NewsRelease

NatureArticle Cites AlbuSorb™ for Analysis of Intestinal Organoids inCrohn’s Disease

MONMOUTHJUNCTION, NJ,October11, 2022 -- Biotech Support Group reports on a Nature article showingthe use of AlbuSorb™for immunoblotting of intestinal organoid supernatants.

Matsuzawa-Ishimoto,Yu, et al. "Theγδ IEL effector API5 masks geneticsusceptibilityto Paneth cell death." Nature (2022):1-8.

Lossof Paneth cells and their antimicrobial granules compromises theintestinal epithelial barrier and is associated with Crohn’sdisease. Non-classical lymphoid cells, broadly referred to asintraepithelial lymphocytes (IELs), intercalate the intestinalepithelium. The identification of mediators that coordinate crosstalkbetween specific IEL and epithelial subsets could provide insightinto intestinal barrier mechanisms in health and disease. Here weshow that the subset of IELs that express γ and δ T cell receptorsubunits (γδ IELs) promotes the viability of Paneth cells deficientin the Crohn’s disease susceptibility gene ATG16L1.For immunoblotting of intestinal organoid supernatants, the articlestates “…albumin was depleted with AlbuSorb… according to themanufacturer’s instructions”. The authors identified apoptosisinhibitor 5 (API5) as a protective γδ IEL effector that masksgenetic susceptibility to Paneth cell death.

“Thisreference shows that AlbuSorb™ is very selective for albumindepletion, and consequently improved immunoblotanalysis. Also, as our bead technology does not rely onimmuno-affinity, it works for all species; mice in this example.”,states Swapan Roy, Ph.D., President and Founder of Biotech SupportGroup.

Formore information visit: AlbuSorb™at
http://www.biotechsupportgroup.com/AlbuSorb-Albumin-Depletion-Kit-p/a185.htm

Formore information on our complete line of Albumin & IgG Removal,visit:

https://www.biotechsupportgroup.com/Albumin-Removal-s/307.htm

AboutBiotech Support Group LLC

Convergingwith cultural and technological disruptions forthcoming inhealthcare, Biotech Support Group develops methods for cost effectiveand efficient sample prep essential for expanding proteomic analysis.Following a tiered business strategy, the company continues itsgrowth in the consumable research products area. For this market, keyproducts include: AlbuVoid™ and AlbuSorb™ PLUS foralbumin & IgG depletion, Cleanascite™for lipid adsorption, and HemogloBind™ and HemoVoid™ forhemoglobin removal. From these innovations, the company has acquiredknowledgebase and biomarker intellectual property assets that supportdiscoveries of protein markers from blood, with special emphasis onearly detection and personalized medical decisions for cancerpatients. For more information, go tohttp://www.biotechsupportgroup.com.

Forbusiness development contact: Matthew Kuruc
732-274-2866[email protected]

Keywords:Paneth cells, Crohn’s disease, AlbuSorb™,Albumin Removal, organoid supernatants