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  Viraffinity™ - Virus and Viral Component Isolation



 
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Price: $405.00

Product Code: V1062


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Description Applications Specifications
 

Viraffinity™

  • Purifies whole infectious non-enveloped virus & non-infectious enveloped virus
  • Complements ultracentrifugation, for viral proteomic enrichment 350X from plasma (Ref: Fragnoud et al, 2016)
  • Isolates antigenic virions, enveloped and non-enveloped
  • Enriches for viral nucleic acids
  • Prepares viral samples for subsequent detection and analysis

Viraffinity™ is a unique water-insoluble elastomeric polyelectrolyte that has been engineered for the capture and recovery of viruses. Applications include: purification of whole infectious non-enveloped virus, virions, viral components, and sample preparation for subsequent detection and analysis. Viraffinity™ is directly added to the sample that is then mixed and centrifuged. The centrifuged pellet contains polyelectrolyte-bound viruses that can then be recovered using a moderately alkaline pH solution. Viraffinity™ is supplied as a suspension reagent ready for use. Simply pipette the suspension into the sample at the appropriate ratio, typically 1 volume of Viraffinity™; to 4 volumes sample. Viraffinity™ is also supplied as the enabling component of the ViraPrep™ application kits.


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References

Patents

Parthasarathy, Ranjani V., Hsi-Chou Liu, Wensheng Xia, Assumpta Bennaars-Eiden, Paul N. Holt, William Bedingham, Christopher R. Kokaisel, and Jeffrey C. Pederson. "Methods for nucleic acid amplification." U.S. Patent Application 12/596,346, filed April 25, 2008.

Parthasarathy, Ranjani, Katya Ericson, and William Bedingham. "Methods for nucleic acid isolation and kits using a microfluidic device and sedimenting reagent." U.S. Patent Application 10/852,022, filed May 24, 2004.

Bedingham, William, et al. "Variable valve apparatus and methods." U.S. Patent No. 8,057,758. 15 Nov. 2011.

Parthasarathy, Ranjani, Katya Ericson, and William Bedingham. "Methods for nucleic acid isolation and kits using solid phase material." U.S. Patent Application No. 10/852,645.

Parthasarathy, Ranjani V., Katya Ericson, and William Bedingham. "Methods for nucleic acid isolation and kits using a microfluidic device and concentration step." U.S. Patent 7,939,249, issued May 10, 2011.

Bedingham, William, Barry Robole, Ranjani Parthasarathy, and Katya Ericson. "Variable Valve Apparatus and Methods." U.S. Patent Application 11/684,656, filed March 12, 2007.

Bedingham, William, et al. "Variable Valve Apparatus and Methods." U.S. Patent Application No. 11/684,656.

European Patent Application EP2305809 Methods for nucleic acid isolation and kits using a microfluidic device and concentration step

Journal Citations

Dengue Virus
Fragnoud, Romain, et al. "Differential proteomic analysis of virus-enriched fractions obtained from plasma pools of patients with dengue fever or severe dengue." BMC infectious diseases 15.1 (2015): 518.

Food Samples

Maurer, John, ed. PCR methods in foods. Springer Science & Business Media, 2006.

D’Souza, D. H. "Update on foodborne viruses: types, concentration and sampling methods." Advances in Microbial Food Safety 2 (2014): 102.

Jaykus, Lee-Ann. "Detection of human enteric viruses in foods." Foodborne Diseases Handbook 2 (2000): 137-163.

Sair, A. I., D. H. D'souza, and L. A. Jaykus. "Human enteric viruses as causes of foodborne disease." Comprehensive reviews in food science and food safety1.2 (2002): 73-89.

Lee, A. "Rapid Detection of Foodborne Viruses from Minimally Processed Foods, poster Australian Society of Microbiology." (2000).

Grohmann, Gary, and Alvin Lee. "Viruses, food and environment." Foodborne microorganisms of public health significance. Sydney: AIFST (1997): 603-620.

Savers, Screen. "EXHIBIT DESCRIPTIONS." Microbial & Comparative Genomics 2.3 (1997): 244-249.

Use of Viraffinity and Multiplex RT-PCR for the dectection of Food borne viruses

Food and Water Samples

Grohmann, Gary, and Alvin Lee. "Viruses, food and environment." Foodborne microorganisms of public health significance. Sydney: AIFST (1997): 603-620.

Isometric Virus

Zeddam, Jean-Louis, et al. "An isometric virus of the potato tuber moth Tecia solanivora (Povolny)(Lepidoptera: Gelechiidae) has a tri-segmented RNA genome." Journal of invertebrate pathology 99.2 (2008): 204-211.

Hantaan Virus

Yu, Hai-Tao, Hong Jiang, Ye Zhang, Xue-Ping Nan, Yu Li, Wei Wang, Wei Jiang et al. "Hantaan virus triggers TLR4-dependent innate immune responses."Viral immunology 25, no. 5 (2012): 387-393.

Wang, Ping-Zhong, Chang-Xing Huang, Ye Zhang, Zhi-Dong Li, Hai-Tao Yu, Ying Zhang, Zhan-Sheng Jia et al. "Analysis of the immune response to Hantaan virus nucleocapsid protein C-terminal-specific CD8+ T cells in patients with Hemorrhagic fever with renal syndrome." Viral immunology 22, no. 4 (2009): 253-260.

Jiang, Hong, Ping-Zhong Wang, Ye Zhang, Zhe Xu, Li Sun, Li-Mei Wang, Chang-Xing Huang et al. "Hantaan virus induces toll-like receptor 4 expression, leading to enhanced production of beta interferon, interleukin-6 and tumor necrosis factor-alpha." Virology 380, no. 1 (2008): 52-59.

Human Enteric Viruses

Leggitt, Paris R., and Lee-ann Jaykus. "Detection methods for human enteric viruses in representative foods." Journal of Food Protection® 63.12 (2000): 1738-1744.

Jaykus, Lee-Ann. "Detection of human enteric viruses in foods." Foodborne Diseases Handbook 2 (2000): 137-163.

Human Mouth Virus

Al-Jarbou, Ahmed. Metagenomic Analysis of the Human Mouth Virus Population and Characterisation of Two Lytic Viruses. Diss. University of Leicester, 2009.

Lepidoptera: Gelechiidae

Zeddam, Jean-Louis, et al. "An isometric virus of the potato tuber moth Tecia solanivora (Povolny)(Lepidoptera: Gelechiidae) has a tri-segmented RNA genome." Journal of invertebrate pathology 99.2 (2008): 204-211.

Serum

Moreau, Isabelle, John Levis, Orla Crosbie, Elizabeth Kenny-Walsh, and Liam J. Fanning et al. "Correlation between pre-treatment quasispecies complexity and treatment outcome in chronic HCV genotype 3a." Virology journal 5, no. 1 (2008): 1.

Abdurahman, Samir. Studies on HIV-1 core assembly. Institutionen för laboratoriemedicin/Department of Laboratory Medicine, 2007.

Human Immunodeficiency Virus Type 1

Abdurahman, Samir, Akos Végvári, Masoud Youssefi, Michael Levi, Stefan Höglund, Elin Andersson, Peter Horal, Bo Svennerholm, Jan Balzarini, Anders Vahlne, et al. "Activity of the small modified amino acid α-hydroxy glycineamide on in vitro and in vivo human immunodeficiency virus type 1 capsid assembly and infectivity." Antimicrobial agents and chemotherapy 52, no. 10 (2008): 3737-3744.

Abdurahman, S., Höglund, S., Höglund, A., & Vahlne, A et al,. (2007). Mutation in the loop C-terminal to the cyclophilin A binding site of HIV-1 capsid protein disrupts proper virus assembly and infectivity. Retrovirology, 4(1), 1.

Abdurahman, Samir, Masoud Youssefi, Stefan Höglund, and Anders Vahlne, et al. "Characterization of the invariable residue 51 mutations of human immunodeficiency virus type 1 capsid protein on in vitro CA assembly and infectivity." Retrovirology 4, no. 1 (2007): 1.

Abdurahman, Samir, Stefan Höglund, Laura Goobar-Larsson, and Anders Vahlne . "Selected amino acid substitutions in the C-terminal region of human immunodeficiency virus type 1 capsid protein affect virus assembly and release." Journal of general virology 85, no. 10 (2004): 2903-2913.

Plasma

Fragnoud, Romain, Marie Flamand, Frederic Reynier, Philippe Buchy, Vasna Duong, Alexandre Pachot, Glaucia Paranhos-Baccala, and Frederic Bedin. "Differential proteomic analysis of virus-enriched fractions obtained from plasma pools of patients with dengue fever or severe dengue." BMC infectious diseases15, no. 1 (2015): 518.

Polio Virus

Ting, W.T. E., E. M. Nielson, and C.C. Tseng. 1997. The use of Viraffinity matrix to concentrate waterborne polioviruses for RT-PCR detection. abstr. Q-169. p.484. In Abstracts of the 97th General Meeting of the American Society for Microbiology 1997, American Society for Microbiology, Washington D.C.

Bacteriophage Lambda DNA

Hitti, J. "Fast and Convenient Purification of Bacteriophage Lambda DNA with Viraffinity™ Matrix, poster Cold Spring Harbor Conference on Genome Mapping & Sequencing." (1997).