News Release

Research Articles Cites HemogloBind™ in Proteomic Analysis of Intestinal Tissues in Asthma Study

MONMOUTH JUNCTION, NJ, June 10, 2025 -- Biotech Support Group reports on a journal article describing the simplicity and efficiency of HemogloBind™, to remove Hemoglobin prior to LC-MS/MS proteomic analysis of intestinal tissues.

Liu, Yi-Hsuan, et al. "ITIH4 alleviates OVA-induced asthma by regulating lung-gut microbiota." Molecular Medicine 31 (2025): 204.

Inter-alpha-trypsin inhibitor heavy chain 4 (ITIH4), a Type 2 acute phase protein, is critical for resolving inflammation and promoting tissue repair. While its role in chronic respiratory diseases is recognized, its effects on asthma remain unclear. This study investigated the effects of ITIH4 on the modulation of lung and gut microbiota, the attenuation of allergic inflammation, and the improvement of respiratory outcomes in an asthma mouse model. Proteomic profiling of intestinal tissues was conducted to identify ITIH4-associated signaling pathways. For this, the article states “The intestine tissue lysate from individual mouse was subjected to hemoglobin removal by using Hemoglobind (Biotech Support Group LLC, NJ, USA). Proteomic analysis of intestinal tissues identified 4,724 proteins, with 971 and 765 differentially expressed proteins in the low- and high-dose ITIH4 groups, respectively. The study concludes that ITIH4 ameliorates asthma symptoms by modulating lung and gut microbiota, dampening Th2-driven inflammation, and restoring mucosal immune balance. These findings support ITIH4 as a potential candidate for microbiome-targeted asthma therapy. 

“For proteomics, it is necessary to efficiently remove Hemoglobin but still recover the vast majority of the remaining proteome for LC-MS analysis. This is an excellent example of how our Hemoglobin depletion product, HemogloBind™, contributed to differential proteome analysis of tissues, and that ultimately contributed to the conclusions of this study.” states Swapan Roy, Ph.D., President and Founder of Biotech Support Group.

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Keywords: Hemoglobin depletion, HemogloBind™, LC-MS/MS, tissue proteomics, Airway hyperresponsiveness, Inflammation, Asthma

About Biotech Support Group LLC

Converging with cultural and technological disruptions forthcoming in healthcare, Biotech Support Group develops methods for cost effective and efficient sample prep essential for expanding proteomic analysis. Following a tiered business strategy, the company continues its growth in the consumable research products area. For this market, key products include: AlbuVoid™ and AlbuSorb™ PLUS for albumin & IgG depletion, Cleanascite™ for lipid adsorption, HemogloBind™ and HemoVoid™ for hemoglobin removal, and NRicher™ for low abundance and family specific, and targeted proteome enrichment. For more information, go to http://www.biotechsupportgroup.com

For business development contact: Matthew Kuruc
732-274-2866 mkuruc@biotechsupportgroup.com