Sample Prep – Liquid Biopsy

Proteomic Sample Prep for

  • Urine
  • Blood/Serum/Plasma
  • Saliva/Sputum
  • Synovial Fluid
  • BALF
  • Exosomes

With the emergence of liquid biopsy biomarkers, BSG offers two complementary technologies for proteomic researchers to selectively bind or enrich, in order to achieve the best results. By using one of these two solutions, researchers will be able to improve efficiency and outcomes regardless of the analytical platform. This will be especially beneficial for discovery investigations requiring cost effective and efficient sample prep methods essential for expanding proteomics into routine healthcare.


UPCK™ Kit

Urine Protein Enrichment & Concentration

  • Linearly scaleable, unlike ultrafiltration
  • Alternative to solvent/ alcohol precipitation
  • On-bead digestion protocols
  • <60 min. bind, wash, elute protocol
  • Applicable to
    >1 & 2 DE
    >LC-MS
    >microarrays
  • The eluted fractions retain their enzymatic and biological activity
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Concentrated Enriched Urinary Proteins

AlbuVoid™

Selectively Voids Albumin, Binds Low Abundance Proteome

  • Albumin voids in flow through, >95%
  • <30 minute protocol
  • Low abundance enrichment equivalent or better than hexapeptides or antibodies
  • On-bead digestion protocols, efficient LC-MS workflows
  • Disposable, cost-effective, no column regeneration or cross-contamination
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2DE analysis of AlbuVoid™ treated sheep serum

2DE analysis of AlbuVoid™ treated sheep serum. Samples were reduced, alkylated and total protein normalized. The circled regions indicate the albumin zone. 1: Control. 2: AlbuVoid™ Eluate. Bottom: AlbuVoid™ Eluate silver stain.

AlbuSorb™

Selectively Binds Albumin

  • Removes serum albumin >90%
  • Economical small ligand surface architecture (not dye-based), bio-affinity performance
  • Species agnostic
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AlbuSorb™ PLUS

Selectively Binds Albumin & Immunoglobulin

  • > 85% Albumin, 85% IgG depleted from 25 μl serum
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Quantitative Bias Comparison between AlbuSorb™ & AlbuSorb™ PLUS

AlbuSorb™ AlbuSorb™ PLUS
Total IG IDs 2+ Spectral Cts 80 58
Total IG % of Spectral Count 24% 9%
Total Albumin % of Spectral Count 12% 15%
Total Proteins IDs

HemoVoid™

Hemoglobin Depletion For Erythrocyte Proteomics

  • Hemoglobin voids in flow-through, applicable to red cells, heavily hemolyzed serum, whole blood and dried blood spot (DBS) card
  • Low abundance protein and enzyme enrichment
  • Consumable, cost-effective
  • Mild elution maintains native structure with retained enzymatic, functional and bioactivities
  • Compatible with LC-MS, activity-probe profiling and virtually all proteomic analyses
  • Species agnostic
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2DE Comparison

2DE Comparison.
Red circles indicate the Hemoglobin subunits region. The HemoVoid™ eluate (bottom) has been severely depleted of Hemoglobin. The remainder of the red cell proteins are substantially enriched (visualized) and are better resolved in the HemoVoid™ eluate. Many more proteins are detectable after HemoVoid™ treatment with extensive proteome coverage across both dimensions.

HemoVoid™ Blood Card Kit

The HemoVoid™ Blood Card kit substantially reduces hemoglobin interference from dried blood spot protein analytes

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Albumin and Hemoglobin Regions

HemogloBind™
and
NuGel™ HemogloBind™

Removes Hemoglobin Interference

  • Highly specific for hemoglobin binding
  • depletion from hemolyzed serum, dura, BALF, and whole blood
  • supports biomarker quantitative analysis
View HemogloBind™ View NuGel™ HemogloBind™
HemogloBind™

Cleanascite™

Lipid Adsorption & Clarification

  • Effectively replaces chlorinated/fluorinated hydrocarbons (eg. freon)
  • Workflows for antibodies, proteins, nucleic acids, proteoglycans, and most serum analytes
  • A high binding capacity for lipids with minimal cross-reactivity with proteins and nucleic acids
View Details
Cleanascite™ is supplied as a suspension reagent.

Cleanascite™ is supplied as a suspension reagent. Simply add, mix and centrifuge in a 10 minute protocol.


Notable References

Plasma/Serum Protein Biomarkers

For diabetes biomarkers, Cleanascite™ is shown both to improve LC-MS measurements, and validated in accordance with CLIA ’88 guidelines. { doi: 10.1016/j.cca.2016.01.019 }


Monoclonal antibody to a cancer-specific and drug-responsive hydroquinone (NADH) oxidase from the sera of cancer patients.


For saliva/sputum/tracheal swab

A nanobeads amplified QCM immunosensor for the detection of avian influenza virus H5N1


Isolation of M. tuberculosis RNA from Sputum


NuGel™ PBA
and
NuGel™ PBA Kit

Glycoprotein Enrichment Using Phenyl Boronic Acid

  • Enriches heterogeneous sets of glycoprotein’s, N-linked & O-linked
  • Consumable, no column regeneration
  • Species and tissue agnostic
  • Sorbitol elution; compatible with functional assays, electrophoresis and LC-MS
  • Binds biomolecules containing 1,2 cis-diol groups
  • Chemically derived, ideal for glyco-proteomic applications
  • NuGel™ polymer coating, porous silica based
  • Supplied as bead only (dry powder) or as kit (includes all binding and elution buffers)
View NuGel™ PBA View NuGel™ PBA Kit
Sample Type %Glycoprotein (Sorbitol Elution)
Mouse Plasma 33
Rat Serum 44
Sheep Serum 18
Bovine Serum 40
Bovine Brain Homogenate 9
NuGel™ Phenyl Boronic Acid

Different heterogeneous sets of glycoproteins are observed from 4 different mammalian
Gel Key:

  • A: Mouse Plasma Eluate
  • B: Sheep Serum Eluate
  • C: Bovine Serum Eluate
  • D: Rat Serum Eluate

Our BSG Advantage


Consumable

Cost-effective, not derived from biologicals

Cost-effective, not derived from biologicals

  • No specialized instruments or HPLC
  • Economical surface chemistries, not derived from biologicals
  • No regeneration, so no prep to prep variability
  • Simple, fast microfuge bind/wash/elute protocols

On-Bead Digestion

Efficient workflows, quality LC-MS/MS data

Efficient workflows, quality LC-MS/MS data

  • Simple, reproducible workflows
  • Equivalent or better than in-solution digestion
  • Seamless to LC-MS, no desalting or C18 separations
  • Unique proteolytic efficiencies

Enrichment/Depletion

Diverse strategies, species agnostic

Diverse strategies, species agnostic

  • Products support strategies for both enrichment of low abundance proteomes, or depletion of high abundance proteins
  • Species agnostic not derived from biologicals

Functional Integrity

Maintained throughout all separations

Maintained throughout all separation

  • Mild buffer conditions maintains native structure with retained enzymatic, functional & bio-activities
  • Supports enzyme biomarker assays
  • Functional & Chemical Proteomics
  • Structural & activity-probe Proteomics
  • Top-down & ArrayBridge PEP Proteomics