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  ProPrep™ BAC Mini Kit

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Price: $255.00

Product Code: PMK

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Description Applications Specifications

ProPrep™ BAC Mini Kit

  • Ideal for mini-prep BAC template preparation from 1.5ml 2xYT cultures.
  • Provides high-quality DNA suitable for automated fluorescent sequencing for small-large insert DNA.
  • Utilizes ProPrep™ strategy which minimizes shearing and improves yield.
  • Adapts easily to robots and automation.

ProPrep™ BAC Mini 100 is a complete purification system based upon the propietary reagent, ProCipitate™, that has been demonstrated to provide high quality DNA suitable for automated fluorescent sequencing of small to large insert DNA. The ProPrep™ strategy is to remove only the contaminants and leave DNA alone. This minimizes shearing, improves yield, and makes ProPrep™ BAC Mini 100 ideal for mini-prep BAC template preparation from 1.5ml 2xYT cultures. The ProPrep™ BAC Mini 100 protocol adapts easily to robots and automation; a simple change and there is no centrifugation required after initial cell concentration.

Some applications ProPrep™ is used for are:

  • Restriction enzyme digestion
  • Human Identity testing
  • PCR amplification
  • Membrane hybridizations (e.g., Southern and dot/slot blots).
  • AFLP, RFLP, RAPD, microsatellite and SNP analyses (for genotyping, fingerprinting, etc.)

Click Here To View ProPrep™ BAC Mini 100 Product Sheet

ProCipitate™, Superior Substitute to Phenol/Chloroform for DNA Isolation & Protein Extraction. by Matt Kuruc February 23, 2015

U.S. Patent Numbers 5,294,681, 5,453,493 and other patents pending.
U.S. Patent Number 5,538,870, Method for Preparing Nucleic Acids For Analysis And Kits Useful Therefore.

Enteric Viruses
Richards, Gary P., and Gail E. Greening. "Detection of enteric viruses in shellfish." Molluscan Shellfish Safety. Springer Netherlands, 2014. 177-183.
Schwab KJ; De Leon R; Sobsey MD. Immunoaffinity concentration and purification of waterborne enteric viruses for detection by reverse transcriptase PCR. Applied and Environmental Microbiology.1996;62(6):2086-94
Schwab KJ, De Leon R, Sobsey MD Concentration and purification of beef extract mock eluates from water samples for the detection of enteroviruses, hepatitis A virus, and Norwalk virus by reverse transcription-PCR. Applied and Environmental Microbiology.1995; 61(2): 531-537
LA Jaykus, R De Leon and MD Sobsey. A virion concentration method for detection of human enteric viruses in oysters by PCR and oligoprobe hybridization Applied and Environmental Microbiology.1996; 62(6): 2074–2080

Mycobacterium Avium Paratuberculosis
Charles G. Thornton; Kerry M. MacLellan; Judith R. Stabel; Christine Carothers; Robert H. Whitlock; Selvin Passen Application of the C18-Carboxypropylbetaine Specimen Processing Method to Recovery of Mycobacterium avium subsp. paratuberculosis from Ruminant Tissue Specimens Journal of Clinical Microbiology.2002;40:5;1783-1790
Zoltan S.; Gyimesi; Ilse H.; Stalis; Janice M.; Miller; Charles O Detection of Mycobacterium avium Subspecies avium in Formalin-Fixed, Paraffin-Embedded Tissues of Captive Exotic Birds Using Polymerase Chain Reaction. Thoen Journal of Zoo and Wildlife Medicine.1999;30:3:348-353
Miller, J.M., et al, Polymerase chain reaction identification of Mycobacterium avium in formalin-fixed, paraffin-embedded animal tissues. Journal of veterinary diagnostic investigation.1999.11:436-440

Paraffin-embedded tissues
Ellingson, J. L. E., et al. "Detection of Mycobacterium avium subspecies paratuberculosis in free-ranging bison (Bison bison) by PCR." Molecular and cellular probes 19.3 (2005): 219-225.

Recombinant Bacterial Colonies
O. Umesh K. Reddy; Alan E. Pepper; Ibrokhim Abdurakhmonov; Sukumar Saha; Johnie N. Jenkins; Thomas Brooks, Yuksel Bolek; Kamal M. El-Zik New Dinucleotide and Trinucleotide Microsatellite Marker Resources The Journal of Cotton Science.2001;5:103-113
Quiniou SM; Katagiri T; Miller NW; Wilson M; Wolters WR; Waldbieser GC Construction and characterization of a BAC library from a gynogenetic channel catfish Ictalurus punctatus. Genetics, selection, evolution : GSE. 2003;35(6):673-83

Bacterial artificial chromosome (BAC) vector
J M Kelley; C E Field; M B Craven; D Bocskai; U J Kim; S D Rounsley; M D Adams. High Throughput Direct End Sequencing of BAC Clones. Nucleic Acids Research.1999.15;27(6):1539-1546

Carcinoma Cell Lines & Bacterial Artificial Chromosomes (BACs)
Alsop, Amber E., Andrew E. Teschendorff, and Paul AW Edwards. "Distribution of breakpoints on chromosome 18 in breast, colorectal, and pancreatic carcinoma cell lines." Cancer genetics and cytogenetics 164.2 (2006): 97-109.

Plasmid Deoxyribonucleic acid (DNA)
G.M. Huang; K. Wang; C.L. Kuo; B. Paeper; L. Hood A High-Throughput Plasmid DNA Preparation Method Analytical Biochemtry.1994;15;223(1):35-8
Robert R. Klein, Daryl T. Morishige, Patricia E. Klein, Jianmin Dong; John E. Mullet. High Throughput BAC DNA Isolation for Physical Map Construction of Sorghum. Plant Molecular Biology Reporter.1988;16(4):651-364

Deoxyribonucleic acid (DNA)
Kozekov ID; Turesky RJ; Alas GR; Harris CM; Harris TM; Rizzo CJ. Formation of Deoxyguanosine Cross-Links from Calf Thymus DNA Treated with Acrolein and 4-Hydroxy-2-nonenal. Chemical Research in Toxicology.2010;23(11):1701-1713
Applied Biosystems User Bulletin. Subject: Sequencing Large DNA Templates.
David C. Bruce; Mark O. Mundt; Kim K. McMurry; Linda J. Meincke; Donna L. Robinson; Norman A. Doggett; Larry L. Deaven. BAC Library End Sequencing in Support of Whole Genome Assemblies DOE Joint Genome Institute and Center for Human Genome Studies, Los Alamos National Laboratory
Dr. Domon, National Agricultural Research Center for Kyushu Okinawa Region, Japan, Extraction of Rush DNA, unpublished personal correspondence, 2004.
DNA Extraction Genetic Diversity as an Indicator of Ecosystem Condition and Sustainability for Regional Assessments of Stream Condition in the Eastern United States

Human Fibroblasts
Stephanie M Cohen, Terrence S Furey, Norman A Doggett, and David G Kaufman. Genome-wide sequence and functional analysis of early replicating DNA in normal human fibroblasts BMC Genomics.2006;7: 301

Norovirus, Adenovirus
Roberto A. Rodríguez, Lauren Thie, Christopher D. Gibbons, Mark D. Sobsey. Reducing the effects of environmental inhibition in quantitative real-time PCR detection of adenovirus and norovirus in recreational seawaters. Journal of Virological Methods;2012:181(S1):43-50
Concentration and purification of beef extract mock eluates from water samples for the detection of enteroviruses, hepatitis A virus, and Norwalk virus by reverse transcription-PCR. Applied and environmental microbiology.1995;61(2):531-7

Krupey, J., et al, 100,000+ PCRs Possible from 10 ml Blood, poster Biotechniques Symposium, 2003.

Human T-cell lymphotrophic virus I-induced adult T-cell leukemia (ATL)
J D Burton; R N Bamford; C Peters; A J Grant; G Kurys; C K Goldman; J Brennan; E Roessler; T A Waldmann. A lymphokine, provisionally designated interleukin T and produced by a human adult T-cell leukemia line, stimulates T-cell proliferation and the induction of lymphokine-activated killer cells Proceedings of the National Academy of Sciences of the United States of America.1994. 91(11): 4935-4939

Zebrafish genomic library of bacterial artificial chromosome (BAC) clones and BAC DNA
McDermott, Brian M., et al. Transgenic labeling of hair cells in the zebrafish acousticolateralis system. Gene Expression Patterns 10.2 (2010): 113-118.