Genomic Sample Prep
ProCipitate™
Superior Substitute to Phenol/Chloroform for Hemoglobin & Protein Removal, Isolation of DNA/RNA
- Removes protein contaminants & leaves DNA & RNA soluble and unreacted
- Ideal for applications when the alternative kits don’t fit, or are not optimal
- Adaptable to any sample size and can be automated
- Pathogen and infectious disease testing
| Sample Type | ProCipitate™ Typical Usage |
|---|---|
| 1 mm Plant Leaf | 50 µl |
| 2.0 ml culture BAC Preps | 80 µl |
| 5µm paraffin-embedded tissue | 200 µl |
| Dried Blood Card or ~ 40 µl Whole Blood | 400 µl |
| 200µl lysed cell pellet | 200 µl |
PCR from whole blood using ProCipitate™
Lane 1: 100-1000 base pair Ladder
Lane 2: Negative Control
Lanes 3-10:
PCR amplicons from 1 ng template DNA purified from whole blood, randomly selected from 96 wells. Amplicons are 280 base pairs from Human HLA-DRBeta primers at 32 cycles.
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Key References
*J M Kelley et al. High Throughput Direct End Sequencing of BAC Clones. Nucleic Acids Research.1999.15;27(6):1539-1546
U.S. Patent Number 5,538,870, Method for Preparing Nucleic Acids For Analysis And Kits Useful Therefore. This patent shows the beneficial effects of ProCipitate™ in protocols which neutralize SDS with non-ionic detergents, are PCR compatible, and require no alcohol precipitation.
ProCipitate™ appears in several articles and books on Food Safety
Foodborne Disease Handbook, Second Edition,: Volume 2: Viruses: Parasites By Y. H. Hui, Sayed A. Sattar, Wai-Kit Nip “Viruses in the PEG eluants were precipitated…by an equal volume of ProCipitate™.”
D’Souza, D. H. “Update on foodborne viruses: types, concentration and sampling methods.” Advances in Microbial Food Safety 2 (2014): 102.
Health-related Water Microbiology, Volume 27, Issues 3-4, Pergamon, 1993 “ProCipitate™ was an effective method to purify the sample and dramatically improve virus detectability by RT-PCR.”
Cleanascite™
Lipid Adsorption & Clarification
- Effectively replaces chlorinated/fluorinated hydrocarbons (eg. freon)
- Extensively cited in journal articles
- microRNA from egg yolk
- genome of Mycobacterium tuberculosis H37Rv
- RNA from sputum
- Intestinal microbial DNA
Egg Yolk Clarification
Insert: PAGE showing
Left: Markers
Right: IgY and other major protein fractions recovered
Viraffinity™ & ViraPrep™ Kits
Virus Enrichment & Purification
- Purifies whole infectious non-enveloped virus, isolates antigenic virions
- Enriches for viral proteins and nucleic acids
Lanes M: Markers
Lane 1: PEG/Phenol-Chloroform
Lane 2: ViraPrep™ Lambda methods
Lane 3: Eco R1 digest of ViraPrep™ Lambda DNA
Note: Insert band apprx. 1 kb
Viraffinity™
Prep Size: Application dependent
View DetailsViraPrep™ Lambda
Prep Size: 5, 150mm platelysates
View DetailsViraPrep™ Mammal
Prep Size: 40 ml
View DetailsOur BSG Advantage
Consumable
Cost-effective, not derived from biologicals
- No specialized instruments or HPLC
- Economical surface chemistries, not derived from biologicals
- No regeneration, so no prep to prep variability
- Simple, fast microfuge bind/wash/elute protocols
On-Bead Digestion
Efficient workflows, quality LC-MS/MS data
- Simple, reproducible workflows
- Equivalent or better than in-solution digestion
- Seamless to LC-MS, no desalting or C18 separations
- Unique proteolytic efficiencies
Enrichment/Depletion
Diverse strategies, species agnostic
- Products support strategies for both enrichment of low abundance proteomes, or depletion of high abundance proteins
- Species agnostic not derived from biologicals
Functional Integrity
Maintained throughout all separations
- Mild buffer conditions maintains native structure with retained enzymatic, functional & bio-activities
- Supports enzyme biomarker assays
- Functional & Chemical Proteomics
- Structural & activity-probe Proteomics
- Top-down & ArrayBridge PEP Proteomics