News Release

Cleanascite™ Cited In Study of Alzheimer’s Disease Model of the Integrated Stress Response

MONMOUTH JUNCTION, NJ, December 30, 2024 -- Biotech Support Group reports on an article, describing the simplicity and efficiency of their lipid clearance sample preparation from integrated stress models of disease in mice. The citation is:

Flury, Anna, et al. "A neurodegenerative cellular stress response linked to dark microglia and toxic lipid secretion." Neuron (2024).

The brain’s primary immune cells, microglia, are a leading causal cell type in Alzheimer’s disease (AD). Yet, the mechanisms by which microglia can drive neurodegeneration remain unresolved. The integrated stress response (ISR), characterizes a microglia subset with neurodegenerative outcomes. Autonomous activation of ISR in microglia is sufficient to induce early features of the ultrastructurally distinct “dark microglia” linked to pathological synapse loss. In AD models, microglial ISR activation exacerbates neurodegenerative pathologies and synapse loss while its inhibition ameliorates them. Mechanistically, evidence is presented that ISR activation promotes the secretion of toxic lipids by microglia, impairing neuron homeostasis and survival in vitro. Notably, eliminating lipids using an adsorbent resin (Cleanascite) abolished these toxic effects. The article states “Lipid depletion of conditioned media was done using Cleanascite™ (Biotech Support Group, X2555) following the manufacturer’s recommendations. Briefly, 1:4 of the Cleanascite™ reagent was added to the conditioned media, incubated for 10 min at room temperature, mixed periodically, and then centrifuged at 1,600 g for 2 min. The supernatant was carefully collected, centrifuged again, and filtered through a 0.22 μm-syringe filter (Avantor, 76479-024) before applying it to recipient cells.”. The article’s results demonstrate that microglial ISR activation represents a neurodegenerative phenotype, which may be sustained, at least in part, by the secretion of toxic lipids.

“We now have over thirty references, showing Cleanascite™ use in helping to identify a lipid-based characteristic feature of in vitro cell response. Unlike alternative lipid-depletion methods that use solvents, Cleanascite™ is an aqueous suspension product and so it is very compatible with cellular models of disease. The essential requirement is the maintenance of cellular activity after lipid depletion, to determine whether lipid factors are associated with phenotypic changes in cultured cells” states Swapan Roy, Ph.D., President and Founder of Biotech Support Group.

Download whitepaper entitled “Cleanascite™ - Lipid Removal and Cell Response Applications”, visit:

For more information visit: Cleanascite™ Lipid Removal Reagent and Clarification, at
http://www.biotechsupportgroup.com/Cleanascite-Lipid-Removal-Reagent-p/x2555.htm

Keywords: microglia, dark microglia, Alzheimer’s disease, neurodegeneration, integrated stress response (ISR), lipotoxicity, Cleanascite™, lipid depletion

About Biotech Support Group LLC

Converging with cultural and technological disruptions forthcoming in healthcare, Biotech Support Group develops methods for cost effective and efficient sample prep essential for these expanding markets. Following a tiered business strategy, the company continues its growth in the consumable research products area supporting the rapidly expanding installation of LC-MS instrument and computational infrastructure. For this market, key products include: AlbuVoid™ and AlbuSorb™ PLUS for albumin & IgG depletion, Cleanascite™ for lipid adsorption, HemogloBind™ and HemoVoid™ for hemoglobin removal, and NRicher™ for targeted proteomics and family specific proteome enrichment. For more information, go to http://www.biotechsupportgroup.com. 

For Business Development, contact: Matthew Kuruc 732-274-2866, mkuruc@biotechsupportgroup.com