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ProCipitate™

ProCipitate™


 
 


Base Price: $465.00


Product Code: P0050


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Description
 

ProCipitate™ - Superior Substitute to Phenol/Chloroform for Hemoglobin & Protein Removal, Isolation of DNA/RNA

  • Removes protein contaminants & leaves DNA & RNA soluble and unreacted
  • Ideal for applications when the alternative kits don’t fit, or are not optimal
  • Improves yield of DNA over alternative bind and elute systems
  • Adaptable to any sample size and can be automated
  • Pathogen and infectious disease testing
  • Tissue and paraffin-embedded tissues

The ProCipitate™ strategy is opposite to common prep strategies, as instead of binding nucleic acids, the proteins and especially - Hemoglobin, is efficiently depleted with no interaction with the soluble nucleic acids.

ProCipitate™ is a unique protein binding reagent developed from patented solid-phase polyelectrolytes. These elastomeric polymer suspension reagents are prepared in an extended state due to strong electrostatic repulsion of the repeating polymeric acid groups. Upon interaction with proteins, salt bridges form and the resultant complex collapses to a lower energy state, expelling water much like dehydration processes taking place within solvent precipitations. In this way, ProCipitate™ is characteristic of phenol/chloroform separation, a long established benchmark for nucleic acid isolation. However, ProCipitate™ is non-volatile, non-hazardous, and has the additional benefits of solid-phase suspensions; that is - the adaptability to filtration and automation. ProCipitate™ has been on the market for over 20 years being used throughout the Human Genome Sequencing Project. It is routinely used for improving the yield consistency and protein depleted quality of DNA & RNA.



Click Here To View ProCipitate™ Product Sheet
References

Industrial crude porcine heparin (ICPH)

Huang, Q., Yao, C. Y., Chen, B., Wang, F., Huang, J. F., Zhang, X., & Fu, W. L. (2006). Species-specific identification by inhibitor-controlled PCR of ruminant components contaminating industrial crude porcine heparin. Molecular and cellular probes, 20(3-4), 250-258.


Human skeletal bone

Smith, S. E., Hayes, M. G., Cabana, G. S., Huff, C., Coltrain, J. B., & O'rourke, D. H. (2009). Inferring population continuity versus replacement with aDNA: A cautionary tale from the Aleutian Islands. Human biology, 81(4), 407-426.


Review

ProCipitate™, Superior Substitute to Phenol/Chloroform for DNA Isolation & Protein Extraction. by Matt Kuruc February 23, 2015


Patents

U.S. Patent Numbers 5,294,681, 5,453,493 and other patents pending.

U.S. Patent Number 5,538,870, Method for Preparing Nucleic Acids For Analysis And Kits Useful Therefore.

Enteric Viruses

Richards, Gary P., and Gail E. Greening. "Detection of enteric viruses in shellfish." Molluscan Shellfish Safety. Springer Netherlands, 2014. 177-183.

Schwab KJ; De Leon R; Sobsey MD. Immunoaffinity concentration and purification of waterborne enteric viruses for detection by reverse transcriptase PCR. Applied and Environmental Microbiology.1996;62(6):2086-94

Schwab KJ, De Leon R, Sobsey MD Concentration and purification of beef extract mock eluates from water samples for the detection of enteroviruses, hepatitis A virus, and Norwalk virus by reverse transcription-PCR. Applied and Environmental Microbiology.1995; 61(2): 531-537

LA Jaykus, R De Leon and MD Sobsey. A virion concentration method for detection of human enteric viruses in oysters by PCR and oligoprobe hybridization Applied and Environmental Microbiology.1996; 62(6): 2074–2080

Mycobacterium Avium Paratuberculosis

Charles G. Thornton; Kerry M. MacLellan; Judith R. Stabel; Christine Carothers; Robert H. Whitlock; Selvin Passen Application of the C18-Carboxypropylbetaine Specimen Processing Method to Recovery of Mycobacterium avium subsp. paratuberculosis from Ruminant Tissue Specimens Journal of Clinical Microbiology.40:5;1783-1790

Zoltan S.; Gyimesi; Ilse H.; Stalis; Janice M.; Miller; Charles O Detection of Mycobacterium avium Subspecies avium in Formalin-Fixed, Paraffin-Embedded Tissues of Captive Exotic Birds Using Polymerase Chain Reaction . Thoen Journal of Zoo and Wildlife Medicine.1999;30:3:348-353

Miller, J.M., et al, Polymerase chain reaction identification of Mycobacterium avium in formalin-fixed, paraffin-embedded animal tissues. Journal of veterinary diagnostic investigation.1999.11:436-440


Paraffin-embedded tissues


Ellingson, J. L. E., et al. "Detection of Mycobacterium avium subspecies paratuberculosis in free-ranging bison (Bison bison) by PCR." Molecular and cellular probes 19.3 (2005): 219-225.


Recombinant Bacterial Colonies

O. Umesh K. Reddy; Alan E. Pepper; Ibrokhim Abdurakhmonov; Sukumar Saha; Johnie N. Jenkins; Thomas Brooks, Yuksel Bolek; Kamal M. El-Zik New Dinucleotide and Trinucleotide Microsatellite Marker Resources The Journal of Cotton Science.2001;5:103-113

Quiniou SM; Katagiri T; Miller NW; Wilson M; Wolters WR; Waldbieser GC Construction and characterization of a BAC library from a gynogenetic channel catfish Ictalurus punctatus. Genetics, selection, evolution : GSE. 2003;35(6):673-83

Bacterial artificial chromosome (BAC) vector

J M Kelley; C E Field; M B Craven; D Bocskai; U J Kim; S D Rounsley; M D Adams. High Throughput Direct End Sequencing of BAC Clones. Nucleic Acids Research.1999.15;27(6):1539-1546

Carcinoma Cell Lines & Bacterial Artificial Chromosomes (BACs)


Alsop, Amber E., Andrew E. Teschendorff, and Paul AW Edwards. "Distribution of breakpoints on chromosome 18 in breast, colorectal, and pancreatic carcinoma cell lines." Cancer genetics and cytogenetics 164.2 (2006): 97-109.

Plasmid Deoxyribonucleic acid (DNA)

G.M. Huang; K. Wang; C.L. Kuo; B. Paeper; L. Hood A High-Throughput Plasmid DNA Preparation Method Analytical Biochemtry.1994;15;223(1):35-8

Robert R. Klein, Daryl T. Morishige, Patricia E. Klein, Jianmin Dong; John E. Mullet. High Throughput BAC DNA Isolation for Physical Map Construction of Sorghum. Plant Molecular Biology Reporter.1988;16(4):651-364

Deoxyribonucleic acid (DNA)

Kozekov ID; Turesky RJ; Alas GR; Harris CM; Harris TM; Rizzo CJ. Formation of Deoxyguanosine Cross-Links from Calf Thymus DNA Treated with Acrolein and 4-Hydroxy-2-nonenal. Chemical Research in Toxicology.2010;23(11):1701-1713

Applied Biosystems User Bulletin. Subject: Sequencing Large DNA Templates.

David C. Bruce; Mark O. Mundt; Kim K. McMurry; Linda J. Meincke; Donna L. Robinson; Norman A. Doggett; Larry L. Deaven. BAC Library End Sequencing in Support of Whole Genome Assemblies DOE Joint Genome Institute and Center for Human Genome Studies, Los Alamos National Laboratory

Dr. Domon, National Agricultural Research Center for Kyushu Okinawa Region, Japan, Extraction of Rush DNA, unpublished personal correspondence, 2004.

DNA Extraction Genetic Diversity as an Indicator of Ecosystem Condition and Sustainability for Regional Assessments of Stream Condition in the Eastern United States

Human Fibroblasts

Stephanie M Cohen, Terrence S Furey, Norman A Doggett, and David G Kaufman. Genome-wide sequence and functional analysis of early replicating DNA in normal human fibroblasts BMC Genomics.2006;7: 301

Norovirus, Adenovirus

Roberto A. Rodríguez, Lauren Thie, Christopher D. Gibbons, Mark D. Sobsey. Reducing the effects of environmental inhibition in quantitative real-time PCR detection of adenovirus and norovirus in recreational seawaters. Journal of Virological Methods;2012:181(S1):43-50

Concentration and purification of beef extract mock eluates from water samples for the detection of enteroviruses, hepatitis A virus, and Norwalk virus by reverse transcription-PCR. Applied and environmental microbiology.1995;61(2):531-7

Blood

John Krupey, Ph.D., Swapan Roy, Ph.D., & Bhavin Desai, et al, 100,000+ PCRs Possible from 10 ml Blood, poster Biotechniques Symposium, 2003
.

Human T-cell lymphotrophic virus I-induced adult T-cell leukemia (ATL)

J D Burton; R N Bamford; C Peters; A J Grant; G Kurys; C K Goldman; J Brennan; E Roessler; T A Waldmann. A lymphokine, provisionally designated interleukin T and produced by a human adult T-cell leukemia line, stimulates T-cell proliferation and the induction of lymphokine-activated killer cells Proceedings of the National Academy of Sciences of the United States of America.1994. 91(11): 4935-4939

Zebrafish genomic library of bacterial artificial chromosome (BAC) clones and BAC DNA

McDermott, Brian M., et al. Transgenic labeling of hair cells in the zebrafish acousticolateralis system. Gene Expression Patterns 10.2 (2010): 113-118.