NewsRelease
ResearchReport Cites Cleanascite™ for Depleting Membrane Proteins
MONMOUTHJUNCTION, NJ,March1, 2018 -- BiotechSupport Group reports on a recent research report describing thesimplicity and efficiency of their lipid clearance sample preparationtechnology for depleting the influence of pancreas membrane fractionsin the isolation of endoplasmic reticulum fractions.
Thearticle citation is:
Waldron,Richard T., et al. "EthanolInduced Disordering of Pancreatic Acinar Cell Endoplasmic Reticulum:an ER Stress/Defective Unfolded Protein Response Model."Cellularand Molecular Gastroenterology and Hepatology(2018).
Theauthors’ investigated the association of heavy alcoholconsumption with pancreas damage, with consideration of lightdrinking that shows the opposite effects, reinforcing proteostasisthrough the unfolded protein response orchestrated by X-box bindingprotein 1. The article states that the endoplasmic reticulum “RERfractions (100 mg) were extracted by shaking for 15 minutes at 37°Cin buffer comprising 100 mmol/L Tris pH 7.0, containing 25%acetonitrile, 0.1% sodium dodecyl sulfate, and 0.05% sodiumdeoxycholate (Buffer B), in 1.5 mL Eppendorf Protein LoBind tubesflushed of air using N2gas. Next, 75 mL of a 1:1 slurry of Cleanascite delipidizing beadswere added, and the tubes were flushed with N2gas and rotated end-over-end for 15 minutes at room temperature.Slurries were spun at 10,000 rpm for 5 minutes at room temperature,clarified extracts were transferred to clean tubes, and pelletedbeads were discarded.” The isolated ER proteome was thenanalyzed by OXICATLC-MS.Thestudy concludes that ethanol-induced changes in endoplasmic reticulumprotein redox and structure/function emerge from an unfolded proteinresponse–deficient genetic model.
“Thisreference shows the versatility of Cleanascite™to efficiently remove lipids from pancreas tissue extracts, so thatcellular compartments can be investigated.” states Swapan Roy,Ph.D., President and Founder of Biotech Support Group.
Formore information visit: Cleanascite™Lipid Removal Reagent and Clarification,at
http://www.biotechsupportgroup.com/Cleanascite-Lipid-Removal-Reagent-p/x2555.htm
AboutBiotech Support Group LLC
Convergingwith cultural and technological disruptions forthcoming inhealthcare, Biotech Support Group develops methods for cost effectiveand efficient sample prep essential for these expanding markets.Following a tiered business strategy, the company continues itsgrowth in the consumable research products area supporting therapidly expanding installation of LC-MS instrument and computationalinfrastructure. For this market, key products include: AlbuVoid™and AlbuSorb™ for albumin depletion, Cleanascite™ forlipid adsorption, HemogloBind™ and HemoVoid™ forhemoglobin removal, and NuGel™ for functional proteomics. Fromthese innovations, the company has acquired knowledgebase andbiomarker intellectual property assets that support discoveries ofprotein markers from blood, with special emphasis on early detectionand personalized medical decisions for cancer patients. For moreinformation, go tohttp://www.biotechsupportgroup.com
Contact:
MatthewKuruc 732-274-2866
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