NewsRelease
ResearchArticle Cites Cleanascite™ To Study Oocyte Cryosurvival
MONMOUTHJUNCTION, NJ,June27, 2018 -- Biotech Support Group reports on a recent researcharticle describing the simplicity and efficiency of their lipidbinding sample preparation technology for studying the toleranceof oocytes to chilling injuries when cytoplasmic lipid content isreduced.The citation is:
BarreraN, dos Santos Neto PC, Cuadro F, Bosolasco D, Mulet AP, Crispo M, etal. (2018) Impact of delipidated estrous sheep serum supplementationon invitromaturation, cryotolerance and endoplasmic reticulum stress geneexpression of sheep oocytes. PLoS ONE 13(6): e0198742.https://doi.org/10.1371/journal.pone.0198742
Highlipid content of oocytes and embryos in domestic animals is one ofthe well-known factors associated with poor cryosurvival. In thisarticles, the authors wanted to determine whether the use ofdelipidated estrous sheep serum during invitromaturation (IVM) of ovine oocytes reduces the cytoplasmic lipiddroplets content and improves embryo development and cryotoleranceafter vitrification. Thearticle states “Lipidremoval from serum was performed by using Cleanascite™ (BiotechSupport Group, NJ, USA) according to the instructions provided by themanufacturer. Unlike other approaches, the protocol described hereinfor delipidation of estrous sheep serum was effective in decreasinglevels of Triglycerides, total Cholesterol, and NEFAs. To ourknowledge this is the first study to use the Cleanascite method togenerate estrous sheep serum yielding significantly reduced lipidlevels. Subsequent use of the partially delipidated serum assupplemented in IVM media resulted in effective reduction of oocytelipid content. The advantage of this method over other traditionalmethods (i.e. chloroform) includes increased feasibility and reducedtoxicity and biosafety concerns”Their resultsdemonstrate that although supplementation of IVM medium withdelipidated estrous sheep serum reduces the presence of cytoplasmiclipid droplets in oocytes after maturation, oocyte cryotolerance isnot improved.
“Iam pleased to see another example of the versatility of Cleanascite™.This demonstrates the advantage of using an aqueous suspensionproduct with no harmful solvents, to deplete lipid associated factorsthat may influence cell response investigations.” states SwapanRoy, Ph.D., President and Founder of Biotech Support Group.
Formore information visit: Cleanascite™Lipid Removal Reagent and Clarification,at
http://www.biotechsupportgroup.com/Cleanascite-Lipid-Removal-Reagent-p/x2555.htm
AboutBiotech Support Group LLC
Convergingwith cultural and technological disruptions forthcoming inhealthcare, Biotech Support Group develops methods for cost effectiveand efficient sample prep essential for these expanding markets.Following a tiered business strategy, the company continues itsgrowth in the consumable research products area supporting therapidly expanding installation of LC-MS instrument and computationalinfrastructure. For this market, key products include: AlbuVoid™and AlbuSorb™ for albumin depletion, Cleanascite™ for lipidadsorption, HemogloBind™ and HemoVoid™ for hemoglobin removal,and NuGel™ for functional proteomics. From these innovations, thecompany has acquired knowledgebase and biomarker intellectualproperty assets that support discoveries of protein markers fromblood, with special emphasis on early detection and personalizedmedical decisions for cancer patients. For more information, go tohttp://www.biotechsupportgroup.com
Contact:
MatthewKuruc 732-274-2866