KinaSorb Kinase (& ATP binding proteins) Enrichment Reagent

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KinaSorb™ Kinase (& ATP binding proteins) Enrichment Reagent

Product Code: KE785

KinaSorb™ Kinase (& ATP binding proteins) Enrichment Reagent

Non-covalent immobilization of phosphate group with optimal nucleotide orientation & specificity
Enrichment 3-5X Kinase enrichment, protein recoverable, ~200 µg
60 minute, scaleable protocol compatible with functional assays, electrophoresis and LC-MS
Phosphatase activity & cyclic nucleotide phosphodiesterase (PDE) activity not detectable
Improves protein normalization when comparing heterogeneous tissues
On-bead digestion protocols for LC-MS annotation

Click Here To View Kinasorb™ Product Sheet

KinaSorb™ is a new reagent kit used for the enrichment and isolation of kinases. ATP – the common substrate for kinases, is reversibly immobilized with optimal orientation and specificity, to metallic oxide particles, producing a single-use highly efficient enrichment method for kinase & other ATP binding proteins. The standard prep protocol starts with 100 µl of clarified cellular extracts, or approximately 2 mg total cellular extract protein, but the process can be scaled up or down to accommodate different sample volumes and protein concentrations. The kit includes all necessary reagents for immediate use.

Product	size	Item No.
KinaSorb™	10 Preps	KE785-10
KinaSorb™	50 Preps	KE785-50

Xing Wang, Michael Davies, Swapan Roy and Matthew Kuruc. Bead Based Proteome Enrichment Enhances Features of the Protein Elution Plate (PEP) for Functional Proteomic Profiling . Proteomes2015, 3(4), 454-466 .
doi: 10.3390/proteomes3040454

Do BSG's products work for all species?

Yes, as our products are not derived from antibodies, they are species agnostic, and have applied to humans, mice, rats, guinea pigs, goats, cows, pigs, even frogs and fish.

Can you supply bulk material only (from the kit-based products) for large volume orders?

Yes, we can adjust all of our kit products, to supply only the beads. For large orders, whether kit or bulk material, we provide special discounted quotations. Please inquire.

Can the protocol be scaled to accommodate different amounts of either volume or mass?

Yes, we provide general guidelines for use, but the protocols can usually be adjusted to accommodate either larger or small starting samples, or larger or smaller starting concentrations. We recommend first contacting us for guidance in adjusting the protocol.

Can the products be adapted to high throughput formats and automation?

Although we do not have an off-the-shelf product for 96-well formats currently, we have ongoing collaborations that can provide such formats. Please inquire.

Are the products compatible with detergents?

This has not been fully investigated, but we do not recommend use of SDS. Low concentrations, 0.1 – 0.5% of non-ionic detergents have been used with some of our products, but to what extent they alter separation performance, is unknown. If non-ionic detergents are to be used, generally we recommend that they be of no greater than 0.1% before applying to the beads. So for tissue lysates for example at higher %, dilute the sample before application.

What is the stability and shelf life of the product?

The products have a shelf life typically 6 months to 1 year. We ship at room temperature, but recommend storage at 4°C upon receipt. Do not freeze.

Do the products contain any biologically derived material?

All of our products are derived chemically, not biologically, with one exception. The "PLUS" products contain recombinant Protein A.

How can I order?

We can take on-line cart orders, which accept North American credit cards, Visa & MasterCard. It does not however, accept other international credit cards. If you would like to order direct, contact us at [email protected]. In the US, distributors Fisher Scientific and VWR can be used to order all products, even those not listed on their sites, just provide them with our catalog #. A full list of international distributors is listed on How to Order | Biotech Support Group LLC.

Kit includes:

Kit Contains:	KinaSorb™ 10	KinaSorb™ 50
Activated KinaSorb™ reagent suspension	0.4 ml	2.0 ml
Cleanascite™ reagent suspension	1 ml	5 ml
KE-BB Binding Buffer	20 ml	100 ml
ATP (dry powder) tube {Store at -20°C}	0.001 gram	0.005 gram
ATP solution buffer	0.25 ml	1.25 ml
KE-EB Elution Buffer	1 ml	5 ml

SDS:
SDS-KinaSorb™ (Catalog Order # KE785-10, KE785-50)

Product Code	Name	Base Price
Product Code:KE785-10	KinaSorb	Base Price:$495.00
Product Code:KE785-50	KinaSorb	Base Price:$1,835.00

KinaSorb™ Kinase (& ATP binding proteins) Enrichment Reagent

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