ProPrep™ BAC Mini Kit

Product Code: PMK

ProPrep™ BAC Mini Kit

Ideal for mini-prep BAC template preparation from 1.5ml 2xYT cultures.
Provides high-quality DNA suitable for automated fluorescent sequencing from small to large insert DNA.
Utilizes the unique phenol/chloroform replacement ProCipitate™ which minimizes shearing and improves yield.
Used throughout the Human Genome Sequencing Project.

ProPrep™ BAC Mini 100 is a complete purification system based upon the propietary reagent, ProCipitate™, that has been demonstrated to provide high quality DNA suitable for automated fluorescent sequencing of small to large insert DNA. The ProPrep™ strategy is to remove only the contaminants and leave DNA alone. This minimizes shearing, improves yield, and makes ProPrep™ BAC Mini 100 ideal for mini-prep BAC template preparation from 1.5ml 2xYT cultures. The ProPrep™ BAC Mini 100 protocol adapts easily to robots and automation.

Click Here To View ProPrep™ BAC Mini 100 Product Sheet

Review
ProCipitate™, Superior Substitute to Phenol/Chloroform for DNA Isolation & Protein Extraction. by Matt Kuruc February 23, 2015

Patents

U.S. Patent Numbers 5,294,681, 5,453,493 and other patents pending.

U.S. Patent Number 5,538,870, Method for Preparing Nucleic Acids For Analysis And Kits Useful Therefore.

Enteric Viruses

Richards, Gary P., and Gail E. Greening. "Detection of enteric viruses in shellfish." Molluscan Shellfish Safety. Springer Netherlands, 2014. 177-183.

Schwab KJ; De Leon R; Sobsey MD. Immunoaffinity concentration and purification of waterborne enteric viruses for detection by reverse transcriptase PCR. Applied and Environmental Microbiology.1996;62(6):2086-94

Schwab KJ, De Leon R, Sobsey MD Concentration and purification of beef extract mock eluates from water samples for the detection of enteroviruses, hepatitis A virus, and Norwalk virus by reverse transcription-PCR. Applied and Environmental Microbiology.1995; 61(2): 531-537

LA Jaykus, R De Leon and MD Sobsey. A virion concentration method for detection of human enteric viruses in oysters by PCR and oligoprobe hybridization Applied and Environmental Microbiology.1996; 62(6): 2074–2080

Mycobacterium Avium Paratuberculosis

Charles G. Thornton; Kerry M. MacLellan; Judith R. Stabel; Christine Carothers; Robert H. Whitlock; Selvin Passen Application of the C18-Carboxypropylbetaine Specimen Processing Method to Recovery of Mycobacterium avium subsp. paratuberculosis from Ruminant Tissue Specimens Journal of Clinical Microbiology.2002;40:5;1783-1790

Zoltan S.; Gyimesi; Ilse H.; Stalis; Janice M.; Miller; Charles O Detection of Mycobacterium avium Subspecies avium in Formalin-Fixed, Paraffin-Embedded Tissues of Captive Exotic Birds Using Polymerase Chain Reaction. Thoen Journal of Zoo and Wildlife Medicine.1999;30:3:348-353

Miller, J.M., et al, Polymerase chain reaction identification of Mycobacterium avium in formalin-fixed, paraffin-embedded animal tissues. Journal of veterinary diagnostic investigation.1999.11:436-440

Paraffin-embedded tissues

Ellingson, J. L. E., et al. "Detection of Mycobacterium avium subspecies paratuberculosis in free-ranging bison (Bison bison) by PCR." Molecular and cellular probes 19.3 (2005): 219-225.

Recombinant Bacterial Colonies

O. Umesh K. Reddy; Alan E. Pepper; Ibrokhim Abdurakhmonov; Sukumar Saha; Johnie N. Jenkins; Thomas Brooks, Yuksel Bolek; Kamal M. El-Zik New Dinucleotide and Trinucleotide Microsatellite Marker Resources The Journal of Cotton Science.2001;5:103-113

Quiniou SM; Katagiri T; Miller NW; Wilson M; Wolters WR; Waldbieser GC Construction and characterization of a BAC library from a gynogenetic channel catfish Ictalurus punctatus. Genetics, selection, evolution : GSE. 2003;35(6):673-83

Bacterial artificial chromosome (BAC) vector

J M Kelley; C E Field; M B Craven; D Bocskai; U J Kim; S D Rounsley; M D Adams. High Throughput Direct End Sequencing of BAC Clones. Nucleic Acids Research.1999.15;27(6):1539-1546

Carcinoma Cell Lines & Bacterial Artificial Chromosomes (BACs)

Alsop, Amber E., Andrew E. Teschendorff, and Paul AW Edwards. "Distribution of breakpoints on chromosome 18 in breast, colorectal, and pancreatic carcinoma cell lines." Cancer genetics and cytogenetics 164.2 (2006): 97-109.

Plasmid Deoxyribonucleic acid (DNA)
G.M. Huang; K. Wang; C.L. Kuo; B. Paeper; L. Hood A High-Throughput Plasmid DNA Preparation Method Analytical Biochemtry.1994;15;223(1):35-8

Robert R. Klein, Daryl T. Morishige, Patricia E. Klein, Jianmin Dong; John E. Mullet. High Throughput BAC DNA Isolation for Physical Map Construction of Sorghum. Plant Molecular Biology Reporter.1988;16(4):651-364

Deoxyribonucleic acid (DNA)
Kozekov ID; Turesky RJ; Alas GR; Harris CM; Harris TM; Rizzo CJ. Formation of Deoxyguanosine Cross-Links from Calf Thymus DNA Treated with Acrolein and 4-Hydroxy-2-nonenal. Chemical Research in Toxicology.2010;23(11):1701-1713
Applied Biosystems User Bulletin. Subject: Sequencing Large DNA Templates.

David C. Bruce; Mark O. Mundt; Kim K. McMurry; Linda J. Meincke; Donna L. Robinson; Norman A. Doggett; Larry L. Deaven. BAC Library End Sequencing in Support of Whole Genome Assemblies DOE Joint Genome Institute and Center for Human Genome Studies, Los Alamos National Laboratory

Dr. Domon, National Agricultural Research Center for Kyushu Okinawa Region, Japan, Extraction of Rush DNA, unpublished personal correspondence, 2004.

DNA Extraction Genetic Diversity as an Indicator of Ecosystem Condition and Sustainability for Regional Assessments of Stream Condition in the Eastern United States

Human Fibroblasts
Stephanie M Cohen, Terrence S Furey, Norman A Doggett, and David G Kaufman. Genome-wide sequence and functional analysis of early replicating DNA in normal human fibroblasts BMC Genomics.2006;7: 301

Norovirus, Adenovirus
Roberto A. Rodríguez, Lauren Thie, Christopher D. Gibbons, Mark D. Sobsey. Reducing the effects of environmental inhibition in quantitative real-time PCR detection of adenovirus and norovirus in recreational seawaters. Journal of Virological Methods;2012:181(S1):43-50

Concentration and purification of beef extract mock eluates from water samples for the detection of enteroviruses, hepatitis A virus, and Norwalk virus by reverse transcription-PCR. Applied and environmental microbiology.1995;61(2):531-7

Blood
Krupey, J., et al, 100,000+ PCRs Possible from 10 ml Blood, poster Biotechniques Symposium, 2003.

Human T-cell lymphotrophic virus I-induced adult T-cell leukemia (ATL)
J D Burton; R N Bamford; C Peters; A J Grant; G Kurys; C K Goldman; J Brennan; E Roessler; T A Waldmann. A lymphokine, provisionally designated interleukin T and produced by a human adult T-cell leukemia line, stimulates T-cell proliferation and the induction of lymphokine-activated killer cells Proceedings of the National Academy of Sciences of the United States of America.1994. 91(11): 4935-4939

Zebrafish genomic library of bacterial artificial chromosome (BAC) clones and BAC DNA
McDermott, Brian M., et al. Transgenic labeling of hair cells in the zebrafish acousticolateralis system. Gene Expression Patterns 10.2 (2010): 113-118.

Do BSG's products work for all species?

Yes, as our products are not derived from antibodies, they are species agnostic, and have applied to humans, mice, rats, guinea pigs, goats, cows, pigs, even frogs and fish.

Can you supply bulk material only (from the kit-based products) for large volume orders?

Yes, we can adjust all of our kit products, to supply only the beads. For large orders, whether kit or bulk material, we provide special discounted quotations. Please inquire.

Can the protocol be scaled to accommodate different amounts of either volume or mass?

Yes, we provide general guidelines for use, but the protocols can usually be adjusted to accommodate either larger or small starting samples, or larger or smaller starting concentrations. We recommend first contacting us for guidance in adjusting the protocol.

Can the products be adapted to high throughput formats and automation?

Although we do not have an off-the-shelf product for 96-well formats currently, we have ongoing collaborations that can provide such formats. Please inquire.

Are the products compatible with detergents?

This has not been fully investigated, but we do not recommend use of SDS. Low concentrations, 0.1 – 0.5% of non-ionic detergents have been used with some of our products, but to what extent they alter separation performance, is unknown. If non-ionic detergents are to be used, generally we recommend that they be of no greater than 0.1% before applying to the beads. So for tissue lysates for example at higher %, dilute the sample before application.

What is the stability and shelf life of the product?

The products have a shelf life typically 6 months to 1 year. We ship at room temperature, but recommend storage at 4°C upon receipt. Do not freeze.

Do the products contain any biologically derived material?

All of our products are derived chemically, not biologically, with one exception. The "PLUS" products contain recombinant Protein A.

How can I order?

We can take on-line cart orders, which accept North American credit cards, Visa & MasterCard. It does not however, accept other international credit cards. If you would like to order direct, contact us at [email protected]. In the US, distributors Fisher Scientific and VWR can be used to order all products, even those not listed on their sites, just provide them with our catalog #. A full list of international distributors is listed on How to Order | Biotech Support Group LLC.

Kit includes:

Items Required	Quantity	ProPrep Mini BAC 100	Storage
Alcohol	-	Not Supplied	-
TE1 Resuspension Buffer	12 ml	Supplied	Room Temp. (or 4°C after RNAse addition)
RNase Cocktail	1ml	Supplied	-20°C
AL2 Alkaline Lysis Buffer (If precipitate forms, solubilize by placing bottle in warm water)	12 ml	supplied	Room Temp.
NB3 Neutralization Buffer	12 ml	Supplied	Room Temp.
ProCipitate	10 ml	Supplied	4°C (best if used before date on label)
Wide Bore Pipette Tips	-	Not Supplied	-

Performance:

MSDS:
MSDS-ProPrep™ RNAse Cocktail

Product Code	Name	Base Price	Qty
Product Code:PMK-100	ProPrep BAC Mini Kit - 100, 2 mL cultures	Base Price:$385.00

ProPrep™ BAC Mini Kit

Description

References

FAQ