NuGel™ Poly-Amine

Specialized product line for ligand immobilization
Suitable for LCMS, ELISA, Western Blot, Functional Analysis
Retains function and bio-activity

Product Code: NPAM

NuGel™ Poly-Amine For Immobilization

Non-specific sites are virtually eliminated by a polymer coating
Stable across a wide pH range 2 – 10
1000Å, 50µm Silica suitable for LC and batch processes
Applications include: Enzyme immobilization, separation and purification of biopolymers, immobilization of proteins & ligands – monoclonal antibodies, hormones, peptides, haptens, drugs, etc.

Silica has been an industry standard as an advantageous matrix suitable for high performance liquid chromatography. With NuGel™, non-specific sites have been virtually eliminated making it an ideal support for affinity purification. Through a proprietary polymer coating, Silica is crosslinked forming a reactive Poly-Epoxy functionality stable across a wide pH range (pH 2 to 10). From this foundational chemistry, all of the NuGel™ affinity products are derived.

NuGel™ Poly-Amino Technical Data

NuGel™ Poly-Amino is a derivative of NuGel™ polyhydroxy affinity support. This affinity support contains amino groups at the end of hydrophilic spacer arms and is used to couple ligands containing carboxyl, aldehyde, anhydride and epoxy groups.

Technical Data
Spacer Arm	Polymerized hydrophilic carbon chain
Porosity	1000Å
Average Particle Size	50um
Substitution Level	100-200 uEq/gm of amine groups

Special Features:

Couples ligands containing free carboxyl groups in the presence of a carbodiimide.
Couples ligands containing free aldehyde, anhydride and epoxy groups.
Couples non-polar ligands in organic solvents.
pH stable from 2 to 9.

Click Here To View NuGel™ Poly-Amine Product Protocol

NuGel™ Related References

Patents

Monoclonal antibodies directed to the cytotoxic lymphocyte maturation factor European Patent EP0790255

Purification of immunoglobulins using affinity chromatography and peptide US 2006/0153834 A1

Affinity

Chaumet, A., Castella, S., Gasmi, L., Fradin, A., Clodic, G., Bolbach, G., ... & Larcher, J. C. (2013). Proteomic analysis of interleukin enhancer binding factor 3 (Ilf3) and nuclear factor 90 (NF90) interactome. Biochimie, 95(6), 1146-1157.

Dermot Walls, Robert McGrath and Sinéad T.Loughran A Digest of Protein Purification. Methods Molecular Biology. Volume 681: 3-23 (2011)

Ehrlich, G. K., Michel, H., Chokshi, H. P. and Malick, A. W. Affinity purification and characterization of an anti-PEG IgM. Journal of Molecular Recognition, 22: 99–103 (2009).

Nassal,M., Skamel, C., Vogel, M., Kratz, P. A., Stehle, T., Wallich, R., &Simon, M. M. (2008). Development of hepatitis B virus capsids into a whole-chain protein antigen display platform: new particulate Lyme disease vaccines. InternationalJournal of Medical Microbiology, 298(1-2),135-142.

A sensitive and high-throughput assay to detect low-abundance proteins in serum Hongtao Zhang, Xin Cheng, Mark Richter & Mark I Greene. Nature Medicine 12, 473 - 477 (2006)

Kramer,A., & Schneider-Mergener, J. (2002). Transformation of a L-peptide epitope into a D-peptide analog. In PeptidesFrontiers of Peptide Science (pp.769-770). Springer, Dordrecht

Expression and folding of an antibody fragment selected in vivo for high expression levels in Escherichia coli cytoplasm. Research in Microbiology Volume 153, Issue 7, September 2002, Pages 469-474

Identification of model peptides as affinity ligands for the purification of humanized monoclonal antibodies by means of phage displayJournal of Biochemical and Biophysical Methods Volume 49, Issues 1-3.2001

George K. Ehrlich, Pascal Bailon, Wolfgang Berthold. Phage Display Technology - Identification of Peptides as Model Ligands for Affinity Chromatography Affinity Chromatography Methods in Molecular Biology, 2000, Volume 147, 209-220

A Digest of Protein Purification and partial amino acid sequence of a 28 kDa cyclophilin-like component of the rat liver sigma receptor. Life Sciences , Volume 55, Issue 8, 1994.

Nachman, M., Azad, A. R. M. and Bailon, P. (1992), Efficient recovery of recombinant proteins using membrane-based immunoaffinity chromatography (MIC). Biotechnology and Bioengineering, 40: 564–571.

Kinetic aspects of membrane-based immunoaffinity chromatography. Journal of Chromatography A Volume 597, Issues 1-2, 24 April 1992, Pages 167-172

Identification of model peptides as affinity ligands for the purification of humanized monoclonal antibodies by means of phage display. Methods in Molecular Biology, 2000, Volume 147, 209-220

Membrane-based receptor affinity chromatography. Journal of Chromatography A Volume 597, Issues 1-2, 24 April 1992, Pages 155-166 9th International Symposium on Affinity Chromatography and Biological Recognition

Ion Exchange

Levin W Protein Purification of recombinant human secretory phospholipase A2 (group II) produced in long-term immobilized cell culture. Expr Purif 1992 Feb;3(1):27-35.

Do BSG's products work for all species?

Yes, as our products are not derived from antibodies, they are species agnostic, and have applied to humans, mice, rats, guinea pigs, goats, cows, pigs, even frogs and fish.

Can you supply bulk material only (from the kit-based products) for large volume orders?

Yes, we can adjust all of our kit products, to supply only the beads. For large orders, whether kit or bulk material, we provide special discounted quotations. Please inquire.

Can the protocol be scaled to accommodate different amounts of either volume or mass?

Yes, we provide general guidelines for use, but the protocols can usually be adjusted to accommodate either larger or small starting samples, or larger or smaller starting concentrations. We recommend first contacting us for guidance in adjusting the protocol.

Can the products be adapted to high throughput formats and automation?

Although we do not have an off-the-shelf product for 96-well formats currently, we have ongoing collaborations that can provide such formats. Please inquire.

Are the products compatible with detergents?

This has not been fully investigated, but we do not recommend use of SDS. Low concentrations, 0.1 – 0.5% of non-ionic detergents have been used with some of our products, but to what extent they alter separation performance, is unknown. If non-ionic detergents are to be used, generally we recommend that they be of no greater than 0.1% before applying to the beads. So for tissue lysates for example at higher %, dilute the sample before application.

What is the stability and shelf life of the product?

The products have a shelf life typically 6 months to 1 year. We ship at room temperature, but recommend storage at 4°C upon receipt. Do not freeze.

Do the products contain any biologically derived material?

All of our products are derived chemically, not biologically, with one exception. The "PLUS" products contain recombinant Protein A.

How can I order?

We can take on-line cart orders, which accept North American credit cards, Visa & MasterCard. It does not however, accept other international credit cards. If you would like to order direct, contact us at [email protected]. In the US, distributors Fisher Scientific and VWR can be used to order all products, even those not listed on their sites, just provide them with our catalog #. A full list of international distributors is listed on How to Order | Biotech Support Group LLC.

Kit includes:

Technical Data
Spacer Arm	Polymerized hydrophilic carbon chain
Porosity	1000Å
Average Particle Size	50um
Substitution Level	100-200 uEq/gm of Amino groups

Special Features:

Covalently couples ligands containing free carboxyl groups in the presence of a carbodiimide.
Covalently couples ligands containing free aldehyde, anhydride and epoxy groups.
Covalently couples non-polar ligands in organic solvents.
pH stable from 2 to 9.

NuGel™ Poly-Amine, Catalog Order # NPAM-25

Product Code	Name	Base Price	Qty
Product Code:NPAM-20	NuGelâ„¢ Poly-Amine - 20 Grams	Base Price:$750.00

NuGel™ Poly-Amine

Description

References

FAQ