BindPro™ For Drug Binding/Screening, Metabolomics and Protein Recovery

Product Code: BP355

Aqueous Protein Crash & Enrichment ofMetabolites/Analytes

Serum and plasma protein removal, >95%
Aqueous buffer system, simplifies analyte concentration
Aqueous Protein Crash, linearly scalable, unlike chemical precipitation or membrane filtration.
Applicable for drug binding/screening, target analytes and metabolomics
Species agnostic, tested species include: human, mouse, sheep, bovine, goat, rat, calf
BindPro™ supplied as a suspension reagent;related product - NuGel™ BindPro™ supplied as a dry powder reagent

Click Here To View BindPro™ Product Sheet

BindPro™ is a polymeric protein removal suspension reagent. It is designed as analternative to ultrafiltration for applications that require a more versatileor scaleable format. BindPro™ also can be used in lieu of solvents for drugbinding studies, especially useful for analytes that are water soluble.Consequently, BindPro™ has applications in a range of drug binding, screeningand metabolomic investigations. If desired, protein can be recovered using moderately alkaline conditions.

Product	Size	# of Samples &Sample Size*	Item No.
BindPro™	15 ml	75, 100µl Serum Samples	BP355-15
BindPro™	50 ml	250, 100µl Serum Samples	BP355-50

Equine plasma

Nishikawa, K. (2014). U.S. Patent Application No. 14/056,123.

Lipoproteins

Turner, Joseph D., R. Stuart Langley, Kelly L. Johnston, Katrin Gentil, Louise Ford, Bo Wu, Maia Graham et al. "Wolbachia lipoprotein stimulates innate and adaptive immunity through Toll-like receptors 2 and 6 to induce disease manifestations of filariasis." Journal of Biological Chemistry 284, no. 33 (2009): 22364-22378.

Patent

Bhogal, John, Shridhara Alva Karinka, Timothy P. Henning, David Cunningham, Udo Hoss, Andrew H. Naegeli, and John Latour. "Methods of Collecting and Analyzing Samples." U.S. Patent 20,120,296,189, issued November 22, 2012.

Do BSG's products work for all species?

Yes, as our products are not derived from antibodies, they are species agnostic, and have applied to humans, mice, rats, guinea pigs, goats, cows, pigs, even frogs and fish.

Can you supply bulk material only (from the kit-based products) for large volume orders?

Yes, we can adjust all of our kit products, to supply only the beads. For large orders, whether kit or bulk material, we provide special discounted quotations. Please inquire.

Can the protocol be scaled to accommodate different amounts of either volume or mass?

Yes, we provide general guidelines for use, but the protocols can usually be adjusted to accommodate either larger or small starting samples, or larger or smaller starting concentrations. We recommend first contacting us for guidance in adjusting the protocol.

Can the products be adapted to high throughput formats and automation?

Although we do not have an off-the-shelf product for 96-well formats currently, we have ongoing collaborations that can provide such formats. Please inquire.

Are the products compatible with detergents?

This has not been fully investigated, but we do not recommend use of SDS. Low concentrations, 0.1 – 0.5% of non-ionic detergents have been used with some of our products, but to what extent they alter separation performance, is unknown. If non-ionic detergents are to be used, generally we recommend that they be of no greater than 0.1% before applying to the beads. So for tissue lysates for example at higher %, dilute the sample before application.

What is the stability and shelf life of the product?

The products have a shelf life typically 6 months to 1 year. We ship at room temperature, but recommend storage at 4°C upon receipt. Do not freeze.

Do the products contain any biologically derived material?

All of our products are derived chemically, not biologically, with one exception. The "PLUS" products contain recombinant Protein A.

How can I order?

We can take on-line cart orders, which accept North American credit cards, Visa & MasterCard. It does not however, accept other international credit cards. If you would like to order direct, contact us at [email protected]. In the US, distributors Fisher Scientific and VWR can be used to order all products, even those not listed on their sites, just provide them with our catalog #. A full list of international distributors is listed on How to Order | Biotech Support Group LLC.

Performance:

Protein	BindPro™: Sample	Removal
BSA, PBS @ 30 mg/ml	1 : 1	>99%
BSA, 1%SDS @ 30 mg/ml	1 : 1	>99%
BSA, 3M GuSCN @ 30 mg/ml	1 : 1	>99%
Human Serum	2 : 1	>99%

Click Here To View BindPro™ Metabolomics Safety Data Sheet - BindPro Metabolomics

Product Code	Name	Base Price
Product Code:BP355-15	BindPro Suspension Reagent - 15 ml	Base Price:$610.00
Product Code:BP355-50	BindPro Suspension Reagent - 50 ml	Base Price:$1,065.00

BindPro™ For Drug Binding/Screening, Metabolomics and Protein Recovery

Description

References

FAQ