Blood is the vehicle for the accumulative evidence for all pathological insults – both infectious and non-infectious. Information derived from blood can be compartmentalized into 4 major categories: Plasma, Leukocytes (especially Neutrophils), Platelets, and Erythrocytes.Our products are used to generate proteomic and other ‘omics’ information from all these compartments.
Hemoglobin accounting for ≥ 95% of the protein mass in erythrocytes, is often an interfering factor for many types of analysis, both proteinaceous and non-proteinaceous (i.e., PCR). With the rising use of dried blood cards as a cost effective and transportable sample type, analytical interferences from Hemoglobin continues to present problems. Our specialized application specific products are designed for all these challenges!
Sample prep methods essential for expanding proteomic biomarkers into routine healthcare.
Supporting the expanding installation of LC-MS instrument & computational infrastructure.
Not derived from immuno-affinity
All products work for all species
Mass Spectrometry (LC-MS & MALDI),
Immunoassays, ELISAs & Western Blots,
1 & 2 DE,
Enzyme & Functional Assays
Removes Hemoglobin from erythrocyte and whole blood lysates, enriching the low abundance proteome
LEARN MOREDepletion plus low abundance protein enrichment with optimized on-bead digestion for LC-MS erythrocyte & whole blood proteomics.
LEARN MOREThe HemoVoid™ Blood Card kit substantially reduces hemoglobin interference from dried blood spot protein analytes
LEARN MOREPurification & Enrichment Of Hemoglobin From Blood For Hemoglobin Variant Research
LEARN MOREEngineered for high degree of selectivity to avoid cross reacting with most common serum components.
LEARN MORESimilar performance properties to HemogloBind suspension, but supplied in dry powder format.
LEARN MOREHemoglobin Depletion and Protein Enrichment From Dried Blood Spots
LEARN MOREInterested in learning more about our complete portfolio of Hemoglobin Removal products?
LEARN MOREThere have been 40+ citations for successful use of our products for a variety of diseases and challenging applications for hemoglobin removal. Here are some of the highlights.
HemoVoid™ “protein-level pre-fractionation proved helpful in identifying additional proteins and N-termini”. 778 proteins were identified from the cytosolic fraction, 171 of which were not represented in either the soluble non-depleted fraction or the membrane fraction.
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The analysis of Plasmodium falciparum schizont phospho-proteome followed HemoVoid™ treatment.
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As the extremely high abundance of hemoglobin from erythrocytes can often interfere with protein analysis, the article states PSer129 α -Syn & Oxidized α -Syn detection: “followed from hemoglobin clearance with HemoVoid kit…”.
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After hemoglobin depletion using HemogloBind™, immunoblot analysis identified that PARK4 mutation patient blood showed upregulation of alpha-synuclein monomer, with no high molecular weight aggregates.
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Careful investigation of different strategies for … proteome profiling by 2-DE was carried out, paying particular attention to hemoglobin removal. The paper concludes that “a simple, quick, and satisfactory approach for hemoglobin depletion of erythrocyte cells based on HemogloBind™ reagent is shown here to satisfactorily analyze the cytosolic sub-proteome by 2-DE without major interference.”
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The article investigates Total cholinesterase activity of whole blood samples with HemogloBind™ treatment prior to Ellman method. It concludes that, the HemogloBind™ protocol is consistent, and simple with only one incubation and a short, low speed centrifugation step.
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From a Science article, the study used multiplexed quantitative proteomics to identify candidate substrates of UBE2O, an E2 (ubiquitin-conjugating) enzyme, in an unbiased and global manner. Because of the overly abundant presence of Hemoglobin, selective depletion of using HemogloBind™ was employed.
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published April 28th, 2020
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