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  KinaSorb™ Kinase (& ATP binding proteins) Enrichment Reagent



 
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Price: $365.00

Product Code: KE785


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Description Applications Specifications
 

KinaSorb™ Kinase (& ATP binding proteins) Enrichment Reagent

  • Ratio of Total Kinase Activity* relative to total protein content increases 300% or more
  • ATP binding protein recoverable, ~200 ug
  • Up to 4X concentration of kinase volume, relative to starting volume
  • 60 minute, scaleable protocol compatible with functional assays, electrophoresis and Mass Spec
  • Phosphatase (neutral & alkaline) activity not detectable
  • Improves protein normalization when comparing heterogeneous tissues

KinaSorb™ is a new reagent kit used for the enrichment and isolation of kinases. In a patent pending process, ATP – the common substrate for kinases, is reversibly immobilized to metallic oxide particles, producing a single-use highly efficient enrichment method for kinase & other ATP binding proteins. The standard prep protocol starts with 100 µl of clarified cellular extracts, or approximately 2 mg total cellular extract protein, but the process can be scaled up or down to accommodate different sample volumes and protein concentrations. The methods are reasonably specific to ATP; little Phosphodiesterase activity (an enzyme which binds to a structurally similar adenosine containing substrate - CAMP), was measured in the eluant after KinaSorb™ treatment. The kit includes all necessary reagents for immediate use.



Click Here To View Kinasorb™ Product Sheet
References

Xing Wang, Michael Davies, Swapan Roy and Matthew Kuruc. Bead Based Proteome Enrichment Enhances Features of the Protein Elution Plate (PEP) for Functional Proteomic Profiling. Proteomes 2015, 3(4), 454-466.

doi: 10.3390/proteomes3040454