NRicher™ Mx
General Enrichment for All Biofluids and Tissue Lysates
· Consumable chemically derived beads, species
agnostic as they are not derived from antibodies
·
Enrich low abundance proteomes from any source, from sera/plasma to
cell lysates from both animals and humans, >90% Albumin removal
·
Scalable
protocol from small to large sample volumes, from 10 to 500 µl, and low to high protein concentrations
·
Enriched
sub-proteomes, for better target signal quantitation between samples
·
Does not require any specialized instruments,
just a standard microfuge
·
Bead format suitable for automation
compatibility, please inquire
·
On-Bead digestion for LC-MS analysis, or
optional elution for any functional, enzymatic, or immunoassay analysis
NRicher™
Mx
employs the use of a bead cocktail, which allows for one, rather than multiple
LC-MS analyses to establish dynamic range compression. NRicher™ Mx is
thus an all-purpose proteomic enrichment product that can be used for any
sample type, from biofluids to tissue lysates. It is compatible with up to 1%
non-ionic detergent concentrations.
NRicher™ Mx is particularly useful for discovery and quantification of membrane proteins, targets of over 50% of all therapeutic drugs, performing a variety of functions including:
- Receptors which relay information between internal and external environments
- Adhesion
- Transport
of molecules and ions into the cell
Membrane proteins are observable in serum/plasma due to ectodomain shedding, the proteolytic cleavage
of cell surface proteins resulting in the loss of the extracellular domains.
This mechanism is important in a variety of normal and pathological processes,
including growth factor signaling, inflammation and cell
survival.
NRicher™ Mx provides excellent 2-3X enrichment of membrane proteins from serum/plasma, the vast majority of which are not observable in neat serum. Circulating soluble forms of membrane proteins (i.e. sCD163) have been described as potential biomarkers of inflammatory disease and cancer. As such, they offer a rich source of potential personalized healthcare biomarkers.
Another example of NRicher™ Mx enrichment is α-Synuclein, a biomarker for Parkinsons Disease, observed with NRicher™ Mx, but not observed in neat serum.
Click Here To View NRicher™ Mx Product Sheet
*Final Pricing shown in cart
