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AlbuVoid™ PLUS - Albumin and IgG Depletion From Serum/Plasma for Proteomics

AlbuVoidâ„¢ PLUS


 
 


Base Price: $305.00


Product Code: NP-AVK


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Description Applications Specifications
 

Albumin and IgG Depletion From Serum/Plasma for Proteomics

  • IgG removal >90% (70-80% of total Immunoglobulins removed)
  • Albumin removal >95%
  • Seamless and simple < 1 hour protocol
  • Low abundance enrichment equivalent to immuno-affinity
  • Disposable, cost-effective, no column regeneration or cross-contamination
  • Works for most species tested including human, sheep, rat, mouse, bovine
  • On-bead protocols improve workflow and efficiency, especially suited to targeted proteomics
  • Suitable for LC-MS, 1 and 2D Gels, ELISAs, Enzyme and other Functional Assays.

The classical plasma proteins generally fall into functional categories, forming the vast majority of the mid-to-high abundance proteome. In serum, these sub-proteomes by mass content are: Albumin 50-60%; Immunoglobulins 10-20%; Transport Proteins (Transferrin, Apo) 5-10%; Complement related Proteins 3-5%; Protease Inhibitors 2-5%; and all others 2-5%. While these sub-proteomes are required for normal body homeostasis, they nevertheless can become dysfunctional during acute-phase and chronic stimuli.

So, depending on the needs of the investigation, it can be valuable to consider that one or more of these categorical sub-proteomes is simply background noise whereby depletion is beneficial. While in other cases, these same categorical sub-proteomes might provide new data and information and consequently, should not be depleted. Different AlbuVoid™, AlbuVoid™ PLUS and AlbuSorb™ PLUS workflows support different proteomic biases as outlined in the following Table.

Products and digest conditions produce different sub-proteome windows of observation. So, depending on the needs of the investigation, it can be valuable to consider that one or more of these categorical sub-proteomes is simply background noise whereby depletion is beneficial. While in other cases, these same categorical sub-proteomes might provide new data and information and consequently, should not be depleted. Categorically the acute-phase sub-proteomes differentiated in disease may vary greatly from those associated with chronic sub-proteomes. So there is great benefit in having options to enrich or deplete one or more of these sub-proteomes.

BSG's Albumin and IgG Removal Kits offer many such options:

  • The 'PLUS' products substantially deplete Immunoglobulins through separations at the protein level.
  • The variable regions of Immunoglobulins are extremely heterogeneous, generating a background noise across the full LC gradient. On-bead digestion (BASP™) with AlbuVoid™ substantially reduces the influence of such Ig peptide features. So in addition to workflow simplicity, BASP™ can be advantageous utilized in targeted proteomic workflows whenever the target proteins do not require strong denaturing conditions.
  • With the exception of Immunoglobulins whereby FASP generates many more spectral features, both strong denaturing conditions (FASP) and on-bead digest (BASP™) conditions produce similar protein profiles.
  • Both Apolipoproteins and heavily glycosylated proteins (i.e., α1-Acid Glycoprotein) bind poorly to AlbuVoid™. For quantitative studies within these classes of proteins, AlbuSorb™ PLUS is recommended.
  • The Complement sub-proteome is especially enriched by AlbuVoid™ PLUS. The digest conditions may bias towards one or more functional sub-populations, likely due to conformational transitions and protein-protein interactions (i.e., Factor Bb, Properdin) that occur upon activation. This needs further investigation.
  • The low abundance sub-proteome is enriched 5+ fold with AlbuVoid™ and 4+ fold with AlbuSorb™ PLUS.
Product Size # Serum Preps Item No.
AlbuVoid™ PLUS Kit 5 preps 5, 25µl Serum samples NP-AVK-05
AlbuVoid™ PLUS Kit 10 preps 10, 25µl Serum samples NP-AVK-10
Click Here For The AlbuVoid™ Plus Product Sheet.
References

Zheng H, Soherwardy A, Roy S, Kuruc M, Avadhani S. AlbuVoid™ Enrichment & Antibody Depletion – Tackling the Challenges of Serum Proteomics Part II. Poster reprint first presented at 26th International Molecular Med TRI-CON, March 10-15, 2019, San Francisco, CA USA

Vialaret, Jerome & Kadi, Sarah & Tiers, Laurent & O Flynn, Robin & Lehmann, Sylvain & Hirtz, Christophe. (2018). Albumin depletion of human serum to improve quantitative clinical proteomics. Current Topics in Peptide & Protein Research 19. 53-62. http://www.researchtrends.net/tia/abstract.asp?in=0&vn=19&tid=26&aid=6192&pub=2018&type=3

Poillet-Perez, Laura, et al. "Autophagy maintains tumour growth through circulating arginine." Nature (2018): 1.

Roy, Swapan, and Matthew Kuruc. "Methods to Monitor the Functional Subproteomes of SERPIN Protease Inhibitors." Functional Proteomics. Humana Press, New York, NY, 2019. 41-54.

Mihara, Keisuke, et al. "Identification of Specific Protein Markers of Rheumatoid Arthritis in Synovial Fluid and Serum." Journal of Hard Tissue Biology 27.1 (2018): 55-58.

David L. Wang, Chuanguang Xiao, Guofeng Fu, Xing Wang and Liang Li. Identification of potential serum biomarkers for breast cancer using a functional proteomics technology. Biomarker Research (2017) 5:11. DOI 10.1186/s40364-017-0092-9

Zheng, H., et al. "AlbuVoid™ coupled to on-bead digestion-tackling the challenges of serum proteomics." J Proteom Bioinformatics 8.9 (2015): 225.

Zheng, H., et al. Poster reprint first presented at 14th Human Proteome Organization World Congress (HUPO 2015), held on September 27 – 30, 2015 entitled “New Proteomic Workflows Combine Albumin Depletion and On Bead Digestion, for Quantitative Cancer Serum”.