Research Article cite Cleanascite™ in Preparation of Lipid-free Cell Culture Media
Biotech Support Group reports on a journal article describing the simplicity and efficiency of using Cleanascite™ to prepare and contrast lipid-free cell culture media for evaluating Bacterial vaginosis (BV).

News Release


Research Article cite Cleanascite™ in Preparation of Lipid-free Cell Culture Media


MONMOUTH JUNCTION, NJ, August 26, 2024 -- Biotech Support Group reports on a journal article describing the simplicity and efficiency of using Cleanascite™ to prepare and contrast lipid-free cell culture media for evaluating Bacterial vaginosis (BV).

The citation is:

Zhu, Meilin, et al. "Vaginal Lactobacillus fatty acid response mechanisms reveal a metabolite-targeted strategy for bacterial vaginosis treatment." Cell (2024).


Bacterial vaginosis (BV), a common syndrome characterized by Lactobacillus-deficient vaginal microbiota, is associated with adverse health outcomes. BV often recurs after standard antibiotic therapy in part because antibiotics promote microbiota dominance by Lactobacillus iners instead of Lactobacillus crispatus, which has more beneficial health associations. Strategies to promote L. crispatus and inhibit L. iners are thus needed. This research shows that oleic acid (OA) and similar long-chain fatty acids simultaneously inhibit L. iners and enhance L. crispatus growth. The article states “To prepare lipid-depleted MRS+CQ broth, four parts MRS+CQ broth was combined with one part Cleanascite™ Lipid Removal Reagent (Biotech Support Group #X2555-10) and shaken (220 rpm) at room temperature for 10 min, then centrifuged at 2,000 x g for 15 min. The media supernatant was collected and re-sterilized using a 0.22 uM PES filter.” The article concludes that Lactobacillus OA response genes are robustly active in the human vaginal microbiome, and OA and related metabolites may improve current BV therapies We now have over thirty references, showing Cleanascite™ use in helping to identify a characteristic feature of in vitro cell response. Unlike alternative lipid-depletion methods that use solvents, Cleanascite™ is an aqueous suspension product and so it is very compatible with cellular models of disease. As shown in this article, the essential requirement is the maintenance of cellular activity after lipid depletion, so as to determine whether lipid factors are associated with phenotypic changes in cultured cells” states Swapan Roy, Ph.D., President and Founder of Biotech Support Group.


Download whitepaper entitled “ Cleanascite™ - Lipid Removal and Cell Response Applications


For more information visit: Cleanascite™ Lipid Removal Reagent and Clarification, at
http://www.biotechsupportgroup.com/Cleanascite-Lipid-Removal-Reagent-p/x2555.htm


Keywords: Cleanascite™, lipid-free media, vaginal microbiome, bacterial vaginosis


About Biotech Support Group LLC

Converging with cultural and technological disruptions forthcoming in healthcare, Biotech Support Group develops methods for cost effective and efficient sample prep essential for expanding proteomic analysis. Following a tiered business strategy, the company continues its growth in the consumable research products area. For this market, key products include: AlbuVoid™ and AlbuSorb™ PLUS for albumin & IgG depletion, Cleanascite™ for lipid adsorption, HemogloBind™ and HemoVoid™ for hemoglobin removal, and NRicher™ for low abundance and family specific proteome enrichment. For more information, go to http://www.biotechsupportgroup.com


For business development contact: Matthew Kuruc
732-274-2866 mkuruc@biotechsupportgroup.com