NewsRelease
Cleanascite™Employed to Determine Role of Lipids in Neutrophil Trafficking toInfection Sites
MONMOUTHJUNCTION, NJ, December19, 2022 -- Biotech Support Group reports on an article, describingtheir lipid clearance sample preparation technology to help determinethe role of fatty acid oxidation and mitochondrial metabolismin a cell response study of murine cell culture models ofsusceptibility to infection. The citation is:
Pham,Ly, et al. "Neutrophiltrafficking to the site of infection requires Cpt1a-dependent fattyacid β-oxidation." CommunicationsBiology 5.1(2022): 1-13.
Carnitinepalmitoyltransferase 1a (Cpt1a) is considered the rate-limitingenzyme for mitochondrial metabolism of long-chain fatty acids, andCpt1a deficiency is associated with infant mortality and infectionrisk. This study was undertaken to test the hypothesis thatimpairment in Cpt1a-dependent fatty acid oxidation results inincreased susceptibility to infection. Thearticle states for lipid depletion “…cells were incubatedwith RPMI containing 0.5% FBS or with 10% FBS that had been treatedwith Cleanascite (Biotech Support Group) to remove lipids”.Observations were made that manipulation of exogenous free fattyacids, either through the depletion of lipids from the culture mediumor through the addition of exogenous octanoic acid, alters neutrophilchemotactic functions. Thearticle concludes that susceptibility to pneumonia isassociated with blunted neutrophilic responses in mice and humansthat result from impaired neutrophil trafficking to the site ofinfection. Chemotaxis responsible for neutrophil trafficking requiresCpt1a-dependent mitochondrial fatty acid oxidation for amplificationof chemoattractant signals. These findings identify Cpt1a as apotential host determinant of infection susceptibility anddemonstrate a requirement for mitochondrial fatty acid oxidation inneutrophil biology.
“Wenow have over twenty references, showing that Cleanascite™ was usedto help identify a characteristic feature of invitrocell response. Unlike alternative lipid-depletion methods that usesolvents, Cleanascite™ is an aqueous suspension product and so itis very compatible with cellular models of disease. This ultimatelyhelps with investigations such as this, whereby the cells requirefetal bovine serum for growth, but the exogenous lipids need to beremoved in order to determine their phenotypic effect.” statesSwapan Roy, Ph.D., President and Founder of Biotech Support Group.
Todownload whitepaper entitled “Cleanascite™- Lipid Removal and Cell Response Applications”,visit:
Formore information visit: Cleanascite™Lipid Removal Reagent and Clarification,at
http://www.biotechsupportgroup.com/Cleanascite-Lipid-Removal-Reagent-p/x2555.htm
Keywords
Carnitinepalmitoyltransferase 1a (Cpt1a), Neutrophil trafficking, Neutrophilchemotaxis, Cleanascite™, lipid depletion, mitochondrial fatty acidoxidation, microbial infection susceptibility
AboutBiotech Support Group LLC
Convergingwith cultural and technological disruptions forthcoming inhealthcare, Biotech Support Group develops methods for cost effectiveand efficient sample prep essential for these expanding markets.Following a tiered business strategy, the company continues itsgrowth in the consumable research products area supporting therapidly expanding installation of LC-MS instrument and computationalinfrastructure. For this market, key products include: AlbuVoid™and AlbuSorb™ for albumin depletion, Cleanascite™ for lipidadsorption, HemogloBind™ and HemoVoid™ for hemoglobin removal.From these innovations, the company has acquired knowledgebase andbiomarker intellectual property assets that support discoveries ofprotein markers from blood, with special emphasis on early detectionand personalized medical decisions for cancer patients. For moreinformation, go tohttp://www.biotechsupportgroup.com.
ForBusiness Development,contact:MatthewKuruc 732-274-2866, [email protected]