Plasma/Serum Protein Biomarkers
The authors aimed at simultaneously measuring intact endogenous-insulin and derived C-peptide, to help predict development of diabetes mellitus, as well as in differential diagnosis in cases of hypoglycemia. Cleanascite™ is shown both to improve LC-MS measurements, and validated in accordance with CLIA '88 guidelines. { doi: 10.1016/j.cca.2016.01.019 }
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Vaccine Development
To evaluate immunogenic response to a vaccine candidate, it is necessary to measure the antibodies from sera; a sample with a diverse lipid profile. In this citation, Cleanascite™ was used in a toxin neutralizing assay to evaluate the influence of cholesterol dependency, on a candidate protein pneumococcal vaccine. { doi: 10.1016/j.vaccine.2012.11.005 }
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Bile Proteomics
The authors report methods to overcome the biological variability of analyzing a high number of bile samples. They concluded that delipidation yielded a considerable number of complementary protein identifications and that Cleanascite™ treatment was indispensable for in-solution digestion methods. { http://dx.doi.org/10.1016/j.jprot.2016.11.021 }
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Ascites Monoclonal Antibodies
The researchers determined the role of complement on MAb-mediated protection for four mice Ig subclasses. After centrifugation of ascites fluid, Cleanascite™ protocol was implemented to remove lipids. { doi: 10.1128/IAI.70.5.2598-2604.2002 }
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Cellular Response Applications
The applications and references for the many diverse investigations using Cleanascite™ upstream of cell response measurements are described in a downloadable whitepaper. { Cleanascite Cell Response Reference Applications }
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Lipid Factors and Cancer Cell Aggressiveness
Cleanascite™️ helped this study show that the metabolite methylmalonic acid (MMA) complexed with lipidic structures, is a circulatory serum factor that contributes to pro-aggressive effects from an older population in cancer, The authors conclude that lipid-complexed MMA drives a more aggressive phenotype in cancer cells and as such, is a promising therapeutic target for advanced carcinomas.
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