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NRicher™ Mx
General Enrichment for All Biofluids and Tissue Lysates
·Consumable chemically derived beads, species
agnostic as they are not derived from antibodies
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Enrich low abundance proteomes from any source, from sera/plasma to
cell lysates from both animals and humans, >90% Albumin removal
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Scalable
protocol from small to large sample volumes, from 10 to 500 µl, and low to high protein concentrations
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Enriched
sub-proteomes, for better target signal quantitation between samples
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Does not require any specialized instruments,
just a standard microfuge
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Bead format suitable for automation
compatibility, please inquire
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On-Bead digestion for LC-MS analysis, or
optional elution for any functional, enzymatic, or immunoassay analysis
NRicher™
Mx
employs the use of a bead cocktail, which allows for one, rather than multiple
LC-MS analyses to establish dynamic range compression.
NRicher™ Mx is
thus an all-purpose proteomic enrichment product that can be used for any
sample type, from biofluids to tissue lysates. It is compatible with up to 1%
non-ionic detergent concentrations.
NRicher™ Mx is particularly useful for discovery and quantification of membrane proteins, targets of over 50% of all therapeutic drugs, performing a variety of functions including:
- Receptors which relay information between internal and external environments
- Adhesion
- Transport
of molecules and ions into the cell
Click Here To View NRicher™ Mx Product Sheet
Membrane proteins are observable in serum/plasma due to ectodomain shedding, the proteolytic cleavage
of cell surface proteins resulting in the loss of the extracellular domains.
This mechanism is important in a variety of normal and pathological processes,
including growth factor signaling, inflammation and cell
survival.
NRicher™ Mx provides excellent 2-3X enrichment of membrane proteins from serum/plasma, the vast majority of which are not observable in neat serum. Circulating soluble forms of membrane proteins (i.e. sCD163) have been described as potential biomarkers of inflammatory disease and cancer. As such, they offer a rich source of potential personalized healthcare biomarkers.
Another example of NRicher™ Mx enrichment is α-Synuclein, a biomarker for Parkinsons Disease, observed with NRicher™ Mx, but not observed in neat serum.
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Product
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Size
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Total serum/plasma
samples processed
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Item No.
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NRicher™ Mx
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10 Preps
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10 x (2-4) mg total protein samples
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NIMX-10
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NRicher™ Mx
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50 Preps
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50 x (2-4) mg total protein samples
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NIMX-50
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References
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Swapna LS, Stevens GC, Sardinha-Silva A, Hu LZ, Brand V, Fusca DD, et al. (2024 )
ToxoNet: A high confidence map of protein-protein interactions in Toxoplasma gondii. PLoS Comput Biol 20(6):
e1012208. https://doi.org/10.1371/ journal.pcbi.1012208
The article states we used affinity beads (NuGel PROspector) to pre-enrich Toxoplasma gondii lysate to capture five distinct
subproteomes. [Note: NuGel PROspector beads are now part of the NRicher™ platform.]. When comparing the 5 different
subproteomes, there is clearly different selection biases amongst the 5 surface chemistries. Also, many of the proteins
observed from the NRicher™ beads, were not observed in the Ion exchange fractions demonstrating the importance of
combining different modes (ionic, hydrophobic, etc.) of separation to alter selection properties, and consequently improving
overall proteome coverage.
Wan, C., Borgeson, B., Phanse, S. et al. Panorama of ancient metazoan macromolecular complexes. Nature 525, 339–344 (2015). hXps://doi.org/10.1038/nature14877
Six different NRicher™ beads (described with an old tradename PROspector) were used as an enrichment step in the overall
workflow; about twice the number of observations and annotations became possible. This further validates that the subproteome bias characteristics of the NRicher™ surface chemistry platform can simplify complex proteomes into enriched subproteomes with efficiencies suitable for deep functional proteome characterization.
Whitepaper - NRicher™: A Low Abundance Proteome Enrichment Platform With Seamless Integration of On-Bead Digestion
The NRicher™ Advantage is described: • Consumable chemically derived NuGel™ beads, species agnostic as they are not
derived from antibodies • Does not require any specialized instruments, just a standard microfuge • Use of bead cocktails
allows for one, rather than multiple LC-MS analyses • Functionally active sub-proteomes after separations, for any
orthogonal functional, enzymatic, or immunoassay analysis
https://www.biotechsupportgroup.com/v/vspfiles/templates/257/pdf/BiotechSupportGroup-NRicher-Whitepaper.pdf
NRicher : Family Specific Enrichment For Targeted Proteomics – Poster US HUPO 2024
The need for new biomarkers to support personalized healthcare, has fostered numerous proteomic innovations. Still, a
number of challenges remain. One is the preponderance of high abundance proteins and, concurrently in targeted proteomic
workflows, efficiency and consistency in quantifying target peptides from different sample cohorts. This is in part due to the
changing landscape of proteins/peptides not associated with the selected targets. A solution for both these challenges is now
available through a suite of products called NRicher .
https://www.biotechsupportgroup.com/v/vspfiles/templates/257/pdf/NRicher%20poster%20small.pdf
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