R N Bamford; C Peters; A J Grant; G Kurys; C K Goldman; JBrennan; E Roessler; T A Waldmann.Alymphokine, provisionally designated interleukin T and produced by ahuman adult T-cell leukemia line, stimulates T-cell proliferation andthe induction of lymphokine-activated killer cellsProceedingsof the National Academy of Sciences of the United States ofAmerica.1994. 91(11): 4935-4939
Zebrafishgenomic library of bacterial artificial chromosome (BAC) clones andBAC DNA
McDermott,Brian M., et al.Transgeniclabeling of hair cells in the zebrafish acousticolateralis system.Gene Expression Patterns 10.2 (2010): 113-118.
HaiyanZheng1; Caifeng Zhao1; Swapan Roy2; Devjit Roy MD; AmenahSoherwardy2; Ravish Amin2; Matthew Kuruc21Rutgers Center forIntegrative Proteomics, Piscataway, NJ; 2Biotech Support Group LLC,Monmouth Junction, NJ.
Variantsub-populations (also known as proteoforms) of a common blood protein– Alpha-1-Antitrypsin, with functional reporting featuresseverely distinguishable between cancer patients and normal/healthyindividuals. A measurable serum cancer profile that can be modeledwith categorical biomarker proteins taken from inflammation, bloodcoagulation, tissue remodeling, and glycolysis, as well as newmarkers of unknown function.
CancerBiomarker And Wellness Protein Discovery Application Report
HaiyanZheng1; Caifeng Zhao1; Swapan Roy2; Devjit Roy MD; AmenahSoherwardy2; Ravish Amin2; Matthew Kuruc2 1Rutgers Center forIntegrative Proteomics, Piscataway, NJ; 2Biotech Support Group LLC,Monmouth Junction, NJ. Application Report -
TheComparison of the Serum Proteome in Individuals with Cancers versusthose without Cancer, and its application to Wellness
Biomarkerdiscovery from genomic analysis of singlular biomarkers or proteinpanels can be enhanced by doing albumin depletion experiments and onbead digestion. A wellness proteome strategy of over 200 spectrallyquantified proteins from healthy/normal or disease cancerous sera ofpancreatic, breast, lung patients are obtained. Individuals frompancreatic, breast and lung cancer sera are analyzed by adding abinding buffer. The bead retains serum proteome and albumin isdepleted. Subsequently the serum proteins on bead are analyzed bywash, reduction, alkylation, trypsin digestion and are labelled.Spectra from liquid chromatography mass spectrometry (LC-MS/MS) runsand proteins analyzed by Xtandem with protein filters are obtained.An example of a protein is the SERPIN family protease inhibitor. Itallows validating quantitative proteomics data, discovering newlyannotated serum proteome.
Zhenget al. AlbuVoid™Coupled to On-Bead Digestion - Tackling the Challenges of SerumProteomics.J Proteomics Bioinform 2015, 8:9
Forcancer research, serum and plasma are especially attractive sampletypes as collection of blood is common, simple and only minimallyinvasive. Yet serum samples can offer unique challenges in LC-MSproteomic analyses. The two biggest challenges being: 1) the highabundance of Albumin accounting for about 50% of the total proteinmass and, 2) proteolytic resistance, in large part due to substantialamount of glycoprotein, a modification that manifests proteolyticresistance. In this short report, we describe new methods using asurface/bead based product, AlbuVoid™, which depletes Albuminthrough a negative selection or voidance strategy, retaining the vastamount of the remaining serum proteome on the bead. We then combinethis novel enrichment, with a direct and seamless integration withTrypsin digestion, a method conventionally referred to as on-beaddigestion. We evaluated the digestion time as a parameter to identifywhether different sub-populations of peptides andproteins can be observed by LC-MS analyses. Using 2 differentallotted digestion times - 4 hours, and overnight, each with asingular 3 hour gradient LC-MS run, between 400-500 total proteinswere observed for both human and rat sera, with overlapping anddistinct sub-populations observable at each digest time. Theseresults support that the described methods gain efficiencies overother high abundance depletion and in-solution digestion workflows.We solicit that such workflows will minimize many of theinconsistencies of proteolytic hydrolysis for both discovery andquantitative serum proteomic applications.
ForHemoVoid™ LC-MS On-Bead
HemoVoid™LC-MS On-Bead Digest Application Report
Thedata shows a comparison of Trypsin digestion of the HemoVoid™bead-boundsub-proteome compared a more conventional solution digest.
ForHemogloBind™
CWan, B Borgeson, S Phanse, F Tu, K Drew, G Clark, et al. Panoramaof ancient metazoan macromolecular complexes.Nature Volume:525, Pages:339–344 Date published:(17 September2015). doi:10.1038/nature14877
Twoof BSG products, NRicher™ 6 and HemogloBind™, were ableto contribute to this rigorous examination of protein complexes. Whenour products were used as a pretreatment step in the overallworkflow, about twice the number of observations and annotationsbecame possible. This further validates that the sub-proteome biascharacteristics of NRicher™ 6 can simplify complex proteomesinto less complex sub-proteomes with efficiencies suitable for deepfunctional proteome characterization. Furthermore, this studydemonstrated the importance of a key feature implicit to all of ourproducts; that is the maintenance of functional and structuralintegrity after separations. Without that particular feature, theseadditional observations would not have been possible.
ManeeshBhargava, Kevin J. Viken, Sanjoy Dey, Michael S. Steinbach, BaolinWu, Pratik D. Jagtap, LeeAnn Higgins, Angela Panoskaltsis-Mortari,Daniel J. Weisdorf, Vipin Kumar, Mukta Arora, Peter B. Bitterman,David H. Ingbar, Chris H. Wendt. ProteomeProfiling in Lung Injury after Hematopoietic Stem CellTransplantation.Biology of Blood and Marrow Transplantation 22 (2016) 1383-1390.
Theauthors consider pulmonary complications due to infection andidiopathic pneumonia syndrome (IPS) in hematopoietic stem celltransplant (HSCT) recipients. For this, a proteomic characterizationof global bronchoalveolar lavage fluid (BALF) was studied to identifyproteins and pathways that differentiate IPS from infectious lunginjury after HSCT. Because the BALF samples appeared tinged withblood, the authors tested HemogloBind™ to determine whether theremoval of hemoglobin (in addition to high-abundance proteins) wouldimprove the depth of coverage. The article states “Hemoglobinremoval improved protein identification to 845 proteins at 1% globalFDR compared with 496 proteins with high-abundance protein depletionalone”. The authors conclude that the protein expressiondifferences provide insights into mechanisms activated in lung injuryand suggest potential therapeutic targets to augment lung repair.
Alvarez-Llamas,G., de la Cuesta, F., Barderas, M. G., Darde, V. M., Zubiri, I.,Caramelo, C., Vivanco, F. Anovel methodology for the analysis of membrane and cytosolicsub-proteomes of erythrocytes by 2-DE.Electrophoresis.2009;30:4095-4108
Authorsin this study focused on the analysis of human cytosolic and membranesub-proteomes. Hemoglobin from samples of red blood cells was studiedusing different strategies for isolation of the membrane andcytosolic fractions to determine the influence it has on proteomeprofiling by 2-DE and hemoglobin removal. Biotech Support Group'shemoglobin depletion reagent, HemogloBind™ was successfullyused to erythrocyte cells. The results showed that Hemoglobind™does have a high degree of specificity for hemoglobin and minimalinterference. Particularly interesting is how authors developed anovel combined strategy based on hypotonic lysis isolation foridentification of high molecular weight proteins (i.e. spectrin,ankyrin) by nano-LC coupled to an LTQ-Orbitrap mass spectrometer.
ForCleanascite™
Waldron,Richard T., et al. "EthanolInduced Disordering of Pancreatic Acinar Cell Endoplasmic Reticulum:an ER Stress/Defective Unfolded Protein Response Model."Cellular and Molecular Gastroenterology and Hepatology(2018).
Theauthors’ investigated the association of heavy alcoholconsumption with pancreas damage, with consideration of lightdrinking that shows the opposite effects, reinforcing proteostasisthrough the unfolded protein response orchestrated by X-box bindingprotein 1. The article states that the endoplasmic reticulum “RERfractions (100 mg) were extracted by shaking for 15 minutes at 37°Cin buffer comprising 100 mmol/L Tris pH 7.0, containing 25%acetonitrile, 0.1% sodium dodecyl sulfate, and 0.05% sodiumdeoxycholate (Buffer B), in 1.5 mL Eppendorf Protein LoBind tubesflushed of air using N2 gas. Next, 75 mL of a 1:1 slurry ofCleanascite delipidizing beads were added, and the tubes were flushedwith N2 gas and rotated end-over-end for 15 minutes at roomtemperature. Slurries were spun at 10,000 rpm for 5 minutes at roomtemperature, clarified extracts were transferred to clean tubes, andpelleted beads were discarded.” The isolated ER proteome wasthen analyzed by OXICAT LC-MS. The study concludes thatethanol-induced changes in endoplasmic reticulum protein redox andstructure/function emerge from an unfolded protein response–deficientgenetic model.
Taylor,Steven W., Nigel J. Clarke, Zhaohui Chen, and Michael J. McPhaul. "Ahigh-throughput mass spectrometry assay to simultaneously measureintact insulin and C-peptide."Clinica Chimica Acta 455 (2016): 202-208.
Inbrief, the article’s authors aimed at simultaneously measuringintact insulin and proinsulin derived C-peptide, to help predictdevelopment of diabetes mellitus, as well as in differentialdiagnosis in cases of hypoglycemia. The article states “…15µl of internal standard were added to each well followed by 50µl of Cleanascite™ delipidation reagent previously mixedinto a uniform suspension by a brief aspiration/dispense cycle withinits reagent reservoir.” The article further notes a keycomponent of the methodology as “…the use of adelipidation reagent to enhance immunocapture…The result wasgreatly enhanced recoveries and tighter CVs for the IS {internalstandard} throughout theplate”.
Megger,Dominik A., et al. "OneSample, One Shot-Evaluation of sample preparation protocols for themass spectrometric proteome analysis of human bile fluid withoutextensive fractionation."Journal of Proteomics (2016).
Inbrief, the article’s authors report methods to overcome thebiological variability of analyzing a high number of bile samples.They advance that easy sample preparation protocols are demandedrepresenting a compromise between proteome coverage and simplicity inthis study. For this, they evaluated the performance of simpleworkflows allowing for "one sample, one shot" experimentsto identify biomarker candidates for various diseases of thehepatobiliary system. In detail, sixteen different protocols withmodifications at the stages of desalting, delipidation,deglycosylation and tryptic digestion were examined. The articlestates “For delipidation, the Cleanascite™ Lipid RemovalReagent and Clarification Kit (BSG, NJ 08852, USA) was used followingmanufacturer's instructions.”. The authors concluded thatdelipidation yielded a considerable number of complementary proteinidentifications and that Cleanascite™ treatment wasindispensable for in-solution digestion methods.
Laohaviroj,Marut, et al. "A comparative proteomic analysis of bile forbiomarkers of cholangiocarcinoma." TumorBiology39.6 (2017): 1010428317705764.http://journals.sagepub.com/doi/abs/10.1177/1010428317705764
Thearticle’s authors used a quantitative proteomics approach toidentify potential tumor-associated proteins in the bile fluid of sixcholangiocarcinoma patients. Cholangiocarcinoma is a primarymalignant tumor of the bile duct epithelium and is usually detectedat an advanced stage when successful treatment is no longer possible.As the tumor originates from the bile duct epithelium, bile is anideal source of tumor biomarkers for cholangiocarcinoma. In thisstudy, Isobaric labeling, coupled with Tandem mass spectrometry, wasused to quantify protein levels in the bile of cholangiocarcinoma andcontrol patients. The article states “Cleanascite™(Biotech Support Group, USA), lipid removal reagent, was added to thebile and the sample was vertically shaken for 1 h at 4°C beforecentrifugation at 10,000 × gfor1 min.”. The authors concluded that in all, 63 proteins weresignificantly increased in cholangiocarcinoma bile compared to normalbile. Alpha-1-antitrypsin was one of the overexpressed proteins thatincreased in cholangiocarcinoma bile samples. Fecal enzyme-linkedimmunosorbent assay showed that alpha-1-antitrypsin level was able todistinguish cholangiocarcinoma patients from normal individuals,thereby making Alpha-1-antitrypsin a potential marker for earlydiagnosis of cholangiocarcinoma.
FarinaA, Dumonceau JM, Frossard JL. ProteomicAnalysis of Human Bile from Malignant Biliary Stenosis Induced byPancreatic Cancer Journalof Proteome Research.2009; 8(1):159-69
UsingCleanascite™ scientists isolated and identified hydrophobicpolypeptides in human bile and subsequently performed specializedreversed-phase chromatography and gel-filtration, and MALDI-TOF massspectrometry, to identify a small subset of five proteins. Bile fluidwas obtained by endoscopic retrograde cholangiopancreatography (ERCP)from a patient with cholangiocarcinoma. Unfractionated bile fluid wascentrifuged and partially cleared supernatant was then mixed with 250μl of Cleanascite™ followed by rotation, centrifuged, clearaway the formed lipid-micelles.
KristiansenTZ, Bunkenborg J, Gronborg M et al AProteomic Analysis of Human Bile Molecularand Cellular Proteomics.2004;3:715-728
Researchersdiscovered large amounts of lipids, bile salts in bile fluid obtainedby ERCP from patients with cholangiocarcinoma. To identify proteinsin bile fractions researchers used Cleanascite™ to precipitatelipids from “unfractionated bile” followed byone-dimensional gel electrophoresis, lectin affinity chromatographyand liquid chromatography tandem mass spectrometry.
ForNRicher™ 6
CWan, B Borgeson, S Phanse, F Tu, K Drew, G Clark, et al. Panoramaof ancient metazoan macromolecular complexes.Nature Volume:525, Pages:339–344 Date published:(17 September2015).doi:10.1038/nature14877
Twoof BSG products, NRicher™ 6 and HemogloBind™, were ableto contribute to this rigorous examination of protein complexes. Whenour products were used as a pretreatment step in the overallworkflow, about twice the number of observations and annotationsbecame possible. This further validates that the sub-proteome biascharacteristics of NRicher™ 6 can simplify complex proteomesinto less complex sub-proteomes with efficiencies suitable for deepfunctional proteome characterization. Furthermore, this studydemonstrated the importance of a key feature implicit to all of ourproducts; that is the maintenance of functional and structuralintegrity after separations. Without that particular feature, theseadditional observations would not have been possible. (Note– NRicher™ was referred to by a earlier name, Prospector™in the article)
ForNRicher™ Mx
Posterreprint from US HUPO 2014 Conference, April 6-8, 2014. Improvedproteomic and enrichment strategies
Threecommon goals prior to LC-MS are:
1.To enrich specialized sub-proteomics or alternatively - deplete highabundance protein(s)
2.Get efficient and consistent trypsin digestion
3.Streamline workflows to minimize handling, potential for variance anddecreased costs.
Toassist in all 3 aspects at once, we have developed a platform surfacechemistry with derivative products to support different applications.The surface chemistry platform called NuGel™,is based on porous silica, a chromatography industry standard and anadvantageous matrix suitable for proteome separations. With NuGel™,non-specific sites have been virtually eliminated making it an idealsupport for proteome enrichment. While many methods claim to reducethe dynamic range of protein concentrations within complex mixtures,NRicher™Mx isthe only one that is universal as it can be applied to any tissuefrom any biological source, and is suitable for small sample andprotein loads; only about 1 mg total protein being required.
BSG Highlights from US HUPO 2024 and MSACL 2024
"NRicher™ : Family Specific Enrichment For Targeted Proteomics"Abstract The need for new biomarkers to support personalized healthcare, has fostered numerous proteomic innovations. Still, a number of challenges remain. One is the preponderance of high abundance proteins and, concurrently in targeted proteomic workflows, efficiency and consistency in quantifying target peptides from different sample cohorts. This is in part due to the changing landscape of proteins/peptides not associated with the selected targets. A solution for both these challenges is now available through a suite of products called NRicher™. This bead-based technology is derived from experience of over 10 years at the forefront of manufacturing beads (i.e., ionic, hydrophobic, hydrogen bonding, aromatic, polymeric) with differential proteome binding properties. NRicher™ consists of consumable chemically derived porous beads, and an adaptability to bead cocktails, even with seemingly incompatible surface features; an important distinction of porous, over non-porous magnetic beads. NRicher™ products do not require any specialized instruments, can be processed using a standard microfuge, with adaptability to automated liquid handlers. Highlights • After NRicher , target peptides have enriched spectral signal, even as gradient times are reduced • NRicher sub-proteome enrichment can minimize acquisition time, collectively improving overall throughput, cost, and productivity • Investigate out of the Venn Diagram box. Specific target peptides that report functional and variant regions promise actionable insights and potential multiplex biomarkers for disease.
XingWang, Michael Davies, Swapan Roy and Matthew Kuruc.BeadBased Proteome Enrichment Enhances Features of the Protein ElutionPlate (PEP) for Functional Proteomic Profiling.Proteomes2015, 3(4),454-466 doi: 10.3390/proteomes3040454
Saliva
Gutiérrez,Ana, et al. "Influenceof different sample preparation strategies on the proteomicidentification of stress biomarkers in porcine saliva."BMC Veterinary Research 13.1 (2017): 375.
Gutiérrez,A. M., et al. "Detectionand first characterization of an uncommon haptoglobin in porcinesaliva of pigs with rectal prolapse by using boronic acid sampleenrichment."animal 11.5 (2017): 845-853.
Highlights from Biotech Support Group Exhibiting at World Human Proteome Congress
Biotech Support Group (BSG) exhibited at the 2025 World Human Proteome (HUPO) Congress, November 9-13, 2025 in Toronto Canada.NRicher™ Beads – for targeted serum proteomic biomarker enrichment, simplified and diversified workflows.The NRicher™ Knowledgebase, for bead enrichment of user-selected targets
New Jersey Commission On Science, Innovation And Technology Phase II Catalyst Research Grant Awarded to Biotech Support Group
Biotech Support Group announces receipt of a NEW JERSEY COMMISSION ON SCIENCE, INNOVATION AND TECHNOLOGY Phase II Catalyst Research Award of $40,000. The Research Award entitled “Evaluating the NRicher™ Bead Platform for Targeted LC-MS Quantification” will be conducted at The Rutgers Biological Mass Spectrometry Facility. This award follows from the Catalyst Phase I Award demonstrating simple workflows that generate low abundance enrichment of sub-proteomes suitable for quantitative plasma proteomics.
Toxoplasma gondii
Swapna LS, Stevens GC, Sardinha-Silva A, Hu LZ, Brand V, Fusca DD, et al."ToxoNet: A high confidence map of protein-protein interactions in Toxoplasma gondii." PLoS Comput Biol 20(6): e1012208. https://doi.org/10.1371/ journal.pcbi.1012208
Highlights from Biotech Support Group Exhibiting at World Human Proteome Congress
Biotech Support Group (BSG) exhibited at the 2025 World Human Proteome (HUPO) Congress, November 9-13, 2025 in Toronto Canada.NRicher™ Beads – for targeted serum proteomic biomarker enrichment, simplified and diversified workflows.The NRicher™ Knowledgebase, for bead enrichment of user-selected targets
New Jersey Commission On Science, Innovation And Technology Phase II Catalyst Research Grant Awarded to Biotech Support Group
Biotech Support Group announces receipt of a NEW JERSEY COMMISSION ON SCIENCE, INNOVATION AND TECHNOLOGY Phase II Catalyst Research Award of $40,000. The Research Award entitled “Evaluating the NRicher™ Bead Platform for Targeted LC-MS Quantification” will be conducted at The Rutgers Biological Mass Spectrometry Facility. This award follows from the Catalyst Phase I Award demonstrating simple workflows that generate low abundance enrichment of sub-proteomes suitable for quantitative plasma proteomics.
Toxoplasma gondii
Swapna LS, Stevens GC, Sardinha-Silva A, Hu LZ, Brand V, Fusca DD, et al."ToxoNet: A high confidence map of protein-protein interactions in Toxoplasma gondii." PLoS Comput Biol 20(6): e1012208. https://doi.org/10.1371/ journal.pcbi.1012208
Highlights from Biotech Support Group Exhibiting at World Human Proteome Congress
Biotech Support Group (BSG) exhibited at the 2025 World Human Proteome (HUPO) Congress, November 9-13, 2025 in Toronto Canada.NRicher™ Beads – for targeted serum proteomic biomarker enrichment, simplified and diversified workflows.The NRicher™ Knowledgebase, for bead enrichment of user-selected targets
New Jersey Commission On Science, Innovation And Technology Phase II Catalyst Research Grant Awarded to Biotech Support Group
Biotech Support Group announces receipt of a NEW JERSEY COMMISSION ON SCIENCE, INNOVATION AND TECHNOLOGY Phase II Catalyst Research Award of $40,000. The Research Award entitled “Evaluating the NRicher™ Bead Platform for Targeted LC-MS Quantification” will be conducted at The Rutgers Biological Mass Spectrometry Facility. This award follows from the Catalyst Phase I Award demonstrating simple workflows that generate low abundance enrichment of sub-proteomes suitable for quantitative plasma proteomics.
Toxoplasma gondii
Swapna LS, Stevens GC, Sardinha-Silva A, Hu LZ, Brand V, Fusca DD, et al."ToxoNet: A high confidence map of protein-protein interactions in Toxoplasma gondii." PLoS Comput Biol 20(6): e1012208. https://doi.org/10.1371/ journal.pcbi.1012208
Highlights from Biotech Support Group Exhibiting at World Human Proteome Congress
Biotech Support Group (BSG) exhibited at the 2025 World Human Proteome (HUPO) Congress, November 9-13, 2025 in Toronto Canada.NRicher™ Beads – for targeted serum proteomic biomarker enrichment, simplified and diversified workflows.The NRicher™ Knowledgebase, for bead enrichment of user-selected targets
New Jersey Commission On Science, Innovation And Technology Phase II Catalyst Research Grant Awarded to Biotech Support Group
Biotech Support Group announces receipt of a NEW JERSEY COMMISSION ON SCIENCE, INNOVATION AND TECHNOLOGY Phase II Catalyst Research Award of $40,000. The Research Award entitled “Evaluating the NRicher™ Bead Platform for Targeted LC-MS Quantification” will be conducted at The Rutgers Biological Mass Spectrometry Facility. This award follows from the Catalyst Phase I Award demonstrating simple workflows that generate low abundance enrichment of sub-proteomes suitable for quantitative plasma proteomics.
Toxoplasma gondii
Swapna LS, Stevens GC, Sardinha-Silva A, Hu LZ, Brand V, Fusca DD, et al."ToxoNet: A high confidence map of protein-protein interactions in Toxoplasma gondii." PLoS Comput Biol 20(6): e1012208. https://doi.org/10.1371/ journal.pcbi.1012208
MetagenomicAnalysis of the Human Mouth Virus Population and Characterisation ofTwo Lytic VirusesAhmedN. Al-Jarbou, MSc University of Leicester
AgaroseGels
Extractionand identification of electroimmunoprecipitated proteins from agarosegels.Journal of Immunological Methods Volume 330, Issues 1-2, 31 January2008, Pages 24-33
Heart
Reyes,Levy A.Newmechanisms in nitric oxide synthase related endothelial dysfunctionin the isolated heart.Diss. The Ohio State University, 2012.
SuggestedReferences
Biotech Support Group to exhibit at the 71st ASMS Conference on Mass Spectrometry and Allied Topics, June 4 - 8, 2023 in Houston, TexasFeatured at our Booth 208, will be consumable sample prep products for proteome enrichment, and on-bead digestion for rapid and streamlined workflows to improve low abundance coverage and quantification of select sub-proteomics.
TheFunctional & Chemical Proteomics Handbook03/2016
Oka,Amita R., Matthew P. Kuruc, Ketan M. Gujarathi, and Swapan Roy." FunctionalProteomic Profiling of Phosphodiesterases Using SeraFILE SeparationsPlatform." InternationalJournal of Proteomics2012(2012).
NewChemical Proteomic Methods To Access Drug-Protein Interactions
USHUPO 2014. Frontiers in Proteomics: Advancing Biology throughTechnology and Computation.
NuGel™ PROfessor™ abstractentitled "Compound-CentricDisplacement Proteomics - An Advantaged Method To Survey SmallMolecule-Protein Interactions" posterboard 096 presented at US HUPO 2014
NuGel™Related References
Patents
Monoclonalantibodies directed to the cytotoxic lymphocyte maturationfactorEuropeanPatent EP0790255
Purificationof immunoglobulins using affinity chromatography and peptideUS2006/0153834 A1
Affinity
Chaumet,Alexandre, Sandrine Castella, Laïla Gasmi, AurélieFradin, Gilles Clodic, Gérard Bolbach, Robert Poulhe, PhilippeDenoulet, and Jean-Christophe Larcher. "Proteomicanalysis of Interleukin enhancer binding factor 3 (Ilf3) and NuclearFactor 90 (NF90) interactome."Biochimie(2013).
DermotWalls, Robert McGrath and Sinéad T.LoughranADigest of Protein Purification. MethodsMolecular Biology.Volume681: 3-23 (2011)
Ehrlich,G. K., Michel, H., Chokshi, H. P. and Malick, A. W.Affinitypurification and characterization of an anti-PEG IgM. Journalof Molecular Recognition,22: 99–103 (2009).
Developmentof hepatitis B virus capsids into a whole-chain protein antigendisplay platform: New particulate Lyme disease vaccines.InternationalJournal of Medical MicrobiologyVolume298, Issues 1-2, 3 January 2008, Pages 135-142
Asensitive and high-throughput assay to detect low-abundance proteinsin serumHongtaoZhang, Xin Cheng, Mark Richter & Mark I Greene. NatureMedicine12,473 - 477 (2006)
Transformationof a L-peptide epitope into a D-peptide analog. PeptidesFrontiers of Peptide Science American Peptide Symposia,2002, Volume 5, Session XI, 769-770
Expressionand folding of an antibody fragment selected in vivo for highexpression levels in Escherichia coli cytoplasm. Researchin MicrobiologyVolume153, Issue 7, September 2002, Pages 469-474
Identificationof model peptides as affinity ligands for the purification ofhumanized monoclonal antibodies by means of phage displayJournalof Biochemical and Biophysical MethodsVolume49, Issues 1-3.2001
GeorgeK. Ehrlich, Pascal Bailon, Wolfgang Berthold.PhageDisplay Technology - Identification of Peptides as Model Ligands forAffinity Chromatography AffinityChromatography Methods in Molecular Biology,2000, Volume 147, 209-220
ADigest of Protein Purification and partial amino acid sequence of a28 kDa cyclophilin-like component of the rat liver sigmareceptor. LifeSciences,Volume 55, Issue 8, 1994.
Nachman,M., Azad, A. R. M. and Bailon, P. (1992),Efficientrecovery of recombinant proteins using membrane-based immunoaffinitychromatography (MIC). Biotechnologyand Bioengineering,40: 564–571.
Kineticaspects of membrane-based immunoaffinity chromatography.Journalof Chromatography A Volume 597, Issues 1-2, 24 April 1992, Pages167-172
Identificationof model peptides as affinity ligands for the purification ofhumanized monoclonal antibodies by means of phage display.Methods in Molecular Biology, 2000, Volume 147, 209-220
Membrane-basedreceptor affinity chromatography.Journal of Chromatography A Volume 597, Issues 1-2, 24 April 1992,Pages 155-166 9th International Symposium on Affinity Chromatographyand Biological Recognition
IonExchange
LevinW ProteinPurificationof recombinant human secretory phospholipase A2 (group II) producedin long-term immobilized cell culture.ExprPurif1992Feb;3(1):27-35.
Review
ProCipitate™,Superior Substitute to Phenol/Chloroform for DNA Isolation &Protein Extraction.by Matt Kuruc February 23, 2015
Patents
U.S.Patent Numbers 5,294,681, 5,453,493 and other patents pending.
U.S.Patent Number 5,538,870, Methodfor Preparing Nucleic Acids For Analysis And Kits Useful Therefore.
EntericViruses
Richards,Gary P., and Gail E. Greening. " Detectionof enteric viruses in shellfish." MolluscanShellfish Safety.Springer Netherlands, 2014. 177-183.
SchwabKJ; De Leon R; Sobsey MD. Immunoaffinityconcentration and purification of waterborne enteric viruses fordetection by reverse transcriptase PCR.Applied and Environmental Microbiology.1996;62(6):2086-94
SchwabKJ, De Leon R, Sobsey MD Concentrationand purification of beef extract mock eluates from water samples forthe detection of enteroviruses, hepatitis A virus, and Norwalk virusby reverse transcription-PCR.Applied and Environmental Microbiology.1995; 61(2): 531-537
LAJaykus, R De Leon and MD Sobsey. Avirion concentration method for detection of human enteric viruses inoysters by PCR and oligoprobe hybridizationAppliedand Environmental Microbiology.1996; 62(6): 2074–2080
MycobacteriumAvium Paratuberculosis
CharlesG. Thornton; Kerry M. MacLellan; Judith R. Stabel; ChristineCarothers; Robert H. Whitlock; Selvin Passen Applicationof the C18-Carboxypropylbetaine Specimen Processing Method toRecovery of Mycobacterium avium subsp. paratuberculosis from RuminantTissue Specimens Journalof Clinical Microbiology.2002;40:5;1783-1790
ZoltanS.; Gyimesi; Ilse H.; Stalis; Janice M.; Miller; Charles O Detectionof Mycobacterium avium Subspecies avium in Formalin-Fixed,Paraffin-Embedded Tissues of Captive Exotic Birds Using PolymeraseChain Reaction.Thoen Journal of Zoo and Wildlife Medicine.1999;30:3:348-353
Miller,J.M., et al, Polymerasechain reaction identification of Mycobacterium avium informalin-fixed, paraffin-embedded animal tissues.Journalof veterinary diagnostic investigation.1999.11:436-440
Paraffin-embeddedtissues
Ellingson,J. L. E., et al. "Detectionof Mycobacterium avium subspecies paratuberculosis in free-rangingbison (Bison bison) by PCR." Molecularand cellular probes 19.3(2005): 219-225.
RecombinantBacterial Colonies
O.Umesh K. Reddy; Alan E. Pepper; Ibrokhim Abdurakhmonov; Sukumar Saha;Johnie N. Jenkins; Thomas Brooks, Yuksel Bolek; Kamal M. El-Zik NewDinucleotide and Trinucleotide Microsatellite Marker Resources TheJournal of Cotton Science.2001;5:103-113
QuiniouSM; Katagiri T; Miller NW; Wilson M; Wolters WR; WaldbieserGC Constructionand characterization of a BAC library from a gynogenetic channelcatfish Ictalurus punctatus.Genetics, selection, evolution : GSE. 2003;35(6):673-83
Bacterialartificial chromosome (BAC) vector
JM Kelley; C E Field; M B Craven; D Bocskai; U J Kim; S D Rounsley; MD Adams. HighThroughput Direct End Sequencing of BAC Clones.Nucleic Acids Research.1999.15;27(6):1539-1546
CarcinomaCell Lines & Bacterial Artificial Chromosomes (BACs)
Alsop,Amber E., Andrew E. Teschendorff, and Paul AW Edwards. "Distributionof breakpoints on chromosome 18 in breast, colorectal, and pancreaticcarcinoma cell lines." Cancergenetics and cytogenetics 164.2(2006): 97-109.
PlasmidDeoxyribonucleic acid (DNA)
G.M.Huang; K. Wang; C.L. Kuo; B. Paeper; L. Hood AHigh-Throughput Plasmid DNA Preparation MethodAnalyticalBiochemtry.1994;15;223(1):35-8
RobertR. Klein, Daryl T. Morishige, Patricia E. Klein, Jianmin Dong; JohnE. Mullet. HighThroughput BAC DNA Isolation for Physical Map Construction ofSorghum.PlantMolecular Biology Reporter.1988;16(4):651-364
Deoxyribonucleicacid (DNA)
KozekovID; Turesky RJ; Alas GR; Harris CM; Harris TM; Rizzo CJ.Formationof Deoxyguanosine Cross-Links from Calf Thymus DNA Treated withAcrolein and 4-Hydroxy-2-nonenal.ChemicalResearch in Toxicology.2010;23(11):1701-1713
AppliedBiosystems User Bulletin. Subject: Sequencing Large DNA Templates.
DavidC. Bruce; Mark O. Mundt; Kim K. McMurry; Linda J. Meincke; Donna L.Robinson; Norman A. Doggett; Larry L. Deaven.BACLibrary End Sequencing in Support of Whole Genome AssembliesDOEJoint Genome Institute and Center for Human Genome Studies, LosAlamos National Laboratory
Dr.Domon, National Agricultural Research Center for Kyushu OkinawaRegion, Japan, Extraction of Rush DNA, unpublished personalcorrespondence, 2004.
DNAExtraction Genetic Diversity as an Indicator of Ecosystem Conditionand Sustainability forRegional Assessments of Stream Condition in the Eastern United States
HumanFibroblasts
StephanieM Cohen, Terrence S Furey, Norman A Doggett, and David GKaufman. Genome-widesequence and functional analysis of early replicating DNA in normalhuman fibroblastsBMCGenomics.2006;7: 301
Norovirus,Adenovirus
RobertoA. Rodríguez, Lauren Thie, Christopher D. Gibbons, Mark D.Sobsey. Reducingthe effects of environmental inhibition in quantitative real-time PCRdetection of adenovirus and norovirus in recreationalseawaters. Journalof Virological Methods;2012:181(S1):43-50
Concentrationand purification of beef extract mock eluates from water samples forthe detection of enteroviruses, hepatitis A virus, and Norwalk virusby reverse transcription-PCR.Applied and environmental microbiology.1995;61(2):531-7
Blood
Krupey,J., et al, 100,000+PCRs Possible from 10 ml Blood,poster Biotechniques Symposium, 2003.
HumanT-cell lymphotrophic virus I-induced adult T-cell leukemia (ATL)
JD Burton; R N Bamford; C Peters; A J Grant; G Kurys; C K Goldman; JBrennan; E Roessler; T A Waldmann. Alymphokine, provisionally designated interleukin T and produced by ahuman adult T-cell leukemia line, stimulates T-cell proliferation andthe induction of lymphokine-activated killer cellsProceedingsof the National Academy of Sciences of the United States ofAmerica.1994. 91(11): 4935-4939
Zebrafishgenomic library of bacterial artificial chromosome (BAC) clones andBAC DNA
McDermott,Brian M., et al. Transgeniclabeling of hair cells in the zebrafish acousticolateralis system.Gene Expression Patterns 10.2 (2010): 113-118.
HumanPeripheral Blood Mononuclear Cells (PBMCs)
Rubio-Navarro,Alfonso, et al."Hemoglobininduces monocyte recruitment and CD163-macrophage polarization inabdominal aortic aneurysm."InternationalJournal of Cardiology (2015)."
Aresearch article by authors Rubio-Navarro, Alfonso, et al in thejournal International Journal of Cardiology(http://www.internationaljournalofcardiology.com/article/S0167-5273(15)30284-9/fulltext)cites Biotech Support Group’s HemogloBind™ samplepreparation reagent to deplete hemoglobin (Hb) from conditionedmedium from healthy aortas or abdominal aortic aneurysm. The articlestates:"Conditioned mediums from AAA were incubated withHemogloBind™ reagent for hemoglobin depletion".
Inbrief, authors describe high infiltration of CD163 monocytessurrounding micro-vesicles, low expression of CD14+ & CD16-monocytes and high CD163 mRNA/protein expression is a feature ofabdominal aortic aneurysm (AAA) molecular pathology. Healthy aortaconditioned medium or complete or hemoglobin-depleted conditionedmedium from abdominal aortic aneurysm were mixed with M-CSFmacrophages to track CD163 and HLA-DR expression or hemoglobinuptake.
“Weare pleased with this data on abdominal aortic aneuryism &hemoglobin proteomics. HemogloBind™ minimizes hemoglobininterference from blood samples and allows research on cardiovascularpathology” states Swapan Roy, Ph.D., President and Founder ofBiotech Support Group.
DriedBlood Spot(DBS)/Whole Blood
Hakuna,Lovemore, et al. "Asimple assay for glutathione in whole blood."Analyst(2015).
Aresearch article in the journal Analyst(http://pubs.rsc.org/en/content/articlelanding/2015/an/c5an00345h)cites Biotech Support Group’s HemogloBind™ samplepreparation reagent to deplete hemoglobin (Hb) from whole bloodsamples containing glutathione to minimize interference from Hb inGSH fractions. Using a resorufin-acrylate fluorescent probe, GSH isquantitated in deproteinzed blood plasma and whole blood samples. Thearticle states:
RomainFragnoud, Marie Flamand, Frederic Reynier, Philippe Buchy, VasnaDuong, Alexandre Pachot, Glaucia Paranhos-Baccala and Frederic Bedin.Differential proteomic analysis of virus enriched fractions obtainedfrom plasma pools of patients with dengue fever or severe dengue. BMCInfectious Diseases (2015)15:518.http://www.ncbi.nlm.nih.gov/pubmed/26572220
Inbrief, the article’s authors report a method to compare theproteomes of virion-enriched fractions purified from plasma pools ofpatients with dengue fever or severe dengue. Virions were purified byultracentrifugation combined with Viraffinity™. Followingin-gel hydrolysis, peptides were analyzed by LC-MS. The articlestates “A technique based on ultracentifugation (Step 1)followed by concentration using…Viraffinity™, wascreated to obtain a fraction of plasma enriched with DV particles. Apositive signal corresponding to monomeric (60 kDa) and dimeric Eprotein (120 KDa) was observed in the DV-infected samples, and becamemore intense after the Viraffinity™ step…Densitometryindicated that the protein complexity of the purified samples wasreduced by roughly 350-fold compared to the unpurified samples.”.The authors conclude that the Viraffinity™ based technique ofvirion-enrichment allowed them to identify two host proteins thathave prognostic value for classifying patients with acute dengue whoare more likely to develop a severe dengue.
FeaturedApplication - microRNA from Egg Yolk
BenWade, Michelle Cummins, Anthony Keyburn and Tamsyn M.Crowley. Isolationand detection of microRNA from the egg of chickens.BMC Research Notes 2016 9:283. DOI: 10.1186/s13104-016-2084-5
Inbrief, the article’s authors report a method for thereproducible and reliable isolation of miRNA from the albumen andyolk of chicken eggs. These methods will allow the investigation ofepigenetic programming in chick development previously unknown, andhow this impacts the nutritional value of eggs for human consumption.The article states “…400 µl aliquots of theyolk/lysis solution was dispensed into five 1.5 ml microcentrifugetubes. To each of these aliquots 600 µl of Cleanascite™was added followed by rigorous vortexing until the sample becamehomogenous. The Cleanascite™ removes the lipid from this highfat tissue that would otherwise interfere with the extractionprocess…"
HemoglobinRemoval Whitepaper Describes a Range of Depletion Strategies andTheir Successful Uses
“HemoglobinRemoval - The Gold Standard Products”,April 28, 2020
ebookDescribes the Role of the Innate Immunity Proteome As a CentralModulator of Disease from Pandemic Infections toCancer
“Categorizationof Blood Based Biomarkers – Unleashing the Power of ProteomicsTo Better Understand the Innate Immune Response to Infectious andNon-infectious Inflammatory Stimuli”,April 17, 2020
StromaLiquid Biopsy™ - Blood-based biomarkers to monitor stromalconditioning in cancer. Published February, 2019.
HaiyanZheng(1),Swapan Roy(2),Amenah Soherwardy(1),Seema Rahman(2),Matthew Kuruc(2); (1)RutgersCenter for Integrative Proteomics, Piscataway, NJ; (2)BiotechSupport Group LLC, Monmouth Junction, NJ; entitled "StromaLiquid Biopsy - Proteomic Profiles for Cancer Biomarkers"
PosterReprint First Presented At AACR Annual Meeting 2016 Conference, HeldApril 17-20, 2016 New Orleans, LA USA.TheCommonality of the Cancer Serum Proteome Phenotype as analyzed byLC-MS/MS, and Its Application to Monitor Dysregulated WellnessHaiyanZheng1;Caifeng Zhao1; SwapanRoy2;Devjit Roy MD; Amenah Soherwardy2;Ravish Amin2;MatthewKuruc21RutgersCenter for Integrative Proteomics, Piscataway, NJ; 2BiotechSupport Group LLC, Monmouth Junction, NJ
TheFunctional Subproteomes of SERPIN Protease Inhibitors Are Now OpenFor LC-MS Biomarker Discovery
HaiyanZheng; Caifeng Zhao; Swapan Roy; Devjit Roy MD; Amenah Soherwardy;Ravish Amin; Matthew Kuruc. Application Report - TheComparison of the Serum Proteome in Individuals with Cancers versusthose without Cancer, and its application to Wellness
KurucMatt, Dr.Roy. Application Report -AlbuVoid™LC-MS On-Bead – Differential Expression of Cancer Sera ProteinsUsing Quantitative (iTRAQ) Proteomics
Dr.SwapanRoy; Amenah Soherwardy; Ravish Amin; Matthew Kuruc.Monmouth Junction,NJ - Application Report.AlbuSorb™Product Extension Combines Albumin and Immunoglobulin Depletion in aConsumable Format
ProCipitate™,Superior Substitute to Phenol/Chloroform for DNA Isolation &Protein Extraction.byMatt Kuruc February 23, 2015
TheFunctional & Chemical Proteomics Handbook
HemoglobinRemoval Reference Applications
Cleanascite™Lipid Removal & Clarification Reference Applications
Polack, M., et al. “Results from the UNITED study: a multicenter study validating the prognostic effect of the tumor–stroma ratio in colon cancer.” ESMO open. 9.4 (2024): 102988.
N. Diederiks, C.J. Ravensbergen, M. Treep, M. van Wezel, M. Kuruc, L. Renee Ruhaak, R.A.E. Tollenaar, C.M. Cobbaert, Y.E.M. van der Burgt, W.E. Mesker“Development of Tier 2 LC-MRM-MS protein quantification methods for liquid biopsies”Journal of Mass Spectrometry and Advances in the Clinical Lab (2022).
Ravensbergen, Cor J., et al. "“The Stroma Liquid Biopsy Panel Contains a Stromal-Epithelial Gene Signature Ratio That Is Associated with the Histologic Tumor-Stroma Ratio and Predicts Survival in Colon Cancer.” Cancers 14.1 (2022): 163.
“Stroma Liquid Biopsy™ - Blood-based biomarkers to monitor stromal conditioning in cancer” at the Companion Diagnostics Forum, October 30, 2019 in Princeton NJ.
Stroma Liquid Biopsy™ - Blood-based biomarkers to monitor stromal conditioning in cancer. Published February, 2019..
HaiyanZheng1;Swapan Roy2;Devjit Roy MD2;Amenah Soherwardy1;Seema Rathman2;Matthew Kuruc21RutgersCenter for Integrative Proteomics, Piscataway, NJ; 2Biotech SupportGroup LLC, Monmouth Junction, NJ.ApplicationReport: Stroma Liquid Biopsy – Pan-Cancer Dysregulation of theSerum Proteome.
BiotechSupport Group described a panel of protein biomarkers dysregulated incancer at the recent HUPO World Congress, that took place September17-21 in Dublin, Ireland. This research shows that there are threecommon pathways which change in the bloodstream regardless of theprimary tumor, stage, metastatic disease, or tumor burden. Fivedifferent cancers – lung, breast, pancreatic, lymph and ovary,were profiled along with normal/healthy individuals of approximateage and matched sex for comparison. The presentation is entitled“Stroma Liquid Biopsy – Biomarkers of the Dysregulationof the Serum Proteome in Cancer. It can be downloaded at: BiotechSupport Group Presents Stroma Liquid Biopsy Cancer Data at HUPO WorldCongress 2017.
PosterReprint First Presented at New Jersey Cancer Retreat, May 25, 2017.Haiyan Zheng(1), Swapan Roy(2), Amenah Soherwardy(1), SeemaRahman(2), Matthew Kuruc(2); (1)Rutgers Center for IntegrativeProteomics, Piscataway, NJ; (2)Biotech Support Group LLC, MonmouthJunction, NJ; entitled "StromaLiquid Biopsy - Proteomic Profiles for Cancer Biomarkers" .Cell. 2011;144:646-674.
ZhengH, Zhao C, Roy S, et al. TheComparison of the Serum Proteome in Individuals with Cancers versusthose without Cancer, and its application to Wellness.Poster reprint from 12th Annual US HUPO 2016 Conference, held March13 – 16, 2016 Boston, MA, USA.
ZhengH, Zhao C, Roy S, et al. TheCommonality of the Cancer Serum Proteome Phenotype as analyzed byLC-MS/MS, and Its Application to Monitor DysregulatedWellness. Posterreprint from AACR Annual Meeting 2016 Conference, held April 17-20,2016 New Orleans, LA USA
