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AlbuTrial™ Kit - 1 gram AlbuSorb™ Kit & 5 Preps AlbuVoid Kit



 
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Price: $395.00

Product Code: AVS


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Description Applications Specifications
 

AlbuSorb™
Albumin Depletion From Serum or Plasma
>Removes 30 mg albumin/ml, >90%
>Affinity-type equivalence, virtually no cross-reactivity with other proteins
>Disposable, no column regeneration or cross-contamination
>Economical new surface technology, not based on blue-dye or immuno-affinity chromatography
>Mild binding conditions maintains tertiary structure and simple transfer to secondary analysis
>The flow-through (unbound) fractions retain their enzymatic and biological activity
>Species agnostic

AlbuVoid™
Albumin Depletion Plus Low Abundance Serum Proteome Enrichment
>Albumin voids out in flow-through (unbound) fraction >95%
>Low abundance enrichment better than hexa-peptides or immune-affinity
>Disposable, no column regeneration or cross-contamination
>No low molecular weight or pI bias
>Mild elution conditions maintains tertiary structure and simple transfer to secondary analysis
>The elution fraction retain their enzymatic and biological activity
>Optional
on-bead digestion improves workflow and provides unique proteolytic efficiencies
>Species agnostic

The high abundance of albumin in serum represses the signals for low abundance proteins, thereby interfering with the resolution and sensitivity of many protein profiling techniques. BSG refines the albumin depletion protocols establishing two albumin removal methods using chemical based proteome fractionation systems. The matrices AlbuSorb™ and AlbuVoid™ were tested on human, sheep, bovine, mouse, goat, rat, and calf from serum and plasma using a simple protocol. AlbuSorb™, facilities binding of albumin (>90%) thereby recovering the proteins in the flow-through. AlbuVoid™, by contrast, voids out the majority of albumin (>90%) in the flow-through and wash fractions. In the final elution step the low abundance proteins are enriched. AlbuTrial kit™ includes 1 gm of AlbuSorb™ and 5 Preps of AlbuVoid™ with respective buffers.


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References

AlbuSorb™ References

Cerebrospinal Fluid
Gwenael Pottiez, Pawel Ciborowski. Proteomic Profiling of Cerebrospinal Fluid Expression Profiling In Neuroscience Neuromethods.2012;64:245-270

Synovial fluid
Happonen KE, Fürst CM, Saxne T et al. PRELP protein inhibits the formation of the complement membrane attack complex.Journal of Biological Chemistry.2012;287(11):8092-100

Serum
Holmberg R, Refai E, Höög A.Lowering apolipoprotein CIII delays onset of type 1 diabetes. Proceedings of the National Academy of Sciences.2011;108(26):10685-9.

Tang MX, Ogawa K, Asamoto M. Effects of Nobiletin on PhIP-Induced Prostate and Colon Carcinogenesis in F344 Rats Nutrition and Cancer.2011;63(2):227-33

Holmberg, Rebecka Apolipoprotein CIII and Ljungan virus in diabetes 2010. Doctoral Thesis

Lu Q, Zheng X, McIntosh T Development of different analysis platforms with LC-MS for pharmacokinetic studies of protein drugs. Analytical Chemistry.2009;81(21):8715-23

AlbuVoid™ References

Serum
Swapan Roy, Matthew Kuruc. The Functional Subproteomes of Serpin Protease Inhibitors are Now Open for LC-MS Biomarker Discovery. MOJ Proteomics Bioinform 2016, 3(6): 00106

Serum Profiling Making Mark on Predictive Medicine

Vicki Glaser.Genetic Engineering & Biotechnology News. 2011;31(7):1-55.

Patents
Narain, Niven Rajin, Rangaprasad Sarangarajan, and Vivek K. Vishnudas. "INTERROGATORY CELL-BASED ASSAYS AND USES THEREOF." U.S. Patent No. 20,120,258,874. 11 Oct. 2012.