Lipid Removal & Clarification


Cleanascite™

Lipid Adsorption & Clarification

  • Effectively replaces chlorinated/fluorinated hydrocarbons (eg. freon)
  • Workflows for antibodies, proteins, nucleic acids, proteoglycans, and most serum analytes
  • A high binding capacity for lipids with minimal cross-reactivity with proteins and nucleic acids
  • Ideal for clarifying ascites, serum, cell & tissue culture, bile and organ homogenates
  • Clarifies saliva and fecal components
  • Exquisite selectivity profile
  • Extends the life of membrane and chromatographic columns.
  • Enrichment of delipidated tissue samples
  • For downstream processing of large-scale therapeutic proteins, enzymes and monoclonal antibodies.

Cleanascite™ is derived through a proprietary formulation of metallic oxide derivatives. Unlike other metallic oxides, Cleanascite™ does not have significant protein binding making its selectivity profile for lipids unique in the bio-research products industry. As a result, it is ideal to clear lipid-associated matrix effects from human sera, bile, ascites, and other high lipid content sample types.

View Details
Egg Yolk Clarification


Egg Yolk Clarification
Insert: PAGE showing
Left: Markers
Right: IgY and other major protein fractions recovered


Discard
Lipids
Cholesterol

Cleanascite™
Safe Disposal, No Freon or Chloroform



Improved Assay Performance
- ELISA
- Immunocapture Microarrays
- LC-MS
- Toxin Neutralizing Titer


Cleanascite™ is supplied as a suspension reagent.

Cleanascite™ is supplied as a suspension reagent. Simply add, mix and centrifuge in a 10 minute protocol.


Key References

Plasma/Serum Protein Biomarkers

The authors aimed at simultaneously measuring intact insulin and proinsulin derived C-peptide, to help predict development of diabetes mellitus, as well as in differential diagnosis in cases of hypoglycemia. Cleanascite™ is shown both to improve LC-MS measurements, and validated in accordance with CLIA ’88 guidelines. { doi: 10.1016/j.cca.2016.01.019 }

Vaccine Development

To evaluate immunogenic response to a vaccine candidate, it is necessary to measure the antibodies from sera; a sample with a diverse lipid profile. In this citation, Cleanascite™ was used in a toxin neutralizing assay to evaluate the influence of cholesterol dependency, on a candidate protein pneumococcal vaccine. { doi: 10.1016/j.vaccine.2012.11.005 }

Bile Proteomics

The authors report methods to overcome the biological variability of analyzing a high number of bile samples. They concluded that delipidation yielded a considerable number of complementary protein identifications and that Cleanascite™ treatment was indispensable for in-solution digestion methods. { http://dx.doi.org/10.1016/j.jprot.2016.11.021 }

Ascites Monoclonal Antibodies

The researchers determined the role of complement on MAb-mediated protection for four mice Ig subclasses. After centrifugation of ascetic fluid, Cleanascite™ protocol was implemented to remove lipids. { doi: 10.1128/IAI.70.5.2598-2604.2002 }


Our BSG Advantage


Consumable

Cost-effective, not derived from biologicals

Cost-effective, not derived from biologicals

  • No specialized instruments or HPLC
  • Economical surface chemistries, not derived from biologicals
  • No regeneration, so no prep to prep variability
  • Simple, fast microfuge bind/wash/elute protocols

On-Bead Digestion

Efficient workflows, quality LC-MS/MS data

Efficient workflows, quality LC-MS/MS data

  • Simple, reproducible workflows
  • Equivalent or better than in-solution digestion
  • Seamless to LC-MS, no desalting or C18 separations
  • Unique proteolytic efficiencies

Enrichment/Depletion

Diverse strategies, species agnostic

Diverse strategies, species agnostic

  • Products support strategies for both enrichment of low abundance proteomes, or depletion of high abundance proteins
  • Species agnostic not derived from biologicals

Functional Integrity

Maintained throughout all separations

Maintained throughout all separation

  • Mild buffer conditions maintains native structure with retained enzymatic, functional & bio-activities
  • Supports enzyme biomarker assays
  • Functional & Chemical Proteomics
  • Structural & activity-probe Proteomics
  • Top-down & ArrayBridge PEP Proteomics