AlbuTrial™ PLUS Kit

Trial-format kit for evaluating high abundance removal.
Species Agnostic
Suitable for LCMS, ELISA, Western Blot, Functional Analysis
Retains function and bio-activity

Product Code: AVPS

Trial kits includes 5 preps AlbuSorb™ PLUS and 5 preps AlbuVoid™ PLUS

AlbuSorb™ PLUS

Albumin + IgG Depletion Kit From Serum or Plasma

>400 µg total serum protein mass (> 85% Albumin, >85% IgG depleted) from 25 µl serum prep
Affinity-type equivalence for Albumin, IgGs, Complement, virtually no cross-reactivity with other proteins
Disposable, no column regeneration or cross-contamination
Combines unique bead technology, not based on Blue-dye affinity, with optimized Protein A
Mild conditions maintains structural integrity and simple transfer to secondary analysis
Suitable for immunoassay, Western blot, 1 & 2D Electrophoresis, enzyme assay, LC-MS
Suitable for most species including human, sheep, bovine, rabbit, mouse, rat

Click Here To View AlbuSorb™ PLUS Product Sheet

Click Here To View AlbuVoid™ PLUS Product Sheet

AlbuVoid™ PLUS
Albumin and IgG Depletion From Serum/Plasma for Proteomics

IgG removal >90% (70-80% of total Immunoglobulins removed)
Albumin removal >95% from 25 µl serum prep
Seamless and simple < 1 hour protocol
Low abundance enrichment equivalent to immuno-affinity
Disposable, cost-effective, no column regeneration or cross-contamination
Works for most species tested including human, sheep, rat, mouse, bovine
On-bead protocols improve workflow and efficiency, especially suited to targeted proteomics
Suitable for LC-MS, 1 and 2D Gels, ELISAs, Enzyme and other Functional Assays.

Product	Size	Quantity of Serum Processed	Product Code
AlbuTrial™ PLUS Kit	5 Preps of AlbuVoid™ PLUS and 5 preps of AlbuSorb™ PLUS	25 µl serum prep	AVPS

AlbuSorb™ References
Cerebrospinal Fluid
Gwenael Pottiez, Pawel Ciborowski. Proteomic Profiling of Cerebrospinal Fluid Expression Profiling In Neuroscience Neuromethods.2012;64:245-270
Synovial fluid
Happonen KE, Fürst CM, Saxne T et al. PRELP protein inhibits the formation of the complement membrane attack complex.Journal of Biological Chemistry.2012;287(11):8092-100
Serum
Holmberg R, Refai E, Höög A.Lowering apolipoprotein CIII delays onset of type 1 diabetes. Proceedings of the National Academy of Sciences.2011;108(26):10685-9.

Tang MX, Ogawa K, Asamoto M. Effects of Nobiletin on PhIP-Induced Prostate and Colon Carcinogenesis in F344 Rats Nutrition and Cancer.2011;63(2):227-33

Holmberg, Rebecka Apolipoprotein CIII and Ljungan virus in diabetes 2010. Doctoral Thesis

Lu Q, Zheng X, McIntosh T Development of different analysis platforms with LC-MS for pharmacokinetic studies of protein drugs. Analytical Chemistry.2009;81(21):8715-23
AlbuVoid™ References
Serum
Swapan Roy, Matthew Kuruc. The Functional Subproteomes of Serpin Protease Inhibitors are Now Open for LC-MS Biomarker Discovery. MOJ Proteomics Bioinform 2016, 3(6): 00106

Serum Profiling Making Mark on Predictive Medicine
Vicki Glaser.Genetic Engineering & Biotechnology News. 2011;31(7):1-55.
Patents
Narain, Niven Rajin, Rangaprasad Sarangarajan, and Vivek K. Vishnudas. "INTERROGATORY CELL-BASED ASSAYS AND USES THEREOF." U.S. Patent No. 20,120,258,874. 11 Oct. 2012.

Do BSG's products work for all species?

Yes, as our products are not derived from antibodies, they are species agnostic, and have applied to humans, mice, rats, guinea pigs, goats, cows, pigs, even frogs and fish.

Can you supply bulk material only (from the kit-based products) for large volume orders?

Yes, we can adjust all of our kit products, to supply only the beads. For large orders, whether kit or bulk material, we provide special discounted quotations. Please inquire.

Can the protocol be scaled to accommodate different amounts of either volume or mass?

Yes, we provide general guidelines for use, but the protocols can usually be adjusted to accommodate either larger or small starting samples, or larger or smaller starting concentrations. We recommend first contacting us for guidance in adjusting the protocol.

Can the products be adapted to high throughput formats and automation?

Although we do not have an off-the-shelf product for 96-well formats currently, we have ongoing collaborations that can provide such formats. Please inquire.

Are the products compatible with detergents?

This has not been fully investigated, but we do not recommend use of SDS. Low concentrations, 0.1 – 0.5% of non-ionic detergents have been used with some of our products, but to what extent they alter separation performance, is unknown. If non-ionic detergents are to be used, generally we recommend that they be of no greater than 0.1% before applying to the beads. So for tissue lysates for example at higher %, dilute the sample before application.

What is the stability and shelf life of the product?

The products have a shelf life typically 6 months to 1 year. We ship at room temperature, but recommend storage at 4°C upon receipt. Do not freeze.

Do the products contain any biologically derived material?

All of our products are derived chemically, not biologically, with one exception. The "PLUS" products contain recombinant Protein A.

How can I order?

We can take on-line cart orders, which accept North American credit cards, Visa & MasterCard. It does not however, accept other international credit cards. If you would like to order direct, contact us at [email protected]. In the US, distributors Fisher Scientific and VWR can be used to order all products, even those not listed on their sites, just provide them with our catalog #. A full list of international distributors is listed on How to Order | Biotech Support Group LLC.

MSDS:

MSDS-AlbuSorb™, Catalog Order # A185-
MSDS-AlbuVoid™, Catalog Order # AVK-10, AVK-50
MSDS-AlbuVoid™ Binding Buffer AVBB
MSDS-AlbuVoid™ Elution Buffer AVEB
MSDS-AlbuVoid™ Wash Buffer AVWB

Product Code	Name	Base Price	Qty
Product Code:AVPS-05	AlbuTrial PLUS Kit, 5 preps AlbuSorb PLUS & 5 Preps AlbuVoid PLUS	Base Price:$695.00

AlbuTrial™ PLUS Kit

Description

References

FAQ