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AlbuSorb™ - Albumin Depletion Kit

AlbuSorb Albumin Depletion Kit
 
 


Functional Proteomics
Base Price: $495.00

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$495.00


Product Code: A185


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Description Safety Data Sheet
 

AlbuSorb™- Albumin Depletion From Serum or Plasma

  • Removes 30 mg albumin/ml, >90%
  • Affinity-type equivalence, virtually no cross-reactivity with other proteins
  • Disposable, no column regeneration or cross-contamination
  • Economical new surface technology, not based on blue-dye or immuno-affinity chromatography
  • Mild binding conditions maintains tertiary structure and simple transfer to secondary analysis
  • The flow-through (unbound) fractions retain their enzymatic and biological activity
  • >Removes albumin from most species including human, sheep, bovine, mouse, goat, rat, and calf.
  • Validated in the automation compatible high-throughput DPX Technologies XTR tip format.

Poly-electrolytes are polymers with repeating units of stationary charges. AlbuSorb™ comes from a class of solid-phase, or bead-based, elastomeric poly-electrolytic beads that bind proteins through an empirically derived chemistry combining elements of polymer composition, cross-linking architecture and charge properties. As with bio-polymers like DNA and Heparin, governing their reactivity is the spatial presentation of the electrostatic groups along a flexible polymer chain.

Unlike immuno-affinity, the surfaces utilized are disposable eliminating cycle to cycle variance and cross-contamination. AlbuSorb™ is supplied as a dry powder. Simply weigh, centrifuge and/or filter, and recover the albumin depleted serum in the supernatant.

Click here to view AlbuSorb™ Product Sheet

Kit includes:

Items Item No Item No Reagent
AlbuSorb™ A185-1 (1 grams) A185-6 (6 grams) Supplied
Binding Buffer BB1,
pH 7.5
30 ml 180 ml Supplied
Typical Performance AlbuSorb™ AlbuSorb™ PLUS

Serum Sample Volume

25 µl

25 µl

Albumin Removal

>90%

>85%

IgG Removal

-

>85%

Recovered Protein Mass

500-600 µg

(Albumin depleted)

400-500 µg

(Albumin + Ig depleted)

LC-MS/MS unique proteins

600-800

600-800

References


Selected References


Serum/Plasma

Fraser DD, Roy S, Kuruc M, Quintero M, Van Nynatten LR, Cepinskas G, Zheng H, Soherwardy A and Roy D (2025) Functional mass spectrometry indicates anti-protease and complement activity increase with COVID-19 severity. Exp. Biol. Med. 250:10308. doi: 10.3389/ebm.2025.10308. Investigations on some innate immunity proteins can yield misleading information, as investigators most often rely on static measurements and assume a direct correlation to function. However, function is often not directly proportional to protein abundance, and mechanistic pathways are interconnected and under constant regulatory control. In this study, we used functional mass spectrometry to measure anti-protease and complement activity in plasma obtained from coronavirus disease 2019 (COVID-19) patients. The target peptides were selected to report specific regions of the protein(s) that infer functionality. Taken together, tryptic peptides selected from functional regions thus can serve as surrogates for reporting functional activity. For Albumin & IgG depletion, the article states “The clarified serum was loaded onto the conditioned AlbuSorb™ PLUS beads…”, prior to LC-MS/MS analysis.


Grantz, Jillian M., et al. "The platelet and plasma proteome and targeted lipidome in postpartum dairy cows with elevated systemic inflammation." Scientific Reports 14.1 (2024): 31240. Unregulated, systemic inflammation negatively impacts health and production in dairy cows. Soluble mediators and platelets have been studied for their expansive role in mediating inflammation. The objectives of this study were to compare the plasma oxylipin and endocannabinoid profiles, and the platelet and plasma proteomic profiles of healthy cows to cows experiencing elevated systemic inflammation as indicated by plasma haptoglobin (Hp) concentrations. The article states “Plasma samples were depleted of albumin using AlbuSorb™ Plus Albumin and IgG depletion Kit (APK285-20, BioTech Support Group, Monmouth Junction, NJ), following the manufacturer’s instructions.” In total, 1,016 proteins were identified in the plasma proteome. Proteomic outputs from plasma revealed 24 proteins to be different between high and low-inflammation groups, including proteins involved in autophagy and immune mediation.

Jordan, Christine KI, et al. "Symbiotic Firmicutes establish mutualism with the host via innate tolerance and resistance to control systemic immunity." Cell Host & Microbe (2023). The intestinal microbiota regulates immunity across organ systems. Which symbionts control systemic immunity, the mechanisms they use, and how they avoid widespread inflammatory damage are unclear. Despite systemic penetration of Firmicutes, immune homeostasis is maintained through feedback control whereby macrophages clear polymeric glycoconjugates from peripheral tissues. Smaller glycoconjugates evading this clearance mechanism are tolerated through sequestration by albumin, which acts as an inflammatory buffer constraining their immunological impact. To determine this, the article states, “AlbuSorb (Biotech Support Group) was used to deplete albumin from mouse plasma as per manufacturer instructions.” “We found that complete human and murine plasma activated our NOD2, TLR2, and TLR4 reporter cells and that post-albumin depletion, plasma from both species activated reporter cells less potently, indicating that albumin sequesters circulating cell wall glycoconjugates.”


Valladolid-Acebes, Ismael, et al. "Lowering apolipoprotein CIII protects against high-fat diet–induced metabolic derangements." Science Advances 7.11 (2021): eabc2931. Increased levels of apolipoprotein CIII (apoCIII), result in obesity-related metabolic derangements. Using mice, the researchers investigated mechanistically whether lowering or preventing high-fat diet (HFD)– induced increase in apoCIII, protects against the detrimental metabolic consequences. For Western blotting determination of circulating apoCIII, the article states, “plasma was albumin depleted using AlbuSorb according to the manufacturer’s protocol (Biotech Support Group LLC) and resuspended in 0.1% (v/v) trifluoroacetic acid.”


Nelson K, Wilkinson, S. et al., High resolution accurate mass spectrometry-based proteomics in ecotoxicology: SWATH-MS to detect differentially expressed plasma proteins in the amphibian toxicological model Xenopus laevis. Poster: Conference: PRIMO20, May 2019. The poster describes methods to identified and quantitated plasma proteins that are sex specific in X. laevis adults, stating “Plasma samples from individuals of all ages: albumin-depleted (AlbuSorb™ Biotech Support Group)…”


Holmberg R, Refai E, Höög A. Lowering apolipoprotein CIII delays onset of type 1 diabetes. Proceedings of the National Academy of Sciences.2011;108(26):10685-9.


Tang MX, Ogawa K, Asamoto M. Effects of Nobiletin on PhIP-Induced Prostate and Colon Carcinogenesis in F344 Rats Nutrition and Cancer.2011;63(2):227-33


Lu Q, Zheng X, McIntosh T. Development of different analysis platforms with LC-MS for pharmacokinetic studies of protein drugs. Analytical Chemistry.2009;81(21):8715-23


Berggren, Per Olaf, Yang, Shao-Nian. 2012. Methods For Treating And/Or Limiting Development Of Diabetes.U.S. Patent 20120328630 Kind Code: A1, filed June 25, 2012, and issued December 27, 2012


Exosome Proteomics

Chettimada, Sukrutha, et al. "Exosome markers associated with immune activation and oxidative stress in HIV patients on antiretroviral therapy." Scientific Reports 8.1 (2018): 7227. For LC-MS/MS proteomics, the article states “To obtain purified exosome fractions for proteomic analysis,… albumin was depleted with 2 rounds of albumin depletion using AlbuSorb™ - Albumin Depletion Kit (Biotech Support Group)”.

Zubiri, Irene, et al. Diabetic nephropathy induces changes in the proteome of human urinary exosomes as revealed by label-free comparative analysis. Journal of Proteomics (2013).


Synovial fluid

Happonen KE, Fürst CM, Saxne T et al
. PRELP protein inhibits the formation of the complement membrane attack complex. Journal of Biological Chemistry.2012;287(11):8092-100.
PRELP is a 58-kDa proteoglycan found in a variety of extracellular matrices, is released into the synovial fluid where it may interact with components of the complement system. For Western Blot of Synovial Fluid, albumin was depleted using AlbuSorb (Biotech Support Group).


Cell Culture
Matsuzawa-Ishimoto, Yu, et al.
"The γδ IEL effector API5 masks genetic susceptibility to Paneth cell death." Nature (2022): 1-8. Loss of Paneth cells and their antimicrobial granules compromises the intestinal epithelial barrier and is associated with Crohn’s disease. Non-classical lymphoid cells, broadly referred to as intraepithelial lymphocytes (IELs), intercalate the intestinal epithelium. The identification of mediators that coordinate crosstalk between specific IEL and epithelial subsets could provide insight into intestinal barrier mechanisms in health and disease. Here we show that the subset of IELs that express γ and δ T cell receptor subunits (γδ IELs) promotes the viability of Paneth cells deficient in the Crohn’s disease susceptibility gene ATG16L1. For immunoblotting of intestinal organoid supernatants, the article states “…albumin was depleted with AlbuSorb… according to the manufacturer’s instructions”.


For a full list of Albumin Removal references, visit:

https://www.biotechsupportgroup.com/References-s/138.htm#albumin-depletion



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