News Release

Nature Article Cites AlbuSorb™ for Analysis of Intestinal Organoids in Crohn’s Disease

MONMOUTH JUNCTION, NJ, October 11, 2022 -- Biotech Support Group reports on a Nature article showing the use of AlbuSorb™ for immunoblotting of intestinal organoid supernatants.

Matsuzawa-Ishimoto, Yu, et al. "The γδ IEL effector API5 masks genetic susceptibility to Paneth cell death." Nature (2022): 1-8.

Loss of Paneth cells and their antimicrobial granules compromises the intestinal epithelial barrier and is associated with Crohn’s disease. Non-classical lymphoid cells, broadly referred to as intraepithelial lymphocytes (IELs), intercalate the intestinal epithelium. The identification of mediators that coordinate crosstalk between specific IEL and epithelial subsets could provide insight into intestinal barrier mechanisms in health and disease. Here we show that the subset of IELs that express γ and δ T cell receptor subunits (γδ IELs) promotes the viability of Paneth cells deficient in the Crohn’s disease susceptibility gene ATG16L1. For immunoblotting of intestinal organoid supernatants, the article states “…albumin was depleted with AlbuSorb… according to the manufacturer’s instructions”. The authors identified apoptosis inhibitor 5 (API5) as a protective γδ IEL effector that masks genetic susceptibility to Paneth cell death.

“This reference shows that AlbuSorb™ is very selective for albumin depletion, and consequently improved immunoblot analysis. Also, as our bead technology does not rely on immuno-affinity, it works for all species; mice in this example.”, states Swapan Roy, Ph.D., President and Founder of Biotech Support Group.

For more information visit: AlbuSorb™ at
http://www.biotechsupportgroup.com/AlbuSorb-Albumin-Depletion-Kit-p/a185.htm

For more information on our complete line of Albumin & IgG Removal, visit:

https://www.biotechsupportgroup.com/Albumin-Removal-s/307.htm

About Biotech Support Group LLC

Converging with cultural and technological disruptions forthcoming in healthcare, Biotech Support Group develops methods for cost effective and efficient sample prep essential for expanding proteomic analysis. Following a tiered business strategy, the company continues its growth in the consumable research products area. For this market, key products include: AlbuVoid™ and AlbuSorb™ PLUS for albumin & IgG depletion, Cleanascite™ for lipid adsorption, and HemogloBind™ and HemoVoid™ for hemoglobin removal. From these innovations, the company has acquired knowledgebase and biomarker intellectual property assets that support discoveries of protein markers from blood, with special emphasis on early detection and personalized medical decisions for cancer patients. For more information, go to http://www.biotechsupportgroup.com.

For business development contact: Matthew Kuruc
732-274-2866 mkuruc@biotechsupportgroup.com

Keywords: Paneth cells, Crohn’s disease, AlbuSorb™, Albumin Removal, organoid supernatants