NewsRelease
ResearchArticle cite Cleanascite™ in Preparation of Lipid-free Cell CultureMedia
MONMOUTHJUNCTION, NJ, August26, 2024 -- Biotech Support Group reports on a journal articledescribing the simplicity and efficiency of using Cleanascite™ toprepare and contrast lipid-free cell culture media for evaluatingBacterial vaginosis (BV).
Thecitation is:
Zhu,Meilin, et al. "VaginalLactobacillus fatty acid response mechanisms reveal ametabolite-targeted strategy for bacterial vaginosistreatment." Cell (2024).
Bacterialvaginosis (BV), a common syndrome characterizedby Lactobacillus-deficientvaginal microbiota, is associated with adverse health outcomes. BVoften recurs after standard antibiotic therapy in part becauseantibiotics promote microbiota dominance by Lactobacillusiners insteadof Lactobacilluscrispatus,which has more beneficial health associations. Strategies topromote L. crispatus andinhibit L. iners arethus needed. This research shows that oleic acid (OA) and similarlong-chain fatty acids simultaneously inhibit L. iners andenhance L. crispatus growth. Thearticle states “To prepare lipid-depleted MRS+CQ broth, four partsMRS+CQ broth was combined with one part Cleanascite™ Lipid RemovalReagent (Biotech Support Group #X2555-10) and shaken (220 rpm)at room temperature for 10 min, then centrifuged at 2,000 x gfor 15 min. The media supernatant was collected andre-sterilized using a 0.22 uM PES filter.” The article concludesthat LactobacillusOA response genes are robustly active in the human vaginalmicrobiome, and OA and related metabolites may improve current BVtherapies “Wenow have over thirty references, showing Cleanascite™ use inhelping to identify a characteristic feature of invitro cellresponse. Unlike alternative lipid-depletion methods that usesolvents, Cleanascite™ is an aqueous suspension product and so itis very compatible with cellular models of disease. As shown in thisarticle, the essential requirement is the maintenance of cellularactivity after lipid depletion, so as to determine whether lipidfactors are associated with phenotypic changes in cultured cells”states Swapan Roy, Ph.D., President and Founder of Biotech SupportGroup.
Downloadwhitepaper entitled “ Cleanascite™- Lipid Removal and Cell Response Applications ”
Formore information visit: Cleanascite™ Lipid Removal Reagent andClarification, at
http://www.biotechsupportgroup.com/Cleanascite-Lipid-Removal-Reagent-p/x2555.htm
Keywords:Cleanascite™,lipid-free media, vaginal microbiome, bacterial vaginosis
AboutBiotech Support Group LLC
Convergingwith cultural and technological disruptions forthcoming inhealthcare, Biotech Support Group develops methods for cost effectiveand efficient sample prep essential for expanding proteomic analysis.Following a tiered business strategy, the company continues itsgrowth in the consumable research products area. For this market, keyproducts include: AlbuVoid™ and AlbuSorb™ PLUS foralbumin & IgG depletion, Cleanascite™for lipid adsorption, HemogloBind™ and HemoVoid™ for hemoglobinremoval, and NRicher™ for low abundance and family specificproteome enrichment. For more information, go tohttp://www.biotechsupportgroup.com
Forbusiness development contact: MatthewKuruc
732-274-2866[email protected]