NewsRelease

Research Article Cites NuGel™PBA for Proteome Analysis of Saliva

MONMOUTHJUNCTION, NJ,December12, 2017 -- Biotech Support Group reports on a recent researcharticle describing the simplicity and efficiency of their NuGel™PBAglycoproteinenrichment sample preparation technology for the proteomic analysisof saliva.

Thecitation is:

Gutiérrez,Ana, et al. "Influence of different sample preparationstrategies on the proteomic identification of stress biomarkers inporcine saliva." BMCVeterinary Research13.1 (2017): 375. https://bmcvetres.biomedcentral.com/articles/10.1186/s12917-017-1296-9

Theauthors describe theinfluence of two different sample pre-treatments on the detection ofstress biomarkers in saliva. Both the enrichment of glycoproteins byimmobilized boronic acid (PBA) and sub-proteome enrichment by dynamicrange compression were considered in this study. For this purpose,saliva samples collected before and after the application of an acutestress model in pigs were evaluated. Two-dimensional gelelectrophoresis (2-DE) followed by MALDI-TOF/TOF mass spectrometry(MS) was used as the proteomic reporting technique.The article states “1mgof total protein from each salivary pool was lyophilized andsubjected to PBA treatment. A commercial glycoprotein enrichment kitbased on PBA matrix was used (NuGel™ Glycoprotein enrichmentPBA kit, Biotech Support Group) according to the manufacturer’sinstructions.” In brief, “the freeze-dried PBA saliva wasdissolved in 250μL of binding buffer, added to 50mg of PBA matrixand incubated for 10min. The unbound fraction was discarded…thematrix was washed three times with 350μL of wash buffer, and theglycoprotein-enriched fraction was obtained by incubation of thematrix with 300μL of sorbitol elution buffer…”.The authors concluded that theuse of different procedures allowed the detection of differentpotential stress biomarkers. From a practical point of view, the useof saliva without treatment as well as the enrichment ofglycoproteins with NuGel™ PBA were preferred.

“Thisreference shows the versatility of NuGel™PBAto efficiently enrich glycoproteins from a readily accessiblebiofluid, with a selectivity profile suitable for proteome analysisand biomarker discovery using conventional proteomic analyses.”states Swapan Roy, Ph.D., President and Founder of Biotech SupportGroup. 

Formore information on glycoprotein enrichment products, visit:

ForNuGel™Phenyl Boronic Acid:

http://www.biotechsupportgroup.com/NuGel-Phenyl-Boronic-Acid-p/npba.htm

ForNuGel™GlycoproteinPBA Enrichment Kit:

http://www.biotechsupportgroup.com/NuGel-Glycoprotein-Enrichment-PBA-Kit-p/ngpba.htm

AboutBiotech Support Group LLC

Convergingwith cultural and technological disruptions forthcoming inhealthcare, Biotech Support Group develops methods for cost effectiveand efficient sample prep essential for these expanding markets.Following a tiered business strategy, the company continues itsgrowth in the consumable research products area supporting therapidly expanding installation of LC-MS instrument and computationalinfrastructure. For this market, key products include: AlbuVoid™and AlbuSorb™ for albumin depletion, Cleanascite™ forlipid adsorption, HemogloBind™ and HemoVoid™ forhemoglobin removal, and NuGel™ for functional proteomics. Fromthese innovations, the company has acquired knowledgebase andbiomarker intellectual property assets that support discoveries ofprotein markers from blood, with special emphasis on early detectionand personalized medical decisions for cancer patients. For moreinformation, go tohttp://www.biotechsupportgroup.com.

Contact:MatthewKuruc 732-274-2866 [email protected]