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Aqueous Protein Crash &
Enrichment of Metabolites/Analytes From Serum or Plasma
- Serum and
plasma protein removal, >95%
- Ammonium
Acetate buffer system, simplifies analyte concentration
- Aqueous
Protein Crash, linearly scalable, unlike chemical precipitation or
membrane filtration.
- Fast
process, less than 30 minutes from application to separation
- Applicable
for drug binding/screening, target analytes and metabolomics
- Species agnostic, tested species include: human, mouse, sheep, bovine, goat,
rat, calf
- Dry powder
format, compatible with high throughput systems such as 96 well plate.
- NuGel™ BindPro™ supplied as a dry powder reagent; related product - BindPro™ supplied as a suspension reagent
Click Here To View NuGel™ BindPro™ Product Sheet
BindPro™ is an umbrella
trademark for polymeric reagents designed as alternatives to ultra-filtration
and solvent precipitation for applications that require protein removal and/or
concentration in a more versatile or scalable format.
NuGel™ BindPro™ is engineered onto
a bead format, based on passivated porous silica (
NuGel™ platform) covalently
bound to elastomeric poly-electrolytes.
NuGel™ BindPro™ can be used in lieu of solvents for drug binding studies, for
targeted analytes, or for metabolomic investigations.
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Product
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Size
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# of Samples &
Sample Size*
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Item No.
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NuGel™
BindPro™
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15 Preps
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15 preps, 20-30 µl of serum
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BPM55-15
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NuGel™
BindPro™
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50 Preps
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50 preps, 20-30 µl of serum
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BPM55-50
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Kit includes:
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Items Required
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15 prep
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50 Prep
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Reagent
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BindPro™ Metabolomics
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0.75 grams
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2.5 grams
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Supplied
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Binding Buffer BPMBB, PH 6.0
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8.0 ml
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25 ml
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Supplied
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Elution Buffer BPMEB
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8.0ml
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25ml
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Supplied
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SpinX Centrifuge tube filters
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15
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50
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Supplied
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Performance:
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Protein Removal Per Prep
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Removal
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BSA, 1 to 2 mg
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>95%
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Plasma or Serum 1 to 2 mg
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>95%
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References
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Lipoproteins
Turner, Joseph D., R. Stuart Langley, Kelly L. Johnston, Katrin Gentil, Louise Ford, Bo Wu, Maia Graham et al. "Wolbachia lipoprotein stimulates innate and adaptive immunity through Toll-like receptors 2 and 6 to induce disease manifestations of filariasis." Journal of Biological Chemistry 284, no. 33 (2009): 22364-22378.
Patent
Bhogal, John, Shridhara Alva Karinka, Timothy P. Henning, David Cunningham, Udo Hoss, Andrew H. Naegeli, and John Latour. "Methods of Collecting and Analyzing Samples." U.S. Patent 20,120,296,189, issued November 22, 2012.
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