Key Applications:
There have been 40+ citations for successful use of our products for a variety of diseases and challenging applications for hemoglobin removal. Here are some of the highlights.
Human Proteome Project - Annotation of Erythrocytes
HemoVoid™ “protein-level pre-fractionation proved helpful in identifying additional proteins and N-termini”. 778 proteins were identified from the cytosolic fraction, 171 of which were not represented in either the soluble non-depleted fraction or the membrane fraction.
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Malaria infected erythrocytes
The analysis of Plasmodium falciparum schizont phospho-proteome followed HemoVoid™ treatment.
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Parkinson’s Disease
As the extremely high abundance of hemoglobin from erythrocytes can often interfere with protein analysis, the article states PSer129 α -Syn & Oxidized α -Syn detection: “followed from hemoglobin clearance with HemoVoid kit…”.
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After hemoglobin depletion using HemogloBind™, immunoblot analysis identified that PARK4 mutation patient blood showed upregulation of alpha-synuclein monomer, with no high molecular weight aggregates.
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Erythrocyte Proteomic Methods
Careful investigation of different strategies for … proteome profiling by 2-DE was carried out, paying particular attention to hemoglobin removal. The paper concludes that “a simple, quick, and satisfactory approach for hemoglobin depletion of erythrocyte cells based on HemogloBind™ reagent is shown here to satisfactorily analyze the cytosolic sub-proteome by 2-DE without major interference.”
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Whole Blood Lysates
The article investigates Total cholinesterase activity of whole blood samples with HemogloBind™ treatment prior to Ellman method. It concludes that, the HemogloBind™ protocol is consistent, and simple with only one incubation and a short, low speed centrifugation step.
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Reticulocyte transitioning
From a Science article, the study used multiplexed quantitative proteomics to identify candidate substrates of UBE2O, an E2 (ubiquitin-conjugating) enzyme, in an unbiased and global manner. Because of the overly abundant presence of Hemoglobin, selective depletion of using HemogloBind™ was employed.
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