News Release

ResearchArticle Describes the Use of Cleanascite™ In the Purification ofHDL-Bound small RNAs

MONMOUTHJUNCTION, NJ,January12, 2023 -- Biotech Support Group reports on a research articledescribing the simplicity and efficiency of their lipid clearancesample preparation technology in a two step purification method forHigh-density lipoproteins (HDLs) in order to measure transportamounts of small RNAs .The citation is: 

Castleberry,Mark, et al. "High-densitylipoproteins mediate small RNA intercellular communication betweendendritic cells and macrophages." Journalof Lipid Research (2023):100328.

HDLare dynamic transporters of diverse molecular cargo and play criticalroles in lipid metabolism and inflammation. HDL have also beenreported to accept functional small RNAs (sRNAs) from donormacrophages and deliver them to recipient endothelial cells. Thepresent study aims to overcome these technical barriers and furtherunderstand the pathways involved in HDL-mediated bidirectional fluxof sRNAs between immune cells. While the authors ultimately chose touse density gradient ultracentrifugation (DGUC) in purifying HDL, analternate purification was considered. Theauthors state the use of Cleanascite™ for purification of HDLcontaining sRNA: “No significant differences between total HDL RNAcargo were observed between HDL purified by Size ExclusionChromatography (SEC), followed by Cleanascite purification, and HDLisolated by DGUC.”; The article concludes that HDL is a substantivemediator of intercellular communication between immune cells anddemonstrates the importance of endocytosis for recipient cells ofHDL-sRNAs.

“Hereis another reference showing the versatility of Cleanascite™, as avery specific lipid-binding reagent for the many varied applicationsthat require a precise binding of lipid-macromolecules such as HDL,in this case provided by size exclusion chromatography, from thenon-lipid bound protein fraction,” states Swapan Roy, Ph.D.,President and Founder of Biotech Support Group.

Formore information visit: Cleanascite™Lipid Removal Reagent and Clarification,at
https://www.biotechsupportgroup.com/Lipid-Removal-s/316.htm

Keywords: High-density lipoproteins (HDLs), smallRNAs (sRNAs), Cleanascite™

AboutBiotech Support Group LLC

Convergingwith cultural and technological disruptions forthcoming inhealthcare, Biotech Support Group develops methods for cost effectiveand efficient sample prep essential for these expanding markets.Following a tiered business strategy, the company continues itsgrowth in the consumable research products area supporting therapidly expanding installation of LC-MS instrument and computationalinfrastructure. For this market, key products include: AlbuVoid™and AlbuSorb™ for albumin depletion, Cleanascite™ for lipidadsorption, HemogloBind™ and HemoVoid™ for hemoglobin removal.From these innovations, the company has acquired knowledgebase andbiomarker intellectual property assets that support discoveries ofprotein markers from blood, with special emphasis on early detectionand personalized medical decisions for cancer patients. For moreinformation, go tohttp://www.biotechsupportgroup.com.

ForBusiness Development, contact:MatthewKuruc 732-274-2866, [email protected]