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NuGel™ Poly-Aldehyde

NuGel™  Poly-Aldehyde


Base Price: $360.00

Product Code: NPAY

Size (NPAY):

Description Applications Specifications

NuGel™ Poly-Aldehyde For Immobilization

  • Couples with ligand containing amino groups.
  • Non-specific sites are virtually eliminated by a polymer coating
  • Stable across a wide pH range 2 – 10
  • 1000Å, 50µm Silica suitable for LC and batch processes
  • Application include:
    • Immobilization of proteins,enzymes, monoclonal antibodies etc.
    • Immobilization of small ligands for example: hormones, peptides, haptens etc.

Silica has been an industry standard as an advantageous matrix suitable for high performance liquid chromatography. With NuGel™, non-specific sites have been virtually eliminated making it an ideal support for affinity purification. Through a proprietary polymer coating, Silica is crosslinked forming a reactive Poly-Epoxy functionality stable across a wide pH range (pH 2 to 10). From this foundational chemistry, all of the NuGel™ affinity products are derived.

Technical Data

NuGel™ Poly-Aldehyde is a derivative of NuGel™ polyhydroxy affinity support. This affinity support contains aldehyde groups at the end of hydrophilic spacer arms and is used to couple ligands containing amino groups.

Special Features:

  • Immobilization of protein, independent of pl.
  • Immobilization of amino ligands.
  • Immobilization can be achieved at any pH between 4 and 9.
  • Protein binding capacity:
    • Murine IgG: 5-10mg per gram of support
    • Sheep serum: 5-10mg per gram of support

Click Here to View NuGel™ Poly-Aldehyde Product Sheet

NuGel Related References


Monoclonal antibodies directed to the cytotoxic lymphocyte maturation factor European Patent EP0790255

Purification of immunoglobulins using affinity chromatography and peptide US 2006/0153834 A1


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George K. Ehrlich, Pascal Bailon, Wolfgang Berthold. Phage Display Technology - Identification of Peptides as Model Ligands for Affinity Chromatography Affinity Chromatography Methods in Molecular Biology, 2000, Volume 147, 209-220

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Nachman, M., Azad, A. R. M. and Bailon, P. (1992), Efficient recovery of recombinant proteins using membrane-based immunoaffinity chromatography (MIC). Biotechnology and Bioengineering, 40: 564–571.

Kinetic aspects of membrane-based immunoaffinity chromatography. Journal of Chromatography A Volume 597, Issues 1-2, 24 April 1992, Pages 167-172

Identification of model peptides as affinity ligands for the purification of humanized monoclonal antibodies by means of phage display. Methods in Molecular Biology, 2000, Volume 147, 209-220

Membrane-based receptor affinity chromatography. Journal of Chromatography A Volume 597, Issues 1-2, 24 April 1992, Pages 155-166 9th International Symposium on Affinity Chromatography and Biological Recognition

Ion Exchange

Levin W Protein Purification of recombinant human secretory phospholipase A2 (group II) produced in long-term immobilized cell culture. Expr Purif 1992 Feb;3(1):27-35.