Sample Prep Liquid Biopsy Products Suitable for Proteomic Profiling of a Variety of Body Fluid Sample Types

Diversified Depletion and Enrichment Applications Improve Effectiveness and Ease of Achieving Quality Proteomic Information

By combining two complementary enrichment strategies, Biotech Support Group offers distinctive and diversified technologies to assist researchers achieve quality results by selectively enriching or binding.

Background

Biotech Support Group (BSG) develops cost efficient and reliable proteomic sample prep technologies and methods needed for enhancing proteomics applications in standard healthcare.

One of the four key BSG Advantages is Enrichment/Depletion. As leaders in proteomic sample prep, BSG provides the option of two strategic applications for proteome enrichment in order to get the necessary results: either by selectively binding high abundance or by enriching (choosing not to bind).

The Challenge

Immuno-affinity depletion is often used to remove one or more high abundance proteins from bio-fluids in many proteomic enrichments. However, this strategy commonly comes with limitations such as: high costs, regeneration requirements (which can result in a diminished and inconsistent performance), required marriage of species to antibody, etc.

Considering these limitations, researchers need more reliable ways to enrich.

The Solution

With the emergence of liquid biopsy biomarkers, BSG offers two complementary separation strategies for proteomic researchers to help discover and validate protein biomarkers from bio-fluids in order to achieve the best results.

• One choice is to selectively bind high abundance proteins
• The other choice is to enrich the low abundance proteome.

By using one of these two solutions, researchers will be able to improve efficiency and outcomes regardless of the analytical platform.

BSG Products are an essential toolkit proven for Proteomic Sample Prep for

• Urine
• Blood/Serum/Plasma
• Saliva/Sputum
• Synovial Fluid
• Bronchoalveolar Lavage Fluid (BALF)
• Exosomes/Extracellular Vesicles

The Outcome

Proteomic characterization of the sub-proteomes produced after separations, can be done by a variety of analytical platforms including, functional activity, ELISA, LC-MS, Western blot and cellular response. Through the use of the many products and methods available, researchers will be better equipped to improve outcomes, regardless of the analytical platform.

The products and methods described in the references below will be especially beneficial for discovery investigations essential for expanding proteomics into routine healthcare.

All of our liquid biopsy products are featured on our website.

Related References

For Serum and Synovial Fluid

• For diabetes biomarkers, Cleanascite™ is shown both to improve LC-MS measurements, and validated in accordance with CLIA ’88 guidelines. { doi: 10.1016/j.cca.2016.01.019 }
• Identification of Specific Protein Markers of Rheumatoid Arthritis in Synovial Fluid and Serum
• PRELP protein inhibits the formation of the complement membrane attack complex
• Monoclonal antibody to a cancer-specific and drug-responsive hydroquinone (NADH) oxidase from the sera of cancer patients.

For Whole Blood

• Deleterious Effect of Advanced CKD on Glyoxalase System Activity not Limited to Diabetes Aetiology.
• Evaluation of HemogloBind™ treatment for preparation of samples for cholinesterase analysis.

For Red Cells

• Hypoxia modulates the purine salvage pathway and decreases red blood cell and supernatant levels of hypoxanthine during refrigerated storage.
• Annotating N termini for the Human Proteome Project: N termini and Nα-acetylation status differentiate stable cleaved protein species from degradation remnants in the human erythrocyte proteome.

For Dried Blood Spots/Cards

• A simple assay for glutathione in whole blood

For Bronchoalveolar Lavage Fluid (BALF)

• Proteome Profiling in Lung Injury after Hematopoietic Stem Cell Transplantation

For Urine Exosomes

• Diabetic nephropathy induces changes in the proteome of human urinary exosomes as revealed by label-free comparative analysis

For Saliva/Sputum

• A nanobeads amplified QCM immunosensor for the detection of avian influenza virus H5N1
• Isolation of M. tuberculosis RNA from Sputum
• Influence of different sample preparation strategies on the proteomic identification of stress biomarkers in porcine saliva