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AlbuSorb™ - Albumin Depletion Kit



 
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Functional Proteomics
Price: $325.00


Product Code: A185


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Description Applications Specifications
 
  • Removes >90% albumin from 30 mg albumin/ml sample
  • Affinity-type equivalence, virtually no cross-reactivity with other proteins
  • Bind and elute procedure - simply weigh powder, condition the sample, centrifuge and/or filter, and recover the albumin depleted serum
  • Economical surface technology, not based on blue-dye or immuno-affinity chromatography
  • Mild conditions maintain tertiary structure of proteins and simple transfer to secondary analysis
  • The albumin depleted filtrate retains the enzymatic and biological activity
  • Removes albumin from samples such as serum, plasma and from most species including human, mouse, sheep, bovine, goat, rat, and calf
  • The flow through fraction is compatible with LC-MS, activity based protein profiling and proteomic studies.

AlbuSorb™ is an albumin depletion reagent supplied as a kit with necessary buffers. With 25ul of starting sample, the yield of albumin depleted serum protein is 0.1-0.2 milligrams. AlbuSorb™ binds albumin from serum or plasma and is compatible with downstream proteomics methods such as protein array pixelation ,1D and 2D gel electrophoresis, LC/MS, and MALDI-TOF MS. Samples are also prepared for biomarker discovery, toxicological studies for new drugs, enzyme assays, protein profiling using SELDI analysis and cytokines research.

AlbuSorb™ binds to albumin, and serum proteins flow through. Removal of albumin allows enhanced detection of low abundance proteins. AlbuSorb™ comes from a silica-based, separation platform utilizing a new combination of surface microenvironments substituted with low molecular substrates that feature drug-binding motifs. Unlike immuno-affinity, the surfaces utilized are disposable eliminating cycle to cycle variance and cross-contamination.

Biotech Support Group has AlbuVoid™ for albumin depletion plus low abundance serum protein enrichment. Both AlbuSorb and AlbuVoid™ are available in AlbuTrial™ kit™. AlbuTrial™ kit™ includes 1 gm of AlbuSorb and 5 Preps of AlbuVoid™ with respective buffers.



Click here to view AlbuSorb™ Product Sheet
References

Cerebrospinal Fluid
Gwenael Pottiez, Pawel Ciborowski. Proteomic Profiling of Cerebrospinal Fluid Expression Profiling In Neuroscience Neuromethods.2012;64:245-270

Synovial fluid
Happonen KE, Fürst CM, Saxne T et al. PRELP protein inhibits the formation of the complement membrane attack complex.Journal of Biological Chemistry.2012;287(11):8092-100

Serum
Berggren, Per-olof, and Lisa Juntti-berggren. "Methods for treating and/or limiting development of diabetes." U.S. Patent No. 20,170,037,118. 9 Feb. 2017.

Swapan Roy, Matthew Kuruc. The Functional Subproteomes of Serpin Protease Inhibitors are Now Open for LC-MS Biomarker Discovery. MOJ Proteomics Bioinform 2016, 3(6): 00106

Dr.Swapan Roy; Amenah Soherwardy; Ravish Amin; Matthew Kuruc Biotech Support Group LLC, Monmouth Junction, NJ - Application Report. AlbuSorb™ Product Extension combines Albumin and Immunoglobulin Depletion in a Consumable Format

Holmberg R, Refai E, Höög A.Lowering apolipoprotein CIII delays onset of type 1 diabetes. Proceedings of the National Academy of Sciences.2011;108(26):10685-9.

Tang MX, Ogawa K, Asamoto M. Effects of Nobiletin on PhIP-Induced Prostate and Colon Carcinogenesis in F344 Rats Nutrition and Cancer.2011;63(2):227-33

Holmberg, Rebecka Apolipoprotein CIII and Ljungan virus in diabetes 2010. Doctoral Thesis

Lu Q, Zheng X, McIntosh T Development of different analysis platforms with LC-MS for pharmacokinetic studies of protein drugs. Analytical Chemistry.2009;81(21):8715-23

Urine
Zubiri, Irene, et al. "Diabetic nephropathy induces changes in the proteome of human urinary exosomes as revealed by label-free comparative analysis." Journal of proteomics 96 (2014): 92-102.

Patent
Berggren, Per Olaf, Yang, Shao-Nian. 2012. Methods For Treating And/Or Limiting Development Of Diabetes.U.S. Patent 20120328630 Kind Code: A1, filed June 25, 2012, and issued December 27, 2012.