News Release

Journal Article Cites HemoVoid™ for Proteome Profiling of Dairy Buffalo Red Cells

MONMOUTH JUNCTION, NJ , March 6, 2025 -- Biotech Support Group reports on a research article describing the simplicity and efficiency of their HemoVoid™ technology for enriching erythrocyte low abundance proteins, in order to assess heat stress in dairy buffaloes.

The citation is:

Lan, Qin, and Zhao-bing Gu. " Data-independent acquisition-based proteome profiling of red blood cells from dairy buffaloes under different types of heat stress ." Veterinary and Animal Science (2025): 100437.

Heat stress (HS) induces hypoxia and oxidative stress, reducing animal health and livestock production. Red Blood Cell (RBC) lysates were isolated for data-independent acquisition-based proteomics to identify differentially expressed proteins involved in the HS response. The article states “HemoVoid™ LC-MS On-Bead kit (Biotech Support Group, HVB-MS10) was used to remove Hb”. Digested peptides were analyzed by nanometric high performance liquid chromatography performed with a Q-Exactive HFX (Thermo Scientific) mass spectrometer, operated in DIA mode. Results showed that blood clotting factors, complement proteins, immunoglobulins, and vasoconstriction proteins in RBCs were consistently decreased under the three types of HS conditions. 

“Many references now validate the use of HemoVoid™ as an efficient method to deplete Hemoglobin in order to enrich and analyze low abundance sub-proteomes, especially from red cell lysates. This article also highlights that our products are species agnostic, suitable for all animal studies”, states Swapan Roy, Ph.D., President and Founder of Biotech Support Group. 

For more information on HemoVoid™, visit:

http://www.biotechsupportgroup.com/HemoVoid-Hemoglobin-Depletion-From-Erythrocytes-p/hvk.htm

For more information of all of our Hemoglobin removal products, visit:

https://www.biotechsupportgroup.com/Hemoglobin-Removal-s/312.htm

About Biotech Support Group LLC

Converging with cultural and technological disruptions forthcoming in healthcare, Biotech Support Group develops methods for cost effective and efficient sample prep essential for expanding proteomic analysis. Following a tiered business strategy, the company continues its growth in the consumable research products area. For this market, key products include: AlbuVoid™ and AlbuSorb™ PLUS for albumin & IgG depletion, Cleanascite ™ for lipid adsorption, HemogloBind™ and HemoVoid™ for hemoglobin removal, and NRicher™ for low abundance, family specific, and targeted proteome enrichment. For more information, go to http://www.biotechsupportgroup.com

For business development contact: Matthew Kuruc
732-274-2866 mkuruc@biotechsupportgroup.com

Keywords

Data-independent acquisition (DIA) LC-MS, Dairy buffalo, Heat stress, RBC lysates, Hemoglobin depletion, erythrocyte proteomics, HemoVoid™