Hemolyzed Red Blood Cells Biological Fluids
E. McKiernan, Phillip B. Danielson, Catherine O. Brown, Masha
Signaevsky, Christian G. Westring and Kevin M. Legg, Developmental
Validation of a Multiplex Proteomic Assay for the Identification of
Forensically Relevant Biological Fluids, Forensic Science
International, (2021) https://www.sciencedirect.com/science/article/pii/S0379073821002280?via%3Dihub
Peripheral blood derived mononuclear cells whole cell lysate samples
Kaneko, Tomonori, et al. " System-wide hematopoietic and immune signaling aberrations in COVID-19 revealed by deep proteome and phosphoproteome analysis. " Research Square preprint (2021).
Blood cell lysates
Yamagishi, Yoshikazu, Hirotaro Iwase, and Yasumitsu Ogra. "Post-Mortem Changes of Methomyl in Blood with Hemoglobin." Chemical Research in Toxicology.
Reticulocytes (Retics) lysates
Hojo-Souza NS, de Azevedo PO, de Castro JT, Teixeira-Carvalho A, Lieberman J, et al. (2020) Contributions of IFN-γ and granulysin to the clearance of Plasmodium yoelii blood stage. PLOS Pathogens 16(9): e1008840. https://doi.org/10.1371/journal.ppat.1008840
Rebecca Marie, Kenneth Sherlock, and Matthew Baylis.
study of British wild deer for evidence of Schmallenberg virus
Veterinary Record (2020).
Host red blood cells
Dziekan, Jerzy Michal, et al. "Cellular
thermal shift assay for the identification of drug–target
interactions in the Plasmodium falciparum proteome.
" Nature Protocols (2020): 1-41.
HemogloBind ™ Reference Applications –
Nguyen, Anthony T., et al.
UBE2O remodels the proteome during terminal erythroid
" Science 357.6350
During reticulocyte maturation, the proteome is remodeled
through the programmed elimination of most generic constituents of the cell, in
parallel with abundant synthesis of hemoglobin. The study used multiplexed
quantitative proteomics to identify candidate substrates of UBE2O, an E2
(ubiquitin-conjugating) enzyme, in an unbiased and global manner. Because
of the overly abundant presence of Hemoglobin, selective depletion of
Hemoglobin was necessary. T
he article states “Reticulocytes were lysed
by vortexing for 5 minutes at room temperature… An additional 10 bed vol of
Hemoglobind™ suspension was added to the samples, which were then vortexed for
another 10 min at room temperature followed by 4 min of centrifugation at 10000
g. The supernatants, which contain hemoglobin-depleted sample,
were … processed for TMT quantification.
Serum Exosome Analyses
Nishida-Aoki, Nao, et al.
"Disruption of Circulating Extracellular Vesicles as a Novel Therapeutic
Strategy against Cancer Metastasis."
Molecular Therapy 25.1 (2017):
This study considers that therapeutic strategies
targeting cancer-derived extracellular vesicles (EVs) hold great promise
because of the possibility they reposition microenvironments to accommodate
metastasis. The researchers report on a novel strategy of therapeutic antibody
treatment to target cancer-derived EVs and inhibit the metastasis of breast
cancer in a mouse model. The article states “Hemoglobin was accumulated with
HemogloBind™ beads (Biotech support group, Monmouth Junction NJ, USA) followed
by 0.22 μm filtration. Then, the EVs in the sera were concentrated by
Wan, B Borgeson, S Phanse, F Tu, K Drew, G Clark, et al.
of ancient metazoan macromolecular complexes.
Pages:339–344 Date published:(17 September 2015). doi:10.1038/nature14877
Lahut, Suna, et al. "Blood RNA
biomarkers in prodromal PARK4 and REM sleep behavior disorder show role of
complexin-1 loss for risk of Parkinson's disease."
Disease Models &
(2017): dmm-028035. http://dmm.biologists.org/lookup/doi/10.1242/dmm.028035
In this study, Parkinson’s disease
progression is investigated through the accumulation and aggregation of the
lipid-binding SNARE complex component alpha-synuclein (SNCA) which underlies
vulnerability and defines its stages. The authors studied blood samples from a
new large pedigree with
SNCA gene duplication (PARK4 mutation), to
identify effects of SNCA gain-of-function as potential disease biomarkers. The
article states “For protein extraction from the EDTA tubes, 300 μl blood were
lysed with equal amount of 1% SDS-RIPA buffer [50 mM Tris-HCl (pH 8.0), 150 mM
NaCl, 1 mM EDTA, 1 mM EGTA, 1% Igepal CA-630 (Sigma), 0.5% sodium deoxycholate,
0.1% SDS, 1 mM PMSF and one tablet Complete Protease Inhibitor Cocktail
(Roche)] and sonicated for 10 sec. The blood lysates were rotated at 4 °C for
30 min and centrifuged at 4 °C for 30 min. The supernatants were depleted in
hemoglobin content using a commercial kit (HemogloBind™) following the
Katarína, et al. "Transketolase Activity but not Thiamine Membrane
Transport Change in Response to Hyperglycaemia and Kidney
Experimental and Clinical Endocrinology &
Diabetic kidney disease, a common complication of both type 1 and type 2
diabetes, is associated with significant morbidity and mortality, and
represents the most common cause of chronic kidney disease. The study
hypothesized that protective pentose phosphate pathway action in diabetes might
be compromised by limited intracellular availability of an active transketolase
cofactor thiamine diphosphate (TDP). To evaluate the levels of thiamine
tranporter proteins in whole blood, t
he article states “ For protein isolation, whole blood aliquots were lysed
with water and haemoglobin was removed using HemogloBind™ (Biotech Support
Group) according to manufacturer’s instructions…”.
Agnostic – Applications to Different Species
Snider, Thomas H., Christina M. Wilhelm, Michael C. Babin,
Gennady E. Platoff Jr, and David T. Yeung. "
the therapeutic efficacy of oxime therapies against percutaneous
organophosphorus pesticide and nerve agent challenges in the Hartley guinea pig
Journal of Toxicological Sciences 40, no. 6 (2015): 759-775.
Acetylcholine is an essential neurotransmitter, and inhibitors of
cholinesterases(ChEs) are potent toxins. A primary component of
anti-organophosphorus therapy is an oxime reactivator to rescue inhibited
acetylcholinesterases. For this, clinical signs of toxicity can be measured
from blood cholinesterase [Acetylcholinesterase (AChE) and
butyrylcholinesterase (BChE)] activity utilizing a modified Ellman's method.
Biotech Support Group’s unique solid-phase polymer for hemoglobin depletion,
was used for pretreatment. The article states “Briefly, whole blood samples
were treated with HemogloBind™ which interferes with the ChE activity assay due
to spectral overlap.”
Craig, J. R., et al. "A comparison of the anatomical and gastrointestinal
functional development between gilt and sow progeny around birth and weaning
of animal science
Gilt progeny (GP) often have
restricted growth performance and health status in comparison to sow progeny
(SP) from birth. To better understand underlying mechanisms, the study aimed to
compare differences in growth and development between GP and SP in the first 24
h after birth and in the peri-weaning period.
samples were quite hemolysed after collection and processing, it became
necessary to use HemogloBind™ to allow for better detection of IgG by ELISA.
article states “As per the manufacturer’s instructions, 250 μL of Hemoglobind
was added to 250 μL of hemolyzed serum…
Parvathi S. Kumar, Haree K. Pallera, Pamela S. Hair, Magdielis
Gregory Rivera, Tushar A. Shah, Alice L. Werner,Frank A. Lattanzio, Kenji M.
Neel K. Krishna. Peptide
inhibitor of complement C1 modulates acute intravascular hemolysis of
mismatched red blood cells in rats.
TRANSFUSION Volume 00,
May 2016. doi:10.1111/trf.13674.
In brief, the study evaluated the role of a peptide inhibitor of complement C1
(PIC1) in an animal model of acute intravascular hemolysis in both prevention
and rescue scenarios. The authors state "To remove free Hb that may cause
optical interference in bilirubin analysis, we treated all the samples with Hb
depletion from hemolyzed serum/plasma (HemogloBind, Biotech Support Group).
Bilirubin concentration was then measured with a Bilirubin Assay Kit
(Sigma-Aldrich, St. Louis, MO)."
Laing, Richard W., et al.
The use of an acellular oxygen carrier in a human liver
model of normothermic machine perfusion
." Transplantation 101.11
Red cell lysates
Grant C., et al. "
cross-communication via soluble CD163 directly links innate immune
system activation and adaptive immune system suppression following
Park, Christopher D. Saudek, and Gerald W. Hart
Expression of β-N-Acetylglucosamindase (O-GlcNAcase) in Erythrocytes
from Prediabetic and Diabetic
J., Rubin O., Prudent M., Crettaz D., Tissot J.-D., Lion
Analysis of Stored Blood Products: Emphasis on Pre-Analytical
International Journal of Molecular Sciences. 11(11):4601-4617
Gloria, Fernando de la Cuesta, Maria G. Barderas, Irene Zubiri, Maria
Posada-Ayala, and Fernando Vivanco. "
of Membrane and Cytosolic Proteins of Erythrocytes.
In Vascular Proteomics, pp. 71-80. Humana Press,
Alvarez-Llamas, G., de la Cuesta, F., Barderas, M.
G., Darde, V. M., Zubiri, I., Caramelo, C., Vivanco, F.
novel methodology for the analysis of membrane and cytosolic
sub-proteomes of erythrocytes by
Wang, Kyoungsook Park, Frank Comer1, Linda C. Hsieh-Wilson,
Christopher D. Saudek, Gerald W. Hart.
GlcNAcylation of Human Erythrocyte Proteins: Potential Biomarker(s)
for Diabetes Mellitus
Miki, Tomoki Tazawa, Kazuya Hirano, Hideki Matsushima, Shoko
Kumamoto, Naotaka Hamasaki, Tomohiro Yamaguchi, Masatoshi
of oxidized erythrocytes by macrophages: Involvement of caspases in
the generation of clearance signal at band 3 glycoprotein
Biochemical and Biophysical Research Communications.2007;
quantification of glycated Cu-Zn superoxide dismutase in erythrocytes
by electrospray ionization mass spectrometry
Biochimica et Biophysica Acta. 1999.1426(3):483-90