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HemogloBind™ Blood Card Kit

HemogloBindâ„¢ Blood Card Kit


 
 


Base Price: $395.00


Product Code: H0145BC


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Description
 

Hemoglobin Depletion and Protein Enrichment From Dried Blood Spots

  • Dried blood spots are useful for low volume analyses, and simple collection and transport
  • Protocols suitable for inexpensive whole blood card systems, no need for cell separation
  • Hemoglobin binding >90%, with 30-45 minute spin-filter format
  • Protocols based on ≤10 µl whole blood applied, but suspension format is flexible to most volumes
  • Blood proteins and enzymes are enriched for biomarker and proteomic investigations.
  • Removes hemoglobin from diverse species including human, sheep, bovine, goat, rat, mouse, etc.
  • High throughput and scaleable.

Hemoglobin is a common contaminant from dried whole blood cards and not normally found in serum samples. The HemogloBind™ Blood Card protocol was designed to substantially reduce the presence of hemoglobin and its associated interference with many serum proteins and other analytes.

HemogloBind™ is engineered for a high degree of selectivity and does not cross react with most common serum components, making it an excellent sample prep in numerous applications. These include analytical protocols where optical interference is problematic, such as bilirubin and cholinesterase analysis.

Hemoglobin depletion and Protein Recovery from Dried Blood Spots

Dried Blood Spot (µl) Hemoglobin Present (Based on 200mg Hb per ml blood) (mg) Extraction Buffer (µl) HemogloBind™ Reagent Used (ml) Protein Recovery (µg) Hemoglobin removal (%)
20 4 200 200 400 - 500 > 95%
10 2 100 100 200 - 250 > 95%
5 1 50 50 100 - 125 > 95%
2.5 0.5 25 25 90 - 100 > 95%
Product # of samples processed Item No.
HemogloBind™ Blood Card 10 Dried Whole Blood Spot (7mm hole punch) H0145BC-10
HemogloBind™ Blood Card 50 Dried Whole Blood Spot (7mm hole punch) H0145BC-50
Kit Content 10 Prep 50 Prep Reagent
HemogloBind™ 1 ml 5 ml Supplied
Protein Extraction Buffer (PEB) 1 ml 5 ml Supplied
Spin-X Centrifuge tube filters 10 50 Supplied
Suggested Or Equivalent Supplier of Blood Card: Whatman 903™ Protein Saver cards Not Supplied

Click Here To View HemogloBind Product Sheet

References

Dried Blood Spots Citations:

Michelle R. Robinson, Lei Guo; Raymond J. Gonzalez; Kara M. Pearson; Kevin P. Bateman; Daniel S. Spellman. Differentiating Modes of Drug Induced Liver Injury Using Parallel Reaction Monitoring LC-MS. ASMS Conference 2017 poster report. “Hemoglobin depletion improves PRM panel coverage in DBS and volumetric adsorptive microsampling (VAMS)”.

Hakuna, Lovemore, et al. "A simple assay for glutathione in whole blood." Analyst (2015). (http://pubs.rsc.org/en/content/articlelanding/2015/an/c5an00345h) “…Hb can be removed using a commercial product, HemogloBind™, which can isolate and remove up to 90% of blood Hb."

Other HemogloBind™ Citations:

Red cell lysates
Nguyen, Anthony T., et al. "UBE2O remodels the proteome during terminal erythroid differentiation." Science 357.6350 (2017).

Craig, J. R., et al. "A comparison of the anatomical and gastrointestinal functional development between gilt and sow progeny around birth and weaning." Journal of animal science (2019).

Krishna, Neel K., and Kenji Cunnion. "Derivative Peptide Compounds and Methods of Use." U.S. Patent Application No. 15/192,934. http://www.freepatentsonline.com/y2016/0376322.html

O’Connell, Grant C., et al. "Monocyte-lymphocyte cross-communication via soluble CD163 directly links innate immune system activation and adaptive immune system suppression following ischemic stroke." Scientific reports 7.1 (2017)

Kyoungsook Park, Christopher D. Saudek, and Gerald W. Hart Increased Expression of β-N-Acetylglucosamindase (O-GlcNAcase) in Erythrocytes from Prediabetic and Diabetic Individuals.Diabetes.2010;59(7):1845-50.

Stored Blood Products
Delobel J., Rubin O., Prudent M., Crettaz D., Tissot J.-D., Lion N.(2010) Biomarker Analysis of Stored Blood Products: Emphasis on Pre-Analytical Issues. International Journal of Molecular Sciences. 11(11):4601-4617

Whole Blood.
McGarry, Kevin G., et al. Evaluation of HemogloBind™ treatment for preparation of samples for cholinesterase analysis. (2013). Advances in Bioscience and Biotechnology, 2013, 4, 1020-1023

Red blood cells
Alvarez-Llamas, Gloria, Fernando de la Cuesta, Maria G. Barderas, Irene Zubiri, Maria Posada-Ayala, and Fernando Vivanco. "Characterization of Membrane and Cytosolic Proteins of Erythrocytes." In Vascular Proteomics, pp. 71-80. Humana Press, 2013.

Hikosaka, Keisuke, et al. "Deficiency of Nicotinamide Mononucleotide Adenylyltransferase 3 (Nmnat3) Causes Hemolytic Anemia by Altering the Glycolytic Flow in Mature Erythrocyte" Journal of Biological Chemistry(2014): jbc-M114.

Zihao Wang, Kyoungsook Park, Frank Comer1, Linda C. Hsieh-Wilson, Christopher D. Saudek, Gerald W. Hart. Site-Specific GlcNAcylation of Human Erythrocyte Proteins: Potential Biomarker(s) for Diabetes Mellitus. Diabetes.2008;58, 309-317.