Hemoglobin Removal Kits
HemoVoid™
Hemoglobin Depletion For Erythrocyte Proteomics
- Hemoglobin voids in flow-through, applicable to red cells, heavily hemolyzed serum, whole blood and dried blood spot (DBS) card
- Low abundance protein and enzyme enrichment
- Consumable, cost-effective
- Mild elution maintains native structure with retained enzymatic, functional and bioactivities
- Compatible with LC-MS, activity-probe profiling and virtually all proteomic analyses
2DE Comparison.
Red circles indicate the Hemoglobin subunits region. The HemoVoid™ eluate (bottom) has been severely depleted of Hemoglobin. The remainder of the red cell proteins are substantially enriched (visualized) and are better resolved in the HemoVoid™ eluate. Many more proteins are detectable after HemoVoid™ treatment with extensive proteome coverage across both dimensions.
HemogloBind™
and
NuGel™ HemogloBind™
Removes Hemoglobin Interference
- Highly specific for hemoglobin binding
- depletion from hemolyzed serum, dura, BALF, and whole blood
- Functional integrity maintained with simple transfer to post-treatment interrogations
- supports biomarker tests
HemoVoid™ LC-MS On-Bead
Hemoglobin depletion plus low abundance protein enrichment with optimized on-bead digestion for LC-MS erythrocyte & whole blood proteomics
- Seamless workflows
- Unique proteolytic efficiencies
- Label, label free & phospho- compatible
HemoVoid™ Blood Card Kit
The HemoVoid™ Blood Card kit substantially reduces hemoglobin interference from dried blood spot protein analytes
View Details
HemogloBind™ Nucleic Acid
Suspension Reagent to remove Hemoglobin interference for PCR
- Suitable for infectious disease research or rigorous analysis for whole blood or heavily hemolyzed samples
- Scaleable suspension reagent
- Robust and simple, no phenol/chloroform or ammonium sulfate precipitation
- High Yield – free and soluble DNA or RNA is highly recoverable
- > 95% Hemoglobin and Protein removal from whole blood lysates & dried blood cards
- Eliminates inhibitory effects of heme for PCR applications
- Compatible with silica-type final isolation preps
HemogloBind™ Blood Card Kit
Hemoglobin Depletion and Protein Enrichment From Dried Blood Spots
- Dried blood spots are useful for low volume analyses, and simple collection and transport
- Protocols suitable for inexpensive whole blood card systems, no need for cell separation
- Hemoglobin binding >90%, with 30-45 minute spin-filter format
- Protocols based on ≤10 µl whole blood applied, but suspension format is flexible to most volumes
- Blood proteins and enzymes are enriched for biomarker and proteomic investigations.
- Removes hemoglobin from diverse species including human, sheep, bovine, goat, rat, mouse, etc.
- High throughput easicly scalable.
Flow chart of HemogloBind™ Blood Card Application:
Absorbance at 410nm shows presence of hemoglobin
HemoVoid™ Hemoglobin Variant Enrichment from Blood
Purification & Enrichment Of Hemoglobin From Blood For Hemoglobin Variant Research
View DetailsOur BSG Advantage
Consumable
Cost-effective, not derived from biologicals
- No specialized instruments or HPLC
- Economical surface chemistries, not derived from biologicals
- No regeneration, so no prep to prep variability
- Simple, fast microfuge bind/wash/elute protocols
On-Bead Digestion
Efficient workflows, quality LC-MS/MS data
- Simple, reproducible workflows
- Equivalent or better than in-solution digestion
- Seamless to LC-MS, no desalting or C18 separations
- Unique proteolytic efficiencies
Enrichment/Depletion
Diverse strategies, species agnostic
- Products support strategies for both enrichment of low abundance proteomes, or depletion of high abundance proteins
- Species agnostic not derived from biologicals
Functional Integrity
Maintained throughout all separations
- Mild buffer conditions maintains native structure with retained enzymatic, functional & bio-activities
- Supports enzyme biomarker assays
- Functional & Chemical Proteomics
- Structural & activity-probe Proteomics
- Top-down & ArrayBridge PEP Proteomics