Selection of AlbuVoid™ References:

Secretome from Cell Culture (BSA Removal)
Paulsen, Bruna, et al. " Reproducible differentiation of pure ovarian support cells from clinical-grade hiPSCs as a novel infertility treatment ." bioRxiv (2024): 2024-04. To gain insight into the potential mechanism of action of these cells during oocyte maturation beyond transcriptomics readouts, the investigators performed secretome proteomics to investigate proteins that overexpressed in OSC after 24 hours in vitro in comparison with OSCs prior to culture (0 hour) from cells derived, from both CG-hiPSC and RUO-hiPSC. For proteomic analysis, the article states, “The conditioned media … and then passed through albumin depletion columns (AlbuVoid) to eliminate HSA-derived albumin. ”.

Jenull, Sabrina, et al. "The histone chaperone HIR maintains chromatin states to control nitrogen assimilation and fungal virulence." Cell Reports 36.3 (2021): https://www.sciencedirect.com/science/article/pii/S2211124721008196#mmc1. The authors report a pivotal role for the HIR histone chaperone complex in modulating virulence of the human fungal pathogen Candida albicans . The article states for “Cell-free supernatants from 16 hours YNB-BSA (0.025% BSA) cultures grown at 30°C were used for Mass-Spec analysis. Collected supernatants were lyophilized and dissolved in 400 μl of water for AlbuVoid™ treatment for albumin depletion…Albumin-free enriched secretory proteome was eluted from beads”.

Serum/Plasma

Paulsen et al., Development of human induced pluripotent stem cell-derived ovarian support cells as a clinical-grade product for in vitro fertilization, Cell Stem Cell (2025), https://doi.org/10.1016/j.stem.2025.12.020. AlbuVoid™ enrichment, helped in characterizing key functionalities of human induced pluripotent stem cell (hiPSC) -derived cell lines.

Golden, Lesley R., et al. "APOE4 to APOE2 allelic switching in mice improves Alzheimer’s disease-related metabolic signatures, neuropathology and cognition ." Nature Neuroscience (2025): 1-15. ApoE enrichment with AlbuVoid™, helped in identifying the closely related ApoE4/2 variants by targeted plasma proteomic analysis.

Sae-Lee, Wisath, et al. " The protein organization of a red blood cell ." Cell Reports 40.3 (2022): 111103. For plasma preparation, “Plasma was centrifuged at 5,000 x g for 15 mins at 4°C, and only the top part of supernatant was collected. The supernatant was treated with AlbuVoid (Biotech Support Group) to reduce the presence of serum albumin .”

Vialaret, Jerome & Kadi, Sarah & Tiers, Laurent & O Flynn, Robin & Lehmann, Sylvain & Hirtz, Christophe. (2018). Albumin depletion of human serum to improve quantitative clinical proteomics. Current Topics in Peptide & Protein Research 19. 53-62. http://www.researchtrends.net/tia/abstract.asp?in=0&vn=19&tid=26&aid=6192&pub=2018&type=3

In this work, the investigators focused on depleting albumin from human serum samples using an albumin depletion and low abundance protein enrichment kit – AlbuVoid™, which enabled the detection of several low-abundance proteins. By employing an optimized protocol, enriched proteins known as biomarkers for various diseases were identified. The authors concluded that the AlbuVoid™ depletion method proved to be faster and more cost-effective than antibody based methods , and could be helpful for biomarker enrichment and detection in medical research.

Jing, Lun, et al. " PROTEOMIC ANALYSIS IDENTIFIED LBP AND CD14 AS KEY PROTEINS IN BLOOD/BIPHASIC CALCIUM PHOSPHATE MICROPARTICLE INTERACTIONS ." Acta Biomaterialia (2021). Here, in a LC-MS/MS proteomic study, the article describes use of HemoVoid™ and AlbuVoid™ prior to LC-MS analysis, “…After albumin depletion , analysis of the significant deregulated proteins showed that 27 signaling pathways significantly changed in blood cells… ”

Poillet-Perez, Laura, et al. "Autophagy maintains tumour growth through circulating arginine ."Nature (2018): 1.

One in vivo model to study autophagy is whole-body deletion of the essential autophagy gene Atg7 in adult mice which causes a systemic metabolic defect that manifests as starvation intolerance. In order to measure the systemic proteomic response of such deletion in this study, AlbuVoid™ was chosen as one method to remove albumin from mice and enrich the low abundance proteomes from serum.

Zheng H, Zhao C, Qian M, Roy S, Arpa A, et al. (2015) AlbuVoid™ Coupled to On-Bead Digestion – Tackling the Challenges of Serum Proteomics. J Proteomics Bioinform 8: 225-230. The AlbuVoid™ bead enrichment is combined with a direct and seamless integration with Trypsin digestion, a method conventionally referred to as on-bead digestion. The digest time was evaluated as a parameter to identify whether different sub-populations of peptides and proteins can be observed by LC-MS analyses. Using 2 different digestion times – 4 hours, and overnight, each with a singular 3 hour gradient LC-MS run, between 400-500 total proteins were observed for both human and rat sera, with overlapping and distinct sub-populations observable at each digest time. These results support that the described methods gain workflow efficiencies over other high abundance depletion and in-solution digestion workflows .

Swapan Roy, Matthew Kuruc. The Functional Subproteomes of Serpin Protease Inhibitors are Now Open for LC-MS Biomarker Discovery . MOJ Proteomics Bioinform 2016, 3(6). Using bead-based separation provided by the NuGel™ family of proteomic enrichment products - notably AlbuVoid™ & AlbuSorb™, the authors demonstrate their utility to satisfy investigations of serum SERPINs. The authors also suggest their use to develop functional profiles of the SERPIN proteoforms, and how those can establish relationships to disease phenotypes, gene variant, and dysregulated mechanisms.

David L. Wang, Chuanguang Xiao, Guofeng Fu, Xing Wang and Liang Li. “ Identification of potential serum biomarkers for breast cancer using a functional proteomics technology ”. Biomarker Research (2017) 5:11.

The article states “The most dramatic difference for enzyme activity detection in using the AlbuVoid™ for serum protein enrichment was demonstrated in the case of protease activity analysis . Compared with the direct serum proteinase measurement, both the levels and species of proteases were increased significantly in the enriched serum sample. …, and it is necessary to use AlbuVoid™ to enrich these low level proteases to bring them to a high enough level to be detected .”

For a full list of Albumin Removal references, visit: https://www.biotechsupportgroup.com/References-s/138.htm#albumin-depletion

Albumin Depletion Kits from Serum or Plasma

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AlbuVoid™ - Albumin Depletion and Low Abundance Protein Enrichment Kit from Serum, Plasma, Tissues And Culture Media

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