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NuGel™ Phenyl Boronic Acid

NuGel™  Phenyl Boronic Acid


Base Price: $795.00

Product Code: NPBA

Size (NPBA):


Polymer Coated Silica Affinity Matrices For Glycoprotein Enrichment

  • Non-specific sites are virtually eliminated by a polymer coating
  • Stable across a wide pH range 4 – 10
  • 1000Å, 50µm suitable for LC and batch processes
  • Cis-diol specific, enriches heterogeneous sets of glycoproteins
  • Unmasks glycoproteins from high abundance proteins, most notably albumin
  • Efficient new surface technology ideal for proteomic applications
  • Enriches glycoproteins from blood, serum, plasma, tissue or cell culture media.
  • Sorbitol elution at pH 7.5 – 8.5
  • Adaptable to liquid chromatography (LC) and high-throughput 96-well format

NuGel™ Phenyl Boronic Acid is a derivative of NuGel™ polyepoxy silica affinity support. This affinity support contains phenyl boronic groups at the end of hydrophilic spacer arms and is used to bind ligands containing cis-diols, glycoproteins.

NuGel™ Phenyl Boronic Acid Technical Data

Characteristics Of The Matrix
Spacer Arm Polymerized hydrophilic carbon chain
Porosity 1000Å
Average Particle Size 50um

Special Features:

  • Binds ligands containing 1,2 cis-diol groups of glycoproteins.
  • pH stable from 4 to 10.

Click Here To View NuGel™ Phenyl Boronic Acid Product Sheet

NuGel Related References


Monoclonal antibodies directed to the cytotoxic lymphocyte maturation factor European Patent EP0790255

Purification of immunoglobulins using affinity chromatography and peptide
US 2006/0153834 A1


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Expression and folding of an antibody fragment selected in vivo for high expression levels in Escherichia coli cytoplasm
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Identification of model peptides as affinity ligands for the purification of humanized monoclonal antibodies by means of phage display
Journal of Biochemical and Biophysical Methods Volume 49, Issues 1-3.2001

George K. Ehrlich, Pascal Bailon, Wolfgang Berthold. Phage Display Technology - Identification of Peptides as Model Ligands for Affinity Chromatography Affinity Chromatography Methods in Molecular Biology, 2000, Volume 147, 209-220

A Digest of Protein Purification and partial amino acid sequence of a 28 kDa cyclophilin-like component of the rat liver sigma receptor
. Life Sciences , Volume 55, Issue 8, 1994.

Nachman, M., Azad, A. R. M. and Bailon, P. (1992), Efficient recovery of recombinant proteins using membrane-based immunoaffinity chromatography (MIC). Biotechnology and Bioengineering, 40: 564–571.

Kinetic aspects of membrane-based immunoaffinity chromatography.
Journal of Chromatography A Volume 597, Issues 1-2, 24 April 1992, Pages 167-172

Identification of model peptides as affinity ligands for the purification of humanized monoclonal antibodies by means of phage display
. Methods in Molecular Biology, 2000, Volume 147, 209-220

Membrane-based receptor affinity chromatography
. Journal of Chromatography A Volume 597, Issues 1-2, 24 April 1992, Pages 155-166 9th International Symposium on Affinity Chromatography and Biological Recognition

Ion Exchange

Levin W Protein Purification of recombinant human secretory phospholipase A2 (group II) produced in long-term immobilized cell culture. Expr Purif 1992 Feb;3(1):27-35.