NewsRelease

Cleanascite™Employed to Determine Role of Lipid-Associated Factors inMyofibroblast Conversion

MONMOUTHJUNCTION, NJ, December12, 2023 -- Biotech Support Group reports on an article, describingthe simplicity and efficiency of their lipid clearance samplepreparation technology to determine the role of lipid-associatedfactors in adipocyte signaling and fibroblastto myofibroblast conversion.The citation is:


El-Hattab,Mariam Y., et al. "Nativeadiponectin plays a role in the adipocyte-mediated conversion offibroblasts to myofibroblasts." Journalof the Royal Society interface 20.202(2023): 20230004.

Adipocytesregulate tissues through production of adipokines that can act bothlocally and systemically, playing a critical role in regulating thehealing process. The authors sought to identify how mature adipocytessignal to dermal fibroblasts through adipokines to inducemyofibroblast conversion. By using molecular weight fractionation,heat inactivation and lipid depletion, they determined matureadipocytes secrete a factor that is 30–100 kDa, heat labile andlipid associated that induces myofibroblast conversion. Cleanascitewas used as stated: “To determine if the factor is a protein, weheat inactivated (HI) the adipocyte-conditionedmedia(ACM) and found that doing socompletely eliminated the ability of ACM to induce α-SMA expression(figure 3a). Finally, to determine if the factor is lipid associated,we depleted lipids (LD) and lipid-associated factors usingCleanascite and found the α-SMA inducing activity of ACM was alsoeliminated (figure 3a).When fractionation, heat inactivation or lipid depletion wasperformed on TGF-β1 containing positive control media, only the lessthan 30 kDa fraction and lipid-depleted ACM retained α-SMA inducingactivity, findings completely expected and consistent with thephysico-chemical properties of TGF-β1 (the active form of TGF-β1 isa 25 kDa dimer).”.

Theauthors conclude that adiponectin secreted by mature adipocytesinduces fibroblast to myofibroblast conversion and may lead to aphenotype of myofibroblasts distinct from TGF-β1-inducedmyofibroblasts.

“Wenow have over thirty references, showing Cleanascite™ use inhelping to identify a characteristic feature of invitrocell response. Unlike alternative lipid-depletion methods that usesolvents, Cleanascite™ is an aqueous suspension product and so itis very compatible with cellular models of disease. The essentialrequirement is that it maintains cellular activity, while selectivelydepleting lipid factors. This ultimately helps with investigationssuch as this, to determine whether a secreted factor islipid-associated or not” states Swapan Roy, Ph.D., President andFounder of Biotech Support Group.

Downloadwhitepaper entitled “Cleanascite™- Lipid Removal and Cell Response Applications”,visit:

Formore information visit: Cleanascite™Lipid Removal Reagent and Clarification,at
http://www.biotechsupportgroup.com/Cleanascite-Lipid-Removal-Reagent-p/x2555.htm

Keywords:Lipiddepletion, Cleanascite™, Adipocytes, adipokines, myofibroblastconversion, TGF-β

AboutBiotech Support Group LLC

Convergingwith cultural and technological disruptions forthcoming inhealthcare, Biotech Support Group develops methods for cost effectiveand efficient sample prep essential for these expanding markets.Following a tiered business strategy, the company continues itsgrowth in the consumable research products area supporting therapidly expanding installation of LC-MS instrument and computationalinfrastructure. Forthis market, key products include: AlbuVoid™ and AlbuSorb™ PLUSforalbumin & IgG depletion, Cleanascite™for lipid adsorption, HemogloBind™ and HemoVoid™ for hemoglobinremoval, and NRicher™ for low abundance and family specificproteome enrichment.For more information, go tohttp://www.biotechsupportgroup.com.

ForBusiness Development,contact:MatthewKuruc 732-274-2866, [email protected]