NewsRelease
Cleanascite™Sample Prep Improves Nanopore Detection of microRNA from Saliva
MONMOUTHJUNCTION, NJ, August26, 2024 -- Biotech Support Group reports on a journal articledescribing the simplicity and efficiency of using Cleanascite™ toeliminate interferences from lipids and lipoproteins thatnonspecifically interact with a nanopore channel.
Thecitation is:
Arora,Pearl, et al. "Nanopore”baseddetection of periodontitis biomarker miR31 in salivasamples." Electrophoresis (2024).
Aberrationsin the microRNAs (miRNA) levels have been the cause behind variousdiseases, including periodontitis. Therefore, sensitive, specific,and accurate detection of disease-associated miRNAs is vital to earlydiagnosis and can facilitate inhibitor screening and drug design. Inthis study, the researchers developed a label-free, real-time sensingmethod for the detection of miR31, which has been frequently linkedto periodontitis, using an engineered protein nanopore and in thepresence of a complementary ssDNA as a molecular probe. When humansaliva was tested however, extended pore blockage with large noiselevels was noted due to the presence of interfering lipid orlipoprotein complexes. To circumvent this, the article states “wefound that that the nonspecific pore blockage by the sample matrixcould be significantly diminished by simply treating human salivasamples with Cleanascite. …By taking advantage of Cleanascitetreatment, the saliva samples produced current modulations withsimilar signatures to that of the miR31-p5 duplex in the control(i.e., in water solutions).” The authors conclude that their methodis rapid and highly sensitive with nanomolar concentration of miR31that could be determined in minutes. In addition, artificial salivaand human saliva samples were successfully analyzed. The developednanopore sensing platform could be used to detect other miRNAs andoffers a potential application for the clinical diagnosis of diseasebiomarkers.
“Iam pleased to see that this is the second reported use ofCleanascite™ for removing interferences from lipids andlipoproteins in nanopore analysis. Its simplicity in use and lipidremoval efficiency should extend to eventual clinical analysis ofdisease biomarkers from saliva.”statesSwapan Roy, Ph.D., President and Founder of Biotech Support Group.
Formore information visit: Cleanascite™ Lipid Removal Reagent andClarification, at
http://www.biotechsupportgroup.com/Cleanascite-Lipid-Removal-Reagent-p/x2555.htm
Keywords:Cleanascite™,lipid interference, Saliva biomarkers, microRNA analysis, nanoporeanalysis
AboutBiotech Support Group LLC
Convergingwith cultural and technological disruptions forthcoming inhealthcare, Biotech Support Group develops methods for cost effectiveand efficient sample prep essential for expanding proteomic analysis.Following a tiered business strategy, the company continues itsgrowth in the consumable research products area. For this market, keyproducts include: AlbuVoid™ and AlbuSorb™ PLUS foralbumin & IgG depletion, Cleanascite™for lipid adsorption, HemogloBind™ and HemoVoid™ for hemoglobinremoval, and NRicher™ for low abundance and family specificproteome enrichment. For more information, go tohttp://www.biotechsupportgroup.com
Forbusiness development contact: MatthewKuruc
732-274-2866[email protected]