News Release

Research Article Describes the Use of Cleanascite™ In the Purification of HDL-Bound small RNAs

MONMOUTH JUNCTION, NJ, January 12, 2023 -- Biotech Support Group reports on a research article describing the simplicity and efficiency of their lipid clearance sample preparation technology in a two step purification method for High-density lipoproteins (HDLs) in order to measure transport amounts of small RNAs . The citation is: 

Castleberry, Mark, et al. "High-density lipoproteins mediate small RNA intercellular communication between dendritic cells and macrophages." Journal of Lipid Research (2023): 100328.

HDL are dynamic transporters of diverse molecular cargo and play critical roles in lipid metabolism and inflammation. HDL have also been reported to accept functional small RNAs (sRNAs) from donor macrophages and deliver them to recipient endothelial cells. The present study aims to overcome these technical barriers and further understand the pathways involved in HDL-mediated bidirectional flux of sRNAs between immune cells. While the authors ultimately chose to use density gradient ultracentrifugation (DGUC) in purifying HDL, an alternate purification was considered. The authors state the use of Cleanascite™ for purification of HDL containing sRNA: “No significant differences between total HDL RNA cargo were observed between HDL purified by Size Exclusion Chromatography (SEC), followed by Cleanascite purification, and HDL isolated by DGUC.”; The article concludes that HDL is a substantive mediator of intercellular communication between immune cells and demonstrates the importance of endocytosis for recipient cells of HDL-sRNAs.

“Here is another reference showing the versatility of Cleanascite™, as a very specific lipid-binding reagent for the many varied applications that require a precise binding of lipid-macromolecules such as HDL, in this case provided by size exclusion chromatography, from the non-lipid bound protein fraction,” states Swapan Roy, Ph.D., President and Founder of Biotech Support Group.

For more information visit: Cleanascite™ Lipid Removal Reagent and Clarification, at
https://www.biotechsupportgroup.com/Lipid-Removal-s/316.htm

Keywords: High-density lipoproteins (HDLs), small RNAs (sRNAs), Cleanascite™

About Biotech Support Group LLC

Converging with cultural and technological disruptions forthcoming in healthcare, Biotech Support Group develops methods for cost effective and efficient sample prep essential for these expanding markets. Following a tiered business strategy, the company continues its growth in the consumable research products area supporting the rapidly expanding installation of LC-MS instrument and computational infrastructure. For this market, key products include: AlbuVoid™ and AlbuSorb™ for albumin depletion, Cleanascite™ for lipid adsorption, HemogloBind™ and HemoVoid™ for hemoglobin removal. From these innovations, the company has acquired knowledgebase and biomarker intellectual property assets that support discoveries of protein markers from blood, with special emphasis on early detection and personalized medical decisions for cancer patients. For more information, go to http://www.biotechsupportgroup.com.

For Business Development, contact: Matthew Kuruc 732-274-2866, mkuruc@biotechsupportgroup.com