NewsRelease
TheUse of HemogloBind™ in Proteomic Analysis of Programmed Death ofRed Blood Cells
MONMOUTHJUNCTION, NJ, December13, 2023 -- Biotech Support Group reports on a journal articledescribing the simplicity and efficiency of HemogloBind™, to removeHemoglobin interference prior to LC-MS proteomic analysis of redblood cells.
Thecitation is:
Chen,Yaozhen, et al. "NoncanonicalNLRP3 Inflammasome Activation Elicits the Programmed Death of RedBlood Cells." Availableat SSRN 4626203.
Thecanonical complement-mediated lysis of red blood cells (RBCs) leadsto serious pathogenesis. To seek more efficient therapy, the authorsinvestigated the intracellular events in RBCs following complementactivation. They demonstrate that complement-induced hemolysis wascaspase-8-dependent. So to investigate the downstream signaling ofcaspase-8 in mature RBCs, label free proteomic analysis wasconducted. The article states “RBC lysates (control andcomplement-treated groups) … Hemoglobin was removed by adding 250µl of HemogloBind suspension (Biotech Support Group) to the celllysate. …Subsequently, samples without hemoglobin were analyzedusing chromatography-tandem mass spectrometry (LC-MS/MS)”. Thisanalysis identified proteins with both upregulated and downregulatedexpressions in response to complement activation and revealed asignificant involvement of biological processes and pathwaysassociated with cytoskeleton organization and actin filament assemblydynamics. Overall, the findings unveil a programmed death cascade inmature RBCs, which may have implications for the treatment ofhemolytic disorders.
“Iam pleased to see that the use of HemogloBind™ was necessary toremove such a highly abundant protein – Hemoglobin, so thatproteomic analysis on red cell hemolysates became possible. Theobservations here demonstrate how HemogloBind™ can be used touncover proteome differences between two red cell samples types, thecontrol and complement-treated groups.”states Swapan Roy, Ph.D., President and Founder of BiotechSupport Group.
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Keywords:Hemoglobindepletion, HemogloBind™, redcell proteomics, NLRP3, inflammasome, caspase-8, spectrin, programmedcell death, red blood cells, complement hemolysis
AboutBiotech Support Group LLC
Convergingwith cultural and technological disruptions forthcoming inhealthcare, Biotech Support Group develops methods for cost effectiveand efficient sample prep essential for expanding proteomic analysis.Following a tiered business strategy, the company continues itsgrowth in the consumable research products area. For this market, keyproducts include: AlbuVoid™ and AlbuSorb™ PLUS foralbumin & IgG depletion, Cleanascite™for lipid adsorption, HemogloBind™ and HemoVoid™ for hemoglobinremoval, and NRicher™ for low abundance and family specificproteome enrichment. For more information, go tohttp://www.biotechsupportgroup.com
Forbusiness development contact: MatthewKuruc
732-274-2866[email protected]