Cleanascite™ Reference Applications
Omental Conditioned Medium
David, et al. "
Fatty Acids Promote Protumoral Macrophage Polarization via a
RhoA-YAP1 Signaling Pathway in the Ovarian Cancer Microenvironment
marrow neutrophil cells
RPMI containing 0.5% FBS or with 10% FBS
Ly, et al. "
trafficking to the site of infection requires Cpt1a-dependent fatty
(FBS) and BSA
Lifeng, et al. "
Glycolysis-Driven Lipogenesis Supports Proliferation and Survival of
Human Pulmonary Arterial Smooth Muscle Cells in Pulmonary
Detect Tuberculosis Virulence Factors In Serum
Wenshu, et al. "
disease diagnosis using pathogen-derived tryptic peptides from
Today 45 (2022): 101515.
Marwah M., et al.
acids secreted from head and neck cancer induce M2‐like
of Leukocyte Biology
Flori, E., Mastrofrancesco, A., Mosca, S., Ottaviani, M., Briganti, S., Cardinali, G., Filoni, A., Cameli, N., Zaccarini, M., Zouboulis, C.C., Picardo, M.,Sebocytes
contribute to melasma onset
Omental Conditioned Medium (OCM)
Yang, X. U. A. N., et al. " SCD1/FADS2 fatty acid desaturases equipoise lipid metabolic activity and redox-driven ferroptosis in ascites-derived ovarian cancer cells ." (2021).
Conza, Giusy, et al.
reshuffling of lipid composition on the endoplasmic reticulum
membrane sustains macrophage survival and pro-tumorigenic
precipitate of lipids
collecting, and storing ascites
Spring Harbor Protocols
Xueyu, et al.
Silencing of miR-33b Promotes Peritoneal Metastases of Ovarian Cancer
by Modulating the TAK1/FASN/CPT1A/NF-κB Axis."
Hsin-Yi, et al. "
abrogation of S6K2 maps lipid homeostasis as a survival vulnerability
in MAPKi-resistant NRASmut melanoma.
Lisa, et al. "
pollen induces Toll-like receptor 4-dependent dendritic cell
activation favoring T cell responses.
Fetal Bovine Serum
Miller-Rhodes, Patrick, and Harris A. Gelbard."The Cell Culture Environment Regulates the Transcription Factor MafB in BV-2 Microglia." Matters 7.1 (2021): e202010000001.
Léna, Anne-Claire Groo, and Aurélie Malzert-Fréon. "
Physicochemical Methods to Functionalize, Purify, and Characterize
Surface-Decorated Lipid-Based Nanocarriers
Molecular Pharmaceutics (2020).
Edward A. "
and Storing Hybridoma Tissue Culture Supernatants
Spring Harbor Protocols 2020.10 (2020): pdb-prot103317.
Elia, et al. "
from Pieris brassicae eggs activate an immune response in
" eLife 9 (2020): e60293.
(Omental conditioned medium) culture
DW, Yung MM, Chan Y-Sang, Xuan Y, Yang H, Xu D, Zhan J-Biao, Chan KK,
Ng T-Bun, Ngan HY,
protein from Momordica charantia is therapeutic and has synergic
activity with cisplatin against ovarian cancer in vivo by altering
metabolism and inducing ferroptosis
, Pharmacological Research
Ana P., et al. "
accumulation of methylmalonic acid promotes tumour progression
Nature (2020): 1-5.
Haiping, et al. "
metabolic adaptation supports regulatory T cell survival and function
regulatory T cells (Treg cells) to counteract immunosuppressive
features of the tumor microenvironment (TME) is an attractive
strategy for cancer treatment. However, systemic impairment of their
suppressive function limits its therapeutic potential. Elucidating
approaches that specifically disrupt intratumoral Treg cells is
direly needed for cancer immunotherapy. The use of Cleanascite™
helped demonstrate that intratumoral Treg cells increase lipid
metabolism and CD36 expression. The article states “cancer
cell-conditioned medium … was treated with Cleanascite™ reagent
(Biotech Support Group) before Treg cell culture at a volume ratio of
1:5 according to the manufacturer’s instructions.”
Rain R., et al. "
of lipid metabolism with a metabolic inhibitor cocktail eradicates
peritoneal metastases in ovarian cancer cells
cancer is an intra-abdominal tumor in which the presence of ascites
facilitates metastatic dissemination, and is associated with poor
prognosis. However, the significance of metabolic alterations in
ovarian cancer cells in the ascites microenvironment remains unclear.
In this study, the authors investigated whether reprogramming of
lipid metabolism in ovarian cancer cells could modulate cell
viability and aggressiveness. The article states: ”To determine
whether fatty acids in OCM are the primary energy source, fatty acids
from OCM was first removed by Cleanascite™ Lipid Removal Reagent...
Then, XTT cell proliferation assays showed that the growth rate of
ovarian cancer cells was remarkably reduced in cells cultured in
Cleanascite ™-treated OCM (Fig.
Likewise, co-treatment with Cleanascite™ and OCM significantly
attenuated the increased cell migration and invasion capacities of
ES-2 and SKOV3 cells (Fig.
). These findings suggest that the fatty acid-enriched OCM
provides as an energy source for supporting tumor growth and
aggressiveness of ovarian cancer cells.
Hong-Jai, et al. "
effect of humoral milieu on the viral DNA and surface antigen
expression of hepatitis B virus (HBV) in vitro.
& Cellular Toxicology
if humoral milieu such as serum or culture media, and its
constituents, and pH would regulate the viral DNA and surface antigen
expression of HBV
analysis showed decreased level of HBV DNA and surface antigen
expression in human and mouse serum.
article states “
To evaluate the lipid exposure status within
lipid bilayer, Cleanascite™ (Biotech Support Group) was added to
HBV mixtures in the human serum, mouse serum, or DMEM, and the HBsAg
and HBV DNA were evaluated. … we examined the virus-lipid
interaction in non-host milieu, and compared the interaction between
in host and non-host milieu. The levels of HBsAg and HBV DNA were
significantly decreased with lipid removal by Cleanascite™ in mouse
serum rather than human serum
Neil T., et al. "
reticulum stress is transmissible in vitro between cells of the
central nervous system
protein folding and trafficking are common pathological events in
neurodegenerative diseases that result in the toxic accumulation of
misfolded proteins within the lumen of the endoplasmic reticulum
(ER). The cell
role of sustained unfolded protein response activation under
physiological and pathological states in the central nervous system
(CNS) remains to be elucidated.
authors studied the characteristics of a mediator secreted by
stressed astrocytes and neurons
To determine if the mediator was a lipid associated factor, t
article states “…
μl of Cleanascite™ slurry was added to 1 ml of conditioned medium
and incubated at RT with end-over-end mixing for 1 h followed by
Response & Extracellular vesicle clearance
Doan C., et al. "
vesicles from bone marrow-derived mesenchymal stromal cells support
ex vivo survival of human antibody secreting cells
of extracellular vesicles
study investigated the role of extracellular vesicles (EVs) in
antibody secreting cell survival and IgG secretion. The article
understand whether disrupting the lipid plasma membrane that upsets
the integrity of the EVs would compromise the survival activity of
either the non-irradiated or irradiated secretomes, …We then
cultured ASC with conventional media (vehicle), secretome from
irradiated MSC, or secretome from irradiated MSC that had been
pretreated with the lipid-disrupting agent Cleanascite™, which is
known not to alter protein functionality [
Cleanascite™-treatment of the secretome dramatically reduced ASC
functional survival, … Similar reductions were also noted with the
secretome of non-irradiated MSC when treated with Cleanascite™ …
These results demonstrate that lipid-membrane bodies, such as EVs,
could mediate important ASC survival factors within the MSC
Mette, et al.
serum and bile protein markers predict primary sclerosing cholangitis
disease severity and prognosis
of hepatology 66.6 (2017): 1214-1222.
Natalija, et al. "
integrated approach for comparative proteomic analysis of human bile
reveals overexpressed cancer-associated proteins in malignant biliary
et Biophysica Acta (BBA)-Proteins and Proteomics
Elisa, et al. "
of bile and serum mucin5AC in cholangiocarcinoma: Diagnostic
performance and biologic significance
Annarita, et al. "
carcinoembryonic cell adhesion molecule 6 (CEAM6) as a biomarker of
malignant biliary stenoses.
et Biophysica Acta (BBA)-Proteins and Proteomics
W, Ai KX, Yuan Z, Huang XY, Zhang HZ.
Expression of S100A8 in Malignant and Benign Gallbladder
diseases and sciences. 2012.
RA, Lima AA, Ziolli RL, Campos RC.
report of metalloproteinases in fish bile and their potential as
bioindicators regarding environmental contamination
A, Dumonceau JM, Frossard JL.
Analysis of Human Bile from Malignant Biliary Stenosis Induced by
of Proteome Research.2009; 8(1):159-69
Bo, Zheng Jian-wei, Wang Jian-ming, et al.
and preliminary analysis of a 2-D human biliary map
Journal of Hepatobiliary Surgery.2007
B, Dong JQ, Chen YJ et al
electrophoresis for comparative proteomic analysis of human bile
Hepatobiliary & pancreatic diseases international.2007
TZ, Bunkenborg J, Gronborg M et al
Proteomic Analysis of Human Bile
and Cellular Proteomics.2004;3:715-728
Wade, Michelle Cummins, Anthony Keyburn and Tamsyn M.
and detection of microRNA from the egg of chickens
Research Notes 2016 9:283.
of Noncirculating Biological Fluids to Improve Discovery of
Clinically Relevant Protein Biomarkers
for Biomarker Discovery
Humana Press, New York, NY, 2019. 23-37.
proteomic biomarker discovery, it is necessary to bridge the gap
between basic and applied research by complying with clinical
This chapter provides key suggestions for improving the discovery of
clinically relevant protein biomarkers from body fluids. The chapter
states : ”If the elimination of lipids…is necessary, the sample
can by treated with lipid removal
T Clark, Paul J Russell, and Steven Westwood.
without impact: a case study in clinical assay failure due to
Bioanalysis; 2012: 4,(12):1421-1428
J., He, L., Lanza, G., Tollefsen, D. and Wickline,
perfluorocarbon nanoparticles provide a novel strategy for the
treatment and magnetic resonance imaging of acute thrombosis
Journal of Thrombosis and Haemostasis.2011;9:1292-1300
D, Schmidt O, Liliensiek AK.
preservation and DNA extraction methods for intestinal microbial
diversity analysis in earthworms
of Microbiological Methods.2009;76(3):226-33
AM, Moore EE, Masuno T et al
Mesenteric Lymph Blunts the Pulmonary Inflammatory Response to
of Surgical Research.2006;136(S2):166-171
T, Mackie IJ, Machin SJ et al.
levels of beta 2 glycoprotein I antigen and beta 2 glycoprotein I
binding antibodies are associated with a history of thromboembolic
complications in patients with SLE and primary antiphospholipid
journal of rheumatology.1995 Nov;34(11):1031-6
RF; Brandao C; Maia M; Arosa FA.
blood cells release factors with growth and survival bioactivities
for normal and leukemic T cells
Immunology and Cell Biology.2011;89(1):111-21
D, Wang J, Wang R, Li Y.
nanobeads amplified QCM immunosensor for the detection of avian
influenza virus H5N1
Biosensors and Bioelectronics.2011;26(S10):4146-4154
LM, Shinnick TM.
exploration of the drug action of capreomycin on Mycobacterium
tuberculosis using Affymetrix oligonucleotide GeneChips
LM, Shinnick TM.
analysis of intergenic regions of mycobacterium tuberculosis H37Rv
using affymetrix genechips
EURASIP journal on bioinformatics & systems biology.2007:23054
and Cell Culture
MS, Schroder C, Hoheisel JD.
conditions for proteomic profiling of mammalian tissue and cell
extracts with antibody microarrays
RK, Kharlamov A, Jones SC.
of brain endothelial nitric oxide synthase concentration in rat and
E. Danielle, et al. "
glucagon signaling reveals hepatic α cell axis and role for
L-glutamine in α cell proliferation.
glucagon action lowers blood glucose and may be useful
therapeutically for diabetes. However, interrupted glucagon signaling
leads to α cell proliferation. In this article, the authors wanted
to determine which factors affected α cell proliferation.
article states “
lipid removal, whole mouse serum was treated with Cleanascite™
reagent (Biotech Support Group, Monmouth Junction, NJ) prior to islet
culture at a 1:1 ratio according to the vendor’s protocol. Lipid
removal was validated by HPLC to remove 99% of all phopsholipids,
cholesterols, and triglycerides….”.
Steven W., et al. "
high-throughput mass spectrometry assay to simultaneously measure
intact insulin and C-peptide
." Clinica Chimica Acta
(2016). Cleanascite™ is shown both to improve LC-MS
measurements, and validated in accordance with CLIA ’88 guidelines.
John A., et al. "
autoantibodies as blood biomarkers for detection of early stage
Rohun U., et al. "
Liposomes Establish A Sustained Localized Anticlotting Barrier
Against Acute Thrombosis.
Molecular pharmaceutics (2013).
M, Caruthers S, Hu G.
SPECT-CT/MR Molecular Imaging of Angiogenesis in the Vx2
JD, Langley RS, Johnston KL.
Lipoprotein Stimulates Innate and Adaptive Immunity through Toll-like
Receptors 2 and 6 to Induce Disease Manifestations of Filariasis
Journal of Biological Chemistry.2009;284:22364-22378
JB, DesJardin LE, MacNeil J. et al
of Mycobacterium tuberculosis mannosyltransferase pimB reduces the
cell wall lipoarabinomannan and lipomannan content and increases the
rate of bacterial-induced human
N, Chueh PJ, Kim C et al
antibody to a cancer-specific and drug-responsive hydroquinone (NADH)
oxidase from the sera of cancer patients.
Immunology, Immunotherapy. 2002;51(3):121-9
S, Beenhouwer DO, Feldmesser M et al.
G Monoclonal Antibodies to Cryptococcus neoformans Protect Mice
Deficient in Complement Component C3 Infect.
AR, Morrill We, Pope V.
Removal from Human Serum Samples
and diagnostic laboratory immunology.2000;7(2):197-199
of M. tuberculosis RNA from Sputum
in Molecular Medicine.2001;48:133-139
DO, Shapiro S, Feldmesser M et al.
Th1 and Th2 Cytokines Affect the Ability of Monoclonal Antibodies To
Protect Mice against Cryptococcus neoformans Infection
and immunity.2001;69: 6445-6455
LE, Perkins MD, Wolski K et al.
of Sputum Mycobacterium tuberculosis Messenger RNA as a Surrogate for
Response to Chemotherapy
journal of respiratory and critical care medicine.1999;160(1):203-10
Dov, et al. "
for quantitation of insulin and c-peptide
U.S. Patent Application No. 15/942,188.
inventors describe methods for diagnosing or prognosing insulin
resistance in diabetic and pre-diabetic patients, the method
comprising determining the amount of insulin and C-peptide in a
sample. Mass spectrometric methods are described for detecting and
quantifying insulin and C-peptide in a biological sample utilizing
enrichment and/or purification methods coupled with tandem mass
spectrometric or high resolution/high accuracy mass spectrometric
techniques. The application states “
some embodiments, serum is delipidated prior to quantitation by mass
spectrometry. … In some embodiments, the delipidation reagent is
Yoichiro, Kakuta Shigeru, Suzuki, Shunsuke - United States Patent
and Pharmaceutical Composition for Treatment of Intestinal Disease
John A. United States Patent: 20120107841.
Diagnostic Method, Biomarker and Kit for Early Detection and Staging
of Alzheimer's Disease
James D et al. United States Patent: 20030170757.
antibodies specific for neoplasia-specific NADH: disulfide reductase
Morre, NM McCarty, D Morre et al United States Patent:
antibodies specific for neoplasia-specific NADH: disulfide reductase
C. Jones. United States Patent: 7999084.
and methods for reducing matrix effects
Research (Cholesterol Removal From Human Serum)
Thierry, Monica Bologa, Robert Hopfer, David Neveu, Branda Hu,
Xiaohua Sheng, Nicolas Corde, Catherine Pouzet,
Gloria Zimmerman, and
and immunogenicity of the pneumococcal pneumolysin derivative PlyD1
in a single-antigen protein vaccine candidate in adults.
globin protein expression and heme biosynthesis improve production of
human hemoglobin in Saccharomyces cerevisiae
Metabolic Engineering (2013).