News Release

Research Article Cites and NRicher™ and Cleanascite™ in Map of Protein-Protein Interactions

MONMOUTH JUNCTION, NJ , June 27, 2024 -- Biotech Support Group reports on a research article, describing the simplicity and efficiency of their differential proteomic enrichment technology to observe and annotate multiprotein complexes in Toxoplasma gondii , a major food borne pathogen .

The citation is:

Swapna LS, Stevens GC, Sardinha-Silva A, Hu LZ, Brand V, Fusca DD, et al. (2024 ) ToxoNet: A high confidence map of protein-protein interactions in Toxoplasma gondii. PLoS Comput Biol 20(6): e1012208. https://doi.org/10.1371/ journal.pcbi.1012208

The apicomplexan intracellular parasite Toxoplasma gondii is a major food borne pathogen that is highly prevalent in the global population. The majority of the T. gondii proteome remains uncharacterized and the organization of proteins into complexes is unclear. To overcome this knowledge gap, a biochemical fractionation strategy was used to predict interactions by correlation profiling. To generate coelution profiles… samples were subjected to six biochemical fractionation experiments: five using beads featuring different surface chemistries for generating subproteomes based on physico-chemical similarities, see Figure. The article states we used affinity beads (NuGel PROspector) to pre-enrich Toxoplasma gondii lysate to capture five distinct subproteomes. [Note: NuGel PROspector beads are now part of the NRicher™ platform.]… In this experiment, we added one volume of Cleanascite™ [Biotech Support Group] to five volumes of the sample to remove lipids and any insoluble biomass. … The resulting elutes from the five beads were kept for later HPLC fractionation. ” The authors conclude with a roadmap, which they term ToxoNet, detailing 2,063 connections between 652 proteins. These include proteins representing parasite-specific innovations that help Toxoplasma perform essential functions such as the generation of energy. Further, in addition to capturing proteins already implicated in host cell invasion pathways, we identified many additional components of these pathways that represent novel targets for future drug development strategies.

“ I am very pleased that two of our products were able to contribute to this rigorous examination of protein complexes. When comparing the 5 different subproteomes, there is clearly different selection biases amongst the 5 surface chemistries. Also, many of the proteins observed from the NRicher™ beads, were not observed in the Ion exchange fractions demonstrating the importance of combining different modes (ionic, hydrophobic, etc.) of separation to alter selection properties, and consequently improving overall proteome coverage. This further validates that the sub-proteome bias characteristics of the NRicher™ platform can simplify complex proteomes into differentiated sub-proteomes with efficiencies suitable for deep functional proteome characterization. These features help in applications such as this whereby coverage is most important, but also in targeted applications whereby specific enrichment is most important.” states Swapan Roy, Ph.D., President and Founder of Biotech Support Group.

For more information on the NRicher™ platform, visit:

https://www.biotechsupportgroup.com/NRicher-s/329.htm

For more information visit: Cleanascite™ Lipid Removal Reagent and Clarification, at
http://www.biotechsupportgroup.com/Cleanascite-Lipid-Removal-Reagent-p/x2555.htm

Keywords: NRicher™, protein-protein interactions, subproteome enrichment, Cleanascite™, Toxoplasma gondii

About Biotech Support Group LLC

Converging with cultural and technological disruptions forthcoming in healthcare, Biotech Support Group develops methods for cost effective and efficient sample prep essential for these expanding markets. Following a tiered business strategy, the company continues its growth in the consumable research products area supporting the rapidly expanding installation of LC-MS instrument and computational infrastructure. For this market, key products include: AlbuVoid™ and AlbuSorb™ PLUS for albumin & IgG depletion, Cleanascite ™ for lipid adsorption, HemogloBind™ and HemoVoid™ for hemoglobin removal, and NRicher™ for targeted proteomics and family specific proteome enrichment. For more information, go to http://www.biotechsupportgroup.com.

For Business Development , contact: Matthew Kuruc 732-274-2866, mkuruc@biotechsupportgroup.com