Sample Prep – Liquid Biopsy
Proteomic Sample Prep for
- Urine
- Blood/Serum/Plasma
- Saliva/Sputum
- Synovial Fluid
- BALF
- Exosomes
With the emergence of liquid biopsy biomarkers, BSG offers two complementary technologies for proteomic researchers to selectively bind or enrich, in order to achieve the best results. By using one of these two solutions, researchers will be able to improve efficiency and outcomes regardless of the analytical platform. This will be especially beneficial for discovery investigations requiring cost effective and efficient sample prep methods essential for expanding proteomics into routine healthcare.
Urine Protein Enrichment & Concentration
- Linearly scaleable, unlike ultrafiltration
- Alternative to solvent/ alcohol precipitation
- On-bead digestion protocols
- <60 min. bind, wash, elute protocol
- Applicable to
>1 & 2 DE
>LC-MS
>microarrays
- The eluted fractions retain their enzymatic and biological activity
View Details
Selectively Voids Albumin, Binds Low Abundance Proteome
- Albumin voids in flow through, >95%
- <30 minute protocol
- Low abundance enrichment equivalent or better than hexapeptides or antibodies
- On-bead digestion protocols, efficient LC-MS workflows
- Disposable, cost-effective, no column regeneration or cross-contamination
View Details
2DE analysis of AlbuVoid™ treated sheep serum. Samples were reduced, alkylated and total protein normalized. The circled regions indicate the albumin zone. 1: Control. 2: AlbuVoid™ Eluate. Bottom: AlbuVoid™ Eluate silver stain.
Albumin and IgG Depletion From Serum/Plasma for Proteomics
- IgG removal >90% (70-80% of total Immunoglobulins removed)
- Albumin removal >95%
- Seamless and simple < 1 hour protocol
- Low abundance enrichment equivalent to immuno-affinity
- Disposable, cost-effective, no column regeneration or cross-contamination
- Works for most species tested including human, sheep, rat, mouse, bovine
- On-bead protocols improve workflow and efficiency, especially suited to targeted proteomics
- Suitable for LC-MS, 1 and 2D Gels, ELISAs, Enzyme and other Functional Assays.
View Details
20µg of protein loaded on to 10% tris bis gel
A - Serum alone – shows IgG and Albumin regions
B - NuGel™ ProteinA treated serum – shows IgG depletion
C - AlbuVoid™ treated serum – shows Albumin depletion
D - AlbuVoid™ PLUS treated serum – shows IgG and Albumin depletion
Selectively Binds Albumin
- Removes serum albumin >90%
- Economical small ligand surface architecture (not dye-based), bio-affinity performance
- Species agnostic
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Selectively Binds Albumin & Immunoglobulin
- > 85% Albumin, 85% IgG depleted from 25 μl serum
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Quantitative Bias Comparison between
AlbuSorb™ & AlbuSorb™ PLUS
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AlbuSorb™
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AlbuSorb™ PLUS
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Total IG IDs 2+ Spectral Cts
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80
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58
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Total IG % of Spectral Count
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24%
|
9%
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Total Albumin % of Spectral Count
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12%
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15%
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Hemoglobin Depletion For Erythrocyte Proteomics
- Hemoglobin voids in flow-through, applicable to red cells, heavily hemolyzed serum, whole blood and dried blood spot (DBS) card
- Low abundance protein and enzyme enrichment
- Consumable, cost-effective
- Mild elution maintains native structure with retained enzymatic, functional and bioactivities
- Compatible with LC-MS, activity-probe profiling and virtually all proteomic analyses
- Species agnostic
View Details
2DE Comparison.
Red circles indicate the Hemoglobin subunits region. The HemoVoid™ eluate (bottom) has been severely depleted of Hemoglobin. The remainder of the red cell proteins are substantially enriched (visualized) and are better resolved in the HemoVoid™ eluate. Many more proteins are detectable after HemoVoid™ treatment with extensive proteome coverage across both dimensions.
The HemoVoid™ Blood Card kit substantially reduces hemoglobin interference from dried blood spot protein analytes
View Details
Removes Hemoglobin Interference
- Highly specific for hemoglobin binding
- depletion from hemolyzed serum, dura, BALF, and whole blood
- supports biomarker quantitative analysis
View HemogloBind™
View NuGel™ HemogloBind™
Hemoglobin Depletion and Protein Enrichment From Dried Blood Spots
- Dried blood spots are useful for low volume analyses, and simple collection and transport
- Protocols suitable for inexpensive whole blood card systems, no need for cell separation
- Hemoglobin binding >90%, with 30-45 minute spin-filter format
- Protocols based on ≤10 µl whole blood applied, but suspension format is flexible to most volumes
- Blood proteins and enzymes are enriched for biomarker and proteomic investigations.
- Removes hemoglobin from diverse species including human, sheep, bovine, goat, rat, mouse, etc.
- High throughput easicly scalable.
View Details
Lipid Adsorption & Clarification
- Effectively replaces chlorinated/fluorinated hydrocarbons (eg. freon)
- Workflows for antibodies, proteins, nucleic acids, proteoglycans, and most serum analytes
- A high binding capacity for lipids with minimal cross-reactivity with proteins and nucleic acids
View Details
Cleanascite™ is supplied as a suspension reagent. Simply add, mix and centrifuge in a 10 minute protocol.
Glycoprotein Enrichment Using Phenyl Boronic Acid
- Enriches heterogeneous sets of glycoprotein’s, N-linked & O-linked
- Consumable, no column regeneration
- Species and tissue agnostic
- Sorbitol elution; compatible with functional assays, electrophoresis and LC-MS
- Binds biomolecules containing 1,2 cis-diol groups
- Chemically derived, ideal for glyco-proteomic applications
- NuGel™ polymer coating, porous silica based
- Supplied as bead only (dry powder) or as kit (includes all binding and elution buffers)
View NuGel™ PBA
View NuGel™ PBA Kit
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Sample Type
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%Glycoprotein (Sorbitol Elution)
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|
Mouse Plasma
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33
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Rat Serum
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44
|
|
Sheep Serum
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18
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Bovine Serum
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40
|
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Bovine Brain Homogenate
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9
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Different heterogeneous sets of glycoproteins are observed from 4 different mammalian
Gel Key:
- A: Mouse Plasma Eluate
- B: Sheep Serum Eluate
- C: Bovine Serum Eluate
- D: Rat Serum Eluate
Our BSG Advantage
Cost-effective, not derived from biologicals
- No specialized instruments or HPLC
- Economical surface chemistries, not derived from biologicals
- No regeneration, so no prep to prep variability
- Simple, fast microfuge bind/wash/elute protocols
Efficient workflows, quality LC-MS/MS data
- Simple, reproducible workflows
- Equivalent or better than in-solution digestion
- Seamless to LC-MS, no desalting or C18 separations
- Unique proteolytic efficiencies
Diverse strategies, species agnostic
- Products support strategies for both enrichment of low abundance proteomes, or depletion of high abundance proteins
- Species agnostic not derived from biologicals
Maintained throughout all separations
- Mild buffer conditions maintains native structure with retained enzymatic, functional & bio-activities
- Supports enzyme biomarker assays
- Functional & Chemical Proteomics
- Structural & activity-probe Proteomics
- Top-down & ArrayBridge PEP Proteomics
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