Genomic Sample Prep


ProCipitate™

Superior Substitute to Phenol/Chloroform for Hemoglobin & Protein Removal, Isolation of DNA/RNA

  • Removes protein contaminants & leaves DNA & RNA soluble and unreacted
  • Ideal for applications when the alternative kits don’t fit, or are not optimal
  • Adaptable to any sample size and can be automated
  • Pathogen and infectious disease testing

Sample Type ProCipitate ™ Typical Usage
1 mm Plant Leaf 50 µl
2.0 ml culture BAC Preps 80 µl
5µm paraffin-embedded tissue 200 µl
Dried Blood Card or ~ 40 µl Whole Blood 400 µl
200µl lysed cell pellet 200 µl

PCR from whole blood using ProCipitate™

PCR from whole blood using ProPrep Genomic kit

Lane 1: 100-1000 base pair Ladder

Lane 2: Negative Control

Lanes 3-10:
PCR amplicons from 1 ng template DNA purified from whole blood, randomly selected from 96 wells. Amplicons are 280 base pairs from Human HLA-DRBeta primers at 32 cycles.


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Key References

*J M Kelley et al. High Throughput Direct End Sequencing of BAC Clones. Nucleic Acids Research.1999.15;27(6):1539-1546

U.S. Patent Number 5,538,870, Method for Preparing Nucleic Acids For Analysis And Kits Useful Therefore. This patent shows the beneficial effects of ProCipitate™ in protocols which neutralize SDS with non-ionic detergents, are PCR compatible, and require no alcohol precipitation.

ProCipitate™ appears in several articles and books on Food Safety

Foodborne Disease Handbook, Second Edition,: Volume 2: Viruses: Parasites By Y. H. Hui, Sayed A. Sattar, Wai-Kit Nip “Viruses in the PEG eluants were precipitated…by an equal volume of ProCipitate™.”

D’Souza, D. H. “Update on foodborne viruses: types, concentration and sampling methods.” Advances in Microbial Food Safety 2 (2014): 102.

Health-related Water Microbiology, Volume 27, Issues 3-4, Pergamon, 1993 “ ProCipitate™ was an effective method to purify the sample and dramatically improve virus detectability by RT-PCR.”

Cleanascite™

Lipid Adsorption & Clarification

  • Effectively replaces chlorinated/fluorinated hydrocarbons (eg. freon)
  • Extensively cited in journal articles
    - microRNA from egg yolk
    - genome of Mycobacterium tuberculosis H37Rv
    - RNA from sputum
    - Intestinal microbial DNA
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Egg Yolk Clarification


Egg Yolk Clarification
Insert: PAGE showing
Left: Markers
Right: IgY and other major protein fractions recovered

Viraffinity™ & ViraPrep™ Kits

Virus Enrichment & Purification

  • Purifies whole infectious non-enveloped virus, isolates antigenic virions
  • Enriches for viral proteins and nucleic acids
M12M3

Lanes M: Markers
Lane 1: PEG/Phenol-Chloroform
Lane 2: ViraPrep™ Lambda methods
Lane 3: Eco R1 digest of ViraPrep™ Lambda DNA
Note: Insert band apprx. 1 kb

Viraffinity™

Prep Size: Application dependent

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ViraPrep™ Lambda

Prep Size: 5, 150mm platelysates

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ViraPrep™ Mammal

Prep Size: 40 ml

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Our BSG Advantage


Consumable

Cost-effective, not derived from biologicals

Cost-effective, not derived from biologicals

  • No specialized instruments or HPLC
  • Economical surface chemistries, not derived from biologicals
  • No regeneration, so no prep to prep variability
  • Simple, fast microfuge bind/wash/elute protocols

On-Bead Digestion

Efficient workflows, quality LC-MS/MS data

Efficient workflows, quality LC-MS/MS data

  • Simple, reproducible workflows
  • Equivalent or better than in-solution digestion
  • Seamless to LC-MS, no desalting or C18 separations
  • Unique proteolytic efficiencies

Enrichment/Depletion

Diverse strategies, species agnostic

Diverse strategies, species agnostic

  • Products support strategies for both enrichment of low abundance proteomes, or depletion of high abundance proteins
  • Species agnostic not derived from biologicals

Functional Integrity

Maintained throughout all separations

Maintained throughout all separation

  • Mild buffer conditions maintains native structure with retained enzymatic, functional & bio-activities
  • Supports enzyme biomarker assays
  • Functional & Chemical Proteomics
  • Structural & activity-probe Proteomics
  • Top-down & ArrayBridge PEP Proteomics