Home > Products > Hemoglobin Removal Kits >

HemogloBind™ Hemoglobin Depletion From Hemolyzed Serum/Plasma

HemogloBind™ Hemoglobin Depletion From Hemolyzed Serum/Plasma


Base Price: $305.00

Product Code: H0145

Size (H0145):


HemogloBind™ Hemoglobin Removal and Capture

  • Has a high degree of specificity for hemoglobin binding up to 10 mg/ml
  • Applications in blood substitutes, enzyme recovery and analytical interferences
  • Removes hemoglobin from any species including human, sheep, bovine, goat, etc
  • Removes hemoglobin from organs, tissues.
  • Hemoglobin removal from red blood cell lysate for proteomics and biomarker drug discovery
  • The flow through fractions retain their enzymatic and biological activity
  • The flow through fractions are compatible with LC-MS, activity based protein profiling and proteomic studies.

HemogloBind™ comes from a class of solid-phase, or surface-based, elastomeric poly-electrolytic surfaces that bind proteins through an empirically derived chemistry combining elements of polymer composition, cross-linking architecture and charge properties. HemogloBind™ is engineered for a high degree of selectivity and does not cross react with most common serum components, making it an excellent tool in numerous applications. These include analytical protocols where optical interference is problematic, such as bilirubin analysis and bulk serum clarification. Hemoglobin variants, as in thalassemia, bind with differential affinity towards HemogloBind™. For purification and/or analysis of hemoglobin, a modest elevation in pH will facilitate desorption of hemoglobin bound to HemogloBind™

Click Here To View HemogloBind™ Product Sheet
Click Here For The HemogloBind™ Hemoglobin Depletion Performance On Blood Sheet
Click Here For The HemogloBind™ Hemoglobin Depletion Performance On Dried Blood Spots/Cards

Hemolyzed Serum

Craig, J. R., et al. "A comparison of the anatomical and gastrointestinal functional development between gilt and sow progeny around birth and weaning." Journal of animal science (2019).


Nguyen, Anthony T., et al. "
UBE2O remodels the proteome during terminal erythroid differentiation." Science357.6350 (2017): eaan0218.

Hemocyte fractions

O’Connell, Grant C., et al. "Monocyte-lymphocyte cross-communication via soluble CD163 directly links innate immune system activation and adaptive immune system suppression following ischemic stroke." Scientific reports 7.1 (2017): 12940


Laing, Richard W., et al. "The use of an acellular oxygen carrier in a human liver model of normothermic machine perfusion." Transplantation 101.11 (2017): 2746.


Dasauni, Pushpanjali, et al. "Refractive index of blood is a potential qualitative indicator of hemoglobin disorder in humans." Journal of Proteins & Proteomics 9.3 (2018).

Cell lysates of reticulocytes

Junqueira, Caroline, et al. "
Cytotoxic CD8+ T cells recognize and kill Plasmodium vivax–infected reticulocytes." Nature medicine (2018): 1.

Whole blood aliquots

Pácal, Lukáš, et al. " Deleterious Effect of Advanced CKD on Glyoxalase System Activity not Limited to Diabetes Aetiology ." International Journal of Molecular Sciences 19.5 (2018): 1517.

Serum Samples

Dalby, M. J. (2017). The Role Of The Intestinal Microbiota In The Modulation Of Food Intake And Body Weight. OpenDalby, Matthew J.“THE ROLE OF THE INTESTINAL MICROBIOTA IN THE MODULATION OF FOOD INTAKE AND BODY WEIGHT”. Thesis Commons, 20.

Porcine D-dimer

Huang, S. (2017). The Effect of 120-kHz Ultrasound on Thrombolytic Efficacy in Porcine Thromboembolism Models (Doctoral dissertation, University of Cincinnati).

RBC perfused livers (
Hepatic primary cell line cultures)

Laing, R. W., Bhogal, R. H., Wallace, L., Boteon, Y., Neil, D. A., Smith, A., ... & Afford, S. C. (2017). The use of an acellular oxygen carrier in a human liver model of normothermic machine perfusion. Transplantation, 101(11), 2746-2756.

Total hemocyte lysates

O'Connell, Grant C., et al. "
Monocyte-Lymphocyte Cross-Communication Via Soluble CD163 Directly Links Innate Immune System Activation And Adaptive Immune System Suppression Following Ischemic Stroke." bioRxiv (2017): 144063.

Bronchoalveolar lavage fluid (BALF)

Maneesh Bhargava, Kevin J. Viken, Sanjoy Dey, Michael S. Steinbach, Baolin Wu, Pratik D. Jagtap, LeeAnn Higgins, Angela Panoskaltsis-Mortari, Daniel J. Weisdorf, Vipin Kumar, Mukta Arora, Peter B. Bitterman, David H. Ingbar, Chris H. Wendt.
Proteome Profiling in Lung Injury after Hematopoietic Stem Cell Transplantation. Biology of Blood and Marrow Transplantation 22 (2016) 1383-1390.

Cell Lines

Phanse, Sadhna, Cuihong Wan, Blake Borgeson, Fan Tu, Kevin Drew, Greg Clark, Xuejian Xiong et al. "Proteome-wide dataset supporting the study of ancient metazoan macromolecular complexes." Data in brief 6 (2016): 715-721.

Dried Blood Spot(DBS)/Whole Blood

Hakuna, Lovemore, et al. " A simple assay for glutathione in whole blood."Analyst (2015).

Whole Blood

Chalásová, Katarína, et al. "Transketolase Activity but not Thiamine Membrane Transport Change in Response to Hyperglycaemia and Kidney Dysfunction." Experimental and Clinical Endocrinology & Diabetes (2017).

Chalásová, Katarína. Functional analysis of genetic variants in genes associated with the development of glycotoxic damage. Diss. Masarykova univerzita, Lékařská fakulta, 2017.

Lahut, Suna, et al. "
Blood RNA biomarkers in prodromal PARK4 and REM sleep behavior disorder show role of complexin-1 loss for risk of Parkinson's disease." Disease Models & Mechanisms (2017): dmm-028035.

Brittain, Matthew K., Kevin G. McGarry, Robert A. Moyer, Michael C. Babin, David A. Jett, Gennady E. Platoff, and David T. Yeung. "
Efficacy of Recommended Prehospital Human Equivalent Doses of Atropine and Pralidoxime Against the Toxic Effects of Carbamate Poisoning in the Hartley Guinea Pig." International journal of toxicology (2016): 1091581816638086.

Snider, Thomas H., Kevin G. McGarry, Michael C. Babin, David A. Jett, Gennady E. Platoff Jr, and David T. Yeung. "
Acute toxicity of phorate oxon by oral gavage in the Sprague-Dawley rat." Fundamental Toxicological Sciences 3, no. 5 (2016): 195-204.

Bhargava, Maneesh, Kevin J. Viken, Sanjoy Dey, Michael S. Steinbach, Baolin Wu, Pratik D. Jagtap, LeeAnn Higgins et al. "Proteome Profiling in Lung Injury after Hematopoietic Stem Cell Transplantation." Biology of Blood and Marrow Transplantation (2016).

Snider, Thomas H., Christina M. Wilhelm, Michael C. Babin, Gennady E. Platoff Jr, and David T. Yeung. "
Assessing the therapeutic efficacy of oxime therapies against percutaneous organophosphorus pesticide and nerve agent challenges in the Hartley guinea pig."The Journal of Toxicological Sciences 40, no. 6 (2015): 759-775.

Blood Plasma

Johns, Michael, et al. "SR-135, a peroxynitrite decomposing catalyst, enhances β-cell function and survival in B6D2F1 mice fed a high fat diet."Archives of Biochemistry and Biophysics (2015).

Asleh, Rabea, et al. " Haptoglobin Genotype-dependent Differences in Macrophage Lysosomal Oxidative Injury." Journal of Biological Chemistry289.23 (2014): 16313-16325.

Biological Fluids

J Krupey - United States Patent: 10/180,053, 2002 Removal of extraneous substances from biological fluids containing nucleic acids and the recovery of nucleic acids

Lung Tissue Specimens & Pulmonary Research (Acute Respiratory Distress Syndrome & Acute Hypoxic Failure)
Bhargava, Maneesh""Proteomic Studies in Acute Hypoxic Respiratory Failure." PhD diss., UNIVERSITY OF MINNESOTA. (2015).

Red Blood Cell (RBC) - Lysates.

Kyoungsook Park, Christopher D. Saudek, and Gerald W. Hart Increased Expression of β-N-Acetylglucosamindase (O-GlcNAcase) in Erythrocytes from Prediabetic and Diabetic Individuals. Diabetes.2010;59(7):1845-50.

Barasa, Benjamin, and Monique Slijper. " Challenges for red blood cell biomarker discovery through proteomics." Biochimica et Biophysica Acta (BBA)-Proteins and Proteomics 1844.5 (2014): 1003-1010

Stored Blood Products

Delobel J., Rubin O., Prudent M., Crettaz D., Tissot J.-D., Lion N.(2010) Biomarker Analysis of Stored Blood Products: Emphasis on Pre-Analytical Issues. International Journal of Molecular Sciences. 11(11):4601-4617

Red Blood Cells/Forensic Research

Danielson, Phillip B. "Isolation of Highly Specific Protein Markers for the Identification of Biological Stains: Adapting Comparative Proteomics to Forensics." (2011).

Red Blood Cells (RBC) - Blood.

Carvalho, Ana Sofia, Manuel S. Rodriguez, and Rune Matthiesen. "Red Blood Cells in Clinical Proteomics." Serum/Plasma Proteomics: Methods and Protocols (2017): 173-181.

Wilhelm, Christina M., et al. A comprehensive evaluation of the efficacy of leading oxime therapies in guinea pigs exposed to organophosphorus chemical warfare agents or pesticides. Toxicology and Applied Pharmacology Available online 31 October 2014. doi:10.1016/j.taap.2014.10.009

Hikosaka, Keisuke, et al. "Deficiency of Nicotinamide Mononucleotide Adenylyltransferase 3 (Nmnat3) Causes Hemolytic Anemia by Altering the Glycolytic Flow in Mature Erythrocyte"Journal of Biological Chemistry(2014): jbc-M114.

McGarry, Kevin G., et al. " Evaluation of HemogloBind™ treatment for preparation of samples for cholinesterase analysis." (2013). Advances in Bioscience and Biotechnology, 2013, 4, 1020-1023

Alvarez-Llamas, Gloria, Fernando de la Cuesta, Maria G. Barderas, Irene Zubiri, Maria Posada-Ayala, and Fernando Vivanco. " Characterization of Membrane and Cytosolic Proteins of Erythrocytes." In Vascular Proteomics, pp. 71-80. Humana Press, 2013.

Alvarez-Llamas, G., de la Cuesta, F., Barderas, M. G., Darde, V. M., Zubiri, I., Caramelo, C., Vivanco, F. A novel methodology for the analysis of membrane and cytosolic sub-proteomes of erythrocytes by 2-DE.Electrophoresis.2009;30:4095-4108

Zihao Wang, Kyoungsook Park, Frank Comer1, Linda C. Hsieh-Wilson, Christopher D. Saudek, Gerald W. Hart. Site-Specific GlcNAcylation of Human Erythrocyte Proteins: Potential Biomarker(s) for Diabetes Mellitus. Diabetes.2008;58, 309-317.

Datta, Pradip. Effect of Hemolysis, High Bilirubin, Lipemia, Paraproteins, and System Factors on Therapeutic Drug Monitoring. Handbook of Drug Monitoring Methods.2008; 97-109.

Yuichi Miki, Tomoki Tazawa, Kazuya Hirano, Hideki Matsushima, Shoko Kumamoto, Naotaka Hamasaki, Tomohiro Yamaguchi, Masatoshi Beppu. Clearance of oxidized erythrocytes by macrophages: Involvement of caspases in the generation of clearance signal at band 3 glycoprotein. Biochemical and Biophysical Research Communications.2007; 363(1):57-62

Sarawathi,et al., Relative quantification of glycated Cu-Zn superoxide dismutase in erythrocytes by electrospray ionization mass spectrometry, Biochimica et Biophysica Acta. 1999.1426(3):483-90


Krishna, Neel K., and Kenji Cunnion. "
Derivative Peptide Compounds and Methods of Use." U.S. Patent No. 20,160,376,322. 29 Dec. 2016.

Person, N.B., Effect Of HemogloBind™ On Interference Reduction In Bilirubin Analysis.poster Clinichem, 1995.


Nishida-Aoki, Nao, et al. "Disruption of Circulating Extracellular Vesicles as a Novel Therapeutic Strategy against Cancer Metastasis." Molecular Therapy 25.1 (2017): 181-191.

Baion, C.M. & Ali, A.C.
Evaluation Of HemogloBind™ For Removal Of O-Raffinose Crosslinked Hemoglobin (Hemolink™) From Serum, poster AACC Meeting 1997.


Padilla, S., Convenient Method for Decreasing the Amount of Hemoglobin in Tissue Samples Without Affecting the Level of Cholinesterase Activity, unpublished personal correspondence, 1994.