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NuGel™ Poly-Hydroxy

NuGel™  Poly-Hydroxy


Base Price: $550.00

Product Code: NPHX

Size (NPHX):


NuGel™ Poly-Hydroxy For Immobilization

  • Non-specific sites are virtually eliminated by a polymer coating
  • Stable across a wide pH range 2 – 10
  • 1000Å, 50µm Silica suitable for LC and batch processes
  • Applications include: Enzyme immobilization, separation and purification of biopolymers, immobilization of proteins & ligands – monoclonal antibodies, hormones, peptides, haptens, drugs, etc

Silica has been an industry standard as an advantageous matrix suitable for high performance liquid chromatography. With NuGel™, non-specific sites have been virtually eliminated making it an ideal support for affinity purification. Through a proprietary polymer coating, Silica is crosslinked forming a reactive Poly-Epoxy functionality stable across a wide pH range (pH 2 to 10). From this foundational chemistry, all of the NuGel™ affinity products are derived.

NuGel™ Poly-Hydroxy Technical Data

NuGel™ Polyhydroxy support has a terminal glycol group(cis-diol). This alpha glycol group can be activated either by sodium-m-periodate to form active aldehyde groups at the end or by a variety of leaving groups such as carbonyldiimidazole, chloroformates, sulfonates, etc. These leaving groups are easily replaced by nucleophilic ligands containing amino and thiol groups.

Technical Data
Spacer Arm Polymerized hydrophilic carbon chain
Porosity 1000Å
Average Particle Size 50um
Substitution Level 100-200 uEq/gm of Hydroxy groups

Special Features:

  • Couples ligands containing free terminal glycol groups in the presence of a Carbonyl diimidazole.
  • Couples non-polar ligands in organic solvents.
  • pH stable from 2 to 9.

Click Here To View NuGel™ Poly-Hydroxy Product Sheet

NuGel Related References

Influenza virus Wisconsin/A virus in Madin-Darby Canine Kidney (MDCK) cells

Iyer, G., Umana, J. A., & Soice, N. (2012). U.S. Patent Application No. 13/324,464.


Monoclonal antibodies directed to the cytotoxic lymphocyte maturation factor European Patent EP0790255

Purification of immunoglobulins using affinity chromatography and peptide
US 2006/0153834 A1


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Ehrlich, G. K., and P. Bailon. "Identification of model peptides as affinity ligands for the purification of humanized monoclonal antibodies by means of phage display." Journal of Biochemical and Biophysical Methods 49.1-3 (2001): 443-454.

George K. Ehrlich, Pascal Bailon, Wolfgang Berthold. Phage Display Technology - Identification of Peptides as Model Ligands for Affinity Chromatography Affinity Chromatography Methods in Molecular Biology, 2000, Volume 147, 209-220

A Digest of Protein Purification and partial amino acid sequence of a 28 kDa cyclophilin-like component of the rat liver sigma receptor
. Life Sciences , Volume 55, Issue 8, 1994.

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Journal of Chromatography A Volume 597, Issues 1-2, 24 April 1992, Pages 167-172

Identification of model peptides as affinity ligands for the purification of humanized monoclonal antibodies by means of phage display
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Membrane-based receptor affinity chromatography
. Journal of Chromatography A Volume 597, Issues 1-2, 24 April 1992, Pages 155-166 9th International Symposium on Affinity Chromatography and Biological Recognition

Ion Exchange

Levin W Protein Purification of recombinant human secretory phospholipase A2 (group II) produced in long-term immobilized cell culture. Expr Purif 1992 Feb;3(1):27-35.