Home > Products >

AlbuSorb™ - Albumin Depletion Kit

AlbuSorb Albumin Depletion Kit


 
 


Functional Proteomics
Base Price: $395.00


Product Code: A185


Size (A185):


Qty:
Description
 

Albumin Depletion From Serum or Plasma

  • Removes 30 mg albumin/ml, >90%

  • Affinity-type equivalence, virtually no cross-reactivity with other proteins

  • Disposable, no column regeneration or cross-contamination

  • Economical new surface technology, not based on blue-dye or immuno-affinity chromatography

  • Mild binding conditions maintains tertiary structure and simple transfer to secondary analysis

  • The flow-through (unbound) fractions retain their enzymatic and biological activity

  • Removes albumin from most species including human, sheep, bovine, mouse, goat, rat, and calf.

  • Validated in the automation compatible high-throughput DPX Technologies XTR tip format


Poly-electrolytes are polymers with repeating units of stationary charges. AlbuSorb™ comes from a class of solid-phase, or bead-based, elastomeric poly-electrolytic beads that bind proteins through an empirically derived chemistry combining elements of polymer composition, cross-linking architecture and charge properties. As with bio-polymers like DNA and Heparin, governing their reactivity is the spatial presentation of the electrostatic groups along a flexible polymer chain.

Unlike immuno-affinity, the surfaces utilized are disposable eliminating cycle to cycle variance and cross-contamination. AlbuSorb™ is supplied as a dry powder. Simply weigh, centrifuge and/or filter, and recover the albumin depleted serum in the supernatant.


Click here to view AlbuSorb™ Product Sheet
References


Whole blood, serum and plasma samples

DPX Technologies and Biotech Support Group Announce In-Market Partnership for High Throughput Proteomic Sample Prep


Blood Serum

Leiden University Medical Center and Biotech Support Group Report Progress on Stromal Conditioning Biomarkers in Cancer

Plasma
Valladolid-Acebes, Ismael, et al. " Lowering apolipoprotein CIII protects against high-fat diet–induced metabolic derangements. " Science Advances 7.11 (2021): eabc2931.


Serum

Zheng H, Soherwardy A, Roy S, Kuruc M, Avadhani S. AlbuVoid™ Enrichment & Antibody Depletion – Tackling the Challenges of Serum Proteomics Part II. Poster reprint first presented at 26th International Molecular Med TRI-CON, March 10-15, 2019, San Francisco, CA USA

Roy, Swapan, and Matthew Kuruc. "Methods to Monitor the Functional Subproteomes of SERPIN Protease Inhibitors." Functional Proteomics. Humana Press, New York, NY, 2019. 41-54.

Berggren, Per-olof, and Lisa Juntti-berggren. "Methods for treating and/or limiting development of diabetes." U.S. Patent No. 20,170,037,118. 9 Feb. 2017.

Swapan Roy, Matthew Kuruc. The Functional Subproteomes of Serpin Protease Inhibitors are Now Open for LC-MS Biomarker Discovery. MOJ Proteomics Bioinform 2016, 3(6): 00106


Dr.Swapan Roy; Amenah Soherwardy; Ravish Amin; Matthew Kuruc Biotech Support Group LLC, Monmouth Junction, NJ - Application Report. AlbuSorb™ Product Extension combines Albumin and Immunoglobulin Depletion in a Consumable Format


Holmberg R, Refai E, Höög A.Lowering apolipoprotein CIII delays onset of type 1 diabetes. Proceedings of the National Academy of Sciences.2011;108(26):10685-9.


Tang MX, Ogawa K, Asamoto M. Effects of Nobiletin on PhIP-Induced Prostate and Colon Carcinogenesis in F344 Rats Nutrition and Cancer.2011;63(2):227-33


Holmberg, Rebecka Apolipoprotein CIII and Ljungan virus in diabetes 2010. Doctoral Thesis


Lu Q, Zheng X, McIntosh T Development of different analysis platforms with LC-MS for pharmacokinetic studies of protein drugs. Analytical Chemistry.2009;81(21):8715-23


Serum exosomes

Chettimada, Sukrutha, et al. " Exosome markers associated with immune activation and oxidative stress in HIV patients on antiretroviral therapy
." Scientific Reports 8.1 (2018): 7227.


Serum or plasma

Åvall, Karin. "In vivo and in vitro studies of apolipoprotein CIII in diabetes." (2017).

Cerebrospinal Fluid

Gwenael Pottiez, Pawel Ciborowski. Proteomic Profiling of Cerebrospinal Fluid Expression Profiling In Neuroscience Neuromethods.2012;64:245-270


Synovial fluid

Happonen KE, Fürst CM, Saxne T et al. PRELP protein inhibits the formation of the complement membrane attack complex.Journal of Biological Chemistry.2012;287(11):8092-100

Urine
Zubiri, Irene, et al. "Diabetic nephropathy induces changes in the proteome of human urinary exosomes as revealed by label-free comparative analysis." Journal of proteomics 96 (2014): 92-102.


Patent

Berggren, Per Olaf, Yang, Shao-Nian. 2012. Methods For Treating And/Or Limiting Development Of Diabetes.U.S. Patent 20120328630 Kind Code: A1, filed June 25, 2012, and issued December 27, 2012.