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AlbuVoid™ LC-MS On-Bead For Serum Proteomics

AlbuVoid™ LC-MS On-Bead For Serum Proteomics


 
 


Functional Proteomics
Base Price: $585.00


Product Code: AVB-MS


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Description
 

Albumin Depletion Plus Low Abundance Serum Protein Enrichment With Optimized On-Bead Digestion for LC-MS Label and Label-free Analyses

  • Albumin and transferrin voids in flow-through >95%, with <30 minute bind/wash microfuge protocol
  • Low abundance enrichment equivalent or better than hexa-peptides or immuno-affinity
  • Consumable, cost-effective, no column regeneration or cross-contamination
  • Species agnostic; human, rat, mouse, goat, sheep, porcine and bovine sera have been tested
  • Trypsin digestion on the bead
  • Seamless workflows and unique proteolytic efficiencies
    • No in-gel digests, no solution digests, no C18 desalting, more consistent, reproducible results
    • Compatibility with quantitative label (i.e., iTRAQ) and label-free LC-MS methods

AlbuVoid™ LC-MS On-Bead is an albumin depletion reagent kit. It removes albumin from serum and plasma samples while concentrating low abundance proteins on the beads. Consequently, the low abundance serum proteins are enriched. It is ideal for applications involving LC-MS discovery and targeted proteomics.

The AlbuVoid beads are derived from a silica-based library of individual mixed-mode polymeric ligands. The library was designed to facilitate weak binding of proteins, allowing for progressive enrichment of the low abundance proteome, with specialized voiding properties empirically derived.

The total AlbuVoid™ LC-MS On-Bead proteins were compared for human and rat sera at two different digestion times, 4 hours and overnight (O/N). Note that many identified proteins overlap while certain populations of proteins were only observed in one or the other digest time. Download the detailed report

The AlbuVoid™ LC-MS On-Bead product and protocol is compatible with both label and label-free quantification of peptides/proteins. In the example below, iTRAQ labeled peptides from two representative proteins observed to be differentially quantified in comparing pooled sera from normal and cancer patients.



Click Here For The AlbuVoid™ LC-MS On-Bead For Serum Proteomics Product Sheet
References

Serum

Haiyan Zheng, Caifeng Zhao, Meiqian Qian, Swapan Roy, Absari Arpa, Amenah Soherwardy and Matthew Kuruc. AlbuVoid™ Coupled to On-Bead Digestion - Tackling the Challenges of Serum Proteomics. J Proteomics Bioinform 2015, 8:9.


Grubbs, J. K., et al. " Investigation of the efficacy of albumin removal procedures on porcine serum proteome profile1." (2015).


Discovery of Functional Serum Biomarkers Using AlbuVoid™ Enrichment and the ArrayBridge PEP Profiling Platform
.Personal communication, Xing Wang, Ph.D., ArrayBridge (St. Louis, MO), manuscripts in process.


Serum Profiling Making Mark on Predictive Medicine

Vicki Glaser.Genetic Engineering & Biotechnology News. 2011;31(7):1-55.


Kuruc Matt, Dr.Roy. Application Report -
AlbuVoid™ LC-MS On-Bead – Differential Expression of Cancer Sera Proteins Using Quantitative (iTRAQ) Proteomics


Plasma


Espes, Daniel, Joey Lau, and Per-Ola Carlsson. "Increased circulating levels of betatrophin in individuals with long-standing type 1 diabetes." Diabetologia(2013): 1-4.


Patent - Secretome Sample Preparation

Narain, Niven Rajin, and Paula Patricia Narain. "COMPOSITIONS AND METHODS FOR DIAGNOSIS AND TREATMENT OF PERVASIVE DEVELOPMENTAL DISORDER." U.S. Patent No. 20,150,023,949. 22 Jan. 2015.

Narain, Niven Rajin, Rangaprasad Sarangarajan, and Vivek K. Vishnudas. " INTERROGATORY CELL-BASED ASSAYS AND USES THEREOF." U.S. Patent No. 20,120,258,874. 11 Oct. 2012.


Cell Culture


Narain, Niven Rajin, Rangaprasad Sarangarajan, Vivek K. Vishnudas, and Michael Andrew Kiebish. "USE OF MARKERS IN THE IDENTIFICATION OF CARDIOTOXIC AGENTS AND IN THE DIAGNOSIS AND MONITORING OF CARDIOMYOPATHY AND CARDIOVASCULAR DISEASE." U.S. Patent 20,140,100,128, issued April 10, 2014.


On-Bead Digestion Protocols For LC-MS Proteomic Workflows


New on-bead digestion for LC-MS applications for proteomic studies


US HUPO 2014. Frontiers in Proteomics: Advancing Biology through Technology and Computation.


AlbuVoid™
abstract entitled "Improved proteomic enrichment and workflow strategies", poster board 089 presented at US HUPO 2014