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NatureArticle Cites NuGel™-HemogloBind™ in Study of ReprogrammingTumour-associated Macrophages

MONMOUTHJUNCTION, NJ, July17, 2023 -- Biotech Support Group reports on a journal articledescribing the simplicity and efficiency of NuGel™ HemogloBind™,to remove Hemoglobin interference, in a LC-MS amino acid analysis ofmouse tumor interstitial fluid.

Thecitation is:

Zhang,X., Li, S., Malik, I. etal. Reprogrammingtumour-associated macrophages to outcompete cancercells. Nature(2023). https://doi.org/10.1038/s41586-023-06256-5

Tumoursare metabolically active and are populated by stroma cells, but howenvironmental factors affect cancer cell competition remains largelyunknown. The study evaluated how tumour-associated macrophages (TAMs)can be dietarily or genetically reprogrammed to outcompeteMYC-overexpressing cancer cells. In a mouse model of breast cancer,MYC overexpression resulted in an mTORC1-dependent ‘winner’cancer cell state. To measure the amino acid content by LC-MS fromtumor interstitial fluid, the article states “Samples were brought through a NuGel-HemogloBind(Biotech Support Group) prep prior to extraction to remedy the levelsof haemolysis present. Twenty microliters of sample was added to amicrofuge tube and mixed with 200 µl of HemogloBind BindingBuffer…The total volume (220 µl) was then transferred to aSpin-filter tube that was pre-conditioned with 50 mgNuGel-HemogloBind beads. The spin-filter tubes were then vortexedagain at room temperature for 10 min before centrifuging at 8,000gfor 4 min.” The article concludes noncanonical engulfment-medieated RagGTPase-independent mTORC1 signalling in TAMs controls competitionbetween TAMs and cancer cells, which defines a novel innate immunetumour suppression pathway that could be targeted for cancer therapy.


“Hereis another example of the versatility of NuGel™ HemogloBind™, asit is used here to clear Hemoglobin interference from tumorinterstitial fluid in LC-MS amino acid analysis. This alsodemonstrates another key feature of our products. As they are notbased on immuno-affinity, all of our products can be used for anyspecies”states SwapanRoy, Ph.D., President and Founder of Biotech Support Group.

Clickhere for more information on NuGel™ HemogloBind™

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Keywords:tumorinterstitial fluid,tumour-associatedmacrophages,amino acid analysis, NuGel™ HemogloBind™,hemoglobin removal

AboutBiotech Support Group LLC

Convergingwith technological disruptions forthcoming in healthcare, BiotechSupport Group continues its growth in the consumable researchproducts area supporting the rapidly expanding installation of LC-MSinstrument and computational infrastructure. For this market, keyproducts include: AlbuVoid™ and AlbuSorb™ for albumin depletion,Cleanascite™ for lipid adsorption, HemogloBind™ and HemoVoid™for hemoglobin removal, For more information, gotohttp://www.biotechsupportgroup.com.

ForBusiness Development,contact:MatthewKuruc 732-274-2866, [email protected]