ResearchArticle Cites Cleanascite™ In Lipid Factor Determination of ImmuneResponse in Plants
MONMOUTH JUNCTION, NJ, October 7, 2020 -- Biotech Support Group reports on a journal article describing the simplicity and efficiency of their lipid clearance sample preparation technology to determine that lipid factors cause an immune response in plants. The citation is:
Stahl,Elia, et al. "Phosphatidylcholinesfrom Pieris brassicae eggs activate an immune response inArabidopsis."eLife 9 (2020): e60293.
Recognitionof conserved microbial molecules activates immune responses inplants, a process termed pattern-triggered immunity (PTI). Similarly,insect eggs trigger defenses that impede egg development or attractpredators, but information on the nature of egg-associated elicitorsis scarce. The authors performed an unbiased bioactivity-guidedfractionation of eggs of the butterfly Pierisbrassicaetodetermine the nature of egg-associated molecular patterns (EAMPs)that induce immune responses in Arabidopsis.
Preliminarytests indicated that egg-derived defense eliciting compounds are oflipidic nature. To confirm this, the authors stated “we usedCleanascite solid-phase aqueous reagent to selectively adsorb lipidsfrom EE (Egg Extract). Application of the lipid-containing phase to…Arabidopsisreporter lines triggered strong and localized GUS staining, similarto EE treatment. In contrast, the supernatant containing proteins andother non-lipidic molecules was not active, indicating that defensegene-inducing molecules were restricted to the lipid phase (Figure1A)”. This helped the research in the identification ofphosphatidylcholines (PCs) as egg-associated molecular patterns(EAMPs), and illustrated the acute ability of plants to detectconserved immunogenic patterns from their enemies, even fromseemingly passive structures such as eggs.
“Itsrewarding to see another reference showing that Cleanascite™helped to identify previously unknown lipid factors which produced animmune response in plants. As Cleanascite™is an aqueous suspension product without harmful solvents, one canuse it to challenge cells for responsiveness. This ultimately helpsresearchers decide whether or not lipids, or factors interacting withlipids, impart characteristic changes in cells.” states Swapan Roy,Ph.D., President and Founder of Biotech Support Group.
Todownload whitepaper entitled “Cleanascite™- Lipid Removal and Cell Response Applications”,visit:
Formore information visit: Cleanascite™Lipid Removal Reagent and Clarification,at
http://www.biotechsupportgroup.com/Cleanascite-Lipid-Removal-Reagent-p/x2555.htm
AboutBiotech Support Group LLC
Convergingwith cultural and technological disruptions forthcoming inhealthcare, Biotech Support Group develops methods for cost effectiveand efficient sample prep essential for these expanding markets.Following a tiered business strategy, the company continues itsgrowth in the consumable research products area supporting therapidly expanding installation of LC-MS instrument and computationalinfrastructure. For this market, key products include: AlbuVoid™and AlbuSorb™ for albumin depletion, Cleanascite™ for lipidadsorption, HemogloBind™ and HemoVoid™ for hemoglobin removal,and NuGel™ for functional proteomics. From these innovations, thecompany has acquired knowledgebase and biomarker intellectualproperty assets that support discoveries of protein markers fromblood, with special emphasis on early detection and personalizedmedical decisions for cancer patients. For more information, go tohttp://www.biotechsupportgroup.com.
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