Lipid Removal & Clarification


Cleanascite™

Lipid Adsorption & Clarification

  • Effectively replaces chlorinated/fluorinated hydrocarbons (eg. freon)
  • Workflows for antibodies, proteins, nucleic acids, proteoglycans, and most serum analytes
  • A high binding capacity for lipids with minimal cross-reactivity with proteins and nucleic acids
  • Suitable alternative to StatSpin LipoClear
  • Ideal for clarifying ascites, serum, cell & tissue culture, bile and organ homogenates
  • Clarifies saliva and fecal components
  • Exquisite selectivity profile including extracellular vesicle and exosome clearance
  • Extends the life of membrane and chromatographic columns.
  • Enrichment of delipidated tissue samples
  • For downstream processing of large-scale therapeutic proteins, enzymes and monoclonal antibodies.

Cleanascite™ is derived through a proprietary formulation of metallic oxide derivatives. Unlike other metallic oxides, Cleanascite™ does not have significant protein binding making its selectivity profile for lipids unique in the bio-research products industry. As a result, it is ideal to clear lipid-associated matrix effects from human sera, bile, ascites, and other high lipid content sample types.

View Details
Egg Yolk Clarification


Egg Yolk Clarification
Insert: PAGE showing
Left: Markers
Right: IgY and other major protein fractions recovered

Discard
Lipids
Cholesterol
Extracellular Vesicles
↑

Cleanascite™
Solid-phase Aqueous Suspension
No Solvents, Freon or Chloroform
Simple Centrifuge (Not Ultra) Protocols
Safe Disposal

↓

Improved Assay Performance
- ELISA
- Immunocapture Microarrays
- LC-MS
- Toxin Neutralizing Titer
- Cell Response

Cleanascite™ is supplied as a suspension reagent.

Cleanascite™ is supplied as a suspension reagent. Simply add, mix and centrifuge in a 10 minute protocol.


Key References

Plasma/Serum Protein Biomarkers

The authors aimed at simultaneously measuring intact insulin and proinsulin derived C-peptide, to help predict development of diabetes mellitus, as well as in differential diagnosis in cases of hypoglycemia. Cleanascite™ is shown both to improve LC-MS measurements, and validated in accordance with CLIA ’88 guidelines. { doi: 10.1016/j.cca.2016.01.019 }

Vaccine Development

To evaluate immunogenic response to a vaccine candidate, it is necessary to measure the antibodies from sera; a sample with a diverse lipid profile. In this citation, Cleanascite™ was used in a toxin neutralizing assay to evaluate the influence of cholesterol dependency, on a candidate protein pneumococcal vaccine. { doi: 10.1016/j.vaccine.2012.11.005 }

Bile Proteomics

The authors report methods to overcome the biological variability of analyzing a high number of bile samples. They concluded that delipidation yielded a considerable number of complementary protein identifications and that Cleanascite™ treatment was indispensable for in-solution digestion methods. { http://dx.doi.org/10.1016/j.jprot.2016.11.021 }

Ascites Monoclonal Antibodies

The researchers determined the role of complement on MAb-mediated protection for four mice Ig subclasses. After centrifugation of ascetic fluid, Cleanascite™ protocol was implemented to remove lipids. { doi: 10.1128/IAI.70.5.2598-2604.2002 }

Cellular Response Applications

The applications and references for the many diverse investigations using Cleanascite™ upstream of cell response measurements are described. { Cleanascite Cell Response Reference Applications }

Cleanascite™ LX

For Lipemic Serum/Plasma Clarification

  • Effectively replaces chlorinated/fluorinated hydrocarbons (eg. freon)
  • Based on solid-phase referenced in over 70 publications in varied applications
  • Under investigation as alternative to LipoClear for lipemic serum/

Protocol

Cleanascite™ LX is supplied as an aqueous suspension of non-ionic adsorbent in DI water, pH 8.0. After centrifugation, the pellet is 1/2 of the total volume and the supernatant is 1/2 of the total volume.

View Details
Cleanscite LX Diagram
  1. Resuspend CleanasciteTM LX by gentle shaking. Excessive shaking may cause foaming. It should be completely resuspended prior to use.
  2. Add CleanasciteTM LX to the sample at minimum 1:3 (or alternative higher) ratio. Mix the sample by gentle shaking for 20 minutes.
  3. Micro-centrifuge sample at 8,000 rpm’s (5,000xg) for 10 minutes.
  4. Carefully aspirate supernatant for analysis.

Optimization. Different sample volumes are easily scaled. Volume ratio can be adjusted up or down as required to remove the amount of impurities present.


Our BSG Advantage


Consumable

Cost-effective, not derived from biologicals

Cost-effective, not derived from biologicals

  • No specialized instruments or HPLC
  • Economical surface chemistries, not derived from biologicals
  • No regeneration, so no prep to prep variability
  • Simple, fast microfuge bind/wash/elute protocols

On-Bead Digestion

Efficient workflows, quality LC-MS/MS data

Efficient workflows, quality LC-MS/MS data

  • Simple, reproducible workflows
  • Equivalent or better than in-solution digestion
  • Seamless to LC-MS, no desalting or C18 separations
  • Unique proteolytic efficiencies

Enrichment/Depletion

Diverse strategies, species agnostic

Diverse strategies, species agnostic

  • Products support strategies for both enrichment of low abundance proteomes, or depletion of high abundance proteins
  • Species agnostic not derived from biologicals

Functional Integrity

Maintained throughout all separations

Maintained throughout all separation

  • Mild buffer conditions maintains native structure with retained enzymatic, functional & bio-activities
  • Supports enzyme biomarker assays
  • Functional & Chemical Proteomics
  • Structural & activity-probe Proteomics
  • Top-down & ArrayBridge PEP Proteomics